Recombinant turkey herpesvirus as well as preparation method and application thereof

By inserting a tandem epitope expression cassette into turkey herpesvirus, a recombinant turkey herpesvirus was constructed. Combined with intraembryonic and subcutaneous immunization with H9N2 inactivated vaccine, the problem of insufficient immune protection in chicks was solved, and effective challenge protection was achieved within 3 days.

CN121628845APending Publication Date: 2026-03-10SOUTH CHINA AGRICULTURAL UNIVERSITY +1
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Patent Information

Application Number
CN202511486376.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-10-17
Publication Date
2026-03-10

AI Technical Summary

Technical Problem

Existing avian influenza vaccines are difficult to induce an effective cellular immune response in chicks, and there is a lack of intraembryonic immunization vaccines to prevent H9N2 AIV infection, making chicks susceptible to avian influenza infection in the later stages of hatching. Existing combined immunization programs cannot provide complete protection within 3 days.

Method used

Recombinant turkey herpesvirus (rHVT-BNT) was constructed. By inserting a tandem epitope expression cassette into the HVT vector, multiple B cell and T cell epitopes were expressed. Combined with intraembryonic and subcutaneous immunization with H9N2 inactivated vaccine, a strong humoral and cellular immune response was elicited.

Benefits of technology

It significantly increases HI antibody and neutralizing antibody titers within 3 days, providing a clear protection against viral challenge, which is superior to existing protocols and is suitable for immune protection in chicks.

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Abstract

The invention belongs to the technical field of biology, and discloses a recombinant turkey herpesvirus, and a tandem epitope expression cassette is inserted between UL45 and UL46 of the recombinant turkey herpesvirus; the tandem epitope expression cassette is used for expressing a plurality of B cell epitopes and a plurality of T cell epitopes. According to the recombinant herpesvirus of turkeys, a tandem epitope is inserted into an HVT (herpesvirus of turkeys) vector, and experiments prove that the recombinant herpesvirus of turkeys can induce higher HI antibody and neutralizing antibody titer, obviously stimulate spleen T lymphocyte response of immunized chicken, and can generate an obvious challenge protection effect in 3 days; the challenge protection effect is obviously superior to that of a combined immunization scheme of H9N2 AIV multi-epitope recombinant baculovirus (BV-BNT) and InV provided by the applicant; meanwhile, the recombinant turkey herpesvirus is verified to be capable of performing intraembryonic vaccination and generating an obvious immune protection effect. Meanwhile, the invention further provides a preparation method and application of the recombinant turkey herpesvirus.
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