Traditional Chinese medicine extract feed additive for treating pullorum disease and preparation method thereof

Abstract

The invention relates to the technical field of feed additives, in particular to a traditional Chinese medicine extract feed additive for treating pullorum disease and a preparation method thereof. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 10-15 parts of radix scutellariae, 6-9 parts of rhizoma coptidis, 4-6 parts of fried radix sanguisorbae, 3-5 parts of herba portulacae, 2-3 parts of horseradish tree leaves, 1-2 parts of fructus aurantii immaturus and 1-2 parts of pouzolzia zeylanica. The invention also provides a preparation method of the feed additive, and the prepared feed additive can effectively treat pullorum disease.

Description

Technical Field

[0001] This invention relates to the field of feed additive technology, specifically to a feed additive made from a traditional Chinese medicine extract for treating chicken diarrhea and its preparation method. Background Technology

[0002] Pullorum disease (African pullorum disease) is a common, highly contagious, and frequently occurring infectious disease caused by Salmonella pullorum. It is extremely harmful to chicks, leading to high mortality rates and significant economic losses to the poultry industry. The disease can be transmitted vertically and horizontally, but it is most prevalent in chicks. Affected chicks exhibit symptoms such as grayish-white droppings, chills, huddling together, loss of appetite, difficulty breathing, and even death. The disease can occur year-round, but it is most common in chicks under 3 weeks old, with mortality rates reaching 10%-50%. As chicks age, their resistance increases, and adult chickens may develop chronic or latent infections or become carriers, posing a dangerous source of infection. Currently, the traditional treatment method involves adding antibiotics to the feed. However, long-term antibiotic use easily leads to increased bacterial resistance, drug residues, decreased poultry product quality, and environmental damage. It not only disrupts the normal flora of the chicken's digestive tract, causing drug-induced diseases and drug residues, but also leads to the emergence of drug-resistant strains, resulting in poor prevention and control effects, increasing breeding costs, and causing excessive drug residues. Summary of the Invention

[0003] To address the shortcomings of existing technologies, this invention proposes a feed additive made from a traditional Chinese medicine extract for treating chicken diarrhea and its preparation method.

[0004] This invention is achieved through the following technical solution: A feed additive made from a traditional Chinese medicine extract for treating chicken diarrhea, the raw materials for which are prepared include the following components in parts by weight: 10-15 parts of Scutellaria baicalensis, 6-9 parts of Coptis chinensis, 4-6 parts of stir-fried Sanguisorba officinalis, 3-5 parts of Portulaca oleracea, 2-3 parts of Moringa oleifera leaves, 1-2 parts of Citrus aurantium, and 1-2 parts of Pueraria lobata.

[0005] Furthermore, the preparation method of the feed additive made from the traditional Chinese medicine extract for treating fowl dysentery includes the following steps: S1. Wash and dry the Scutellaria baicalensis, Coptis chinensis, stir-fried Sanguisorba officinalis, Portulaca oleracea, Moringa oleifera leaves, Citrus aurantium, and Pueraria lobata, respectively. Grind them into powder and pass them through an 80-100 mesh sieve. Mix them evenly to obtain a mixed Chinese herbal powder. Add the mixed Chinese herbal powder to boiling water and keep it warm for 1 hour. Reflux for 2 hours and filter. Add the residue to boiling water again and reflux for 1 hour. Filter again and combine the two filtrates to obtain the Chinese herbal extract. S2. Take the herbal extract obtained in step S1, sterilize at 115℃ for 30 min, cool, inoculate with activated Lactobacillus paracasei and Bifidobacterium animalis, ferment at 37℃ for 24-28 h, centrifuge at 10000 rpm for 8-10 min, filter the supernatant through a 0.22 μm filter membrane, concentrate under reduced pressure, freeze dry, and obtain the herbal fermentation agent. S3. Wash and dry the Sargassum fusiforme, pulverize it through a 40-60 mesh sieve to obtain Sargassum fusiforme powder. Add the Sargassum fusiforme powder to 95% ethanol, reflux at 95-100℃ for 3 h to defatt it, filter and collect the residue, dry it to obtain defatted Sargassum fusiforme powder, add the defatted Sargassum fusiforme powder to deionized water, sonicate at 200 W for 20-25 min, extract at 100℃ for 2-3 h, and centrifuge at 8000 r / min for 10-15 min. S4. After centrifugation in step S3, take the supernatant, concentrate it under vacuum to 20%-25% of the original volume, add 4 times the volume of anhydrous ethanol, let it stand at 4℃ for 12 h, centrifuge at 8000 r / min for 10 min, take the precipitate, vacuum dry it, reconstitute it with water, freeze dry it, mix the freeze-dried product with the traditional Chinese medicine fermentation agent obtained in step S2 at a mass ratio of 1:5 to obtain a feed additive of traditional Chinese medicine extract for treating chicken white diarrhea.

[0006] Further, in step S1, the ratio of the mixed Chinese herbal powder to boiling water is 1 g: 10 mL.

[0007] Further, in step S2, the Lactobacillus paracasei and Bifidobacterium animalis are inoculated at volume fractions of 2% and 3%, respectively.

[0008] Furthermore, in step S2, the viable count of both *Lactobacillus paracasei* and *Bifidobacterium animalis* is 5 × 10⁻⁶. 8 CFU / mL.

[0009] Furthermore, in step S3, the ratio of Sargassum powder to 95% ethanol is 1 g: 4-5 mL.

[0010] Furthermore, in step S3, the ratio of defatted Sargassum powder to deionized water is 1 g: 50 mL.

[0011] Compared with the prior art, the present invention has the following beneficial effects: This invention provides a feed additive made from traditional Chinese medicine extracts for treating pullorum disease in chickens. Through the combination of compound traditional Chinese medicines, it synergistically clears heat and dries dampness, fights bacteria and stops diarrhea. Probiotic fermentation enhances bioavailability, and the addition of polysaccharides extracted from Sargassum fusiforme enhances immunity. These synergistic effects inhibit Salmonella pullorum, repair the intestinal barrier, regulate the microecology, and enhance the body's resistance, thus effectively preventing and treating pullorum disease in chickens while avoiding antibiotic resistance and residue problems. This invention uses a scientific combination of carefully selected traditional Chinese medicines, which are antibacterial and anti-inflammatory, inhibit Salmonella pullorum, detoxify and stop diarrhea, regulate the intestinal environment, kill pathogens, regulate overall function, and repair the intestinal barrier. This invention involves inoculating the traditional Chinese medicine extract with Lactobacillus paracasei and Bifidobacterium animalis for fermentation. This can convert large molecular active substances (such as flavonoids and polysaccharides) in the traditional Chinese medicine into smaller molecular aglycones or secondary metabolites that are more easily absorbed, significantly improving bioavailability. The fermentation process may modify the chemical structure of some traditional Chinese medicine components, enhancing their antibacterial activity while reducing potential toxicity or bitterness. The organic acids (lactic acid, acetic acid, etc.) produced by probiotics during their own metabolism synergistically enhance the overall antibacterial effect with the components of traditional Chinese medicine. This invention extracts polysaccharides from Sargassum fusiforme, which possess immunomodulatory activity, enhance the body's immunity, strengthen antibacterial effects, regulate the intestinal microecological balance of chickens, enhance intestinal barrier function, reduce pathogenic bacteria colonization, supplement nutrition, and improve the chicken's resistance. Furthermore, it synergistically enhances the therapeutic effect when combined with traditional Chinese medicine fermentation agents. Attached Figure Description

[0012] Figure 1 The feed additives described in Examples 1-3 and Comparative Examples 1-3 of this invention have a preventive effect on chicken pullorum disease. Figure 2 This demonstrates the antibacterial effect of the feed additives described in Examples 1-3 and Comparative Examples 1-3 of the present invention. Figure 3 The therapeutic effects of the feed additives described in Examples 1-3 and Comparative Examples 1-3 of this invention on chicken pullorum disease are shown. Detailed Implementation

[0013] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to specific embodiments. However, this invention is not limited to the following embodiments. It should be noted that, unless otherwise specified, all chemical reagents involved in this invention are purchased through commercial channels.

[0014] Example 1: A feed additive made from a traditional Chinese medicine extract for treating chicken diarrhea, the raw materials for which are prepared include the following components in parts by weight: 15 parts Scutellaria baicalensis, 9 parts Coptis chinensis, 6 parts stir-fried Sanguisorba officinalis, 5 parts Portulaca oleracea, 3 parts Moringa oleifera leaves, 2 parts Citrus aurantium, and 2 parts Pueraria lobata.

[0015] A method for preparing a feed additive based on a traditional Chinese medicine extract for treating fowl dysentery includes the following steps: S1. According to the weight parts, Scutellaria baicalensis, Coptis chinensis, stir-fried Sanguisorba officinalis, Portulaca oleracea, Moringa oleifera leaves, Citrus aurantium, and Pueraria lobata were washed, dried, pulverized and passed through a 100-mesh sieve, and mixed evenly to obtain mixed Chinese herbal powder. 100 g of the mixed Chinese herbal powder was added to 1 L of boiling water, kept warm and soaked for 1 h, refluxed for 2 h, filtered, and the residue was added to 1 L of boiling water again and refluxed for 1 h, filtered, and the two filtrates were combined to obtain Chinese herbal extract. S2. Take the herbal extract obtained in step S1, sterilize at 115℃ for 30 min, cool, and inoculate with 5×10⁻⁶ viable bacteria. 8 Lactobacillus paracasei and Bifidobacterium animalis were inoculated at CFU / mL, with Lactobacillus paracasei and Bifidobacterium animalis at volume fractions of 2% and 3%, respectively. Fermentation was carried out at 37℃ for 28 h, centrifuged at 10000 rpm for 10 min, and the supernatant was filtered through a 0.22 μm filter membrane, concentrated under reduced pressure, and freeze-dried to obtain the traditional Chinese medicine fermentation agent. S3. Wash and dry the Sargassum fusiforme, pulverize it through a 60-mesh sieve to obtain Sargassum fusiforme powder. Add 50 g of Sargassum fusiforme powder to 250 mL of 95% ethanol, reflux at 100℃ for 3 h to defatt it, filter and collect the residue, dry it to obtain defatted Sargassum fusiforme powder. Take the defatted Sargassum fusiforme powder and add it to deionized water at a material-to-liquid ratio of 1 g: 50 mL, sonicate at 200 W for 25 min, extract at 100℃ for 3 h, and centrifuge at 8000 r / min for 15 min. S4. After centrifugation in step S3, take the supernatant, concentrate it under vacuum to 25% of the original volume, add 4 times the volume of anhydrous ethanol, let it stand at 4℃ for 12 h, centrifuge at 8000 r / min for 10 min, take the precipitate, vacuum dry it, reconstitute it with water, freeze dry it, mix the freeze-dried product with the traditional Chinese medicine fermentation agent obtained in step S2 at a mass ratio of 1:5 to obtain a feed additive of traditional Chinese medicine extract for treating chicken white diarrhea.

[0016] Example 2: A feed additive made from a traditional Chinese medicine extract for treating chicken diarrhea, the raw materials for which are prepared include the following components in parts by weight: 10 parts Scutellaria baicalensis, 6 parts Coptis chinensis, 4 parts stir-fried Sanguisorba officinalis, 3 parts Portulaca oleracea, 2 parts Moringa oleifera leaves, 1 part Citrus aurantium, and 1 part Pueraria lobata.

[0017] A method for preparing a feed additive based on a traditional Chinese medicine extract for treating fowl dysentery includes the following steps: S1. According to the weight parts, Scutellaria baicalensis, Coptis chinensis, stir-fried Sanguisorba officinalis, Portulaca oleracea, Moringa leaves, Citrus aurantium, and Pueraria lobata were washed, dried, pulverized and passed through an 80-mesh sieve, and mixed evenly to obtain mixed Chinese herbal powder. 100 g of the mixed Chinese herbal powder was added to 1 L of boiling water, kept warm and soaked for 1 h, refluxed for 2 h, filtered, and the residue was added to 1 L of boiling water again and refluxed for 1 h, filtered, and the two filtrates were combined to obtain Chinese herbal extract. S2. Take the herbal extract obtained in step S1, sterilize at 115℃ for 30 min, cool, and inoculate with 5×10⁻⁶ viable bacteria. 8 Lactobacillus paracasei and Bifidobacterium animalis were inoculated at CFU / mL, with Lactobacillus paracasei and Bifidobacterium animalis at volume fractions of 2% and 3%, respectively. Fermentation was carried out at 37℃ for 24 h, centrifuged at 10000 rpm for 8 min, and the supernatant was filtered through a 0.22 μm filter membrane, concentrated under reduced pressure, and freeze-dried to obtain the traditional Chinese medicine fermentation agent. S3. Wash and dry the Sargassum fusiforme, pulverize it through a 40-mesh sieve to obtain Sargassum fusiforme powder. Add 50 g of Sargassum fusiforme powder to 200 mL of 95% ethanol, reflux at 95℃ for 3 h to defatt it, filter and collect the residue, dry it to obtain defatted Sargassum fusiforme powder. Take the defatted Sargassum fusiforme powder and add it to deionized water at a material-to-liquid ratio of 1 g: 50 mL, sonicate at 200 W for 20 min, extract at 100℃ for 2 h, and centrifuge at 8000 r / min for 10 min. S4. After centrifugation in step S3, take the supernatant, concentrate it under vacuum to 20% of the original volume, add 4 times the volume of anhydrous ethanol, let it stand at 4℃ for 12 h, centrifuge at 8000 r / min for 10 min, take the precipitate, vacuum dry it, reconstitute it with water, freeze dry it, mix the freeze-dried product with the traditional Chinese medicine fermentation agent obtained in step S2 at a mass ratio of 1:5 to obtain a feed additive of traditional Chinese medicine extract for treating chicken white diarrhea.

[0018] Example 3: A feed additive made from a traditional Chinese medicine extract for treating chicken diarrhea, the raw materials of which include the following components in parts by weight: 12 parts Scutellaria baicalensis, 8 parts Coptis chinensis, 5 parts stir-fried Sanguisorba officinalis, 4 parts Portulaca oleracea, 2.5 parts Moringa oleifera leaves, 1.5 parts Citrus aurantium, and 1.2 parts Pueraria lobata.

[0019] A method for preparing a feed additive based on a traditional Chinese medicine extract for treating fowl dysentery includes the following steps: S1. According to the weight parts, Scutellaria baicalensis, Coptis chinensis, stir-fried Sanguisorba officinalis, Portulaca oleracea, Moringa oleifera leaves, Citrus aurantium, and Pueraria lobata were washed, dried, pulverized and passed through a 90-mesh sieve, and mixed evenly to obtain mixed Chinese herbal powder. 100 g of the mixed Chinese herbal powder was added to 1 L of boiling water, kept warm and soaked for 1 h, refluxed for 2 h, filtered, and the residue was added to 1 L of boiling water again and refluxed for 1 h, filtered, and the two filtrates were combined to obtain Chinese herbal extract. S2. Take the herbal extract obtained in step S1, sterilize at 115℃ for 30 min, cool, and inoculate with 5×10⁻⁶ viable bacteria. 8 Lactobacillus paracasei and Bifidobacterium animalis were inoculated at CFU / mL, with Lactobacillus paracasei and Bifidobacterium animalis at volume fractions of 2% and 3%, respectively. Fermentation was carried out at 37℃ for 26 h, centrifuged at 10000 rpm for 9 min, and the supernatant was filtered through a 0.22 μm filter membrane, concentrated under reduced pressure, and freeze-dried to obtain the traditional Chinese medicine fermentation agent. S3. Wash and dry the Sargassum fusiforme, pulverize it through a 50-mesh sieve to obtain Sargassum fusiforme powder. Add 50 g of Sargassum fusiforme powder to 240 mL of 95% ethanol, reflux at 98℃ for 3 h to defatt it, filter and collect the residue, dry it to obtain defatted Sargassum fusiforme powder. Take the defatted Sargassum fusiforme powder and add it to deionized water at a material-to-liquid ratio of 1 g: 50 mL, sonicate at 200 W for 22 min, extract at 100℃ for 2.5 h, and centrifuge at 8000 r / min for 12 min. S4. After centrifugation in step S3, take the supernatant, concentrate it under vacuum to 24% of the original volume, add 4 times the volume of anhydrous ethanol, let it stand at 4℃ for 12 h, centrifuge at 8000 r / min for 10 min, take the precipitate, vacuum dry it, reconstitute it with water, freeze dry it, mix the freeze-dried product with the traditional Chinese medicine fermentation agent obtained in step S2 at a mass ratio of 1:5 to obtain a feed additive of traditional Chinese medicine extract for treating chicken diarrhea.

[0020] The only difference between Comparative Example 1 and Example 1 is that the Chinese herbal extract obtained in step S1 is concentrated under reduced pressure and freeze-dried, and the resulting product is used to replace the Chinese herbal fermentation agent.

[0021] The only difference between Comparative Example 2 and Example 1 is that a traditional Chinese medicine fermentation agent was used instead of the feed additive containing the traditional Chinese medicine extract used to treat chicken diarrhea.

[0022] The only difference between Comparative Example 3 and Example 1 is that only Astragalus membranaceus and Coptis chinensis were used to prepare the Chinese medicine powder.

[0023] Example 1: Salmonella pullorum was inoculated onto nutrient agar plates and incubated at 37°C for 18 h. Before challenge, the bacterial growth was scraped off with a cotton swab and mixed thoroughly in sterile physiological saline to prepare a bacterial concentration of 9 × 10⁻⁶. 8 The bacterial suspension was prepared at CFU / mL. One hundred and twenty healthy one-day-old chickens (vaccinated against Marek's virus at one day old) were randomly divided into eight groups of 15 chickens each: six experimental groups (Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2, and Comparative Example 3), a positive control group (infected but not treated), and a blank control group (not infected and not treated). The experiment was conducted in the same chicken house, with chickens raised on wire mesh and allowed free access to feed and water at set times. The feed was chick feed formulated according to national nutritional standards. Ventilation was maintained, and temperature and humidity were carefully controlled. Feces were removed once daily. The experimental groups were fed feed supplemented with the feed additives obtained in Examples 1-3 and Comparative Examples 1-3, respectively, while the positive control and blank control groups were fed feed without the feed additives of this invention. The feed additive dosage was 1 g / kg. At six days of age, each chicken in the experimental and positive control groups was orally administered 9 × 10⁹ g of the feed additive. 8 1 mL of cfu / mL Salmonella pullorum was used to infect chickens. Mortality rates were calculated for each group 7 days after infection. Results are as follows: Figure 1As shown.

[0024] Figure 1 The results showed that the mortality rate in the blank control group was 0, while the mortality rate in the positive control group increased to 46.67% after infection with Salmonella pullorum. The mortality rates of Examples 1-3 and Comparative Examples 1-3 were significantly lower than those in the positive control group. Among them, the mortality rate of Comparative Examples 1-3 was higher than that of Examples 1-3. Comparative Example 1 used a water extract of traditional Chinese medicine to replace the fermentation agent of traditional Chinese medicine, Comparative Example 2 used a fermentation agent of traditional Chinese medicine to replace the feed additive of traditional Chinese medicine extract for treating pullorum disease, and Comparative Example 3 used only Astragalus membranaceus and Coptis chinensis to prepare traditional Chinese medicine powder. All of the above operations reduced the efficacy of the feed additive. The feed additive of traditional Chinese medicine extract for treating pullorum disease prepared by this invention has a synergistic effect among its components, effectively reducing the mortality rate.

[0025] Experimental Example 2: Take 1 g each of the feed additives prepared in Examples 1-3 and Comparative Examples 1-3, add them to 10 mL of deionized water and disperse evenly to obtain a feed additive solution. Take a contaminated nutrient agar medium, divide it into 6 groups, and use cotton swabs to apply a 1×10⁻⁶ g solution to each group. 5 CFU / L of Salmonella pullorum suspension was evenly spread on the surface of the culture medium. Then, an Oxford cup (a small circular tube with an inner diameter of 6 mm, an outer diameter of 8 mm, and a height of 10 mm) was placed vertically directly on the surface of the medium, and gentle pressure was applied to ensure seamless contact between the Oxford cup and the culture medium. 240 μL of feed additive solution was added to the Oxford cup, and the mixture was incubated at 37°C for 18 h. The size of the inhibition zone was measured, and the results are as follows: Figure 2 As shown.

[0026] Figure 2 The results showed that the diameter of the inhibition zone in Examples 1-3 was significantly larger than that in Comparative Examples 1-3. Comparative Example 1 used a water extract of traditional Chinese medicine to replace the fermentation agent of traditional Chinese medicine, Comparative Example 2 used a fermentation agent of traditional Chinese medicine to replace the feed additive of traditional Chinese medicine extract for treating fowl pullorum disease, and Comparative Example 3 used only Astragalus membranaceus and Coptis chinensis to prepare traditional Chinese medicine powder. All of the above operations reduced the antibacterial effect of the feed additive. The feed additive of traditional Chinese medicine extract for treating fowl pullorum disease prepared by this invention has a synergistic effect among its components and effectively inhibits Salmonella pullorum.

[0027] Experiment Example 3: Healthy 1-day-old chickens were raised to 7 days old. Each chicken was gavaged with 0.5 mL of Salmonella pullorum solution. When a group infection occurred (chicks began to leave the flock, and white diarrhea appeared in their feces), typical infected chicks were selected as the standard disease model. Chicks showing infection symptoms were randomly divided into 6 groups (Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2, and Comparative Example 3), with 15 chicks in each group. Each group was fed with feed supplemented with the feed additives obtained in Examples 1-3 and Comparative Examples 1-3, respectively. The feed additive dosage was 1 g / kg, for 7 consecutive days. After the experiment, the treatment effect was categorized as significant, effective, and ineffective based on the symptoms of the affected chickens. Significant effect meant the disappearance of adverse symptoms, a significant improvement in mental state, a gradual recovery in feed intake, and a change in feces from loose to normal consistency. Effective meant some improvement in adverse symptoms, and some recovery in appetite and mental state. Ineffective meant no improvement in symptoms, or even worsening of symptoms. The overall effective rate was calculated. Overall effectiveness (%) = [(Number of effective birds + Number of effective birds) / Total number of birds] × 100%. Results are as follows: Figure 3 As shown.

[0028] Figure 3 The results showed that the feed additives in Examples 1-3 and Comparative Examples 1-3 were all effective in treating chicken pullorum disease. The effect of Examples 1-3 was better than that of Comparative Examples 1-3. Comparative Example 1 used a water extract of traditional Chinese medicine to replace the fermentation agent of traditional Chinese medicine, Comparative Example 2 used the fermentation agent of traditional Chinese medicine to replace the feed additive of traditional Chinese medicine extract for treating chicken pullorum disease, and Comparative Example 3 only used Astragalus membranaceus and Coptis chinensis to prepare traditional Chinese medicine powder. All of the above operations reduced the therapeutic effect of the feed additives. The feed additive of traditional Chinese medicine extract for treating chicken pullorum disease prepared by this invention has synergistic effects among its components, which can effectively inhibit bacteria and improve the treatment efficacy.

[0029] Those skilled in the art should understand that the discussion of any of the above embodiments is merely exemplary and is not intended to imply that the scope of the invention is limited to these examples; within the framework of the invention, the technical features of the above embodiments or different embodiments can also be combined, the steps can be implemented in any order, and there are many other variations of the different aspects of the invention as described above, which are not provided in detail for the sake of brevity.

Claims

1. A feed additive made from a traditional Chinese medicine extract for treating fowl dysentery, characterized in that, The raw materials for preparation include the following components in parts by weight: 10-15 parts of Scutellaria baicalensis, 6-9 parts of Coptis chinensis, 4-6 parts of stir-fried Sanguisorba officinalis, 3-5 parts of Portulaca oleracea, 2-3 parts of Moringa oleifera leaves, 1-2 parts of Citrus aurantium, and 1-2 parts of Pueraria lobata.

2. A method for preparing a feed additive of a traditional Chinese medicine extract for treating fowl dysentery as described in claim 1, characterized in that, Includes the following steps: S1. Wash and dry the Scutellaria baicalensis, Coptis chinensis, stir-fried Sanguisorba officinalis, Portulaca oleracea, Moringa oleifera leaves, Citrus aurantium, and Pueraria lobata, pulverize them, mix them evenly to obtain mixed Chinese herbal powder. Add the mixed Chinese herbal powder to boiling water, keep it warm and soak it in hot water, reflux to extract it, filter it, add the residue to boiling water again, reflux to extract it, filter it, combine the two filtrates to obtain Chinese herbal extract. S2. Take the Chinese herbal extract obtained in step S1, sterilize, cool, inoculate with activated Lactobacillus paracasei and Bifidobacterium animalis, ferment at 37°C, centrifuge, filter the supernatant, concentrate under reduced pressure, freeze dry, and obtain the Chinese herbal fermentation agent; S3. Wash and dry the Sargassum fusiforme, pulverize it to obtain Sargassum fusiforme powder, add the Sargassum fusiforme powder to 95% ethanol, reflux to defatt it, filter and collect the residue, dry it to obtain defatted Sargassum fusiforme powder, add the defatted Sargassum fusiforme powder to deionized water, sonicate, extract at 100℃, and centrifuge. S4. After centrifugation in step S3, take the supernatant, concentrate it under vacuum, add anhydrous ethanol, let it stand at 4°C, centrifuge, take the precipitate, dry it under vacuum, reconstitute it with water, freeze dry it, and mix the freeze-dried product with the traditional Chinese medicine fermentation agent obtained in step S2 at a mass ratio of 1:5 to obtain a feed additive of traditional Chinese medicine extract for treating chicken white diarrhea.

3. The method for preparing the feed additive of traditional Chinese medicine extract for treating fowl scours according to claim 2, characterized in that, In step S1, the ratio of the mixed Chinese herbal powder to boiling water is 1 g: 10 mL.

4. The method for preparing the feed additive of traditional Chinese medicine extract for treating fowl scours according to claim 3, characterized in that, In step S2, the Lactobacillus paracasei and Bifidobacterium animalis are inoculated at volume fractions of 2% and 3%, respectively.

5. The method for preparing the feed additive of traditional Chinese medicine extract for treating fowl scours according to claim 4, characterized in that, In step S2, the viable count of both *Lactobacillus paracasei* and *Bifidobacterium animalis* is 5 × 10⁻⁶. 8 CFU / mL.

6. The method for preparing the feed additive of traditional Chinese medicine extract for treating fowl scours according to claim 5, characterized in that, In step S3, the ratio of Sargassum powder to 95% ethanol is 1 g: 4-5 mL.

7. The method for preparing the feed additive of traditional Chinese medicine extract for treating fowl scours according to claim 6, characterized in that, In step S3, the ratio of defatted Sargassum powder to deionized water is 1 g: 50 mL.

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