Portable collection device and method for human exhaled air condensate of double-circulation liquid cooling system

Abstract

The invention relates to the technical field of medical instruments, in particular to a portable collection device and method for human exhaled air condensate of a double-circulation liquid cooling system.The portable collection device comprises a host, a handheld collector and a flexible connection system, and the flexible connection system is connected between the host and the handheld collector; the main machine comprises a first refrigerating system and a cooling liquid circulating system, the first refrigerating system is used for conducting primary cooling on cooling liquid, and the cooling liquid circulating system is used for driving the cooling liquid to circulate between the first refrigerating system and the handheld collector; the handheld collector comprises a heat preservation shell, a second refrigerating system and a liquid cooling radiator are arranged in the heat preservation shell, the second refrigerating system and the liquid cooling radiator are arranged in a heat conduction mode, and the liquid cooling radiator is communicated with the first refrigerating system through a flexible connection system. The EBC non-invasive diagnosis system has more excellent effects in portability, profound hypothermia performance, collection efficiency, sample fidelity and operation experience, and the clinical practical process of the EBC non-invasive diagnosis technology is powerfully promoted.

Description

Technical Field

[0001] This invention relates to the field of medical device technology, and in particular to a portable device and method for collecting condensate from human exhaled breath using a dual-circulation liquid cooling system. Background Technology

[0002] Exhaled breath condensate (EBC) testing is medically renowned as a "biochemical lung function" test, directly reflecting changes in lung biochemistry. EBC sampling is an important non-invasive testing method. However, existing condensate sampling devices face an irreconcilable conflict between performance and portability, specifically manifested in the following ways: The contradiction between cooling efficiency and portability: Although devices using compressor cooling have high cooling power and good low-temperature stability, they are bulky and cannot achieve convenient bedside diagnosis and mobile applications; while portable devices using semiconductor cooling have limited cooling efficiency and poor heat dissipation, making it difficult to achieve the rapid cooling and deep low temperature required for condensation. Especially when long-term stable sampling is required, large temperature fluctuations result in low condensation efficiency and poor sample representativeness.

[0003] Issues with condensation collection efficiency and sample fidelity: Traditional condenser surfaces have poor hydrophobicity, easily forming liquid films that hinder efficient condensation and droplet detachment. Furthermore, the surface properties may adsorb target analytes such as proteins and viruses from the sample, leading to sample loss and distorted test results. Additionally, slow cooling, high temperatures, and long sampling times result in the easy degradation of unstable biomarkers in the sample. Existing equipment struggles to achieve deep cryogenic temperatures below -20°C, limiting its efficient capture of ultravolatile substances and sample fidelity.

[0004] In summary, current technologies lack an EBC acquisition device that can meet both clinical portability needs and provide research-grade deep cryogenic and high-efficiency acquisition performance.

[0005] Therefore, the present invention provides a portable device and method for collecting human exhaled breath condensate from a dual-cycle liquid cooling system to solve the above-mentioned technical problems. Summary of the Invention

[0006] The purpose of this invention is to provide a portable device and method for collecting condensate from human exhaled breath in a dual-cycle liquid cooling system, in order to solve the problems existing in the prior art.

[0007] To achieve the above objectives, the present invention provides the following solution: The present invention provides a portable collection device for human exhaled gas condensate in a dual-circulation liquid cooling system, comprising a main unit, a handheld collector, and a flexible connection system, wherein the flexible connection system is connected between the main unit and the handheld collector; The host includes a first refrigeration system and a coolant circulation system. The first refrigeration system is used to perform primary cooling of the coolant, and the coolant circulation system is used to drive the coolant to circulate between the first refrigeration system and the handheld collector. The handheld data collector includes an insulated outer shell, inside which a second refrigeration system and a liquid-cooled radiator are installed. The second refrigeration system and the liquid-cooled radiator are connected by heat conduction. The second refrigeration system is equipped with a sampling component for sampling. The liquid-cooled radiator is connected to the first refrigeration system through the flexible connection system.

[0008] Preferably, the first refrigeration system includes a first liquid storage chamber for storing coolant, which is circulated in connection with the coolant circulation system; the first liquid storage chamber is provided with a spirally arranged evaporator, which is connected in series with a compressor, a condenser and a throttling device to form a vapor compression refrigeration circuit to cool the coolant in the first liquid storage chamber.

[0009] Preferably, the first refrigeration system includes at least two sets of cooling modules, and the multiple sets of cooling modules are connected in parallel through valve group modules; the cooling modules are connected to the coolant circulation system to realize pre-cooling and cooling synergy among the cooling modules.

[0010] Preferably, the cooling module includes a semiconductor refrigeration chip assembly, with a hot-end heat sink attached to the hot end of the semiconductor refrigeration chip assembly, and a cold-end liquid cooling plate attached to the cold end of the semiconductor refrigeration chip assembly. The outlet of the cold-end liquid cooling plate is connected to the coolant circulation system, and the inlet of the cold-end liquid cooling plate is connected to the valve assembly module.

[0011] Preferably, the coolant circulation system includes a second reservoir, the outlet of which is connected to a liquid pump, and the outlet of the liquid pump is quickly connected to the flexible connection system via a pipeline system.

[0012] Preferably, the heat-insulating shell is provided with a condensation chamber with a U-shaped groove structure, which has a larger condensation surface area and constrains and accelerates the airflow; several temperature sensors are installed on the back of the condensation chamber.

[0013] Preferably, the second refrigeration system includes several sets of semiconductor refrigeration chips, the cold end of the semiconductor refrigeration chip is attached to the back of the outer wall of the condensation cavity, and the hot end plane of the semiconductor refrigeration chip is attached to the surface of the liquid-cooled heat sink.

[0014] Preferably, the sampling assembly includes a condenser tube that passes through the condensation chamber and is press-fitted against the side wall of the condensation chamber; the bottom end of the condenser tube is connected to a condensate collection tube detachably connected to the bottom end of the handheld sampler.

[0015] Preferably, the flexible connection system includes two parallel and independent coolant connecting pipes, the outer wall of which is fitted with a stainless steel braided mesh; the two coolant connecting pipes are wrapped together by an insulation layer.

[0016] This invention also discloses a method for collecting human exhaled condensate using a portable collection device based on a dual-circulation liquid cooling system, comprising the following steps: Connect and initialize the device, then start the first refrigeration system to pre-cool the coolant to the set temperature, and enter the standby ready state; The equipment is started, and the second cooling system cools the working area of ​​the handheld data collector to the set temperature. The second cooling system switches its working mode to maintain the temperature stability of the handheld sampler, and then performs exhalation sampling. The collected samples condense into liquid and collect. After the preset sampling time or data collection time is reached, the second cooling system shuts down or reduces its power, but the first cooling system continues to run for a period of time. The first refrigeration system was shut down, and the sample was taken for analysis.

[0017] Compared with existing technologies, this invention has the following advantages and technical effects: This invention discloses a portable collection device and method for human exhaled gas condensate using a dual-cycle liquid cooling system. It completely abandons the traditional integrated design, adopting a split architecture consisting of an independent main unit, a handheld collector, and a flexible connection system connecting the two. This resolves the long-standing contradiction in the field of refrigeration equipment between performance and portability. The handheld collector allows for single-handed, flexible operation for bedside and mobile sampling; while the main unit integrates high-power, high-efficiency cooling and heat dissipation modules without sacrificing performance for portability. The core of this design lies in functional decoupling and energy transfer. Through the flexible connection system, the powerful cooling and power supply functions of the main unit are physically separated from the condensation sampling function of the handheld collector, yet functionally tightly coupled. This allows the lightweight handheld collector to continuously receive cooling support at the level of large equipment, representing a fundamental innovation at the system level. The efficient coupling between the first cooling system within the main unit and the second cooling system located within the handheld collector results in a qualitative leap in the cooling performance of the handheld collector.

[0018] This invention, through an innovative architecture that combines a split-type flexible connection, a dual cooling system, and circulating liquid cooling, achieves superior performance in terms of portability, deep cryogenic performance, acquisition efficiency, sample fidelity, and user experience, thus powerfully promoting the clinical application of EBC non-invasive diagnostic technology. Attached Figure Description

[0019] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly described below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort. In the drawings: Figure 1 This is a schematic diagram of a portable collection device for human exhaled breath condensate from a dual-circulation liquid cooling system according to the present invention. Figure 2 This is a schematic diagram of the first cooling system of the present invention; Figure 3 This is a schematic diagram of the handheld data collector of the present invention; Figure 4 This is a cross-sectional view of the handheld data collector of the present invention; Figure 5 This is a longitudinal sectional view of the handheld data collector of the present invention; Figure 6 This is a schematic diagram of the condenser tube of the present invention; Figure 7 This is a schematic diagram of the condenser tube installation of the present invention; Figure 8 This is a schematic diagram of the internal structure of the handheld data collector of the present invention; Figure 9 This is a schematic diagram of the flexible connection system structure of the present invention; Figure 10 This is a flowchart of the inventor's method for collecting exhaled breath condensate; Figure 11 This is a schematic diagram of the first cooling system in Embodiment 2 of the present invention; Figure 12 This is a temperature change diagram of a specific example 1 of the present invention; Figure 13 This is a temperature change graph for a specific example of the present invention, 2. Figure 14 This is a temperature change diagram for specific example 3 of the present invention; Figure 15 This is a temperature change diagram for specific example 4 of the present invention; Figure 16 This is a temperature change diagram for specific example 5 of the present invention; Figure 17 This is a temperature change diagram for specific example 6 of the present invention; In the diagram: 1. Main unit; 2. Handheld data acquisition unit; 3. Flexible connection system; 1-1. Display screen; 1-2. Function buttons; 1-3. Quick interface; 1-4. Power interface; 2-1. Data acquisition unit interface; 2A-1. Compressor; 2A-2. Condenser; 2A-3. Evaporator; 2A-4. First liquid storage chamber; 2A-5. Throttling device; 2B-1. Semiconductor cooling chip assembly; 2B-2. Cold end liquid cooling plate; 2B-3. Hot end radiator; 2B-4. Cooling fan; 2B-5. Valve module; 2B -6. Second liquid storage chamber; 2B-7. Liquid pump; 3-1. Condensation chamber; 3-2. Semiconductor cooling chip; 3-3. Liquid cooling radiator; 3-4. Insulation shell; 3-5. Series pipe; 3-6. Coolant flow channel; 3-7. External pipe; 4-1. Condensation pipe; 4-2. U-shaped groove longitudinal opening; 4-3. Magnetic retaining ring; 4-4. Bottom fixing piece; 4-5. Collection pipe; 5-1. Coolant connecting pipe; 5-2. Stainless steel braided mesh; 5-3. Insulation layer; 5-4. Power cord; 5-5. Shielded signal cable. Detailed Implementation

[0020] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0021] Exhaled breath condensate (EBC) is a biological sample obtained by cooling exhaled air through the mouth and nose and condensing it in vitro. This sample contains a mixture of water vapor, small particles or droplets formed by the atomization of airway lining fluid, and water-soluble volatiles from the exhaled air. It includes polar electrolytes, amino acids, cytokines, chemokines, peptides, small proteins, metabolites, nucleic acids, and various soluble small molecules such as lipids / arachidic acids. These substances enter the exhaled air after gas exchange with the blood at the pulmonary capillary interface, providing valuable omics-level information for assessing individual health status. Due to the significant advantages of EBC collection—non-invasiveness, simplicity, and high reproducibility—it shows great clinical application potential in areas such as respiratory disease screening, monitoring of inflammation and oxidative stress, efficacy evaluation, and large-scale epidemiological surveys.

[0022] However, the clinical translation of EBC technology faces two major bottlenecks: first, the concentration of the target biomarker in the sample is extremely low (typically in the nanogram to picogram per milliliter range); second, the condensation efficiency of existing collection devices is generally low, with collection rates mostly between 50 and 200 microliters per minute. This means that to obtain a sufficient sample volume for accurate analysis (e.g., 500-1000 microliters), the collection time can be as long as 5 to 10 minutes. This "double low" situation of "low concentration" and "low efficiency" severely restricts the practicality and promotion of EBC in clinical settings.

[0023] Ultimately, the bottlenecks mentioned above stem from the following inherent flaws in the core structural design of existing data acquisition devices: The cooling structure is rudimentary: In integrated devices, the hot end of the semiconductor cooling chip relies solely on a small air-cooled heat sink, which has low heat dissipation efficiency and severely restricts the cooling capacity of the cold end, making it impossible to achieve rapid cooling and maintain a stable deep low temperature.

[0024] Currently, common EBC acquisition devices mainly employ two types of cooling structures: Currently, EBC acquisition devices on the market and reported in literature can be mainly divided into two categories, and their structural characteristics and corresponding disadvantages are described below: 1. Passive pre-cooling and condensing unit These devices typically consist of a metal (such as aluminum or stainless steel) or plastic (such as polypropylene) condenser tube with a built-in cavity, and are generally cylindrical or simply curved tubular in shape. Before use, the entire device needs to be pre-cooled in a refrigerator (e.g., -20°C) or its outer sleeve should be filled with ice packs or an ice-water mixture as a cold source. During actual sampling, the condenser tube acts as an isolated "cold storage body," with limited initial cooling capacity and no continuous replenishment of cooling. As sampling progresses, the heat from exhaled breath is continuously absorbed by the tube wall, causing the condenser tube temperature to rise rapidly, making it impossible to maintain a stable low-temperature environment. The disadvantages resulting from this structure are: The temperature is uncontrollable and continues to rise: there is no active cooling structure, the cooling capacity is constantly consumed, and the condensation efficiency drops sharply as the sampling time increases; Unable to achieve deep low temperatures: It relies on external pre-cooling, and the lowest temperature is limited by the capacity of the pre-cooling equipment (such as a refrigerator), and it is difficult to maintain below -15°C to -20°C in actual operation; Low condensation surface efficiency: The airflow in a straight cylindrical pipe tends to form laminar flow, with a small contact area and short contact time with the pipe wall, resulting in a large amount of water vapor being discharged before it can be fully condensed.

[0025] 2. Active semiconductor cooling integrated device These devices integrate a thermoelectric cooler (TEC) into a handheld or desktop housing to directly cool the condenser chamber. A typical structure includes an aluminum alloy or stainless steel condenser chamber (usually cylindrical or rectangular), with the TEC attached to the back of the chamber using thermally conductive adhesive. The hot end of the TEC is connected to a small finned aluminum heat sink, and a miniature fan provides forced air cooling.

[0026] This structure has the following significant drawbacks: Poor heat dissipation efficiency: In handheld or small desktop devices, heat dissipation space is extremely limited. Small finned heat sinks have insufficient surface area, and fans have low airflow, resulting in the inability to dissipate waste heat from the hot end of the thermoelectric cooler in a timely manner, causing the hot end temperature to remain high. According to the Peltier effect, this severely limits the cooling capacity and minimum achievable temperature of the cold end (typically only maintaining a range of -5°C to 0°C). Large temperature fluctuations: Air cooling is significantly affected by ambient temperature, and the start and stop of the fan can easily cause periodic temperature fluctuations, which is not conducive to stable condensation. The condensation chamber structure is not optimized: most still use a straight cylindrical cavity, and the inner wall is mostly made of hydrophilic metal or plastic. The liquid film formed by condensation has strong adhesion and is not easy to fall off, which creates thermal resistance and further reduces condensation efficiency. The contradiction between size and performance: If you try to improve performance by increasing the number of cooling chips or enlarging the heat sink, the size, weight and noise of the device will increase significantly, and portability will be lost.

[0027] 3. High-performance integrated compressor refrigeration units (such as some research-grade equipment) These devices employ a small vapor compression refrigeration cycle (including a compressor, condenser, expansion valve, and evaporator), with the evaporator directly attached to the condenser chamber, achieving temperatures as low as -20°C or even lower. However, they are bulky (typically benchtop models, weighing >10kg), noisy (due to compressor operation), and vibrations can affect sample collection. While they solve the low-temperature problem, they lack portability and are unsuitable for bedside diagnostics, home healthcare, or mobile medical settings.

[0028] The core contradictions of existing technological structural defects can be summarized as follows: 1. Portable but poor performance (passive or semiconductor refrigeration integrated unit), or high performance but bulky (compressor refrigeration integrated unit), unable to achieve a balance between portability, deep low temperature, high efficiency, and high stability; 2. The condensation chamber and flow channel design is rudimentary and not optimized from the perspective of fluid mechanics and heat transfer, resulting in low gas-liquid contact efficiency and condensate retention.

[0029] 3. Lack of systematic structural consideration for sample fidelity; the condensation surface material is prone to adsorbing biomolecules, and unstable temperature may lead to sample degradation.

[0030] The fundamental reason why existing EBC collection devices cannot meet the needs of rapid clinical diagnosis and high-throughput sampling is that their limitations in portability, cooling capacity, temperature stability, and condensation efficiency are insurmountable.

[0031] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0032] Example 1 Reference Figures 1 to 10 As shown, this embodiment provides a portable collection device for human exhaled condensate from a dual-cycle liquid cooling system, including a main unit 1, a handheld collector 2, and a flexible connection system 3, with the flexible connection system 3 connecting the main unit 1 and the handheld collector 2. The host 1 includes a first refrigeration system and a coolant circulation system. The first refrigeration system is used for primary cooling of the coolant, and the coolant circulation system is used to drive the coolant to circulate between the first refrigeration system and the handheld collector 2. The handheld collector 2 includes an insulated shell 3-4, inside which a second refrigeration system and a liquid-cooled radiator 3-3 are installed. The second refrigeration system and the liquid-cooled radiator 3-3 are connected by heat conduction. The second refrigeration system is equipped with a sampling component for sampling. The liquid-cooled radiator 3-3 is connected to the first refrigeration system through a flexible connection system 3.

[0033] This invention discloses a portable device and method for collecting human exhaled breath condensate using a dual-cycle liquid cooling system. It completely abandons the traditional integrated design, adopting a split architecture consisting of an independent main unit 1, a handheld collector 2, and a flexible connection system 3 connecting the two. This resolves the long-standing contradiction in the field of refrigeration equipment between performance and portability. The handheld collector 2 allows for one-handed, flexible operation for bedside and mobile sampling; while the main unit 1 integrates high-power, high-efficiency cooling and heat dissipation modules without sacrificing performance for portability. The core of this design lies in functional decoupling and energy transfer. Through the flexible connection system 3, the powerful cooling and power supply functions of the main unit 1 are physically separated from the condensation sampling function of the handheld collector 2, yet functionally tightly coupled. This allows the lightweight handheld collector 2 to continuously receive cooling support at the level of large equipment, representing a fundamental innovation at the system level. The efficient coupling between the first cooling system within the main unit 1 and the second cooling system within the handheld collector 2 results in a qualitative leap in the cooling performance of the handheld collector 2. This invention, through an innovative architecture that combines a split-type flexible connection, a dual cooling system, and circulating liquid cooling, achieves superior performance in terms of portability, deep cryogenic performance, acquisition efficiency, sample fidelity, and user experience, thus powerfully promoting the clinical application of EBC non-invasive diagnostic technology.

[0034] In one embodiment of the present invention, the main unit 1 provides power, core cooling, and control functions to the main system. Its outer casing is a vertical or horizontal box, which houses the main components of the first cooling system and the coolant circulation system. The front panel of the main unit 1 is provided with a human-machine interface, including a display screen 1-1, function buttons 1-2, status indicator lights, and a quick interface 1-3 for connecting to the flexible connection system 3; the rear panel of the main unit 1 is provided with a power interface 1-4.

[0035] In one embodiment of the present invention, the handheld sampler 2 is a user-operated component responsible for the introduction, condensation, and sample collection of exhaled air. Its housing is ergonomically designed, lightweight, and made of a non-slip material. The housing integrates core cold-end components such as a condensation chamber 3-1, a second refrigeration system, and a liquid-cooled radiator 3-3. An insulation layer 5-3 is provided on the inner side to reduce cold loss.

[0036] In one embodiment of the present invention, the handheld collector 2 weighs approximately 300-600g.

[0037] In one embodiment of the present invention, the insulation layer 5-3 on the inner wall of the shell can be selected as 5mm thick foamed polyethylene.

[0038] In one embodiment of the present invention, the flexible connection system 3 is a connection structure that connects the host 1 and the handheld collector 2, and can transmit coolant, power and control signals through a physical interface.

[0039] In one embodiment of the present invention, the core of the split flexible connection architecture lies in "functional decoupling and energy transfer". Through the flexible connection system 3, the "powerful cooling and power supply" function of the host 1 and the "condensation sampling" function of the handheld collector 2 are physically separated, but functionally tightly coupled, which is like establishing a continuous "energy conveyor belt" and "heat dissipation channel" for the handheld collector 2, so that the lightweight collector can continuously obtain cooling support at the level of large equipment.

[0040] In one embodiment of the present invention, the principle and process of coordinated heat dissipation by a first refrigeration system and a second refrigeration system are adopted: Primary cooling: The coolant flows through the cold end of the first refrigeration system inside the main unit 1 and is pre-cooled to the set low temperature, completing the first heat extraction.

[0041] Secondary heat dissipation: Low-temperature coolant is pumped into the liquid-cooled radiator 3-3 inside the handheld collector 2. The liquid-cooled radiator 3-3 is in close contact with the hot end of the second refrigeration system. The low-temperature coolant efficiently removes the waste heat generated at the hot end of the second refrigeration system, forcibly reducing its hot end temperature to close to the coolant temperature.

[0042] Performance leap: According to the Peltier effect, the cold end temperature of the second cooling system is directly related to its hot end temperature. The hot end is efficiently cooled to a low temperature, which enables the cold end to generate an extremely low temperature very quickly and efficiently conduct the cooling energy to the condensing chamber 3-1 that is attached to it. The heat dissipation efficiency of the circulating liquid cooling is far superior to that of traditional air cooling, which solves the heat dissipation bottleneck of handheld devices and thus releases the maximum potential of the semiconductor cooling chip 3-2.

[0043] Further optimization of the scheme: The first refrigeration system includes a first liquid storage chamber 2A-4 for storing coolant, which is circulated and connected to the coolant circulation system. A spirally arranged evaporator 2A-3 is installed within the first liquid storage chamber 2A-4. The evaporator 2A-3, compressor 2A-1, condenser, and throttling device 2A-5 are connected in series to form a vapor compression refrigeration circuit to cool the coolant in the first liquid storage chamber 2A-4. Alternatively, the first refrigeration system can be a compressor 2A-1 refrigeration system. The compressor 2A-1, condenser 2A-2, evaporator 2A-3, and throttling device 2A-5 are connected in series via copper pipes to form a circuit for cooling the coolant in the first liquid storage chamber 2A-4. Its principle is similar to that of a refrigerator or air conditioner, and will not be elaborated further here.

[0044] In one embodiment of the present invention, the compressor 2A-1 is a small rotary compressor device, which is fixed to the bottom plate of the main unit 1 by a shock-absorbing pad to reduce vibration.

[0045] In one embodiment of the present invention, the condenser 2A-2 is a finned heat exchanger and is equipped with two axial fans for forced air cooling.

[0046] In one embodiment of the present invention, a spiral tube heat exchange module is selected, which is placed in the first liquid storage chamber 2A-4 for storing coolant and can directly exchange heat with the coolant.

[0047] In one embodiment of the present invention, the throttling device 2A-5 is a capillary tube with a length of approximately 1.2m and an outer diameter of φ2.5mm.

[0048] The design is further optimized. The coolant circulation system includes a second reservoir 2B-6, the outlet of which is connected to a liquid pump 2B-7. The outlet of the liquid pump 2B-7 is quickly connected to the flexible connection system 3 via a piping system. The coolant circulation system drives the coolant to circulate between the main unit 1 and the handheld collector 2. The liquid pump 2B-7 serves as the power source, providing circulation power for the coolant flowing out of the second reservoir 2B-6, allowing the coolant to flow along the piping system.

[0049] In one embodiment of the present invention, a temperature sensor is installed on the pipeline at the outlet of the second liquid storage chamber 2B-6 to monitor the temperature of the output coolant.

[0050] In one embodiment of the present invention, a PT1000 platinum resistance thermometer is selected as the temperature sensor.

[0051] In one embodiment of the present invention, the second liquid storage chamber 2B-6 is a stainless steel or polypropylene container with a capacity of about 2.5L, and has a liquid level observation window on the side.

[0052] In one embodiment of the present invention, the liquid pump 2B-7 is a DC24V brushless DC waterproof water pump with a maximum flow rate of 5L / min.

[0053] In one embodiment of the present invention, the piping system uses φ8mm polyurethane pipe, and the interface adopts a self-sealing quick-connect fitting or a pagoda-type connector with a tightening ring for sealing.

[0054] Further optimization of the design involves incorporating a U-shaped trough-like condensation chamber 3-1 within the insulation shell 3-4. This provides a larger condensation surface area and constrains and accelerates airflow. Several temperature sensors are mounted on the back of the condensation chamber 3-1. The handheld data collector 2 has a layered internal structure, centered on the U-shaped trough-like condensation chamber 3-1. From the inside out, the components are the condensation chamber 3-1, the semiconductor cooling chip 3-2, the liquid-cooled radiator 3-3, and the insulation shell 3-4. The insulation shell 3-4 is made of 6063 aluminum alloy and is CNC machined to include a U-shaped trough-like cavity serving as the condensation chamber 3-1. This provides a larger condensation surface area within a limited space, increasing the condensate collection efficiency to 3-10 times that of traditional equipment. It also constrains and accelerates airflow, promoting rapid condensate shedding and collection. Temperature sensors are used to monitor the temperature.

[0055] In one embodiment of the present invention, the continuous arc-shaped inner wall of the U-shaped groove of the condensation chamber 3-1 provides a larger surface area than a flat wall or a straight cylindrical wall within the same equipment cross-sectional area, thereby increasing the probability of water vapor colliding with and condensing on the cold wall surface.

[0056] In one embodiment of the invention, the U-shaped structure of the condenser cavity 3-1 naturally constrains and guides the airflow. According to fluid mechanics principles, the airflow experiences local acceleration and moderate turbulence as it flows through the upper arc-shaped region. This enhances the contact between the airflow and the entire condenser wall, disrupts the laminar boundary layer that is detrimental to heat transfer, and simultaneously, the accelerated airflow exerts stronger shear force on the liquid droplets adhering to the wall, causing them to be stripped and collected even when they are small in size.

[0057] In one embodiment of the present invention, the condensation chamber 3-1 has a depth of 35 mm, a width of 3 mm, and its surface is anodized.

[0058] Further optimizing the design, the second refrigeration system includes several sets of thermoelectric coolers 3-2. The cold end of the thermoelectric cooler 3-2 is attached to the back of the outer wall of the condensing cavity 3-1, and the hot end of the thermoelectric cooler 3-2 is attached to the surface of the liquid-cooled radiator 3-3. One or two sets of thermoelectric coolers 3-2, such as the TEC1-19906 type, are directly bonded to the back of the outer wall of the condensing cavity 3-1 using high thermal conductivity adhesive. The hot end of the thermoelectric cooler 3-2 contacts the surface of the liquid-cooled radiator 3-3 via high thermal conductivity adhesive. Simultaneously, bolt assemblies are used for tightening to ensure uniform pressure between the cold end of the thermoelectric cooler 3-2 and the condensing cavity 3-1, and between the hot end and the liquid-cooled radiator 3-3, minimizing contact thermal resistance.

[0059] In one embodiment of the present invention, the liquid-cooled radiator 3-3 is made of 6063 aluminum alloy and has an S-shaped channel inside as a coolant flow channel 3-6. The coolant flows through the coolant flow channel 3-6 to cool the condensation chamber 3-1.

[0060] In one embodiment of the present invention, the liquid-cooled radiators 3-3 on both sides of the condensation chamber 3-1 are connected in series by a series pipe 3-5, and the other two interfaces are connected to the flexible connection system 3 by an external pipe 3-7.

[0061] The sampling component is further optimized, including a condenser tube 4-1 that passes through a condensation chamber 3-1 and is press-fitted against the side wall of the condensation chamber 3-1. The bottom end of the condenser tube 4-1 is connected to a condensate collection tube 4-5 detachably connected to the bottom of the handheld sampler 2. The condenser tube 4-1 is the core component for sampling. Its middle section is inserted into the condensation chamber 3-1 through a U-shaped groove longitudinal opening 4-2. The press-fit ensures a large-area, tight physical contact between the outer wall of the condenser tube 4-1 and the inner wall of the condensation chamber 3-1, guaranteeing efficient heat conduction. When the user exhales into the condenser tube 4-1, the airflow is condensed and liquefied by the condensation chamber 3-1. A magnetic retainer ring 4-3 is installed at the bottom of the handheld sampler 2, which can be magnetically fixed or removed from the bottom fixing piece 4-4 of the handheld sampler 2. The magnetic retainer ring 4-3 is used to hold the collection tube 4-5, which collects the condensate flowing from the bottom of the condenser tube 4-1.

[0062] In one embodiment of the present invention, the condensation surface of the condenser tube 4-1 is designed with a thin wall of superhydrophobic and chemically inert material, and its outer wall is tightly fitted with the inner wall of the U-shaped condensation cavity 3-1 by means of interference fit, etc., which minimizes sample loss and contamination, and the non-specific adsorption rate of protein biomarkers is less than 3%, providing a more realistic and complete sample for downstream high-sensitivity analysis.

[0063] In one embodiment of the invention, the extremely low surface energy of the superhydrophobic surface causes water vapor to condense on it, forming discrete spherical droplets with a contact angle greater than 110°, resulting in droplet condensation rather than film condensation that spreads into a continuous insulating liquid film. The heat transfer efficiency of droplet condensation is more than an order of magnitude higher than that of film condensation, and the droplets are very easy to roll off under the influence of gravity or airflow.

[0064] In one embodiment of the present invention, the condenser tube 4-1 is preferably made of PTFE material. PTFE material has extremely stable chemical properties and low surface energy. Its non-specific interactions with biological macromolecules such as proteins, nucleic acids, and viruses, such as hydrophobic interactions and electrostatic adsorption, are very weak. This fundamentally avoids the adsorption and residue of target analytes such as protein adsorption <1% and virus adsorption <3% on the condenser surface, ensuring the originality of the chemical composition of the condensate.

[0065] In one embodiment of the present invention, rapid deep cooling instantly "fixes" unstable components in the sample, while the superhydrophobic surface avoids adsorption loss during the collection process. The two work together to maximize the protection of sample activity and integrity from both temporal and spatial dimensions.

[0066] Further optimizing the design, the flexible connection system 3 includes two parallel and independent coolant connecting pipes 5-1, with a stainless steel braided mesh 5-2 covering the outer wall of each coolant connecting pipe 5-1. The two coolant connecting pipes 5-1 are wrapped together by an insulation layer 5-3. The flexible connection system 3 adopts a three-layer composite structure. The two parallel coolant connecting pipes 5-1 are used for coolant inlet and outlet. Both ends of the coolant connecting pipes 5-1 are equipped with self-sealing quick connectors, which automatically open the sealing valve when inserted into the quick interface 1-3 of the host 1 and the collector interface 2-1 of the handheld collector 2, forming a sealed flow channel. The middle layer preferably consists of a stainless steel braided mesh 5-2 wrapped around the coolant connecting pipes 5-1 to enhance pressure resistance and bending resistance. The outer layer is a 2mm thick foamed polyethylene insulation layer 5-3 to minimize cold loss in the pipeline.

[0067] In one embodiment of the present invention, the flexible connection system 3 further includes a power line 5-4 for powering the handheld collector 2 and a shielded signal line 5-5 for transmitting control signals. The outer layer is entirely covered with an electromagnetic shielding layer and a wear-resistant rubber sheath, and both are wrapped in an insulation layer 5-3.

[0068] In one embodiment of the present invention, the power cord 5-4 is preferably an RV wire of 2.5 mm² or more.

[0069] In one embodiment of the present invention, the control system of this embodiment is responsible for the operation and status monitoring of the entire device.

[0070] This invention also discloses a method for collecting human exhaled condensate using a portable collection device based on a dual-circulation liquid cooling system, comprising the following steps: Connect and initialize the device, then start the first refrigeration system to pre-cool the coolant to the set temperature and enter the standby ready state; turn on the power of the host 1, the system performs a self-test, and detects the coolant capacity through the liquid level sensor. If the capacity is normal, it enters the pre-cooling stage and the first refrigeration system starts. After the first refrigeration system starts for 20 minutes, the coolant temperature drops from room temperature to 5°C. When the room temperature is 20°C, the pre-cooling liquid is 5L of pure water.

[0071] When the device is started, the second cooling system cools the working area of ​​the handheld data collector 2 to the set temperature. The user presses the condenser tube 4-1 into the U-shaped groove of the condenser chamber 3-1, and then presses the start button of the handheld data collector 2. The signal is transmitted to the host 1 through the flexible connection system 3. The main control system starts the second cooling system, with the initial power set to 50% to 100%. Then the liquid pump 2B-7 is accelerated to 100% flow rate and begins to monitor the temperature of the condenser chamber 3-1. During the first 1.5 minutes of the temperature control phase, full-power cooling is performed to quickly lower the temperature to -25℃.

[0072] The second cooling system switches its operating mode to maintain a stable temperature for the handheld sampler 2, then performs exhalation sampling. The collected sample condenses into liquid and collects. Switching to PID precise control, the temperature is stabilized at -30℃±2.5℃ by adjusting the power of the second cooling system (30%-100%) and the flow rate of the liquid pump 2B-7 (50%-100%). The user exhales deeply through the condenser tube 4-1, and the exhaled air condenses into droplets on the superhydrophobic inner wall of the condenser tube 4-1. The condensed droplets collect in the collection tube 4-5 under the influence of gravity and airflow.

[0073] After the preset sampling time or data collection amount is reached, the second cooling system is turned off or its power is reduced, but the first cooling system continues to run for a period of time; after the preset data collection time or data collection amount is reached, the second cooling system is turned off, but the liquid pump 2B-7 continues to run for 2 minutes to balance the system temperature.

[0074] The first refrigeration system was shut down, and the sample was taken for analysis.

[0075] Through the above technical solution, this device can achieve the following performance indicators: The cooling rate of the first refrigeration system is as follows: when the room temperature is 20℃ and the precooling liquid is 5L of pure water, the cooling time from 20℃ to 5℃ is less than or equal to 20 minutes, and the cooling time to 1℃ is less than or equal to 30 minutes.

[0076] At this time, when the second refrigeration system is turned on at 50% power, the cooling rate when the coolant is 1℃ and the precooling liquid is 5L of pure water is: from 1℃ to -20℃ in less than or equal to 3 minutes, and to 1℃ in less than or equal to 30 minutes. When the second refrigeration system is turned on at 100% power, the cooling rate is as follows (coolant temperature: 1℃, precooling liquid: 5L pure water): from 1℃ to -20℃ in less than or equal to 1.5 minutes, and to -30℃ in less than or equal to 2.5 minutes.

[0077] Temperature stability: Temperature fluctuation ≤ ±0.5℃ during operation (standby), and ≤ ±2.5℃ during data acquisition. Collection efficiency: 450-550 μL / min, deep breathing of an adult male, ambient temperature 20℃, relative humidity 40-60%. Sample adsorption rate: protein adsorption <1%, virus adsorption <3%.

[0078] In summary, the technical solution of this invention aims to: Solving the fundamental contradiction between cooling performance and equipment portability: Through an innovative "split-flexible connection" system architecture, the high-power, large-volume high-efficiency cooling unit (main unit 1) is physically separated from the lightweight sampling terminal (handheld collector 2). This overcomes the dilemma of traditional integrated devices that are either too bulky in pursuit of performance or sacrifice cooling depth in order to ensure portability. The device can simultaneously meet the portability requirements of clinical bedside and mobile diagnosis and treatment scenarios as well as the performance requirements of deep low temperature for scientific research.

[0079] Overcoming the inherent limitations of semiconductor cooling performance in handheld devices: By introducing a core working mechanism of "dual cooling system synergy and circulating liquid cooling heat dissipation", the first cooling system in the host 1 provides continuous and stable cooling to the circulating coolant, and then this low-temperature coolant is delivered to the data collector to efficiently dissipate heat from the hot end of the second cooling system. This aims to completely solve the core problems of weak cooling capacity and large temperature fluctuations caused by insufficient heat dissipation space and low air cooling efficiency of semiconductor cooling chips in traditional handheld devices, enabling the handheld data collector to quickly drop to and maintain deep cryogenic temperatures.

[0080] Improving the condensation capture efficiency of exhaled breath condensate samples and volatile components: By optimizing the combination structure of the superhydrophobic surface of the condensation chamber 3-1 with the condensation tube 4-1 of the U-shaped groove structure, the condensation specific surface area is maximized in a limited space. The airflow organization is optimized by using the U-shaped flow channel to enhance the airflow shear force. This overcomes the defects of insufficient airflow contact and serious liquid film retention in traditional straight cylindrical condensation chambers, thereby increasing the condensate collection efficiency to several times that of traditional equipment and significantly shortening the time required for a single sampling.

[0081] Ensuring sample authenticity and detection accuracy: By using a thin-walled condenser tube 4-1 with an inner surface made of a superhydrophobic material (such as Teflon or polyethylene), which serves as the condensation surface, the conversion and shedding of the condensate film into droplets can be effectively promoted. At the same time, it minimizes the non-specific adsorption of target analytes such as proteins and viruses in the sample by the condensation surface. Combined with the above-mentioned rapid deep low-temperature condensation process, sample degradation is inhibited, ensuring the accuracy and reliability of downstream analysis, thereby reducing sample loss and ensuring the accuracy and reliability of subsequent analysis results.

[0082] Example 2 Reference Figure 11 As shown, the difference between this embodiment and Embodiment 1 is only that the first refrigeration system includes at least two sets of cooling modules, and multiple sets of cooling modules are connected in parallel through valve group module 2B-5; the cooling modules are connected to the coolant circulation system to realize pre-cooling and cooling synergy between the cooling modules; the cooling module includes a semiconductor refrigeration chip group 2B-1, a hot end heat sink 2B-3 is attached to the hot end of the semiconductor refrigeration chip group 2B-1, a cold end liquid cooling plate 2B-2 is attached to the cold end of the semiconductor refrigeration chip group 2B-1, the outlet of the cold end liquid cooling plate 2B-2 is connected to the coolant circulation system, and the inlet of the cold end liquid cooling plate 2B-2 is connected to the valve group module 2B-5. The first refrigeration system can also be a semiconductor refrigeration system. The semiconductor refrigeration chip group 2B-1 uses two or more sets of semiconductor refrigeration chips, such as the TEC1-19906 model, arranged in parallel as the core component. The cold end liquid cooling plate 2B-2 is an aluminum alloy or copper plate with a size of about 200mm×40mm. It has flow channels processed inside. The cold end plane of the semiconductor refrigeration chip group 2B-1 is closely attached to one side of this liquid cooling plate. The hot end heat sink 2B-3 is a large finned heat sink connected to the hot end plane of the semiconductor refrigeration chip group 2B-1 and equipped with a cooling fan 2B-4 for forced heat dissipation.

[0083] In one embodiment of the present invention, high-performance thermally conductive silicone grease is applied between the semiconductor cooling chip group 2B-1, the cold end liquid cooling plate 2B-2, and the hot end heat sink 2B-3. Two or more cooling modules can be connected in series to form a whole to improve cooling efficiency. Preferably, multiple valve group modules 2B-5 are connected in parallel to realize the pre-cooling and cooling synergy between semiconductor cooling system groups.

[0084] Specific Example 1. Temperature Drop Rate Test of Precooling Liquid in the First Refrigeration System: In this invention, the first refrigeration system uses 5L of pure water as the coolant. At a room temperature of 20°C, the temperature drop rate of the coolant after precooling at 100% power is as follows: Figure 12As shown. Approximately 20 minutes after the cooling system started, the coolant temperature dropped from room temperature to 5°C, with a cooling rate of 0.75°C / min. After 28 minutes of cooling operation, the coolant temperature dropped to 0°C, with a cooling rate of 0.714°C / min. Simultaneously, the temperature of the condenser chamber 3-1 dropped to the same level.

[0085] Specific Example 2. Cooling Rate and Low-Temperature Maintenance Capability Test When Switching to Second Cooling System Power: Using 5L of pure water as coolant, and with a room temperature of 20℃, after the first cooling system was turned on at 100% power for pre-cooling, the coolant temperature dropped to 1.7℃. The second cooling system then switched between 50% / 100% power for rapid cooling and low-temperature maintenance. Figure 13 As shown. The temperature of the first refrigeration system was maintained between 1.7℃ and 5.5℃, with a temperature difference of 3.8℃ / 12.67min. After the second refrigeration system was turned on at 100% full power for 65s, the temperature of the collector's condenser chamber dropped from 0℃ to -20℃, with a cooling rate of -18.5℃ / min. The second refrigeration system was then turned on at 50% power to maintain the temperature at -20℃ for 2 minutes, with a temperature change of less than -2℃. After the second refrigeration system was turned on at 100% full power for 173s, the temperature of the collector's condenser chamber dropped from -22℃ to -33℃, with a cooling rate of -3.82℃ / min. Subsequently, after the second refrigeration system was turned off for 1.5min, the temperature of the condenser chamber rose to -22℃. The second refrigeration system was then turned on at 50% power to maintain the temperature at -22℃ for 103s. After the second refrigeration system was turned off, the first refrigeration system was turned on at full power for 2.4 minutes to cool the coolant, and the temperature of the circulating coolant dropped from 6.1℃ to 4℃.

[0086] Specific Example 3. Test of the second refrigeration system's 50% power cooling rate, low temperature maintenance, data acquisition, further cooling, and temperature maintenance capability: The first refrigeration system of this invention uses 5L of pure water as coolant. At a room temperature of 20℃, after the first refrigeration system is turned on at 100% power for pre-cooling, the coolant temperature drops to 1.2℃. The second refrigeration system's 50% power rapid cooling and low temperature maintenance capability is as follows: Figure 14 As shown in the diagram. The temperature of the first cooling system was maintained between 1.7℃ and 4.2℃, with a temperature difference of 2.5℃ / 10.8min. After the second cooling system was turned on at 50% full power for 166s, the temperature of the collector's condenser chamber dropped from 0.9℃ to -20℃, with a cooling rate of -7.59℃ / min. The second cooling system maintained the temperature at -20℃ at 50% power for 2.5 minutes, with a temperature change of less than -2℃. Subsequently, the condenser chamber temperature rose to -22℃, and the collection mode was activated for 115s. An adult male took 23 deep breaths, collecting 910ml of condensate. The condenser chamber temperature rose to -17℃, with a temperature change rate of 3.65℃ / min. After the collection was stopped, the second cooling system continued to operate at 50% power to cool down. After 2.8 minutes, the condenser chamber temperature dropped to -21℃, while the coolant temperature of the first cooling system remained at 4.2℃, with a temperature increase of only 0.2℃.

[0087] Specific Example 4. Second Refrigeration System 100% Power Cooling Rate - Low Temperature Maintenance - Data Acquisition - Re-cooling - Temperature Maintenance Capability Test: Using 5L of pure water as coolant, and with a room temperature of 20℃, after the first refrigeration system was turned on at 100% power for pre-cooling, the coolant temperature dropped to 1.2℃. The second refrigeration system's 100% power rapid cooling - low temperature maintenance capability is as follows: Figure 15 As shown. After 2.5 minutes of rapid cooling, the condenser temperature dropped from 1℃ to -30℃, with a cooling rate of -12.4℃ / min. The temperature of the first refrigeration system remained between 1.2℃ and 4.3℃, with a temperature difference change of 1.24℃ / min. Subsequently, within 16.5 minutes, the condenser entered a low-temperature maintenance phase, with the temperature dropping from -30℃ (coolant temperature 4.2℃) to -34℃ (coolant temperature 6.9℃) and then rising to -29℃ (coolant temperature 12.9℃, the upper limit of the coolant's maximum tolerance temperature). Subsequently, the cold storage capacity of the first refrigeration system and the coolant was insufficient to maintain the second refrigeration system at 100% full power output. After 8.6 minutes, the condenser temperature dropped from -29℃ to -25℃, and the coolant temperature of the first refrigeration system rapidly increased from 12.9℃ to 19.4℃.

[0088] Specific Example 5. Temperature Maintenance Capability Test of the Condensation Chamber in the Second Refrigeration System during the -33℃ Data Collection Process: Using 5L of pure water as the coolant, and with the room temperature at 20℃, the first refrigeration system of this invention was pre-cooled at 100% power. The condensation chamber was rapidly cooled to -33℃ (coolant temperature dropped to 6℃) during a deep breath EBC data collection process (110s) in an adult male. The low-temperature maintenance and data collection capabilities were as follows: Figure 16 As shown. The temperature of the first refrigeration system increased from 6℃ to 6.9℃; the temperature of the condenser chamber increased from -33℃ to -26℃. 920ul of EBC sample was collected after 32 breaths. After 100s of collection stopped, the condenser chamber was rewarmed to -30℃, and after 35s, it was rewarmed to -31℃ and maintained thereafter.

[0089] Specific Example 6. Coolant Low-Temperature Recooling Capacity Test: The first refrigeration system of the device of this invention uses 5L of pure water as coolant. At a room temperature of 20℃, after completing EBC data acquisition and shutting down the second refrigeration system, the coolant cooling and recooling rate is as follows: Figure 17 The first refrigeration system, operating at 100% power, reduced the coolant temperature from 7.6℃ to 3.5℃ in the 5th minute of cooling, a temperature change of 0.82℃ / min; and in the 10th minute, the coolant temperature dropped from 7.6℃ to 1.2℃, a temperature change of 0.54℃ / min. This means that once the coolant temperature does not exceed 7.6℃ after one round of EBC data acquisition, the system can perform a brief pre-cooling cycle for rapid cooling.

[0090] In the description of this invention, it should be understood that the terms "longitudinal", "lateral", "up", "down", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this invention, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this invention.

[0091] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A portable collection device for human exhaled breath condensate from a dual-circulation liquid cooling system, characterized in that: It includes a host (1), a handheld collector (2) and a flexible connection system (3), wherein the flexible connection system (3) is connected between the host (1) and the handheld collector (2); The host (1) includes a first refrigeration system and a coolant circulation system. The first refrigeration system is used to perform primary cooling of the coolant, and the coolant circulation system is used to drive the coolant to circulate between the first refrigeration system and the handheld collector (2). The handheld collector (2) includes an insulated shell (3-4), inside which a second refrigeration system and a liquid-cooled radiator (3-3) are provided. The second refrigeration system and the liquid-cooled radiator (3-3) are connected by heat conduction. The second refrigeration system is provided with a sampling component for sampling. The liquid-cooled radiator (3-3) is connected to the first refrigeration system through the flexible connection system (3).

2. The portable collection device for exhaled breath condensate from a dual-cycle liquid cooling system according to claim 1, characterized in that: The first refrigeration system includes a first liquid storage chamber (2A-4) for storing coolant, which is circulated in connection with the coolant circulation system. A spirally arranged evaporator (2A-3) is installed in the first liquid storage chamber (2A-4). The evaporator (2A-3) is connected in series with the compressor (2A-1), the condenser and the throttling device (2A-5) to form a vapor compression refrigeration circuit to cool the coolant in the first liquid storage chamber (2A-4).

3. The portable collection device for exhaled breath condensate from a dual-cycle liquid cooling system according to claim 1, characterized in that: The first refrigeration system includes at least two sets of cooling modules, and multiple sets of cooling modules are connected in parallel through valve group modules (2B-5); the cooling modules are connected to the coolant circulation system to realize pre-cooling and cooling synergy among the cooling modules.

4. The portable collection device for exhaled breath condensate from a dual-cycle liquid cooling system according to claim 3, characterized in that: The cooling module includes a semiconductor refrigeration chip assembly (2B-1), a hot-end heat sink (2B-3) is attached to the hot end of the semiconductor refrigeration chip assembly (2B-1), a cold-end liquid cooling plate (2B-2) is attached to the cold end of the semiconductor refrigeration chip assembly (2B-1), the outlet of the cold-end liquid cooling plate (2B-2) is connected to the coolant circulation system, and the inlet of the cold-end liquid cooling plate (2B-2) is connected to the valve assembly module (2B-5).

5. The portable collection device for exhaled breath condensate from a dual-cycle liquid cooling system according to claim 1, characterized in that: The coolant circulation system includes a second reservoir (2B-6), the outlet of which is connected to a liquid pump (2B-7), and the outlet of the liquid pump (2B-7) is quickly connected to the flexible connection system (3) through a pipeline system.

6. The portable collection device for exhaled breath condensate from a dual-cycle liquid cooling system according to claim 1, characterized in that: The heat-insulating outer shell (3-4) is provided with a U-shaped groove cavity structure condensation chamber (3-1), which has a larger condensation surface area and constrains and accelerates the airflow; several temperature sensors are installed on the back of the condensation chamber (3-1).

7. The portable collection device for exhaled gas condensate from a dual-cycle liquid cooling system according to claim 6, characterized in that: The second refrigeration system includes several sets of semiconductor refrigeration chips (3-2), the cold end of the semiconductor refrigeration chip (3-2) is attached to the back of the outer wall of the condensation cavity (3-1), and the hot end plane of the semiconductor refrigeration chip (3-2) is attached to the surface of the liquid cooling radiator (3-3).

8. The portable collection device for exhaled breath condensate from a dual-cycle liquid cooling system according to claim 6, characterized in that: The sampling assembly also includes a condenser tube (4-1), which passes through the condensation chamber (3-1) and is press-fitted to the side wall of the condensation chamber (3-1); the bottom end of the condenser tube (4-1) is connected to a condensate collection tube (4-5) that is detachably connected to the bottom end of the handheld collector (2).

9. The portable collection device for exhaled breath condensate from a dual-cycle liquid cooling system according to claim 1, characterized in that: The flexible connection system (3) includes two parallel and independent coolant connecting pipes (5-1), the outer wall of which is fitted with a stainless steel braided mesh (5-2); the two coolant connecting pipes (5-1) are wrapped together by an insulation layer (5-3).

10. A method for collecting human exhaled breath condensate, based on the portable human exhaled breath condensate collection device of the dual-circulation liquid cooling system according to any one of claims 1-9, characterized in that, Includes the following steps: Connect and initialize the device, then start the first refrigeration system to pre-cool the coolant to the set temperature, and enter the standby ready state; Start the equipment, and the second cooling system cools the working area of ​​the handheld data collector (2) to the set temperature; The second cooling system switches to a different working mode to maintain the temperature of the handheld sampler (2) and then performs breath sampling. The collected samples condense into liquid and collect. After the preset sampling time or data collection time is reached, the second cooling system shuts down or reduces its power, but the first cooling system continues to run for a period of time. The first refrigeration system was shut down, and the sample was taken for analysis.

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