Application of Brassica napus BnaALaAT1 gene in breeding plant varieties with stress resistance and high yield

By genetic transformation and expression vector construction of the rapeseed BnaALaAT1 gene, the trade-off between stress resistance and yield in rapeseed breeding was solved, achieving high and stable yields under adverse conditions and improving the stress resistance and yield of rapeseed.

CN121249777BActive Publication Date: 2026-03-27OIL CROPS RES INST CHINESE ACAD OF AGRI SCI
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Patent Information

Application Number
CN202511821447.X
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-12-05
Publication Date
2026-03-27
Estimated Expiration
2045-12-05

AI Technical Summary

Technical Problem

In existing rapeseed breeding, there is a trade-off between stress resistance and yield. Traditional methods are difficult to maintain high and stable yields under adverse conditions, and the screened stress resistance genes lose their function under combined field stress, affecting the stability of rapeseed production.

Method used

Genetic transformation of the rapeseed BnaALaAT1 gene was used to construct an overexpression vector. Pure lines of the versatile gene were obtained through Agrobacterium-mediated transformation. This gene regulates multiple stress response mechanisms in plants, enhances their survival ability, and achieves synergistic optimization of stress resistance and high yield by optimizing carbon and nitrogen metabolism and coordinating resource allocation.

Benefits of technology

It significantly improved the growth status of transgenic plants under adverse conditions, increasing yield by 53.7% and oil content by 23%, breaking the bottleneck in the trade-off between stress resistance and yield, achieving high and stable yields under adverse conditions, and providing an efficient genetic solution.

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Abstract

This invention discloses a rapeseed BnaALaAT1 The application of genes in breeding stress-resistant, high-yielding plant varieties, the aforementioned BnaALaAT1 The nucleotide sequence of the gene is shown in SEQ ID NO.1. This invention clones the versatile gene from rapeseed. BnaALaAT1 We constructed pure-line plants by introducing rapeseed and conducted phenotypic investigations under various conditions. The experimental results showed that... BnaALaAT1 Genetically modified rapeseed exhibits significantly superior growth compared to the wild type under both normal and various adverse conditions, fully demonstrating that this gene can enhance the plant's survival ability under various adverse conditions by regulating the plant's response mechanism to multiple stresses, providing a crucial guarantee for the stable growth of plants under diverse adverse conditions. Compared to the wild type, transgenic plants not only show a significant increase in yield, with a maximum increase of 53.7%, but also a significant increase in oil content.
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