A strain of bresadia zonata and application thereof in repairing and reducing ginseng continuous cropping obstacles

Abstract

This invention discloses a garlic-flavored *Pteris vittata* strain and its application in ginseng-inspired crop rotation restoration and reduction of continuous cropping obstacles in *Gastrodia elata*, belonging to the field of agricultural biotechnology. The *Pteris vittata* strain MS-GY-GLY, with accession number CGMCC No. 42371, was isolated from ginseng rotation soil. This strain possesses multiple functions, including inhibiting pathogens, degrading allelopathic substances, and promoting *Gastrodia elata* growth, achieving microbial restoration of continuous cropping obstacles. Applying this strain to the *Gastrodia elata* cultivation system not only significantly reduces the content of various allelopathic substances in the soil and improves soil physicochemical properties, but also effectively suppresses soil pests, creating a favorable soil environment for *Gastrodia elata* growth. This invention combines this strain with Sichuan pepper extract and a specific cultivation substrate to construct a comprehensive technical system that can solve continuous cropping obstacles in *Gastrodia elata*, increase yield and efficacy, and provide technical support for the sustainable development of the *Gastrodia elata* industry.

Description

Technical Field

[0001] This invention relates to the field of agricultural biotechnology, and in particular to a garlic-flavored *Micrococcus pumila* strain and its application in ginseng-inspired crop rotation repair and reduction of continuous cropping obstacles in *Gastrodia elata*. Background Technology

[0002] Gastrodia elata Bl. possesses various medicinal properties, including calming the liver and extinguishing wind, invigorating qi and relieving convulsions, promoting blood circulation and regulating qi, and relieving pain. It is a valuable traditional Chinese medicine, and its artificial cultivation area continues to increase with expanding market demand. However, due to limited suitable land resources, continuous cropping of Gastrodia elata is becoming increasingly common, highlighting the problem of continuous cropping obstacles. These obstacles mainly manifest as deterioration of soil physical and chemical properties, accumulation of allelopathic substances, and imbalance in soil microbial community structure, ultimately leading to increased disease severity and reduced yield, severely restricting the sustainable development of the industry. Currently, crop rotation, intercropping, soil disinfection, and the application of organic fertilizers are commonly used to alleviate continuous cropping obstacles. Among these, the rotation model between Gastrodia elata and winter fungus has achieved good results in some areas, providing a feasible path to solve the problem of continuous cropping of Gastrodia elata.

[0003] However, existing crop rotation techniques for Gastrodia elata have significant regional limitations. While *Fructus medica* is a commonly used rotation plant, its cold intolerance prevents its cultivation in Northeast China, hindering the widespread application of this model in the main production areas of *Gastrodia elata* in the Changbai Mountains. Furthermore, the selection of suitable plant species for rotation with Gastrodia elata is still incomplete, particularly regarding a rotation plant system tailored to the cold climate of Northeast China. Current research has not elucidated the key mechanisms by which rotation plants affect the transformation of allelochemicals, the improvement of physicochemical properties, and the reconstruction of microbial communities in soils continuously cropped with Gastrodia elata, resulting in a lack of theoretical guidance and precise control basis for crop rotation techniques.

[0004] A more prominent problem is that current research on the core causes and restoration mechanisms of continuous cropping obstacles in Gastrodia elata still has many gaps. The types of specific pathogens in continuously cropped soils and their pathogenic mechanisms are not yet clear, and corresponding antagonistic bacterial resources urgently need to be explored; microorganisms that degrade soil allelopathic substances have not been systematically screened; and there are no reports on the development of specialized improvement substrates and plant-derived biological pesticides for continuous cropping obstacles. The lack of these key technologies makes it difficult for existing crop rotation models to fundamentally restore continuous cropping obstacles, thus hindering the healthy development of the Gastrodia elata industry. Therefore, it is urgent to establish a new Gastrodia elata crop rotation method suitable for Northeast China, capable of synergistically improving soil microecology and efficiently reducing continuous cropping obstacles. Summary of the Invention

[0005] The purpose of this invention is to provide a *Symplocos garicus* strain and its application in ginseng-inspired crop rotation restoration and reducing continuous cropping obstacles in *Gastrodia elata*, thereby addressing the problems existing in the prior art. This invention isolates a *Symplocos garicus* strain MS-GY-GLY from the rhizosphere soil of rotated ginseng, and combines it with Sichuan pepper extract and a specific cultivation substrate to construct a comprehensive technical system. This system can inhibit pathogens and pests, degrade allelopathic substances, improve soil, solve continuous cropping obstacles in *Gastrodia elata*, increase yield and efficacy, and provide technical support for the sustainable development of the industry.

[0006] To achieve the above objectives, the present invention provides the following solution:

[0007] This invention provides a garlic-flavored small umbrella, which is classified as garlic-flavored small umbrella (Mycetinis scorodonius) and was deposited at the China General Microbiological Culture Collection Center on November 17, 2025, with the accession number CGMCC No. 42371.

[0008] The present invention also provides a microbial inoculant comprising the aforementioned garlic-flavored small umbrella.

[0009] The present invention also provides an application of the garlic-flavored small umbrella or the microbial agent, the application including any one of the following (1)-(4):

[0010] (1) Inhibits the growth of pathogenic bacteria causing Gastrodia elata;

[0011] (2) Degrading allelochemicals in soil where Gastrodia elata is continuously cropped;

[0012] (3) Promote the growth of Gastrodia elata or increase the content of medicinal components in Gastrodia elata;

[0013] (4) Prepare products for repairing and reducing the obstacles of continuous cropping of Gastrodia elata.

[0014] Furthermore, the pathogenic fungi include Fusarium oxysporum, Fusarium aromaticum, Fusarium graminearum, Trichoderma harzianum, Trichoderma hookeriana, and Trichoderma tarda.

[0015] Furthermore, the allelochemicals include p-hydroxybenzyl alcohol, dibutyl phthalate, and vanillin.

[0016] Furthermore, the active ingredients of Gastrodia elata include gastrodin, p-hydroxybenzyl alcohol, barisonoside E, barisonoside B, and barisonoside C.

[0017] The present invention also provides a method for repairing and reducing the obstacles of continuous cropping of Gastrodia elata by imitating ginseng crop rotation, including the step of applying the garlic-flavored small umbrella or the microbial agent to the Gastrodia elata cultivation system.

[0018] Furthermore, it also includes the step of applying a 0.25% (w / v) concentration of Sichuan pepper ethanol extract to the Gastrodia elata cultivation system.

[0019] Furthermore, the preparation method of the Sichuan pepper ethanol extract includes ultrasonic extraction of Sichuan pepper powder with 95% ethanol at a material-to-liquid ratio of 1g:10mL, followed by filtration and concentration to obtain the Sichuan pepper ethanol extract.

[0020] Furthermore, the cultivation substrate of the Gastrodia elata cultivation system is composed of sand and sawdust in a volume ratio of 3:1, or of 100% fermented rice husks.

[0021] The present invention discloses the following technical effects:

[0022] This invention relates to a *Gastrodia elata* strain isolated from ginseng rotation soil. This strain possesses multiple functions, including inhibiting pathogens, degrading allelopathic substances, and promoting the growth of *Gastrodia elata*, thus achieving microbial remediation of continuous cropping obstacles. Applying this strain to the *Gastrodia elata* cultivation system not only significantly reduces the content of various allelopathic substances in the soil and improves soil physicochemical properties, but also effectively suppresses soil pests, creating a favorable soil environment for *Gastrodia elata* growth.

[0023] Furthermore, the Sichuan pepper extract screened in this invention, as a plant-derived pesticide, exhibits significant repellent effects against pests affecting Gastrodia elata, and can also increase the yield and efficacy of Gastrodia elata. When used in conjunction with a specific cultivation substrate, it forms a comprehensive technical system integrating crop rotation, biocontrol, allelopathic substance degradation, and soil improvement. The synergistic effect of the components in this system fundamentally solves the problem of continuous cropping obstacles for Gastrodia elata, providing reliable technical support for the sustainable development of the Gastrodia elata industry. Attached Figure Description

[0024] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0025] Figure 1 The effect of rotating ginseng, atractylodes and epimedium on the content of allelochemicals in soil from continuous cropping of Gastrodia elata on the contents of hydroxybenzyl alcohol (a), dibutyl phthalate (b) and vanillin (c);

[0026] Figure 2 The diagram shows the effects of crop rotation of ginseng, atractylodes and epimedium on soil physicochemical indicators such as available nitrogen (a), available phosphorus (b), available potassium (c), soil pH (d), and organic matter (e) in continuous cropping of gastrodia elata.

[0027] Figure 3The diagram shows the effect of crop rotation of ginseng, atractylodes and epimedium on the number of soil pests in continuous cropping of gastrodia; where (a) represents the number of nematodes and (b) represents the number of mites.

[0028] Figure 4 This is a colony morphology diagram of the garlic-flavored small cap mushroom;

[0029] Figure 5 The colony morphology of six pathogenic fungi in the soil of Gastrodia elata continuous cropping is shown in the figure. Among them, (a) is Fusarium oxysporum; (b) Fusarium aromaticum; (c) Fusarium graminearum; (d) Trichoderma harzianum; (e) Trichoderma hookerianum; and (f) Trichoderma pallida.

[0030] Figure 6 Phenotypic images of six pathogenic fungi from soil continuously cropped with Gastrodia elata on Gastrodia elata tubers; among them, (a) is Fusarium oxysporum, (b) Fusarium aromaticum, (c) Trichoderma hookerianum, (d) Trichoderma harzianum, (e) Fusarium graminearum, and (f) Trichoderma pallida.

[0031] Figure 7 The graph shows the inhibition rate of *Trichoderma harzianum* against the pathogen of *Gastrodia elata*. In the graph, JD represents *Fusarium oxysporum*, FX represents *Fusarium aromaticum*, HG represents *Fusarium graminearum*, HC represents *Trichoderma harzianum*, GZ represents *Trichoderma hookeriana*, and TG represents *Trichoderma tarda*.

[0032] Figure 8 The following is a statistical chart for screening plant-derived biological pesticides that are resistant to Gastrodia elata pests; (a) represents nematode mortality rate; (b) represents nematode attraction activity; (c) represents mite mortality rate; and (d) represents mite attraction activity. In the chart, LJ represents chili pepper, HJ represents Sichuan pepper, YY represents tobacco, JZ represents pyrethroid, BH represents mint, DS represents garlic, and AC represents artemisia.

[0033] Figure 9 A statistical chart showing the impact of plant-derived biopesticides on the number of pests in continuous cropping of Gastrodia elata; where (a) represents the number of nematodes and (b) represents the number of mites. Detailed Implementation

[0034] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0035] It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the invention. Furthermore, with respect to numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Any stated value or intermediate value within a stated range, as well as each smaller range between any other stated value or intermediate value within said range, is also included in this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0036] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.

[0037] Various modifications and variations can be made to the specific embodiments described in this specification without departing from the scope or spirit of the invention, as will be apparent to those skilled in the art. Other embodiments derived from this specification will also be apparent to those skilled in the art. This specification and embodiments are merely exemplary.

[0038] The terms “include,” “including,” “have,” “contain,” etc., used in this article are all open-ended terms, meaning that they include but are not limited to.

[0039] Example 1: Verification of the effect of ginseng rotation on the remediation of soil with continuous cropping obstacles caused by Gastrodia elata.

[0040] This embodiment aims to verify the effect of ginseng as a rotational plant on the remediation of soil continuously cropped with Gastrodia elata.

[0041] 1. Overview and Design of the Experimental Site

[0042] The experimental site was located in Dabeishan Village, Longquan Town, Jingyu County, Jilin Province (geographical coordinates: 126°30'–127°16' E, 42°06'–42°48' N). The site was a continuous cropping obstacle soil where Gastrodia elata had been cultivated for 5 consecutive years.

[0043] Four treatment groups were set up: ginseng rotation group (RS, ginseng rotation in old Gastrodia elata fields), Atractylodes lancea rotation soil (CZ), Epimedium rotation soil (YYH), and Gastrodia elata continuous cropping control group (TM, continuous cropping of Gastrodia elata). In addition, continuous cropping obstacle soil without any medicinal plant cultivation was used as a blank control (CK). Each treatment was replicated at least 3 times.

[0044] 2. Soil sample collection and analysis

[0045] After the crop rotation cycle ended, topsoil (0-20 cm) from each treatment group was collected using a five-point sampling method. The soil was mixed and passed through a 2 mm sieve for subsequent determination of allelochemicals, physicochemical properties, and pest numbers.

[0046] 3. Measurement methods and results

[0047] (1) Determination of allelochemical content: The contents of p-hydroxybenzyl alcohol, dibutyl phthalate, and vanillin in the soil were determined by ultraviolet spectrophotometry. The results are as follows: Figure 1 As shown, the contents of the three allelochemicals in ginseng-rotated soils were significantly lower than those in soils continuously cropped with Gastrodia elata (P < 0.05). The contents of p-hydroxybenzyl alcohol, vanillin, and dibutyl phthalate in ginseng-rotated soils decreased by approximately 30.8%, 11.3%, and 29.2%, respectively. Compared with Atractylodes lancea and Epimedium, ginseng rotation was more effective in mitigating the allelopathic stress of Gastrodia elata in soils continuously cropped with Gastrodia elata.

[0048] (2) Determination of soil physicochemical properties: Soil pH was determined by potentiometry; available nitrogen was determined by alkaline hydrolysis-diffusion method; available phosphorus was determined by molybdenum-antimony colorimetric method; available potassium was determined by flame photometry; and organic matter was determined by potassium dichromate-sulfuric acid thermal fusion method. The results are as follows: Figure 2 As shown, the contents of available nitrogen, available phosphorus, available potassium, and organic matter in the soil of the ginseng rotation group increased by approximately 36%, 25%, 36%, and 12%, respectively, compared with the continuous cropping control group, while the soil pH value decreased significantly from 7.5 to 6.2 (P < 0.05). Compared with Atractylodes lancea and Epimedium, ginseng rotation more effectively improved the fertility level and physicochemical properties of the soil in the Gastrodia elata continuous cropping group.

[0049] (3) Soil pest quantity determination: The number of nematodes and mites in a unit of soil was counted using a microscopic counting method. The results are as follows: Figure 3 As shown, the densities of nematodes and mites in the soil of ginseng rotation fields were reduced by 65.09% and 52.83%, respectively, compared with the continuous cropping control group. Microscopic observation revealed a significant reduction in the number of pests. Compared with Atractylodes lancea and Epimedium, ginseng rotation has a more significant inhibitory effect on soil pests caused by continuous ginseng cropping.

[0050] In conclusion, ginseng is a suitable crop rotation plant for restoring old Gastrodia elata fields.

[0051] Example 2: Isolation, identification, and functional verification of the beneficial bacterium *Pteris vittata* from ginseng rotation soil.

[0052] In this embodiment, a beneficial fungus was isolated from the rhizosphere soil of ginseng rotation and its function was verified.

[0053] 1. Isolation and Identification of Strains

[0054] A fungus, MS-GY-GLY, was isolated from the rhizosphere soil of a ginseng rotation group using a dilution coating method.

[0055] Select colonies with typical morphological characteristics ( Figure 4The ITS rDNA sequence was determined (see SEQ ID NO.1). Upon comparison, this strain showed a 99.8% similarity to *Mycena scorodonius*, and was named *Mycetinis scorodonius*. This strain was deposited on November 17, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, with accession number CGMCC No. 42371.

[0056] 2. Antagonistic pathogen experiment

[0057] Six pathogenic fungi of Gastrodia elata (Fusarium oxysporum, Fusarium redolens, Fusarium graminearum, Trichoderma harzianum, Trichoderma hamatum, and Trichoderma pyramidale) were isolated from soil samples obtained from continuous cropping of Gastrodia elata. The colony morphology was as follows: Figure 5 As shown. The above six pathogenic fungi of Gastrodia elata were prepared into bacterial suspensions after large-scale culture, and sprayed onto the stems of Gastrodia elata. After 14 days of incubation at room temperature, the results were observed. Figure 6 As shown, the tubers of Gastrodia elata all showed varying degrees of rot after being sprayed with six pathogens, and all six strains of bacteria were pathogenic to the tubers of Gastrodia elata.

[0058] The garlic-flavored small bark umbrella and the above six common pathogens of Gastrodia elata (Fusarium oxysporum, Fusarium redolens, Fusarium graminearum, Trichoderma harzianum, Trichoderma hamatum, and Trichoderma pyramidale) were confronted on PDA plates at 25°C.

[0059] The results are as follows Figure 7 As shown, *Trichoderma harzianum* exhibited varying degrees of inhibitory effects against the aforementioned pathogens. The highest inhibition rates were observed against *Trichoderma pallida* and *Trichoderma hookeriana*, at approximately 33% and 32%, respectively; the inhibition rate against *Fusarium oxysporum* was approximately 28%; the inhibition rates against *Fusarium graminearum* and *Fusarium aromaticum* were approximately 26% and 24%, respectively; and the lowest inhibition rate was against *Trichoderma harzianum*, at only 15%. Significant differences were observed among the treatments (P < 0.05). These results indicate that *Trichoderma harzianum* possesses the ability to inhibit the growth of pathogens, particularly showing a significant inhibitory effect against *Trichoderma* pathogens.

[0060] 3. Degradation of allelochemicals experiment

[0061] Garlic-flavored variegated caps were inoculated into a liquid culture medium containing p-hydroxybenzyl alcohol, dibutyl phthalate, and vanillin. After culturing at 25°C and 150 rpm for 7 days with shaking, the residual amount of allelochemicals in the culture medium was detected. The results are shown in Table 1.

[0062] Table 1. Degradation rate of allelochemicals of Gastrodia elata by garlic-flavored small umbrella peas during continuous cropping.

[0063]

[0064] Note: Data in the table are mean ± standard deviation. Different lowercase letters after the same indicator data indicate significant differences between treatment groups (P < 0.05).

[0065] Table 1 shows that the degradation rate of the three allelopathic substances by the garlic-flavored small umbrella was higher than 46%, which was significantly higher than that of the uninoculated control group (P < 0.05), indicating that it has the ability to efficiently degrade the allelopathic substances of Gastrodia elata after continuous cropping.

[0066] 4. Experiment on promoting the growth of Gastrodia elata and enhancing its medicinal components

[0067] In mid-May, during spring, *Gastrodia elata* was cultivated in soil continuously cropped under forest cover using the bag-planting method. Each bag, covered with *Armillaria mellea*, was laid flat, and two rows of inoculation holes were evenly punched at 45° and 135° angles, with a diameter of 1-3 cm and a depth of 2-4 cm. The perforated bags were then placed flat on a 5 cm high soil bed, and *Gastrodia elata* seedlings were inserted into the holes, with the buds facing outwards. The bags and seedlings were covered with 10 cm of rice husks, and then further covered with 3-10 cm of leaves. Before placing the *Gastrodia elata* seedlings, 2 mL of *Amanita muscaria* spore suspension (concentration 1×10⁻⁶) was injected into each inoculation hole in the experimental group. 8 (CFU / mL), the control group was injected with an equal volume of sterile water.

[0068] The agronomic traits and medicinal components of Gastrodia elata were determined after autumn harvest. The results are shown in Table 2.

[0069] Table 2. Effects of beneficial bacteria *Micrococcus garicus* on the production performance and active ingredients of *Gastrodia elata* grown in rotation with ginseng.

[0070]

[0071] Note: Data in the table are mean ± standard deviation. Different lowercase letters after the same indicator data indicate significant differences between treatment groups (P < 0.05).

[0072] Table 2 shows that the total weight, length, and width of *Gastrodia elata* produced from each spawn bag were significantly higher in the garlic-flavored small-skin group than in the control group (P<0.05). The garlic-flavored small-skin group significantly promoted the growth of continuously cropped *Gastrodia elata* and reduced the disease index. Furthermore, compared with the control group, the garlic-flavored small-skin group treatment significantly increased the content of gastrodin, p-hydroxybenzyl alcohol, barisonoside E, barisonoside B, barisonoside C, and the total amount of active ingredients (P<0.05). The garlic-flavored small-skin group significantly promoted the accumulation of dry matter and active ingredients in continuously cropped *Gastrodia elata*.

[0073] Example 3: Screening and application of plant-derived pesticides for resisting Gastrodia elata pests

[0074] This embodiment screened plant-derived pesticides that have highly effective repellent activity against Gastrodia elata pests.

[0075] 1. Preparation of plant-derived pesticides

[0076] Weigh out 50 g each of dried Sichuan pepper, chili pepper, tobacco leaves, garlic, mint, and mugwort, crush them, and place them in an Erlenmeyer flask.

[0077] Add 500 mL of 95% ethanol (solid-to-liquid ratio 1:10), extract by ultrasonication for 2 hours, filter and concentrate to obtain 100 mL of stock solution.

[0078] To use, take 25 mL of the stock solution and dilute it to 1 L with distilled water, mix well, and you will get a 0.25% concentration of plant-derived pesticide working solution. Spray it onto the cultivation substrate of Gastrodia elata bags.

[0079] 2. Assay for repellency activity

[0080] The tested pests were common nematodes and mites isolated from soil continuously cropped with Gastrodia elata. A two-well PDA plate method was used for the repulsion experiment. A 5 mm diameter well was made on one side of a 9 cm diameter PDA plate, and 50 μL of the aforementioned 0.25% plant-derived pesticide working solution was injected as a treatment well, with an equal amount of pyrethroid as a positive control. A well of the same size was made on the symmetrical other side, and 50 μL of distilled water was injected as a blank control well. 30-50 healthy, active insects were inoculated in the center of the plate. Each treatment was repeated 5 times. The inoculated plates were placed in an incubator at 25±1℃ and 70% relative humidity, and incubated in the dark for 2 hours. The number of live insects on the control and treatment sides was counted under a microscope. The repulsion index was calculated (repulsion index = number of insects on the control side / number of insects on the treatment side).

[0081] The results are as follows Figure 8 As shown, the repellent activity and lethality of extracts from Sichuan pepper, chili pepper, and tobacco against nematodes and mites were significantly higher than those from garlic, mint, and artemisia (P < 0.05). Therefore, Sichuan pepper, chili pepper, and tobacco are preferred as candidate biopesticides for subsequent field trials.

[0082] 3. Field efficacy verification

[0083] Gastrodia elata planting: In mid-May, in the soil of continuously cropped Gastrodia elata under forest cover, Gastrodia elata is planted using the bag method. Each bag covered with Armillaria mellea is laid flat, and two rows of inoculation holes are evenly punched at 45° and 135° angles, with a diameter of 1-3 cm and a depth of 2-4 cm. The perforated bags are then placed flat on a 5 cm high soil bed, and the white Gastrodia elata seedlings are inserted into the holes, with the buds facing outwards. The bags and seedlings are covered with 10 cm of rice husks, and then covered with 3-10 cm of leaves. Before placing the white Gastrodia elata seedlings, 2 mL of a suspension of Garlic-flavored Microsporum spores (concentration 1×10⁻⁶) is injected into each inoculation hole. 8 Based on this:

[0084] Biological pesticide treatment setup: Establish the following 5 treatment groups:

[0085] (1) Sichuan pepper group: spray with 0.25% Sichuan pepper extract working solution.

[0086] (2) Positive control group: sprayed with 0.25% high-efficiency cyhalothrin emulsifiable concentrate.

[0087] (3) Chili pepper group: Spray with 0.25% chili pepper ethanol extract working solution.

[0088] (4) Tobacco leaf group: Spray with 0.25% tobacco leaf ethanol extract working solution.

[0089] (5) Negative control group (CK): Sprayed with an equal amount of water.

[0090] Each treatment was configured with 3 replicates, and each replicate contained 5 substrate bags. Spraying was performed once at transplanting and again during the mid-growth phase (July), with each application evenly applied to the surface of the substrate bags and the surrounding substrate at a rate of 100 mL / m². 2 .

[0091] Measurement indicators and results:

[0092] a) Agronomic traits and yield of Gastrodia elata: determined post-harvest. Table 3 shows that the total weight, tuber length, and width of Gastrodia elata produced from a single bag in the Sichuan pepper treatment group were significantly higher than those in other groups (P < 0.05), and the disease index was the lowest. Furthermore, the Sichuan pepper group had the highest dry matter content (P < 0.05), indicating that it was beneficial for dry matter accumulation.

[0093] b) Medicinal components of Gastrodia elata: The main medicinal components of Gastrodia elata were determined by high performance liquid chromatography. The results (Table 3) showed that the contents of gastrodin, p-hydroxybenzyl alcohol, barisonoside E, barisonoside B, and barisonoside C in the zanthoxylum bungeanum treatment group, as well as the sum of the contents of gastrodin and p-hydroxybenzyl alcohol and the total amount of medicinal components, were significantly higher than those in the negative control group and other treatment groups (P < 0.05).

[0094] c) Soil pest count: Soil samples were collected before harvest, and nematodes and mites were counted using the Bellman funnel method. Results ( Figure 9 The results showed that the number of soil nematodes and the total number of mites and nematodes in the pepper-treated group were not significantly different from those in the chemical pesticide (pyrethroid group), and were significantly lower than those in other plant-derived pesticide groups and the negative control group (P < 0.05). Specifically, compared with the negative control group, the pepper treatment reduced the number of nematodes by 43.75%, and the reduction in the total number of mites and nematodes was the most significant.

[0095] Table 3. Effects of plant-derived pesticides against pests in Gastrodia elata on the active components of continuously cropped Gastrodia elata.

[0096]

[0097] Note: Data in the table are mean ± standard deviation. Different lowercase letters after the same indicator data indicate significant differences between treatment groups (P < 0.05).

[0098] Example 4: Screening and Validation of Ginseng-Inspired Crop Rotation Substrate

[0099] This embodiment screens cultivation substrate formulations suitable for repairing continuous cropping obstacles in Gastrodia elata.

[0100] 1. Substrate setup and cultivation

[0101] A total of 6 different matrix formulations were set up:

[0102] SF3:1 (sand:fermented rice husk volume ratio 3:1);

[0103] SF2:1 (sand:fermented rice husk volume ratio 2:1);

[0104] SF1:1 (sand:fermented rice husk volume ratio 1:1).

[0105] SJ3:1 (sand:sawdust volume ratio 3:1);

[0106] FD (100% fermented rice husk);

[0107] DK (100% unfermented rice husk).

[0108] Each treatment group consisted of 5 bags as one replicate, and the replicates were performed 5 times.

[0109] Gastrodia elata planting: In mid-May, in non-forest land continuously cropped with Gastrodia elata, the bag method is used to plant Gastrodia elata. Each bag covered with Armillaria mellea is laid flat, and two rows of inoculation holes are evenly punched at 45° and 135° angles, with a diameter of 1-3 cm and a depth of 2-4 cm. The perforated bags are then placed flat on a 5 cm high soil bed, and the white Gastrodia elata seedlings are inserted into the holes, with the buds facing outwards. Using the six different substrate formulas mentioned above, each bag and seedling is covered with a 10 cm layer of the six different substrates. Then, 3-10 cm of leaves are placed on top of the substrate. Before placing the white Gastrodia elata seedlings, 2 mL of garlic-flavored Amanita muscaria spore suspension (concentration 1×10⁻⁶) is injected into each inoculation hole. 8 (CFU / mL).

[0110] 2. Results and Analysis

[0111] Production performance: Table 4 shows that in terms of total weight of Gastrodia elata, the SJ3:1 group was significantly higher than the DK group, the DK group was higher than the FD group, the FD group was higher than the SF3:1 group, the SF3:1 group was higher than the SF2:1 group, and the SF2:1 group was higher than the SF1:1 group (all P < 0.05). The length of Gastrodia elata was highest in the SJ3:1 group and lowest in the SF1:1 group (P < 0.05), with no significant differences among the other groups. The width of Gastrodia elata was higher in the SJ3:1, FD, and DK groups, with the DK group having the lowest width (P < 0.05), and no significant differences among the other groups. The SJ3:1 matrix showed the best performance, while the FD and DK groups improved the dry weight of Gastrodia elata.

[0112] Pharmacological components: Table 5 shows that the contents of gastrodin, bacilin B, bacilin C, and their combined content were significantly higher in the DK and FD groups than in the SJ3:1 group (P < 0.05), while the SJ3:1 group was significantly higher than all other groups. p-Hydroxybenzyl alcohol was highest in the SF3:1 group, significantly higher than in the SJ3:1 and DK groups, and the latter two were higher than in other groups. Bacilin E was relatively high in the SJ3:1, SF1:1, and DK groups; bacilin A was highest in the FD group and lowest in the SF3:1 group. In terms of the total pharmacodynamic components, the DK, FD, and SJ3:1 groups were significantly higher than the SF2:1 and SF1:1 groups, while the SF3:1 group was the lowest. In summary, the FD and DK groups showed the best effect in improving the pharmacodynamic components, followed by the SJ3:1 group.

[0113] Considering both production performance and medicinal properties, SJ3:1 (sand:sawdust = 3:1) and FD (fermented rice husk) are the preferred substrates for repairing the continuous cropping obstacles of Gastrodia elata.

[0114] Table 4. Verification of the production performance of Gastrodia elata by continuous cropping using a ginseng-inspired crop rotation cultivation substrate.

[0115]

[0116] Note: Data in the table are mean ± standard deviation. Different lowercase letters after the same indicator data indicate significant differences between treatment groups (P < 0.05).

[0117] Table 5. Verification of the medicinal components of Gastrodia elata grown in rotational ginseng cultivation substrate.

[0118]

[0119] Note: Data in the table are mean ± standard deviation. Different lowercase letters after the same indicator data indicate significant differences between treatment groups (P < 0.05).

[0120] The method for repairing and reducing continuous cropping obstacles in Gastrodia elata provided by this invention is comprehensive and highly effective. The selected ginseng rotation model, beneficial microorganism *Pteris vittata*, plant-derived pesticide *Zanthoxylum bungeanum* extract, and optimized cultivation substrate together constitute a highly efficient and environmentally friendly comprehensive solution for repairing continuous cropping obstacles. This method has clear steps and well-defined parameters, making it particularly suitable for large-scale bag cultivation of *Gastrodia elata* in the Changbai Mountain region. It can be widely promoted and applied in Chinese medicinal herb planting enterprises, and is of great value in ensuring the sustainable development of the Gastrodia elata industry.

[0121] Garlic-flavored Mycena purpureofusca ITS rDNA sequence (SEQ ID NO.1):

[0122] .

[0123] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A garlic-flavored mini umbrella, characterized in that, The garlic-flavored mini umbrella is classified as garlic-flavored mini umbrella ( Mycetinis scorodonius It was deposited on November 17, 2025, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 42371.

2. A microbial inoculant, characterized in that, It includes the garlic-flavored mini umbrella as described in claim 1.

3. The application of the garlic-flavored small umbrella as described in claim 1 or the microbial agent as described in claim 2, characterized in that, The application includes any one of the following (1) to (4): (1) Inhibits the growth of pathogenic bacteria causing Gastrodia elata; (2) Degrading allelochemicals in soil where Gastrodia elata is continuously cropped; (3) Promote the growth of Gastrodia elata or increase the content of medicinal components in Gastrodia elata; (4) Prepare products for repairing and reducing the obstacles of continuous cropping of Gastrodia elata; The pathogenic fungi are Fusarium oxysporum, Fusarium aromaticum, Fusarium graminearum, Trichoderma harzianum, Trichoderma hookeriana, and Trichoderma tarda; The allelochemicals are p-hydroxybenzyl alcohol, dibutyl phthalate and vanillin; The active ingredients of Gastrodia elata are gastrodin, p-hydroxybenzyl alcohol, barisonoside E and barisonoside B.

4. A method for repairing and reducing the obstacles of continuous cropping of Gastrodia elata by imitating ginseng crop rotation, characterized in that, The method includes the step of applying the garlic-flavored small umbrella as described in claim 1 or the microbial agent as described in claim 2 to a continuous cropping Gastrodia elata cultivation system.

5. The method of claim 4, wherein, It also includes the step of applying a 0.25% (w / v) concentration of Sichuan pepper ethanol extract to a continuous cropping Gastrodia elata cultivation system.

6. The method according to claim 5, characterized in that, The preparation method of the Sichuan pepper ethanol extract includes ultrasonic extraction of Sichuan pepper powder with 95% ethanol at a material-to-liquid ratio of 1g:10mL, followed by filtration and concentration to obtain the Sichuan pepper ethanol extract.

7. The method according to claim 4, characterized in that, The cultivation substrate of the continuous cropping Gastrodia elata cultivation system is composed of sand and sawdust in a volume ratio of 3:1, or of 100% fermented rice husks.

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