Microsphere suspension detection device and method
By detecting the suspension time of microsphere suspensions using laser signals, the problem of subjective error caused by human visual observation is solved, enabling accurate evaluation and efficient detection of microsphere suspension performance.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- CARDIOLINK SCI (SHENZHEN) MEDICAL TECH DEV CO LTD
- Filing Date
- 2025-12-29
- Publication Date
- 2026-07-24
AI Technical Summary
In existing technologies, the detection of microsphere suspension time relies on human visual observation, which leads to large subjective judgment errors and makes it impossible to accurately assess suspension performance.
A laser emitter emits pulse signals to the microsphere suspension, a signal receiver receives the reflected signals, a data processing component calculates the ratio of the cumulative signal amount to the total reflected signal amount, and a data output component obtains the suspension time based on the ratio.
Accurately detect the suspension time of microspheres, avoid subjective errors, and improve detection efficiency and stability.
Smart Images
Figure CN121702961B_ABST
Abstract
Description
Technical Field
[0001] This application relates to the field of suspension measurement technology, and in particular to a device and method for detecting the suspension properties of microspheres. Background Technology
[0002] Embolization microspheres are tiny particles widely used in interventional radiology and vascular surgery as an important tool for endovascular embolization therapy. In clinical use, to facilitate procedure and observe treatment effects, microspheres are usually mixed with contrast agents before injection. After mixing with the contrast agent, the microspheres are uniformly dispersed in the system for a period of time. Uniform microspheres are beneficial for interventional procedures and reduce the risk of catheter occlusion. Therefore, suspension time, as an important evaluation method for characterizing the suspension performance of microspheres, is widely used.
[0003] Generally, the suspension time of microspheres is recorded by observing the stratification of microspheres in a microsphere suspension with the naked eye. The longer the suspension time, the better the suspension performance of the microspheres.
[0004] However, since microspheres do not create a clear boundary line in the liquid during the floating and sinking process, it is impossible to effectively assess the time point when the microsphere suspension reaches the stratification point. Therefore, whether the microspheres have reached the end of the timing depends on human subjective judgment, which leads to a large systematic test error in the suspension test. Summary of the Invention
[0005] To address the aforementioned technical problems, this application provides a microsphere suspension detection device and method.
[0006] In a first aspect, this application provides a microsphere suspension detection device, comprising: Lofting module and detection module; The sampling module includes a test container, which is used to hold the microsphere suspension. The detection module includes a laser emitter, a signal receiver, a data processing component, and a data output component; The laser emitter is used to emit pulse signals to the microsphere suspension in the test container and to send the emission time of the pulse signals to the data processing component; The signal receiver is used to receive the reflected signal obtained by the microspheres in the microsphere suspension reflecting the pulse signal, and to send the reception time of the reflected signal to the data processing component; The data processing component is used to obtain the cumulative signal amount returned by the microspheres above a preset height and the total reflected signal amount returned by the microspheres in the entire height range based on the transmission time, the reception time, and the signal strength of the reflected signal, and to send the ratio of the cumulative signal amount to the total reflected signal amount as a real-time detection value to the data output component. The data output component is used to obtain the hover time based on the real-time detection value and the preset detection threshold.
[0007] Secondly, this application provides a method for detecting the suspension of microspheres, the method comprising: S1; After determining that the microsphere suspension to be tested has the property of downward sedimentation, the microsphere suspension detection device is placed upright, so that the detection module of the microsphere suspension detection device is located on the upper side of the sampling module, wherein the upper side is opposite to the direction of gravity. S2: Input the preset detection threshold into the data output component of the microsphere suspension detection device; S3: After placing the well-shaken microsphere suspension into the test container of the microsphere suspension detection device, immediately press the start button in the microsphere suspension detection device, and the display screen of the microsphere suspension detection device will start timing. S4: The laser emitter of the microsphere suspension detection device emits a pulse signal to the microsphere suspension, and the signal receiver of the microsphere suspension detection device receives the reflected signal obtained by the microspheres in the microsphere suspension reflecting the pulse signal; S5: The data processing component of the microsphere suspension detection device obtains real-time detection values based on the emission time of the pulse signal, the reception time of the reflected signal, and the signal strength of the reflected signal; S6: The data output component of the microsphere suspension detection device determines whether the real-time detection value is less than or equal to the detection threshold. If so, the display screen stops timing, and the current time displayed on the display screen is the suspension time of the microsphere suspension.
[0008] The microsphere suspension detection device and method provided in this application emits a pulse signal to the microsphere suspension in the test container via a laser emitter. A signal receiver receives the reflected signal obtained by the microspheres reflecting the pulse signal. A data processing component obtains the cumulative signal returned by the microspheres above a preset height and the total reflected signal returned by the microspheres throughout the entire height range based on the emission time, the reception time, and the signal intensity of the reflected signal. The ratio of the cumulative signal to the total reflected signal is used as a real-time detection value. A data output component obtains the suspension time based on the real-time detection value and a preset detection threshold. This avoids subjective judgment by the operator, accurately detects the suspension time of the microspheres, reduces detection errors, and improves detection efficiency. Attached Figure Description
[0009] To more clearly illustrate the technical solutions of this application, the accompanying drawings used in the embodiments will be briefly described below. It should be understood that the following drawings only show some embodiments of this application and should not be regarded as a limitation on the scope of protection of this application. In the various drawings, similar components are numbered similarly.
[0010] Figure 1 A schematic diagram of a test scenario for existing technologies is shown; Figure 2 A schematic diagram of the microsphere suspension detection device provided in this application is shown; Figure 3 A schematic flowchart of the microsphere suspension detection method provided in this application is shown; Figure 4 This paper shows a schematic diagram of the microsphere suspension detection device provided in this application in a tilted-to-one state; Figure 5 This diagram shows another side-tilted state of the microsphere suspension detection device provided in this application; Figure 6 This invention provides a schematic diagram of a microsphere suspension detection device filled with a suspension. Figure 7 This paper shows a schematic diagram of the suspension mixing state of the microsphere suspension detection device provided in this application; Figure 8 This application provides a schematic diagram of the total reflected signal quantity. Figure 9 A schematic diagram of the sedimentation state of the suspension in the microsphere suspension detection device provided in this application is shown. Figure 10 Another schematic diagram of the total reflected signal quantity provided in this application is shown; Figure 11Another schematic diagram of the microsphere suspension detection method provided in this application is shown. Detailed Implementation
[0011] The technical solutions of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments.
[0012] The components of this application, typically described and illustrated in the accompanying drawings, can be arranged and designed in a variety of different configurations. Therefore, the following detailed description of embodiments of this application provided in the drawings is not intended to limit the scope of the claimed application, but merely to illustrate selected embodiments of the application. All other embodiments obtained by those skilled in the art based on the embodiments of this application without inventive effort are within the scope of protection of this application.
[0013] In the following, the terms “comprising,” “having,” and their cognates, which may be used in various embodiments of this application, are intended only to indicate a particular feature, number, step, operation, element, component, or combination thereof, and should not be construed as excluding, firstly, the presence of one or more other features, numbers, steps, operations, elements, components, or combinations thereof, or adding the possibility of one or more features, numbers, steps, operations, elements, components, or combinations thereof.
[0014] Furthermore, the terms "first," "second," and "third" are used only to distinguish descriptions and should not be interpreted as indicating or implying relative importance.
[0015] Unless otherwise specified, all terms used herein (including technical and scientific terms) shall have the same meaning as commonly understood by one of ordinary skill in the art to which the various embodiments of this application pertain. Terms (such as those defined in commonly used dictionaries) shall be interpreted as having the same meaning as in their contextual meaning in the relevant technical field and shall not be construed as having an idealized or overly formal meaning, unless clearly defined in the various embodiments of this application.
[0016] In existing technologies, the suspension performance of embolic microspheres is measured by calculating the suspension time from the moment the microspheres are mixed until they float or sink to a point where they occupy two-thirds of the liquid volume. See also... Figure 1In practical applications, a microsphere suspension is introduced into container 100, and the human eye 200 continuously observes whether the microsphere stratification interface in the microsphere suspension has dropped to 2 / 3 of the microsphere suspension volume. Because there is no clear boundary between the microspheres and the liquid surface during the sedimentation process, whether the microspheres have reached the timing endpoint depends on the human's subjective judgment, leading to systematic errors during the test. Furthermore, different operators may have different judgments, resulting in an insufficiently objective assessment.
[0017] In view of this, this application provides a microsphere suspension detection device, which can accurately detect the suspension time of microspheres through the set sampling module and detection module, avoiding the subjective influence of operators and reducing errors.
[0018] See Figure 2 The microsphere suspension detection device includes: a sampling module 82 and a detection module 81; wherein, the sampling module 82 includes a test container 5, and the detection module 81 includes a laser emitter 3, a signal receiver 4, a data processing component 2, and a data output component. Figure 1 (Not shown). The test container 5 is used to hold the microsphere suspension. The laser emitter 3 is used to emit a pulse signal into the microsphere suspension in the test container 5 and send the emission time of the pulse signal to the data processing component 2. The signal receiver 4 is used to receive the reflected signal obtained by the microspheres in the microsphere suspension reflecting the pulse signal, and send the reception time of the reflected signal to the data processing component 2; the data processing component 2 is used to obtain the cumulative signal amount returned by the microspheres above a preset height and the total reflected signal amount returned by the microspheres in the entire height range according to the emission time, the reception time, and the signal strength of the reflected signal, and send the ratio of the cumulative signal amount to the total reflected signal amount as a real-time detection value to the data output component. The data output component is used to obtain the suspension time according to the real-time detection value and a preset detection threshold.
[0019] In this embodiment, the microsphere suspension detection device can be a sealed cylindrical container divided into an experimental area and a detection area. The experimental area mainly consists of the sample placement module 82, and the detection area mainly consists of the detection module 81. The sample placement module 82 is located at one end of the microsphere suspension detection device, and the detection module 81 is located at the other end. A mixed solution of microspheres and contrast agent is added to the test container 5 of the sample placement module 82 and mixed thoroughly. Then, the detection module 81 starts timing, during which the laser emitter 3 of the detection module 81 continuously emits pulse signals, and the emission time of the pulse signals is recorded. After the pulse signal contacts the microspheres, it reflects a certain reflected signal, and the signal intensity of the reflected signal increases with the increase in the number of microspheres. The returned reflected signal is received by the signal receiver 4. Based on the different return times and intensities of the reflected signals, the detection module 81 can determine the number of microspheres at different liquid levels.
[0020] In this embodiment, the cumulative signal quantity returned by the microspheres above a preset height is obtained based on the emission time, the reception time, and the signal strength of the reflected signal. The total reflected signal quantity returned by the microspheres across the entire height range is obtained based on the emission time, the reception time, and the signal strength of the reflected signal. The ratio of the cumulative signal quantity to the total reflected signal quantity is used as a real-time detection value. The time between the change of the real-time detection value and a preset detection threshold is recorded as the suspension time of the microspheres. A longer suspension time indicates better microsphere suspension performance. The suspension time obtained by this microsphere suspension detection device avoids the influence of subjective factors, has high stability, and is a repeatable result.
[0021] In this embodiment, the laser emitter 3 can continuously emit pulse signals and synchronize the transmission time of the pulse signals to the data processing component 2. The signal receiver 4 can receive the signal reflected by the microsphere after the pulse signal is irradiated, and continuously receive the reflected signal, synchronizing the reflected signal and the reception time to the data processing component 2.
[0022] In one embodiment, the data output component is further configured to determine whether the real-time detection value is less than or equal to the detection threshold; if so, the hover time is determined based on the initial time of starting the test and the current time.
[0023] In this embodiment, the data output component obtains the preset detection threshold in the following way: the data processing component 2 receives an empirically preset threshold input by the user and determines the preset threshold as the detection threshold. The detection threshold can also be obtained in the following way: using a microsphere suspension detection device to test the detection threshold. Exemplarily, this includes the following steps: selecting a standard microsphere suspension and placing the standard microsphere suspension into the test container 5; when the layered interface in the test container 5 is determined to be at a preset height, obtaining the total standard reflected signal returned by microspheres at different heights in the standard microsphere suspension, and the standard cumulative signal returned by microspheres above the preset height in the standard microsphere suspension; determining the signal ratio of the standard cumulative signal to the total standard reflected signal as the detection threshold. This preset height is determined according to actual conditions; for example, the preset height is 1 / 3 or 2 / 3 of the total height of the test container 5, and is not limited here.
[0024] The following details the detection logic of data processing component 2 for acquiring reflected and accumulated signal quantities.
[0025] In this embodiment, the data processing component 2 is used to integrate the relative signal intensity returned by microspheres at different heights in the microsphere suspension over the entire height range to obtain the total reflected signal quantity.
[0026] As an example, the total reflected signal can be calculated using the following formula.
[0027]
[0028] in, Let H represent the total reflected signal quantity, H represent the height of the test container, x∈[0,H], and I(x) represent the relative signal intensity of x in different liquid level height ranges.
[0029] It is understandable that the height of the test container can be selected based on actual conditions. For example, if the height H of the test container is 15ml, and it is filled with microsphere suspension, the detection height of the microsphere suspension is 15ml. This 15ml height is divided into n equal intervals i to i+j, with each interval having a height of j. The height of j is determined by the detection accuracy of the signal receiver 4, typically 1mm. The signal I(j) reflected by the microsphere suspension in each interval at height j is detected by the data processing component 2. By integrating using the above formula, the total reflected signal of the microspheres in the entire suspension can be obtained.
[0030] In this embodiment, the data processing component 2 is also used to integrate the relative signal intensity returned by microspheres at different heights in the microsphere suspension within a range above a preset height to obtain the cumulative signal quantity.
[0031] Simultaneously, according to the testing requirements, theoretically, it is necessary to detect the time required for the microsphere suspension to separate and reach 2 / 3 of the container height (i.e., the 10mL mark). Therefore, the cumulative signal returned by the microsphere suspension above 2 / 3 of the container height can be detected. This can be calculated using the following formula:
[0032] in, I(x) represents the cumulative signal intensity obtained by integrating within the range above a preset height h, where h represents the preset height, x∈[0,h], and I(x) represents the relative signal intensity of different liquid level intervals x. According to the testing principle, h=15×1 / 3=5. Taking the initial time as the shaking and placement of the microsphere suspension, as the microspheres continuously settle, it is evident that the number of microspheres above 2 / 3 of the container height decreases. Therefore, the actual detected cumulative signal intensity S2 decreases dynamically until the microsphere suspension stratifies to 2 / 3 of the container height. At this point, the cumulative signal intensity is detected. When the signal receiver 4 receives the returned cumulative signal intensity S2, which continues to decrease, the ratio of the cumulative signal intensity S2 to the total reflected signal intensity S1 continuously changes until this ratio (i.e., the real-time detection value) is equal to or less than the detection threshold. At this point, the data output component can determine that the microsphere suspension has stratified to 2 / 3 of the container height or below. The corresponding time is the suspension time for detecting the microsphere's suspendability. As an example, after the test begins, the laser emitter 3 continuously emits pulse signals, and the data processing component 2 continuously monitors the S2 / S1 value at intervals of approximately 1 second. The ratio of the accumulated signal quantity S2 to the total reflected signal quantity S1, i.e. The ratio of the two values is used as the real-time detection value. The test is terminated when the set detection threshold is reached or the value is less than the detection threshold, and the microsphere suspension time t is output.
[0033] Please see again Figure 2 The detection module 81 further includes an upper base 1 and a housing 8. The upper base 1 is used to ensure stable placement when the microsphere levitation detection device is inverted. The housing 8 is a cover for the detection module 81. The data processing component 2 is electrically connected to the laser emitter 3 and the signal receiver 4, respectively. The data processing component 2, the laser emitter 3, and the signal receiver 4 are disposed within the cavity of the housing 8.
[0034] The detection module 81 also includes a timing module ( Figure 2 (Not shown), the timing module is connected to the data processing component 2, and the timing module includes a start button located on the housing 8. Figure 2 (not shown) and display screen ( Figure 2(Not shown); the start button is used to start timing when initiating the microsphere levitation test. The display screen is used to display the levitation time and real-time detection value after the microsphere levitation test is completed. The levitation time can be displayed using digital time.
[0035] Please see again Figure 2 The sampling module 82 also includes a lower base 9, a buffer chamber 6, and a liquid inlet / outlet 7. The test container 5 is disposed above the lower base 9. One end of the buffer chamber 6 is connected to the test container 5, and the other end of the buffer chamber 6 is provided with the liquid inlet / outlet 7. The buffer chamber 6 is used to retain air bubbles.
[0036] The microsphere suspension detection device provided in this embodiment can accurately detect the suspension time of microspheres, avoid subjective judgment by operators, reduce detection errors of suspension time, and improve detection efficiency.
[0037] This application provides a method for detecting the suspension of microspheres, using the microsphere suspension detection device provided in this application. The method includes steps S1-S6, which are described below.
[0038] like Figure 3 As shown, the method for detecting the suspension of microspheres includes: S1; After determining that the microsphere suspension to be tested has the property of downward sedimentation, place the microsphere suspension detection device upright, so that the detection module of the microsphere suspension detection device is located on the upper side of the sampling module, where the upper side is opposite to the direction of gravity.
[0039] Please see again Figure 2 , Figure 2 The detection module 81 shown is located above the layout module 82 and is placed facing forward.
[0040] S2: Input the preset detection threshold into the data output component of the microsphere suspension detection device.
[0041] In this embodiment, obtaining the detection threshold includes: Receive an empirically preset threshold input by the user, and determine the preset threshold as the detection threshold; or... Select a standard microsphere suspension and place it into the test container; When the layered interface in the test container is determined to be at a preset height, the total standard reflected signal returned by microspheres at different heights in the standard microsphere suspension and the standard cumulative signal returned by microspheres above the preset height in the standard microsphere suspension are obtained. The ratio of the standard cumulative signal quantity to the total standard reflected signal quantity is determined as the detection threshold.
[0042] In this embodiment, the type of microspheres to be tested was determined, and 16 mL of a standard microsphere suspension containing microspheres and contrast agent was prepared according to the required ratio. Then, as follows... Figure 4 Place the device on its side and add the standard microsphere suspension 21 into the 15 mL test container 5 through the liquid inlet / outlet 7. Continue until... Figure 5 The standard microsphere suspension is submerged in the buffer chamber 6, and the liquid inlet and outlet 7 are closed. The device is then erected, as shown. Figure 6 Ensure that bubble 22 remains within buffer chamber 6, while test container 5 is free of bubbles. Then press the start button.
[0043] After startup, data processing component 2 can determine the relative signal of the microsphere at different liquid levels based on the time from the emission of the pulse signal from laser emitter 3 to the reception time of the reflected signal from signal receiver 4, and the corresponding signal intensity. It then integrates the total signal returned by the microsphere at different heights to calculate the total standard reflected signal S3 returned by a single pulse, and the standard cumulative signal S4 returned by the microsphere above 2 / 3 of the container height. The instrument continuously calculates the ratio of S4 / S3, allowing it to stand and be visually observed until the microsphere settles to the 10mL mark on the container. The calculated S4 / S3 ratio is recorded as N, and N is input into data processing component 2 as the detection threshold.
[0044] Furthermore, since the sedimentation time of microspheres depends on the operator's judgment, a fixed detection threshold, such as 5%, can be set directly based on historical experience. Theoretically, this will not affect the parallelism and accuracy of the experimental results.
[0045] S3: After placing the well-shaken microsphere suspension into the test container of the microsphere suspension detection device, immediately press the start button on the microsphere suspension detection device, and the display screen of the microsphere suspension detection device will start timing.
[0046] In this embodiment, the test container of the microsphere suspension detection device is first emptied, and then the microsphere suspension to be tested is placed into the test container 5 and shaken well, ensuring that there are no air bubbles in the test container 5. The operation method can be referred to Figures 4-6 After shaking the test container 5 thoroughly, press the start button, and the display will start timing.
[0047] S4: The laser emitter of the microsphere suspension detection device emits a pulse signal to the microsphere suspension, and the signal receiver of the microsphere suspension detection device receives the reflected signal obtained by the microspheres in the microsphere suspension reflecting the pulse signal.
[0048] S5: The data processing component of the microsphere suspension detection device obtains real-time detection values based on the emission time of the pulse signal, the reception time of the reflected signal, and the signal strength of the reflected signal.
[0049] In this embodiment, S5 includes: using the data processing component to acquire in real time the cumulative signal amount returned by the microspheres above a preset height and the total reflected signal amount returned by the microspheres throughout the entire height range, based on the transmission time, the reception time, and the signal strength of the reflected signal, and using the ratio of the cumulative signal amount to the total reflected signal amount as the real-time detection value.
[0050] In this embodiment, obtaining the total reflected signal returned by the microspheres throughout the entire height range of the microsphere suspension includes: The total reflected signal quantity is obtained by integrating the relative signal intensity returned by microspheres at different heights in the microsphere suspension over the entire height range.
[0051] In this embodiment, acquiring the cumulative signal returned by the microspheres in the microsphere suspension at a preset height includes: The cumulative signal quantity is obtained by integrating the relative signal intensity returned by microspheres at different heights in the microsphere suspension within a range above a preset height.
[0052] In this embodiment, obtaining the relative signal intensity returned by microspheres at different heights in the microsphere suspension includes: The relative signal strength returned by microspheres at different heights in the microsphere suspension is determined based on the emission time, the reception time, and the signal strength of the reflected signal.
[0053] In this embodiment, the total reflected signal quantity is denoted as S1 and the cumulative signal quantity is denoted as S2. The relevant process for calculating the total reflected signal quantity S1 and the cumulative signal quantity S2 can be found in the aforementioned description of the detection device. To avoid repetition, it will not be repeated here.
[0054] In this embodiment, after the test begins, the data processing component 2 continuously measures the total reflected signal S1 and the cumulative signal S2, and calculates the ratio of S2 / S1, with each calculation approximately every 1 second.
[0055] S6: The data output component of the microsphere suspension detection device determines whether the real-time detection value is less than or equal to the detection threshold. If so, the display screen stops timing, and the current time displayed on the display screen is the suspension time of the microsphere suspension.
[0056] At time 0, when the microspheres are evenly dispersed and timing begins, the state of the microspheres in the microsphere suspension detection device is as follows: Figure 7 The corresponding total reflected signal S-curve diagram is shown below. Figure 8As shown, the microspheres are uniformly dispersed, and the concentration of microspheres is approximately the same at each depth in the suspension, so the amount of signal returned at each depth is also approximately the same. Therefore, as the distance increases, the total amount of signal returned within that distance also increases uniformly, and the curve on the coordinate graph is close to an oblique line.
[0057] As the microspheres gradually settle, such as Figure 9 As shown, the concentration of microspheres increases from top to bottom. Initially, with increasing distance, the concentration of microspheres is lower at the top of the suspension, resulting in a slow increase in the total reflected signal (S-curve) as the distance increases. However, after reaching a certain distance, the concentration of microspheres gradually increases, leading to a significant increase in the returned signal, thus forming... Figure 10 The S-shaped curve of the total reflected signal is shown.
[0058] As the microspheres settle, the total reflected signal remains basically unchanged. However, the cumulative signal S2 above the 10mL mark will continue to decrease as the number of microspheres above the 10mL mark decreases. If detection continues after reaching the detection threshold, the real-time detected value will eventually approach 0.
[0059] As the accumulated signal quantity S2 gradually decreases, the value of S2 / S1 also decreases over time. When the S2 / S1 output by data processing component 2 is less than or equal to the preset detection threshold N, it proves that the sedimentation degree of the microspheres has reached the preset requirement. This part is automatically calculated and compared by data processing component 2, and its execution logic is as follows: Figure 11 As shown, the process includes the following steps: S10, start timing. S11, wait 1 second, calculate S2 / S1. S12, determine if S2 / S1 is less than or equal to the detection threshold N. If yes, proceed to S13; otherwise, proceed to S12. S13, output the float time and stop timing. After timing stops, record the time from the start of timing to the stop of timing; this is the float time of the microsphere.
[0060] In this embodiment, the method further includes: If the microspheres in the microsphere suspension have floating characteristics, the microsphere suspension detection device is inverted so that the detection module is located below the sampling module, wherein the lower side is in the same direction as gravity.
[0061] It is understandable that, due to differences in the material and density of the microspheres, some microspheres will not sink after being mixed with the contrast agent, but will gradually float. The suspension time of these microspheres is the time from their uniform distribution to when they float to more than 1 / 3 of the test container 5, i.e., above the 5mL mark. For testing these types of microspheres, simply invert the device so that the upper base 1 is at the bottom of the device. Other testing methods are the same as those used to test the suspension time of microspheres with sinking characteristics, and will not be elaborated here.
[0062] The implementation schemes of this application will be described in detail below with reference to specific embodiments. However, those skilled in the art will understand that the following embodiments are only for illustrating this application and should not be regarded as limiting the scope of this application. Unless otherwise specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall apply. Reagents or instruments used without specified manufacturers are all conventional products that can be purchased commercially.
[0063] Example The following describes the testing of a commercially available microsphere using this microsphere suspension detection method. The particle size was selected as 500-700 μm. 2 mL of microspheres were taken and mixed with 6 mL of physiological saline in a syringe. Then, 8 mL of iohexol 300 was added at a 1:1 ratio and mixed thoroughly to obtain 16 mL of a homogeneous microsphere suspension. This microsphere suspension was injected into test container 5, and the microsphere suspension detection device was then held vertically to ensure that there were no air bubbles in test container 5.
[0064] Then, press the start button on the microsphere suspension detection device to enter the pre-experiment mode. At this time, the display shows the real-time detection value with the ratio of S4 / S3. When the microspheres are observed to have settled to 2 / 3 of the scale mark in the container, record the real-time detection value of 7% displayed on the display as the detection threshold, and then input this detection threshold of 7% into the data output component.
[0065] Prepare the microsphere suspension using five different batches of the same type and specifications, as described above: 2 mL microspheres + 6 mL physiological saline + 8 mL iohexol 300. Add the microsphere suspension to test container 5, ensuring the container is free of air bubbles. Press the start button to enter test mode; the device display will show the time. Press start again and allow the microspheres to settle.
[0066] When S2 / S1 reaches the detection threshold, the device stops timing and emits a prompt sound, recording the hover time displayed on the screen at this time.
[0067] The above experiment was repeated five times, and the suspension time was recorded. The results are as follows;
[0068] The results showed that the microspheres from different batches all exhibited good suspension performance with an error within 5%, which met expectations, and the test was completed.
[0069] The microsphere suspension detection method provided in this embodiment can accurately detect the suspension time of microspheres, avoid subjective judgment by operators, reduce detection errors of suspension time, and improve detection efficiency.
[0070] It should be noted that, in this document, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or terminal that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or terminal. Unless otherwise specified, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or terminal that includes that element.
[0071] The embodiments of this application have been described above with reference to the accompanying drawings. However, this application is not limited to the specific embodiments described above. The specific embodiments described above are merely illustrative and not restrictive. Those skilled in the art can make many other forms under the guidance of this application without departing from the spirit and scope of the claims, and all of these forms are within the protection scope of this application.
Claims
1. A microsphere suspension detection device, characterized in that, include: Lofting module and detection module; The sampling module includes a test container, which is used to hold the microsphere suspension. The detection module includes a laser emitter, a signal receiver, a data processing component, and a data output component; The laser emitter is used to emit pulse signals to the microsphere suspension in the test container and to send the emission time of the pulse signals to the data processing component; The signal receiver is used to receive the reflected signal obtained by the microspheres in the microsphere suspension reflecting the pulse signal, and to send the reception time of the reflected signal to the data processing component; The data processing component is used to obtain the cumulative signal amount returned by the microspheres above a preset height and the total reflected signal amount returned by the microspheres in the entire height range based on the transmission time, the reception time, and the signal strength of the reflected signal, and to send the ratio of the cumulative signal amount to the total reflected signal amount as a real-time detection value to the data output component. The data output component is used to obtain the hover time based on the real-time detection value and the preset detection threshold.
2. The detection device according to claim 1, characterized in that, The data output component is also used to determine whether the real-time detection value is less than or equal to the detection threshold. If so, the hover time is determined based on the initial time of starting the test and the current time.
3. The detection device according to claim 2, characterized in that, The detection module also includes an upper base and a housing, and the data processing component is electrically connected to the laser emitter and the signal receiver respectively; The detection module also includes a timing module, which includes a start button and a display screen disposed on the housing. The start button is used to start the timer when the microsphere suspension test is initiated; The display screen is used to display the suspension time and the real-time detection value after the microsphere suspension test is completed.
4. The apparatus according to claim 3, characterized in that, The sampling module also includes a lower base, a buffer chamber, and a liquid inlet and outlet. The test container is disposed above the lower base. One end of the buffer chamber is connected to the test container, and the other end of the buffer chamber is provided with the liquid inlet and outlet. The buffer chamber is used to retain air bubbles.
5. A method for detecting the suspension of microspheres, characterized in that, The method, applied to the microsphere suspension detection device according to claim 3 or 4, comprises: S1; After determining that the microsphere suspension to be tested has the property of downward sedimentation, the microsphere suspension detection device is placed upright, so that the detection module of the microsphere suspension detection device is located on the upper side of the sampling module, wherein the upper side is opposite to the direction of gravity. S2: Input the preset detection threshold into the data output component of the microsphere suspension detection device; S3: After placing the well-shaken microsphere suspension into the test container of the microsphere suspension detection device, immediately press the start button in the microsphere suspension detection device, and the display screen of the microsphere suspension detection device will start timing. S4: The laser emitter of the microsphere suspension detection device emits a pulse signal to the microsphere suspension, and the signal receiver of the microsphere suspension detection device receives the reflected signal obtained by the microspheres in the microsphere suspension reflecting the pulse signal; S5: The data processing component of the microsphere suspension detection device obtains real-time detection values based on the emission time of the pulse signal, the reception time of the reflected signal, and the signal strength of the reflected signal; S6: The data output component of the microsphere suspension detection device determines whether the real-time detection value is less than or equal to the detection threshold. If so, the display screen stops timing, and the current time displayed on the display screen is the suspension time of the microsphere suspension.
6. The method according to claim 5, characterized in that, Obtaining the detection threshold includes: Receive an empirically preset threshold input by the user, and determine the preset threshold as the detection threshold; or... Select a standard microsphere suspension and place the standard microsphere suspension into the test container; When the layered interface in the test container is determined to be at a preset height, the total standard reflected signal returned by microspheres at different heights in the standard microsphere suspension and the standard cumulative signal returned by microspheres above the preset height in the standard microsphere suspension are obtained. The ratio of the standard cumulative signal quantity to the total standard reflected signal quantity is determined as the detection threshold.
7. The method according to claim 5, characterized in that, The data processing component of the microsphere levitation detection device acquires real-time detection values based on the emission time of the pulse signal, the reception time of the reflected signal, and the signal strength of the reflected signal, including: The data processing component acquires in real time the cumulative signal amount returned by the microspheres above a preset height and the total reflected signal amount returned by the microspheres throughout the entire height range, based on the transmission time, the reception time, and the signal strength of the reflected signal. The ratio of the cumulative signal amount to the total reflected signal amount is used as the real-time detection value.
8. The method according to claim 7, characterized in that, Obtaining the total reflected signal returned by the microspheres in the microsphere suspension over the entire height range includes: The total reflected signal quantity is obtained by integrating the relative signal intensity returned by microspheres at different heights in the microsphere suspension over the entire height range. Acquiring the cumulative signal returned by the microspheres in the microsphere suspension above a preset height includes: The cumulative signal quantity is obtained by integrating the relative signal intensity returned by microspheres at different heights in the microsphere suspension within a range above a preset height.
9. The method according to claim 8, characterized in that, Obtaining the relative signal intensity returned by microspheres at different heights in the microsphere suspension includes: The relative signal strength returned by microspheres at different heights in the microsphere suspension is determined based on the emission time, the reception time, and the signal strength of the reflected signal.
10. The method according to claim 5, characterized in that, The method further includes: If the microspheres in the microsphere suspension have floating characteristics, the microsphere suspension detection device is inverted so that the detection module is located below the sampling module, wherein the lower side is in the same direction as gravity.