InDel molecular marker primer group for identifying variety of orchis polyphylla and application of InDel molecular marker primer group

By developing the InDel molecular marker primer set and high-throughput sequencing technology for Woyang moss, a DNA fingerprint database was constructed, solving the problems of time-consuming, labor-intensive, and inaccurate identification of Woyang moss varieties. This enabled rapid and accurate identification of varieties and purity, which is applicable to seed testing, variety registration, and market supervision.

CN121538342APending Publication Date: 2026-02-17ANHUI SCI & TECH UNIV
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Patent Information

Application Number
CN202511867588.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-12-11
Publication Date
2026-02-17

AI Technical Summary

Technical Problem

Existing technologies rely on morphological characteristics for the identification of dried moss varieties in Woyang, which is time-consuming, labor-intensive, and yields inaccurate results, making it difficult to achieve rapid and accurate identification of varieties and purity.

Method used

A primer set containing 40 pairs of InDel molecular markers was developed. A dry DNA fingerprint database of *Lysimachia foenum-graecum* was constructed by PCR amplification and high-throughput sequencing. The authenticity and purity of the variety were identified by utilizing the length polymorphism of the InDel markers.

Benefits of technology

It enables rapid and accurate identification of the Woyang moss variety, reduces the impact of environmental factors, saves land and human resources, and provides key technical support for variety rights protection and new variety breeding.

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Abstract

The invention belongs to the technical field of molecular markers, and in particular relates to an InDel molecular marker primer group for identifying varieties of Taiwan ivy mosses and application of the InDel molecular marker primer group for identifying the varieties of Taiwan ivy mosses. The InDel molecular marker primer group comprises 40 pairs of primers, and the nucleotide sequences of the primers are as shown in SEQ ID NO. 1 to SEQ ID NO. 80. The primer group is developed on the basis of a high-throughput sequencing technology, InDel fragment length data is obtained by performing PCR amplification and high-throughput sequencing on genome DNA of a sample to be detected, and authenticity identification and purity identification of the variety of the Taiwan drymoglossum are realized by comparing a difference marker number with a standard sample. The method overcomes the defects that a traditional morphological identification method is time-consuming and labor-consuming and is influenced by the environment, has the advantages of being rapid in detection, high in accuracy, high in flux and the like, and is suitable for germplasm resource protection and new variety registration and identification of the Taiwan dryopteris.
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