Bacillus altitudinis BW65 and application thereof in disease resistance, yield increase and quality improvement

Abstract

The application belongs to the technical field of microorganisms, and particularly relates to a bacillus altitudinis BW65 strain against pear white line feather disease and application of the bacillus altitudinis BW65 strain in disease resistance, yield increase and quality improvement. Bacillus altitudinis The strain is isolated from the rhizosphere soil of a peach tree and is classified and named as the BW65 strain. The strain has the abilities of IAA production, nitrogen fixation, organic phosphorus and inorganic phosphorus decomposition, can significantly promote the growth of pear, tomato and other plants, improve the root morphology, increase the biomass and fruit quality, and has antagonistic effects on multiple pathogenic bacteria such as rice blast, alternaria alternata, fusarium graminearum and botryosphaeria dothidea. Application of the bacterial agent can improve the yield and quality of crops, reduce the use of chemical fertilizers and pesticides, and is suitable for the preparation of biological organic fertilizer and green agricultural production.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of microbial technology, in particular to a bacillus altitudinis strain BW65 against pear white strip and its application in disease resistance, yield increase and quality improvement. BACKGROUND

[0002] In modern agricultural production, plant growth promotion, fruit quality improvement and disease control are key links to improve the yield and quality of agricultural products. For a long time, people mainly rely on chemical fertilizers and pesticides to achieve these goals. Although these chemical means have significant effects in the short term, their long-term and large-scale use has led to a series of serious problems, including soil structure destruction, fertility decline, ecological environment pollution and pathogen resistance, which seriously restrict the sustainable development of agriculture.

[0003] In recent years, with the development of biotechnology, the green control technology of using plant rhizosphere beneficial microorganisms to promote growth, improve stress resistance and prevent soil-borne diseases has been increasingly valued. Among them, Bacillus microorganisms have become a research and application hotspot due to their strong stress resistance, fast reproduction, easy production and storage, etc. Bacillus altitudinis, as a kind of Bacillus, has been reported to have multiple biological activities and shows potential application value in plant growth promotion and disease biological control.

[0004] However, the current exploration of Bacillus altitudinis strain resources is still not sufficient, especially the systematic research and application report of specific strains with high-efficiency antagonism to diseases of important economic crops such as pear trees, and with the functions of significant growth promotion and fruit quality improvement is still scarce. The lack of effective microbial technology solutions that can comprehensively solve the problems of disease resistance, yield increase and quality improvement in the prior art limits the further development and application of Bacillus altitudinis in green agriculture. Therefore, it is of great significance to isolate and screen new Bacillus altitudinis strains with multiple excellent agronomic traits, and to develop their application technology, in order to reduce the use of chemical inputs and promote the sustainable development of agriculture. SUMMARY

[0005] The present application aims at the deficiencies of the prior art, and provides a new Bacillus altitudinis strain BW65 with multiple probiotic functions.

[0006] In order to achieve the above-mentioned purpose, the present application screens a Bacillus altitudinis strain BW65, which is classified and named as: Bacillus altitudinis BW65, and has a preservation number of CCTCC NO: M20251608; the strain is preserved in the China Center for Type Culture Collection, Wuhan University, Wuhan, China, and the preservation date is July 15, 2025.

[0007] The present application also provides a microbial agent containing the Bacillus altitudinis strain BW65.

[0008] The present application also provides an application of the Bacillus altitudinis strain BW65 or the microbial agent in preparing a bio-organic fertilizer.

[0009] The present application also provides an application of the Bacillus altitudinis strain BW65 or the microbial agent in promoting plant growth.

[0010] The present application also provides an application of the Bacillus altitudinis strain BW65 or the microbial agent in improving the quality of plant fruits.

[0011] Further, the plant is pear and / or tomato.

[0012] The present application also provides an application of the Bacillus altitudinis strain BW65 or the microbial agent in preparing a biocontrol preparation for preventing and controlling plant pathogens.

[0013] Further, the plant pathogen is Magnaporthe oryzae, Alternaria alternata, Fusarium graminearum and / or Botryosphaeria dothidea.

[0014] The application also provides application of the Bacillus altitudinis strain BW65 or the microbial inoculant in IAA production, nitrogen fixation, organic phosphorus and / or inorganic phosphorus decomposition.

[0015] The application also provides a method for promoting plant growth by using the Bacillus altitudinis strain BW65 or the microbial inoculant, which comprises applying a bacterial suspension of the Bacillus altitudinis strain BW65 to the rhizosphere of a plant seedling.

[0016] Further, the final concentration of the bacterial suspension is 10 7 CFU / g of soil.

[0017] In summary, the application has the following advantages due to the adoption of the above technical solutions.

[0018] The Bacillus altitudinis strain BW65 screened by the application has multiple growth promotion and pathogenic bacteria antagonistic functions. The strain can secrete IAA, fix nitrogen, and dissolve organic phosphorus and inorganic phosphorus, effectively promoting the absorption and utilization of nutrients by plants. Through potting and field tests, it is confirmed that BW65 can significantly improve the biomass, plant height, stem diameter and chlorophyll content of pear and tomato seedlings, improve root morphology, and increase root length, root surface area, root volume and root tip number. In addition, BW65 can significantly increase the single fruit weight, longitudinal diameter, transverse diameter, total sugar and soluble solid content of tomato fruits, reduce the titratable acid content, and improve the internal and external quality of the fruits. The plate confrontation test shows that BW65 has obvious antagonistic effect on multiple pathogenic bacteria such as rice blast, alternaria alternata, fusarium graminearum and botryosphaeria rhodina. Field application shows that BW65 can increase the yield per plant and single fruit weight of pear trees, reduce the rate of bad fruits, and reduce fruit rot. Therefore, the strain can be used as an important raw material for biological organic fertilizer and biological control agent, reducing the dependence on chemical fertilizers and pesticides, and promoting the sustainable development of green agriculture. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 It is a colony morphology diagram of the strain BW65.

[0020] Figure 2 It is a phylogenetic tree diagram of the strain BW65.

[0021] Figure 3 It is a growth promotion ability analysis result diagram of the strain BW65.

[0022] Figure 4 It is a phenotype result diagram of the strain BW65 on the growth of pear seedlings.

[0023] Figure 5 It is a phenotype result diagram of the strain BW65 on the root system of pear seedlings.

[0024] Figure 6 Figure 1. Phenotypic results of tomato seedling growth by strain BW65.

[0025] Figure 7 Figure 2. Phenotypic results of tomato seedling root system affected by strain BW65.

[0026] Figure 8 Figure 3. Results of the effect of strain BW65 on tomato fruit quality.

[0027] Figure 9 Figure 4. Results of the plate confrontation test of strain BW65 with various pathogenic bacteria.

[0028] Figure 10 Figure 5. Results of the effect of strain BW65 on pear fruit yield and quality in field application. DETAILED DESCRIPTION

[0029] All features disclosed in this specification, and / or all steps of any methods or processes disclosed in this specification, may be combined in any combination, except combinations where at least some of such features and / or steps are mutually exclusive.

[0030] Any feature disclosed in this specification, unless stated otherwise, may be replaced by any feature providing the same, or substantially the same, or equivalent functionality.

[0031] The culture media, reagents and instruments involved in the experiment can be purchased on the market.

[0032] Example 1.

[0033] This example is the screening, identification and biological characteristics of Bacillus altitudinis strain BW65.

[0034] 1. Isolation and purification of strain BW65: 1 g of rhizosphere soil was collected from the rhizosphere of peach trees in Yangshan Peach Orchard in Wuxi, added to a 50 ml flask containing glass beads and 9 ml of SM buffer, and 30°C, 170 shaking for 30 min to obtain a soil suspension. Sterile water was gradually diluted to 10 -5 ~10 -7 The concentration of the suspension was 10

[0035] 2. Identification of strain BW65: The morphological identification was performed as follows: BW65 strain was inoculated on TSA medium and placed in a constant temperature incubator for culture. After 24 h of culture, round colonies appeared on the culture dish, no spores, moist colonies, and milky white (as shown in Figure 1 ). The phylogenetic tree (as shown in Figure 2), we found that BW65 was consistent with the 16S sequence of non-model strain Bacillus altitudinis 41KF2b, in the same branch, and was Bacillus altitudinis.

[0036] The applicant has preserved the Bacillus altitudinis BW65 strain in the China Center for Type Culture Collection, with the preservation number CCTCC NO: M20251608; the preservation address is Wuhan, Wuhan University, China, and the preservation date is July 15, 2025.

[0037] Example 2.

[0038] This example is the study on the growth-promoting function of the Bacillus altitudinis BW65 strain.

[0039] 1. Qualitative determination of the IAA-producing function of the strain: add the BW65 strain to the TSB medium containing L-tryptophan at an inoculation amount of 1%, shake at 30°C and 170 r / min -1 After shaking for 48 h, centrifuge at 10,000 rpm for 5 min to obtain the supernatant, take 100 μl of the supernatant and mix with an equal volume of Salkowski colorimetric solution, mix 100 μl of uninfected medium with an equal volume of Salkowski colorimetric solution as a blank control, react for 30 min under dark conditions, and the solution turns red, indicating that the strain has the ability to produce IAA.

[0040] 2. Determination of the nitrogen fixation ability of the strain: add 10 μL of the activated BW65 bacterial suspension to the Arsenic nitrogen fixation medium, triplicate, cultivate in a 28°C incubator for 5 d, observe whether there is a halo or transparent circle on the medium, and judge the nitrogen fixation ability of the strain according to the ratio of the halo or transparent circle (D) to the colony diameter (d).

[0041] 3. Determination of the inorganic phosphorus solubilizing ability of the strain: add 10 μL of the activated BW65 bacterial suspension to the PKO inorganic phosphorus plate, triplicate, cultivate in a 28°C incubator for 5 d, observe whether there is a halo or transparent circle on the medium, and judge whether the strain has the ability to solubilize inorganic phosphorus according to the ratio of the halo or transparent circle (D) to the colony diameter (d).

[0042] 4. Determination of the organic phosphorus solubilizing ability of the strain: add 10 μL of the activated BW65 bacterial suspension to the Mengjina organic phosphorus plate, triplicate, cultivate in a 28°C incubator for 5 d, observe whether there is a halo or transparent circle on the medium, and judge whether the strain has the ability to solubilize inorganic phosphorus according to the ratio of the halo or transparent circle (D) to the colony diameter (d).

[0043] The above growth-promoting property determination results are all qualitative tests, and "+" is used to indicate "yes" and "-" is used to indicate "no", and the results are shown in Table 1.

[0044]

[0045] As shown in Table 1, the screened BW65 strain has the ability to produce IAA, fix nitrogen, and solubilize organic and inorganic phosphorus.

[0046] Example 3.

[0047] This example studies the growth-promoting effect of Bacillus hygroscopicus BW65 inoculant on pear seedlings.

[0048] (1) Pear seedling cultivation: Soak pear seeds in sterile water for 12 hours, then add 1% hydrogen peroxide for 20 minutes for disinfection, and finally rinse with sterile water. Spread the disinfected seeds evenly on a culture medium with sterile water-soaked filter paper, and place them in a 28℃ constant temperature incubator for 3-5 days to germinate. Select pear seedlings with white sprouts and sow them in sterilized seedling substrate. When the pear seedlings have 2-3 true leaves, select healthy and uniform seedlings and transplant them into sterilized cultivation substrate flower pots.

[0049] (2) Preparation of BW65 bacterial suspension: The strain BW65, preserved in glycerol tubes at -80℃, was purified by streaking on TSA medium. After culturing at 28℃ for 12 h, a single colony was picked and transferred to TSB liquid medium and cultured overnight at 28℃ and 170 r·min⁻¹ to activate the strain. Then, the seed culture was inoculated into fresh TSB liquid medium at an inoculation rate of 1% (volume fraction) and cultured at 28℃ and 170 r·min⁻¹ for 12 h with shaking to obtain the BW65 fermentation broth. The OD600 was adjusted to 1.0, and the cells were collected after centrifugation. The cells were resuspended three times and diluted with sterile water to a viable cell concentration of 10⁷ CFU / mL for use.

[0050] (3) Inoculation: Inoculate the prepared BW65 bacterial suspension (final concentration 10) 7 CFU / g soil was inoculated into the rhizosphere of *Pyrus pyrifolia* seedlings, with the uninoculated solution serving as a control (CK). Each treatment was replicated 6 times, and samples were collected after 30 days of culture to determine the phenotypic traits of *Pyrus pyrifolia*.

[0051] Experimental results.

[0052] 1. For example Figure 3 As shown, BW65 inoculant significantly promoted the growth of pear seedlings. Compared with the control (CK), the aboveground dry weight, root dry weight, and total dry weight of pear seedlings treated with BW65 increased by 35.7%, 3.6%, and 26.3%, respectively. The plant height, stem diameter, and SPAD of pear seedlings treated with BW65 were significantly increased by 62.7%, 17.9%, and 12.9%, respectively, compared with the CK.

[0053] 2. For example Figure 4As shown, the BW65 bacterial agent can improve the root morphology of pear seedlings, and the root surface area, root length, root volume and root tip number are increased by 8.4%, 7.6%, 7.8% and 9.3%, respectively.

[0054] Example 4.

[0055] This example is a study on the growth-promoting effect of Bacillus altitudinis BW65 bacterial agent on tomato seedlings.

[0056] (1) Tomato seedling raising: Seed treatment: Take healthy tomato seeds, first soak in sterile water for 24 hours; then soak in 75% alcohol for 30 seconds, then rinse 3-5 times with sterile water; then, soak in 2% sodium hypochlorite solution for 20 minutes, and finally rinse thoroughly with a large amount of sterile water.

[0057] Germination and sowing: After disinfection, the seeds are placed on the culture medium with sterile water soaked filter paper, and placed in a 28°C constant temperature incubator for 3-5 days. Select seeds with good germination and sow them in sterile culture medium or sterile substrate for seedling raising.

[0058] Transplanting: After the tomato seedlings grow to 2-3 true leaves and have uniform growth, select healthy seedlings and transplant them into sterile substrate pots for pot culture.

[0059] (2) Bacterial inoculation: Prepare bacterial suspension: according to the method described above, prepare Bacillus altitudinis BW65 bacterial suspension, adjust the final concentration to 10 7 CFU / g soil.

[0060] CK group (control group): No bacteria inoculation, only tomato seedlings are cultivated.

[0061] BW65 group: Only Bacillus altitudinis BW65 bacterial suspension is inoculated, with a concentration of 10 7 CFU / g soil.

[0062] Bacterial inoculation: The prepared bacterial suspension is evenly inoculated on the rhizosphere of tomato seedlings.

[0063] Experimental design: Each treatment group has 6 replicates, and the culture is carried out under the same environmental conditions.

[0064] Result collection: After 30 days of culture, the growth phenotype of tomato seedlings in each group is determined and recorded, including plant height, root development, biomass and other indicators.

[0065] Test results.

[0066] 1. As Figure 5As shown, inoculation with BW65 inoculant significantly promoted the growth of tomato seedlings. Compared with the control (CK), the aboveground fresh weight, root fresh weight, and total fresh weight of tomato seedlings treated with BW65 inoculant suspension increased by 8.5%, 192%, and 16.6%, respectively, while the stem diameter and plant height increased by 3.5% and 16.3%, respectively, compared with the CK.

[0067] 2. For example Figure 6 As shown, inoculation with BW65 bacterial agent can significantly improve the root morphology of tomatoes. Compared with the control CK, the root length, root surface area, number of root tips and root volume of tomatoes treated with BW65 bacterial suspension increased by 61.7%, 29.7%, 16.8% and 5.0%, respectively.

[0068] Example 5.

[0069] This example illustrates the effect of Bacillus hygroscopicus BW65 inoculum on the quality of tomato fruits.

[0070] (1) Tomato seedling cultivation: Seed treatment: Take healthy tomato seeds and soak them in sterile water for 24 hours; then soak them in 75% alcohol for 30 seconds, and then rinse them with sterile water 3-5 times; then soak them in 2% sodium hypochlorite solution for 20 minutes, and finally rinse them thoroughly with plenty of sterile water.

[0071] Germination and sowing: Spread the sterilized seeds evenly on a culture medium lined with sterile, water-moistened filter paper, and place them in a 28℃ constant temperature incubator for 3-5 days to germinate. Select seeds with good germination and sow them in sterile culture medium or sterile substrate for seedling cultivation.

[0072] Transplanting: Once the tomato seedlings have grown to 2-3 true leaves and are growing evenly, select healthy and uniform seedlings and transplant them into sterilized substrate pots for pot cultivation.

[0073] (2) Inoculation treatment: Preparation of bacterial suspension: According to the above method, prepare a bacterial suspension of Bacillus altitudinis BW65 and adjust its final concentration to 10. 7 CFU / g soil.

[0074] CK group (control group): Tomato seedlings were cultivated alone without any bacterial inoculation.

[0075] Group BW65: Inoculated only with a suspension of Bacillus altitudinis BW65 bacteria at a concentration of 10. 7 CFU / g soil.

[0076] Inoculation procedure: The prepared bacterial suspension is evenly inoculated into the root zone of tomato seedlings.

[0077] Experimental design: 6 replicates were set for each treatment group, and the culture was carried out under the same environmental conditions. Then the fruit quality and other related indicators were determined.

[0078] Test results.

[0079] 1. As shown in Figure 7 , inoculation of BW65 bacterial suspension can significantly improve the appearance quality of tomato fruits. Compared with the control CK, the longitudinal diameter, transverse diameter and single fruit weight of tomato fruits treated with BW65 bacterial suspension were increased by 13.5%, 5.9% and 81%, respectively.

[0080] 2. As shown in Figure 8 , inoculation of BW65 bacterial suspension can significantly improve the internal quality of tomato fruits. Compared with the control CK, the water content, total sugar content and soluble solid content of tomato fruits treated with BW65 bacterial suspension were increased by 0.9%, 12.3% and 16.7%, respectively; the titratable acid in tomato fruits treated with BW65 bacterial suspension was significantly reduced by 12.2% compared with CK treatment.

[0081] Example 6.

[0082] This example is the application effect research of Bacillus altitudinis BW65 strain in the field.

[0083] To explore the effect of BW65 bacterial agent on fruit quality, 10 fruit trees of the same age were selected as test objects and randomly divided into 2 groups, 5 trees in each group.

[0084] Inoculation treatment: Prepare bacterial suspension: according to the method described above, prepare Bacillus altitudinis BW65 into bacterial suspension, adjust its final concentration to 10 7 CFU / g soil.

[0085] CK group (control group): 2 inoculation points were set for each tree, 1L of distilled water was added to each pit, and a total of 3 times of distilled water was added.

[0086] BW65 group: 2 inoculation points were set for each tree, 1L of Bacillus altitudinis BW65 bacterial suspension was added to each pit, with a concentration of 10 7 CFU / g soil, and inoculation was performed 3 times on June 10 (young fruit stage), July 9 and July 21 (enlargement stage) respectively.

[0087] Other management schemes are the same.

[0088] Result collection: 30 days after the last inoculation, the fruit trees of each group were harvested, and the fruit rot incidence, fruit transverse diameter and longitudinal diameter, fruit soluble solid content and acidity were measured and recorded.

[0089] The results of the measurements of fruit yield per plant, number of fruits, and weight per fruit are shown in Table 2.

[0090]

[0091] Note: Different letter markings indicate significant differences.

[0092] Based on the test results Figure 10 As you can see, the CK (control) fruit had a large number of rotten fruits, and the severity of the damage was significant. Inoculation with BW65 improved this situation. Field results showed that the transverse and longitudinal diameters of fruits inoculated with BW65 were significantly increased compared to CK, and the weight of individual fruits increased (average 260.8g), an increase of 12.4%, thus improving marketability. The average yield per tree treated with BW65 was 22.3kg, which was higher than CK. Simultaneously, the incidence of rotten fruit was significantly reduced with BW65, decreasing by 43.9% compared to CK, effectively addressing fruit rot in the field and demonstrating a certain degree of disease resistance. Furthermore, BW65 increased the soluble solids content of the fruit, while reducing acidity by 23.5% compared to CK.

[0093] Example 7.

[0094] This example demonstrates a plate confrontation experiment between Bacillus hygroscopicus strain BW65 and various pathogens.

[0095] Multiple pathogens were cultured on PDA medium. Then, the activated fungal strains were prepared into 5mm mycelial discs and transferred to the center of a new PDA plate. 1μl of OD200 was then spotted around the fungal discs. 600 The bacterial suspension was prepared at 0.80. After the suspension dried, the plates were placed in an incubator at 28°C for incubation. Sterile water was spotted around the perimeter as a control group. Each treatment was performed in triplicate. The plate phenomena were observed and photographed after 5 days of incubation.

[0096] Result: As Figure 9 As shown, strain BW65 can significantly antagonize Magnaphthora oryzae, Alternaria alternata, Fusarium graminearum, and Pterocarya spp. (white-striped feather), indicating that strain BW65 has a certain degree of disease resistance.

[0097] In summary, the test proves that the high-altitude Bacillus strain BW65 screened by the applicant has good abilities of IAA production, nitrogen fixation, organic phosphorus degradation, and inorganic phosphorus degradation, and can significantly promote the growth of pear and tomato seedlings, improve root morphology, and increase plant biomass. Specifically, compared with the control, the aboveground dry weight, root dry weight, and total dry weight of pear seedlings treated with BW65 inoculant increased by 35.7%, 3.6%, and 26.3%, respectively, and the plant height, stem diameter, and SPAD value increased by 62.7%, 17.9%, and 12.9%, respectively; the aboveground fresh weight, root fresh weight, and total fresh weight of tomato seedlings increased by 8.5%, 192%, and 16.6%, respectively, and the stem diameter and plant height increased by 3.5% and 16.3%, respectively. In addition, the strain BW65 has obvious antagonistic effect on pathogenic fungi such as Magnaporthe oryzae, Alternaria alternata, Fusarium graminearum, and Ceratocystis subrufa (white streak). Pot experiment shows that inoculation of BW65 bacterial suspension can significantly improve the aboveground dry weight, root dry weight, plant height, stem diameter, and other growth indicators of pear and tomato, and can improve the appearance and internal quality of tomato fruits. The fruit longitudinal diameter, transverse diameter, single fruit weight, total sugar content, and soluble solid content are significantly improved, and the acidity is reduced. Field application effect research shows that the average single plant yield and average single fruit weight of fruit trees inoculated with BW65 are significantly improved, the incidence of bad fruit is significantly reduced, the soluble solid content of fruit is increased, and the acidity is reduced. The strain can be used to prepare biological inoculant or biological organic fertilizer, reduce the use of chemical fertilizer, and promote the development of green agriculture.

[0098] Although the present application has been described in detail above with general description and specific embodiments, some modifications or improvements can be made on the basis of the present application, which is obvious to those skilled in the art. Therefore, these modifications or improvements made on the basis of not deviating from the spirit of the present application, all belong to the scope of the present application claimed.

Claims

1. Highland Bacillus ( Altitude Bacillus ) strain BW65 or containing Bacillus hygroscopicus ( Bacillus height The application of bacterial agent of strain BW65 in the preparation of biocontrol agents for controlling plant pathogens, wherein the plant pathogen is *Magnaporthe oryzae* (rice blast fungus). Magnaporthe rice Alternaria ( Alternaria alternata Fusarium graminearum ( ), Fusarium graminearum ( Fusarium gramineae ) and brown spores ( Rosellinia necatrix The Highland Bacillus ( Altitude Bacillus strain BW65, its classification name is: Altitude Bacillus BW65, with accession number CCTCC NO: M20251608, is deposited at the China Center for Type Culture Collection, Wuhan University, Wuhan, China, on July 15, 2025.

2. The Bacillus hygroscopicus as described in claim 1 ( Altitude Bacillus ) strain BW65 or containing Bacillus hygroscopicus ( Altitude Bacillus The application of strain BW65 in the preparation of bio-organic fertilizer, wherein the organism is pear and / or tomato.

3. The Bacillus hygroscopicus as described in claim 1 ( Altitude Bacillus ) strain BW65 or containing Bacillus hygroscopicus ( Altitude Bacillus The application of strain BW65 in the promotion of plant growth, wherein the plant is pear and / or tomato.

4. The Bacillus hygroscopicus as described in claim 1 ( Altitude Bacillus ) strain BW65 or containing Bacillus hygroscopicus ( Altitude Bacillus The application of Bacillus strain BW65 in improving the quality of plant fruits, wherein the plant is pear and / or tomato; the improvement of plant fruit quality is that Bacillus strain BW65 can significantly increase the single fruit weight, longitudinal diameter, transverse diameter, total sugar and soluble solids content and reduce the titratable acid content of tomato fruits; the improvement of plant fruit quality is that Bacillus strain BW65 can increase the yield per pear tree, single fruit weight and soluble solids content.

5. An application of the *Bacillus hygroscopicus* as described in claim 1 (… Altitude Bacillus ) strain BW65 or containing Bacillus hygroscopicus ( Altitude Bacillus A method for promoting plant growth using a bacterial agent of strain BW65, characterized in that, The method is as follows: Bacillus hygroscopicus (Bacillus hygroscopicus) Altitude Bacillus The bacterial suspension of strain BW65 and / or bacterial agent were applied to the rhizosphere of the plant seedlings; the plant was pear and / or tomato.

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