Preparation method of dendrobium huoshanense oligosaccharide with hepatoprotective activity

By controlling extraction and enzymatic hydrolysis parameters and combining ultrafiltration and centrifugation, oligosaccharides from Dendrobium huoshanense with significant hepatoprotective activity were prepared, solving the problem of insignificant efficacy of existing drugs and achieving a highly effective and low-toxicity hepatoprotective effect.

CN121160813BActive Publication Date: 2026-04-21ANHUI DENDROBIUM BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202511318390.1
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-09-16
Publication Date
2026-04-21
Estimated Expiration
2045-09-16

AI Technical Summary

Technical Problem

Existing drugs are not very effective and have significant side effects in treating liver diseases such as fatty liver and liver fibrosis. Research on the hepatoprotective activity of Dendrobium huoshanense oligosaccharides is not yet in-depth, which limits the comprehensive understanding of its pharmacological effects.

Method used

The crude polysaccharides of Dendrobium huoshanense were extracted and refined by controlling the temperature, time, and material-liquid ratio. Specific β-mannanase was used for enzymatic hydrolysis, combined with molecular weight cutoff membrane ultrafiltration and centrifugation to prepare Dendrobium huoshanense oligosaccharides with a molecular weight ≤3000 Da. Finally, the oligosaccharides were concentrated and freeze-dried.

Benefits of technology

The prepared Dendrobium huoshanense oligosaccharides showed significant hepatoprotective activity in an acetaminophen-induced HepG2 cell injury model, with a low IC50 value, demonstrating promising application prospects.

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Abstract

The present application relates to the technical fields of oligosaccharide preparation, and discloses a preparation method of dendrobium huoshanense oligosaccharide with liver protection activity, and the specific steps are as follows: first, fresh dendrobium huoshanense stems are selected, impurities are removed, and low-temperature drying is performed, then deionized water is added and heated to extract dendrobium crude polysaccharide; then the crude polysaccharide solution is refined to obtain dendrobium refined polysaccharide; then beta-mannanase is added to the dendrobium refined polysaccharide extraction solution for enzymolysis, the polysaccharide solution is removed through a molecular cut-off membrane, and dendrobium huoshanense oligosaccharide stock solution concentrate is obtained; finally, the finished product is obtained through freeze-drying. The preparation method is simple, efficient and pollution-free, and the prepared dendrobium huoshanense oligosaccharide has significant effects in liver protection.
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