KASP molecular marker related to sheep milk fat percentage character, detection primer group and application of KASP molecular marker

By designing a specific KASP primer set to detect the C>T variant at the sheep Chr15:g.12674721 site, the problem of inefficient breeding of sheep milk fat percentage trait was solved, enabling early and accurate molecular marker-assisted selection, thereby improving breeding efficiency and sheep milk product quality.

CN121320570APending Publication Date: 2026-01-13TIANJIN ACAD OF AGRI SCI
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Patent Information

Application Number
CN202511815062.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-12-04
Publication Date
2026-01-13

AI Technical Summary

Technical Problem

The milk fat percentage trait in sheep is regulated by multiple genes, making it difficult to achieve efficient molecular marker-assisted selection through traditional methods, resulting in low breeding efficiency.

Method used

A specific KASP primer set was designed to detect the C>T variant at the Chr15:g.12674721 locus in sheep. Combined with KASP genotyping PCR amplification and fluorescence signal analysis, early and accurate identification and molecular marker-assisted selection of sheep milk fat percentage traits were achieved.

Benefits of technology

This has enabled efficient and precise breeding of milk fat percentage traits, shortened the generation interval of breeding, increased selection intensity, and improved the nutritional value of goat milk products and the health of sheep flocks.

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Abstract

The invention relates to the technical field of molecular detection, and aims to provide a method for detecting sheep Chr15: g.12674721 site Cgt; the primer group is composed of the following three primers: a site C allele typing primer FAM, the nucleotide sequence of which is shown as SEQ ID NO: 1, a site C allele typing primer FAM, a site C allele typing primer FAM, a site C allele typing primer FAM, a site C allele typing primer FAM and a site C allele typing primer FAM, the nucleotide sequence of the locus T allele typing primer VIC is as shown in SEQ ID NO: 2; and the nucleotide sequence of the universal downstream primer is as shown in SEQ ID NO: 3. Wherein the Chr15: g.12674721 locus is based on a sheep reference genome version AR-UIRamv3.0, and the recombinant plasmid can be used for preparing a recombinant plasmid. The invention discovers and discloses a Cgt of a Chr15: g.12674721 site in a sheep genome for the first time; the T single nucleotide polymorphism (SNP) is obviously associated with the milk fat percentage character. Association analysis of 140 Hu sheep proves that the cream-fat percentage of a CC genotype individual is obviously higher than that of a CT type individual and a TT type individual. The discovery provides a direct and reliable molecular target for genetic improvement of the fat percentage of sheep.
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