Culture and domestication method of salt-tolerant heterotrophic nitrification and denitrification microbial agent

By cultivating and domesticating the salt-tolerant heterotrophic nitrifying and denitrifying microbial agent Vreelandella titanicaestrain SOB56, the problem of low microbial denitrification efficiency under high salinity conditions was solved, achieving efficient simultaneous nitrification and denitrification, and simultaneous treatment of marine aquaculture wastewater, thus solving the problem of efficient denitrification of marine aquaculture wastewater.

CN121801797APending Publication Date: 2026-04-07DONGGUAN UNIV OF TECH
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Patent Information

Application Number
CN202610020349.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-01-08
Publication Date
2026-04-07

AI Technical Summary

Technical Problem

Microbial denitrification is inefficient in high-salinity environments. Traditional denitrification processes are time-consuming and energy-intensive. The separate steps of nitrification and denitrification extend the treatment time and affect the treatment effect of marine aquaculture wastewater.

Method used

The salt-tolerant heterotrophic nitrifying and denitrifying microbial agent, *Vreelandella titanicaestrain* SOB56, was cultured and domesticated for use in the treatment of marine aquaculture wastewater, achieving simultaneous nitrification and denitrification and reducing ammonia nitrogen and nitrate nitrogen content.

Benefits of technology

Under high salinity conditions, the strain effectively removes nitrogenous substances from marine aquaculture wastewater, with removal rates as high as 99.6% and 94.22%, without the formation of nitrate and nitrite nitrogen. It is adaptable to high-salinity wastewater of different salinity and has broad application prospects.

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Abstract

The invention discloses a culture and domestication method of a salt-tolerant heterotrophic nitrification and denitrification microbial agent, and belongs to the technical field of environmental microorganisms. Comprising the following steps: inoculating a heterotrophic nitrification culture medium with a halomonas seed solution, and culturing until a strain reaches a stable growth period to finish domestication. The Halomonas sp. SOB56 strain provided by the invention can be used for effectively removing nitrogen-containing substances in high-salinity wastewater such as mariculture wastewater and the like, and can be adapted to high-salinity wastewater with different salinity through domestication. The removal rates of the strain on ammonia nitrogen and nitrate nitrogen are 99.6% and 94.22% respectively within 36 hours when the strain enters a logarithmic phase under the condition that the temperature is 30 DEG C and the salinity is 5%. The biological nitrogen removal method belongs to the technical field of novel biological nitrogen removal, nitrate nitrogen and nitrite nitrogen are not generated in the ammonia nitrogen removal process, and the biological nitrogen removal method has a good application prospect in actual application of high-salinity wastewater such as mariculture wastewater.
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