Construction method of drosophila embryo permeabilization model
By treating Drosophila embryos with Citrasolv solution and bleaching agent solution, a highly permeable and viable Drosophila embryo permeation model was constructed, solving the toxicity problem of embryo removal caused by the wax layer in traditional methods, and enabling rapid and effective drug screening and toxicological evaluation.
Patent Information
- Application Number
- CN202511776026.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-11-28
- Publication Date
- 2026-02-17
AI Technical Summary
Existing technologies struggle to effectively remove the waxy layer to improve permeability while maintaining embryo viability and survival rate when constructing fruit fly embryo models. Traditional solvents such as heptane and octane are toxic and harmful to embryos.
Drosophila embryos were permeated with Citrasolv solution (mainly composed of limonene and surfactant). The waxy layer was removed by dilution to a ratio of 1:30 and treatment time of 2 minutes. The villous membrane layer was removed by bleaching solution, thus constructing a Drosophila embryo permeation model.
It achieves high permeability and high survival rate of Drosophila embryos, making them suitable for drug screening and toxicological evaluation. It enables rapid preparation of batches of permeable embryos, reduces damage to the embryos, and improves the efficiency and accuracy of drug screening.