Chalcone isoprenyl transferase gene GiPT16, GiPT16 protein, amplification primer set and application

By identifying the chalcone isopentenyltransferase GiPT16 in Glycyrrhiza uralensis, we have achieved the efficient synthesis of licorice chalcone compounds, solving the problem of high resource dependence in existing technologies and promoting the green development and industrial application of traditional Chinese medicine resources.

CN121518510BActive Publication Date: 2026-05-12INSTITUTE OF CHINESE MATERIA MEDICA CHINA ACADEMY OF CHINESE MEDICAL SCIENCES
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Patent Information

Application Number
CN202610055633.5
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2026-01-16
Publication Date
2026-05-12
Estimated Expiration
2046-01-16

AI Technical Summary

Technical Problem

Existing technologies make it difficult to efficiently and sustainably obtain glycyrrhizin chalcone compounds. In particular, the isopentenylation modification pathway of glycyrrhizin chalcone in Glycyrrhiza inflata has not been fully studied, resulting in high resource dependence, high cost, and difficulty in meeting the demand for high-value development.

Method used

The GiPT16 gene and its protein of chalcone isopentenyltransferase in Glycyrrhiza inflata were discovered and identified. Through synthetic biology, the heterologous synthesis of glycyrrhizin chalcone compounds was achieved in microorganisms or plants. The GiPT16 catalyzes the production of psoralen and glycyrrhizin C from the substrate isoglycyrrhizin.

Benefits of technology

This enables the sustainable and efficient synthesis of licorice chalcone compounds, reducing reliance on wild resources and promoting the green development and industrial application of traditional Chinese medicine resources.

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Abstract

This invention provides a chalcone isopentenyltransferase gene. GiPT16 This invention relates to the GiPT16 protein, amplification primer set, and applications, belonging to the field of biogenetics technology. It is based on *Glycyrrhiza inflata* (GiPT16 protein, amplification primer set, and applications). Glycyrrhiza inflata Using whole-genome sequencing data and a reverse genetics strategy, the key aromatic isopentenyltransferase GiPT16, involved in the isopentenylation modification of chalcone active ingredients, was successfully identified and functionally characterized. This enzyme was confirmed to specifically catalyze the biosynthesis of psoralen and glycyrrhizin C using DMAPP as a donor. It is the first chalcone isopentenyltransferase characterized in *Glycyrrhiza inflata*, filling a gap in the study of key enzymes in the chalcone isopentenylation metabolic pathway of this species and providing important evidence for further elucidating the molecular mechanisms of quality formation in *Glycyrrhiza inflata* medicinal materials.
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