Orange fluorescent silicon nanodot, preparation method thereof and application of orange fluorescent silicon nanodot in imaging of mitochondria in living cells
By preparing orange fluorescent silicon nanodots, the problem of poor imaging effect of fluorescent materials in live cell mitochondria in existing technologies has been solved, achieving efficient and stable mitochondrial imaging effect, and has broad prospects for bioimaging and sensing applications.
CN121825537APending Publication Date: 2026-04-10SUZHOU INST OF MEDICAL ENG CHINESE ACAD OF SCI ZHENGZHOU INST OF ENG TECH +1
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Patent Information
- Application Number
- CN202511777045.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-11-28
- Publication Date
- 2026-04-10
AI Technical Summary
Technical Problem
The lack of efficient, low-toxicity, and photostable fluorescent materials in the current technology for live-cell mitochondrial imaging leads to poor imaging results.
Method used
Orange fluorescent silicon nanodots were prepared using tetramethylrhodamine methyl ester and N-(2-aminoethyl)-3-aminopropyltrimethoxysilane as raw materials via hydrothermal reaction and column chromatography purification for imaging mitochondria in live cells.
Benefits of technology
The prepared orange fluorescent silicon nanodots (O-SiNDs) have high quantum yield, good photostability, and can specifically target and label mitochondria of living cells. They also have excellent tissue penetration ability and resistance to background interference, making them suitable for bioimaging and sensing.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention discloses an orange fluorescent silicon nanodot, a preparation method thereof and an application of the orange fluorescent silicon nanodot in mitochondrial imaging in living cells, the method comprises the following steps: S1, dissolving tetramethyl rhodamine methyl ester in ultrapure water, then adding N-(2-aminoethyl)-3-aminopropyltrimethoxysilane, uniformly stirring, and carrying out hydrothermal reaction on the obtained mixture; and S2, cooling after the reaction is finished, purifying the product by using a chromatographic column, eluting by using an eluent, collecting the eluent, and drying to obtain the orange fluorescent silicon nanodot. The silicon nanodot provided by the invention has the advantages of good light stability, high quantum yield and the like, and can be successfully applied to positioning imaging of mitochondria in living cells; compared with common blue-green fluorescence, orange fluorescence has better tissue penetrating ability and background interference resistance; the O-SiNDs prepared by the invention also has the property similar to that of lipophilic cations, and can realize high-specificity targeted marking on mitochondria of living cells.
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