CsANS1, a gene encoding anthocyanin synthase, and its applications
By cloning the tea plant ANS gene CsANS1, a recombinant engineered bacterium was constructed to achieve the synergistic biosynthesis of anthocyanins and quercetin. This solved the resource and environmental problems in the production of anthocyanins and quercetin in existing technologies, realizing efficient and green synthesis and broadening its application in metabolic engineering.
Patent Information
- Application Number
- CN202511555121.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2025-10-29
- Publication Date
- 2026-01-30
- Estimated Expiration
- 2045-10-29
AI Technical Summary
In the current technology, the production of anthocyanins and quercetin relies on plant extraction or chemical synthesis, which has problems such as limited resources, complicated steps, large environmental pollution, and difficulty in guaranteeing the natural configuration of the products. In addition, there is a lack of efficient enzyme resources and supporting processes that can simultaneously synthesize multiple flavonoid compounds.
The tea plant ANS gene CsANS1 was cloned and identified, and its bifunctional catalytic activity was verified. The co-synthetic biosynthesis of anthocyanins and quercetin was achieved by constructing recombinant engineered bacteria. The anthocyanin synthase encoded by the CsANS1 gene was used to catalyze the conversion of colorless anthocyanins into anthocyanins and quercetin under α-ketoglutarate conditions.
This achievement enables the efficient and green synthesis of anthocyanins and quercetin, broadens the application prospects of ANS enzymes in metabolic engineering, and promotes the development and utilization of natural active ingredients in the food, pharmaceutical, and cosmetic fields.