Multiple fluorescent staining method for multiple protein targets

By using multiple fluorescent dye combinations to stain multiple protein targets on the same tissue section, the limitations on the number of targets and the error problems in the existing technology are solved, and a highly efficient multiplex fluorescence staining effect is achieved.

CN121656554APending Publication Date: 2026-03-13QIAGEN SUZHOU TRANSLATIONAL MEDICINE CO LTD
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Patent Information

Application Number
CN202411229379.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-09-03
Publication Date
2026-03-13

AI Technical Summary

Technical Problem

Current multiplex immunohistochemistry techniques can only label and image up to 6 target sites on the same slide. When there are more than 6 target sites, it is necessary to stained slides consecutively and then overlay the images or repeat the elution process, which leads to errors and antigen loss.

Method used

Multiple fluorescent dye combinations are used to stain the test samples. Each combination includes two fluorescent dyes with different emission wavelengths. Multiple protein targets are directly labeled on the same tissue section. The difference in emission wavelength of the fluorescent dye combination is used to avoid cross-coloring and achieve multiple fluorescence staining.

Benefits of technology

It significantly increases the number of stainable protein targets in tissue sections, simplifies the staining process, avoids errors caused by differences in section continuity and repeated elution, saves the number of sections, and reduces antigen loss.

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Abstract

The invention discloses a multiple fluorescent staining method for multiple protein targets, and relates to the technical field of multiple immunofluorescent staining. According to the method provided by the invention, the number of dyeable protein targets in the tissue slice can be greatly increased, multiple fluorescent staining steps can be simplified, and by adopting the method provided by the invention, imaging can be carried out after all the targets are directly stained on the basis of one tissue slice. The number of tissue slices required by dyeing imaging is reduced, dyeing of more than 6 protein targets can be completed by only one tissue slice, and errors caused by slice continuity differences are avoided. In addition, repeated elution and re-dyeing are not needed, so that the problems of antigen loss and weakening and the like caused by repeated elution are avoided.
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