Method for identifying abundance of cholinesterase in duck plasma and application of related molecular marker

By PCR amplification at specific sites on the duck reference genome, the genotype of ducks can be determined, solving the problem of screening for cholinesterase abundance in duck plasma. This method improves the growth performance and health of meat ducks, reduces breeding costs, and is applicable to Sino-New Zealand white-feathered meat ducks and other duck breeds.

CN121472414BActive Publication Date: 2026-07-24INSTITUTE OF ANIMAL SCIENCES OF CHINESE ACADEMY OF AGRICULTURAL SCIENCES +1
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Patent Information

Application Number
CN202511631343.2
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-11-10
Publication Date
2026-07-24
Estimated Expiration
2045-11-10

AI Technical Summary

Technical Problem

How can we use genetic breeding techniques to screen key SNP sites that affect the abundance of cholinesterase in duck plasma, apply them to marker-assisted selection, improve the growth performance and health of meat ducks, and meet market demand?

Method used

PCR amplification was performed on a DNA fragment at the 3516189th polymorphic site (starting from the 5' end) on chromosome 9 of the duck reference genome IASCAAS_PekinDuck_T2T to determine the CC, CT, and TT genotypes of ducks. Genotype screening was used to influence the abundance of cholinesterase in duck plasma, and molecular marker-assisted selection was performed.

Benefits of technology

This method enables early screening of cholinesterase abundance in duck plasma, reducing feed waste, lowering breeding costs, and improving selection accuracy. It is suitable for automated detection in large-scale breeding populations and has industrial application value.

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Abstract

The application discloses a method for identifying cholinesterase abundance of duck plasma and application of a related molecular marker, relates to the technical field of biology, and comprises the following steps: using a primer pair to amplify a DNA fragment containing a polymorphic site at the 3516189th base from the 5' end on chromosome No. 9 of a duck reference genome IASCAAS_PekinDuck_T2T; the duck reference genome IASCAAS_PekinDuck_T2T is a duck reference genome sequence in a GenBank database; the duck is a Zhongxinbaihu meat duck; the primer pair is composed of a DNA molecule shown in SEQ ID No. 2 and a DNA molecule shown in SEQ ID No. 3; and the plasma cholinesterase abundance of the duck with a CC genotype and a CT genotype is significantly higher than that of the duck with a TT genotype (P<0.05). According to the application, the genotype of the duck is determined by detecting the genomic DNA of the duck, early screening of the cholinesterase abundance of the plasma of the duck is realized, and the breeding cost can be reduced.
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