InDel molecular marker sl-12 for identifying peanut leaf size, primer pair and application thereof

By developing the InDel molecular marker SL-12 and its primer pair, the precise localization and early selection of peanut leaf size genes were achieved, solving the problems of high difficulty in developing molecular markers and long breeding cycles in peanut breeding, and realizing an efficient and low-cost breeding method.

CN121183030BActive Publication Date: 2026-05-15SHANDONG ACADEMY OF AGRICULTURAL SCIENCES
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Patent Information

Application Number
CN202511737777.0
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-11-25
Publication Date
2026-05-15
Estimated Expiration
2045-11-25

AI Technical Summary

Technical Problem

Developing molecular markers for peanut leaf size-related genes is challenging. Traditional breeding methods rely on long-term field phenotypic identification, which is also affected by the environment, making it difficult to achieve early selection and efficient breeding.

Method used

Develop an InDel molecular marker SL-12 and its primer pair for PCR amplification and gel electrophoresis detection to accurately locate the peanut leaf size gene. Provide kits and identification methods to enable genotyping and early selection in the seedling stage.

Benefits of technology

By using molecular marker-assisted breeding, the breeding cycle can be shortened, the accuracy and efficiency of selection can be improved, and the cost can be reduced, thus enabling the rapid breeding of new small-leaf peanut varieties and improving photosynthetic efficiency.

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Abstract

The application belongs to the technical field of molecular biology, and relates to an InDel molecular marker SL-12 for identifying peanut leaf size, a primer pair and application of the InDel molecular marker SL-12. The InDel molecular marker SL-12 has a nucleotide sequence as shown in SEQ ID NO:1. A 459 bp fragment can be specifically amplified in small leaf material by using the primer pair for PCR amplification. The InDel marker SL-12 of the peanut leaf size related gene is disclosed for the first time, DNA of an F2 separation population generated after hybridization of small leaves and normal leaves is subjected to PCR amplification, and gel electrophoresis detection can effectively distinguish small leaf materials, and the InDel marker SL-12 can be used for small leaf peanut new variety breeding. The technology is accurate and reliable, stable, fast and low in detection cost.
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