Preparation method and application of chlorella exosome and mRNA complex nanoparticles

By using Chlorella exosomes as carriers and combining them with electroporation to prepare mRNA composite nanoparticles, the problems of stability and low cellular uptake efficiency of existing mRNA delivery systems have been solved, realizing safe and scalable mRNA delivery and expanding its application in the biomedical field.

CN121555319BActive Publication Date: 2026-06-02NINGBO UNIV
1 Cites 0 Cited by

Patent Information

Application Number
CN202610079802.9
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2026-01-21
Publication Date
2026-06-02
Estimated Expiration
2046-01-21

AI Technical Summary

Technical Problem

Existing mRNA delivery systems suffer from problems such as poor stability, low cellular uptake efficiency, strong immunogenicity, and delivery difficulties, which limit their widespread application in the biomedical field. In particular, exosomes derived from mammalian cells have limitations such as low yield, complex extraction process, and high cost.

Method used

Using Chlorella exosomes as carriers, mRNA composite nanoparticles were prepared under specific culture conditions and by electroporation. Taking advantage of the natural targeting and biocompatibility of Chlorella exosomes, targeted delivery and intracellular expression of mRNA were achieved.

Benefits of technology

This has enabled the safe and scalable production of mRNA, improved intracellular expression efficiency and stability, reduced immune rejection and toxic side effects, and broadened the application prospects of mRNA in vaccines, tumor immunology and gene therapy.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The application discloses a preparation method and application of chlorella exosome and mRNA composite nanoparticles, and has the characteristics that chlorella is inoculated in BG11 culture medium and cultured for 6-8 days, then inoculated in BG11 culture medium added with glucose and cultured, on the second day of the culture, exogenous stress is given or no treatment is given, finally, chlorella algae liquid is collected after culturing for 5-6 days, and chlorella exosome is obtained through differential centrifugation; the exosome is mixed with mRNA at a mass ratio of 1:1-1:3, an electroporation buffer is added, and the mixed liquid in a colorimetric dish is subjected to electroporation through an electroporation instrument, and the collected precipitated particles are the mRNA composite nanoparticles of the chlorella exosome, and the chlorella exosome has the effects of enhancing CAT and SOD in vitro and / or antagonizing MDA, in vitro antioxidant property and in vivo oxidative stress reliever of zebra fish, and can be used as a carrier to load and protect mRNA, so that targeted delivery and intracellular expression are realized.
Need to check novelty before this filing date? Find Prior Art