A biological fertilizer prepared based on blue-green algae sludge

Abstract

The application provides a kind of biological fertilizer prepared based on blue-green algae mud, and belongs to the technical field of biological fertilizer preparation.The biological fertilizer according to the application comprises, by weight fraction, 20-40 parts of blue-green algae mud fermentation liquor, 30-40 parts of straw, 18-36 parts of animal manure, 5-8 parts of urea, 6-12 parts of ammonium chloride and 0.2-1 part of yeast, wherein the blue-green algae mud fermentation liquor is prepared by mixing blue-green algae mud and heat condensate, then adding enzyme preparation, mixed Bacillus and inorganic salt mixture, and anaerobic fermentation culture.The application not only realizes the resource treatment of blue-green algae mud and reduces environmental pollution, but also the prepared biological fertilizer contains nutrients such as nitrogen, phosphorus and potassium required for plant growth, and the crops applying the biological fertilizer prepared by the application can not only improve the survival rate, but also further improve the yield per mu, improve the soil quality, and the soil porosity reaches 60-65%, without caking and residue.

Description

Technical Field

[0001] This invention belongs to the field of bio-fertilizer preparation technology, and particularly relates to a bio-fertilizer prepared based on cyanobacteria mud. Background Technology

[0002] Blue-green algae sludge is primarily a sediment formed by cyanobacterial cells and their decomposition products. It typically forms in lakes, reservoirs, and other water bodies due to the death and decomposition of cyanobacteria after their massive proliferation. Direct use or improper treatment of blue-green algae sludge can cause secondary pollution to soil and water bodies, impacting the ecological environment. The accumulation of blue-green algae sludge in water bodies can lead to water quality deterioration, affecting aquatic biodiversity and the health of aquatic ecosystems. Furthermore, the decomposition of cyanobacteria may release algal toxins, which pose a direct threat to human health. To mitigate these potential hazards, some cities and regions are exploring diversified disposal methods for blue-green algae sludge, such as mixing it with household waste and then incinerating it at high temperatures, to achieve harmless treatment and resource utilization. However, current traditional methods for treating cyanobacteria toxins easily cause secondary environmental pollution. Therefore, the general method for treating cyanobacteria involves using significant manpower and resources to directly remove the algae, failing to address the issue of resource utilization.

[0003] While using cyanobacteria mud to prepare bio-fertilizer has certain potential for resource utilization, it also has some drawbacks: the nutritional components of cyanobacteria mud may not be fully matched with the nutrients required by crops, requiring further processing to meet the needs of plant growth; if cyanobacteria mud contains incompletely decomposed organic matter and nutrients, direct application to the soil may cause secondary pollution.

[0004] Therefore, this invention proposes a bio-fertilizer based on cyanobacterial mud to realize the resource utilization of cyanobacterial resources. Summary of the Invention

[0005] To address the aforementioned technical problems, this invention proposes a bio-fertilizer based on cyanobacteria sludge.

[0006] To achieve the above objectives, the present invention provides a bio-fertilizer based on cyanobacteria mud, comprising, by weight: 20-40 parts of cyanobacteria mud fermentation liquid, 30-40 parts of straw, 18-36 parts of animal manure, 5-8 parts of urea, 6-12 parts of ammonium chloride and 0.2-1 parts of yeast. The cyanobacterial mud fermentation liquid is prepared by mixing cyanobacterial mud and thermal coagulation, then adding enzyme preparations, mixed Bacillus and inorganic salts, and anaerobic fermentation culture.

[0007] The thermal coagulant used is a coagulated product of proteins and other organic matter formed during the boiling stage of beer production.

[0008] Furthermore, the mass ratio of cyanobacterial mud to thermal coagulation in the cyanobacterial mud fermentation liquid is (10-15):5, the amount of enzyme preparation added is 2% of the weight of cyanobacterial mud, the amount of mixed Bacillus added is 6% of the weight of cyanobacterial mud, and the amount of inorganic salt added is 2% of the weight of cyanobacterial mud.

[0009] Furthermore, the enzyme preparation includes pectinase, cellulase, and saccharifying enzyme.

[0010] Furthermore, the enzyme preparation comprises pectinase, cellulase, and saccharifying enzyme in a mass ratio of 1:1:4.

[0011] Furthermore, the mixed Bacillus species includes Bacillus subtilis and Bacillus mucilaginosa.

[0012] Furthermore, the mixed Bacillus comprises Bacillus subtilis and Bacillus mucilaginosa in a mass ratio of 1:1.

[0013] Furthermore, the inorganic salts include magnesium sulfate and potassium dihydrogen phosphate.

[0014] Furthermore, the inorganic salt comprises magnesium sulfate and potassium dihydrogen phosphate in a mass ratio of 1:3.

[0015] Furthermore, the fermentation culture temperature is 30-35℃, and the fermentation time is 3-5 days.

[0016] Furthermore, by weight, it includes: 38 parts of blue-green algae fermentation liquid, 36 parts of straw, 20 parts of animal manure, 6 parts of urea, 10 parts of ammonium chloride, and 0.3 parts of yeast.

[0017] This invention also provides a method for preparing the bio-fertilizer based on cyanobacteria mud, comprising the following steps: Weigh each raw material according to the specified weight proportions, mix the blue-green algae mud fermentation liquid, straw, animal manure and yeast evenly, and then ferment. After fermentation, dehydrate and mix with urea and ammonium chloride to obtain the bio-fertilizer prepared based on blue-green algae mud.

[0018] Furthermore, the fermentation temperature is 35-45℃, and the fermentation time is 6-7 days.

[0019] Furthermore, the dehydration is performed until the moisture content is less than 30%.

[0020] Compared with the prior art, the present invention has the following advantages and technical effects: (1) In this invention, cyanobacteria mud is pretreated with enzyme preparations, Bacillus, etc., and then sealed and fermented with thermal coagulation. The enzyme preparations and microorganisms are used to eliminate cyanobacterial toxins in cyanobacteria. Then, it is mixed with straw, animal manure, urea, etc. for fermentation to prepare bio-organic fertilizer.

[0021] (2) The bio-fertilizer of the present invention is rich in cyanobacterial mud fermentation liquid prepared by mixing thermal coagulant and cyanobacterial mud. By mixing beer thermal coagulant and cyanobacterial mud in a certain proportion and carrying out anaerobic nitrification treatment, the organic matter in the thermal coagulant can be converted into biogas, and at the same time, nitrification residue rich in microorganisms and nutrients is produced. This nitrification residue can be used as bio-fertilizer. The microorganisms and nutrients it contains can promote plant growth and improve soil fertility and structure. The cyanobacterial mud can adsorb nutrients in the thermal coagulant, provide a growth environment for microorganisms, and increase microbial activity. At the same time, the porous structure of the cyanobacterial mud has a slow-release effect, which helps to improve soil permeability and water retention and promote plant root development.

[0022] (3) This invention not only realizes the resource utilization of blue-green algae mud and reduces environmental pollution, but also produces bio-fertilizer containing nutrients such as nitrogen, phosphorus and potassium required for plant growth. Compared with unused crops, crops using the bio-fertilizer prepared by this invention can not only improve the survival rate, but also further increase the yield per mu, improve the soil quality, and achieve a soil porosity of 60-65%, without clumping or residue. Detailed Implementation

[0023] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0024] It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the invention. Furthermore, with respect to numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Every smaller range between any stated value or intermediate value within a stated range, and any other stated value or intermediate value within said range, is also included in this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0025] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.

[0026] Various modifications and variations can be made to the specific embodiments described in this specification without departing from the scope or spirit of the invention, as will be apparent to those skilled in the art. Other embodiments derived from this specification will also be readily apparent to those skilled in the art. This specification and embodiments are merely exemplary.

[0027] The terms “include,” “including,” “have,” “contain,” etc., used in this article are all open-ended terms, meaning that they include but are not limited to.

[0028] This invention provides a bio-fertilizer based on cyanobacteria mud, comprising, by weight: 20-40 parts cyanobacteria mud fermentation liquid, 30-40 parts straw, 18-36 parts animal manure, 5-8 parts urea, 6-12 parts ammonium chloride, and 0.2-1 parts yeast. The cyanobacterial mud fermentation liquid is prepared by mixing cyanobacterial mud and thermal coagulation, then adding enzyme preparations, mixed Bacillus and inorganic salts, and anaerobic fermentation culture.

[0029] In this embodiment of the invention, the thermal coagulant used is a coagulated product of proteins and other organic matter formed during the boiling stage of beer production. Specifically, it comes from a brewery in Heilongjiang Province, and the blue-green algae mud used is collected from Baishi Reservoir.

[0030] In a preferred embodiment of the present invention, the mass ratio of cyanobacterial mud to thermal coagulation in the cyanobacterial mud fermentation liquid is (10-15):5, the amount of enzyme preparation added is 2% of the weight of cyanobacterial mud, the amount of mixed Bacillus added is 6% of the weight of cyanobacterial mud, and the amount of inorganic salt added is 2% of the weight of cyanobacterial mud.

[0031] In a preferred embodiment of the present invention, the enzyme preparation includes pectinase, cellulase and saccharifying enzyme; more preferably, the enzyme preparation includes pectinase, cellulase and saccharifying enzyme in a mass ratio of 1:1:4.

[0032] By adding enzyme preparations, residual nutrients in waste diatomaceous earth can be decomposed and released, serving as one of the nutrients for crop growth in bio-fertilizer. The enzyme preparations used in this embodiment of the invention were all purchased from Jinan Bestjie Bioengineering Co., Ltd., with pectinase having an activity of 20,000 U / g, cellulase having an activity of 10,000 U / g, and saccharifying enzyme having an activity of 100,000 U / g.

[0033] In a preferred embodiment of the present invention, the mixed Bacillus includes Bacillus subtilis and Bacillus mucilaginosus. More preferably, the mixed Bacillus includes Bacillus subtilis and Bacillus mucilaginosus in a mass ratio of 1:1.

[0034] The Bacillus subtilis used in the embodiments of the present invention was purchased from Shanghai Yaji Biotechnology Co., Ltd., the Bacillus jellyoidus used was purchased from Wuhan Huanna Biotechnology Co., Ltd., and the yeast used was purchased from Ningbo Mingzhou Biotechnology Co., Ltd.

[0035] In a preferred embodiment of the present invention, the inorganic salt comprises magnesium sulfate and potassium dihydrogen phosphate. More preferably, the inorganic salt comprises magnesium sulfate and potassium dihydrogen phosphate in a mass ratio of 1:3.

[0036] In a preferred embodiment of the present invention, the fermentation temperature is 30-35℃ and the fermentation time is 3-5 days.

[0037] In a preferred embodiment of the present invention, the mixture comprises, by weight, 38 parts of blue-green algae fermentation liquid, 36 parts of straw, 20 parts of animal manure, 6 parts of urea, 10 parts of ammonium chloride, and 0.3 parts of yeast.

[0038] This invention also provides a method for preparing the bio-fertilizer based on cyanobacteria mud, comprising the following steps: Weigh each raw material according to the specified weight proportions, mix the blue-green algae mud fermentation liquid, straw, animal manure and yeast evenly, and then ferment. After fermentation, dehydrate and mix with urea and ammonium chloride to obtain the bio-fertilizer prepared based on blue-green algae mud.

[0039] In a preferred embodiment of the present invention, the fermentation temperature is 35-45°C and the fermentation time is 6-7 days.

[0040] In a preferred embodiment of the present invention, the dehydration is performed until the moisture content is less than 30%.

[0041] In the embodiments of this invention, unless otherwise specified, "parts" refers to "number of parts by weight".

[0042] The technical solution of the present invention will be further illustrated by the following embodiments.

[0043] Example 1 (1) Preparation of cyanobacterial mud fermentation liquid The cyanobacterial sludge and thermal coagulation were mixed at a mass ratio of 12:5, and NaOH was added to adjust the pH to 7.2. Then, enzyme preparations (pectinase, cellulase and saccharifying enzyme in a mass ratio of 1:1:4, added at 2% of the weight of the cyanobacterial sludge), mixed Bacillus (Bacillus subtilis and Bacillus mucilaginosus in a mass ratio of 1:1, added at 6% of the weight of the cyanobacterial sludge) and inorganic salts (magnesium sulfate and potassium dihydrogen phosphate in a mass ratio of 1:3, added at 2% of the weight of the cyanobacterial sludge) were added and mixed. The mixture was then anaerobic fermented at 34℃ for 4 days to prepare the product. (2) Preparation of bio-fertilizer based on cyanobacteria mud Weigh out 38 parts of the cyanobacterial mud fermentation liquid, 36 parts of straw, 20 parts of animal manure, 6 parts of urea, 10 parts of ammonium chloride and 0.3 parts of yeast according to the weight proportions in step (1). Mix the cyanobacterial mud fermentation liquid, straw, animal manure and yeast evenly, and ferment at 38℃ for 6 days. After fermentation, dehydrate to a moisture content of less than 30%, and mix with urea and ammonium chloride to obtain bio-fertilizer based on cyanobacterial mud.

[0044] Example 2 (1) Preparation of cyanobacterial mud fermentation liquid The cyanobacterial sludge and thermal coagulation were mixed in a ratio of 15:5, and NaOH was added to adjust the pH to 7.4. Then, enzyme preparations (pectinase, cellulase and saccharifying enzyme in a mass ratio of 1:1:4, added at 2% of the weight of the cyanobacterial sludge), mixed Bacillus (Bacillus subtilis and Bacillus mucilaginosus in a mass ratio of 1:1, added at 6% of the weight of the cyanobacterial sludge) and inorganic salts (magnesium sulfate and potassium dihydrogen phosphate in a mass ratio of 1:3, added at 2% of the weight of the cyanobacterial sludge) were added and mixed. The mixture was then anaerobic fermented at 35°C for 3 days to prepare the product. (2) Preparation of bio-fertilizer based on cyanobacteria mud Weigh out 40 parts of the cyanobacterial mud fermentation liquid, 30 parts of straw, 36 parts of animal manure, 5 parts of urea, 6 parts of ammonium chloride and 1 part of yeast according to the weight proportions in step (1). Mix the cyanobacterial mud fermentation liquid, straw, animal manure and yeast evenly, and ferment at 45℃ for 6 days. After fermentation, dehydrate to a moisture content of less than 30%, and mix with urea and ammonium chloride to obtain bio-fertilizer based on cyanobacterial mud.

[0045] Example 3 (1) Preparation of cyanobacterial mud fermentation liquid The cyanobacterial mud and thermal coagulated material were mixed in a ratio of 10:5, and NaOH was added to adjust the pH to 7. Then, enzyme preparations (pectinase, cellulase and saccharifying enzyme in a mass ratio of 1:1:4, added at 2% of the weight of the cyanobacterial mud), mixed Bacillus (Bacillus subtilis and Bacillus mucilaginosus in a mass ratio of 1:1, added at 6% of the weight of the cyanobacterial mud) and inorganic salts (magnesium sulfate and potassium dihydrogen phosphate in a mass ratio of 1:3, added at 2% of the weight of the cyanobacterial mud) were added and mixed. The mixture was then anaerobic fermented at 30°C for 5 days to prepare the product. (2) Preparation of bio-fertilizer based on cyanobacteria mud Weigh out 20 parts of the cyanobacterial mud fermentation liquid, 40 parts of straw, 18 parts of animal manure, 8 parts of urea, 12 parts of ammonium chloride and 0.2 parts of yeast according to the weight proportions in step (1). Mix the cyanobacterial mud fermentation liquid, straw, animal manure and yeast evenly, and ferment at 45℃ for 7 days. After fermentation, dehydrate to a moisture content of less than 30%, and mix with urea and ammonium chloride to obtain bio-fertilizer based on cyanobacterial mud.

[0046] Comparative Example 1 Same as Example 1, except that the cyanobacteria fermentation liquid is prepared according to the following method: The cyanobacterial mud was prepared by adding NaOH to adjust the pH to 7.2, then adding enzyme preparations (pectinase, cellulase and saccharifying enzyme in a mass ratio of 1:1:4, added at 2% of the weight of the cyanobacterial mud), mixed Bacillus (Bacillus subtilis and Bacillus mucilaginosus in a mass ratio of 1:1, added at 6% of the weight of the cyanobacterial mud), and inorganic salts (magnesium sulfate and potassium dihydrogen phosphate in a mass ratio of 1:3, added at 2% of the weight of the cyanobacterial mud). The mixture was then anaerobic fermented at 34℃ for 4 days. Step (2) is the same as in Example 1.

[0047] Comparative Example 2 Same as Example 1, except that the cyanobacteria fermentation liquid is prepared according to the following method: The cyanobacterial sludge and thermal coagulation were mixed in a ratio of 12:5, and NaOH was added to adjust the pH to 7.2. Then, mixed Bacillus subtilis and Bacillus mucilaginosus in a 1:1 mass ratio, added at 6% of the weight of the cyanobacterial sludge, and inorganic salts (magnesium sulfate and potassium dihydrogen phosphate in a 1:3 mass ratio, added at 2% of the weight of the cyanobacterial sludge) were added and mixed. The mixture was then anaerobic fermented at 34℃ for 4 days to prepare the product. Step (2) is the same as in Example 1.

[0048] Comparative Example 3 Same as Example 1, except that the cyanobacteria fermentation liquid is prepared according to the following method: The cyanobacterial sludge and thermal coagulation were mixed in a ratio of 12:5, and NaOH was added to adjust the pH to 7.2. Then, enzyme preparations (pectinase, cellulase and saccharifying enzyme in a mass ratio of 1:1:4, added at 2% of the weight of the cyanobacterial sludge) and inorganic salts (magnesium sulfate and potassium dihydrogen phosphate in a mass ratio of 1:3, added at 2% of the weight of the cyanobacterial sludge) were added and mixed. The mixture was then anaerobic fermented at 34℃ for 4 days to prepare the product. Step (2) is the same as in Example 1.

[0049] Comparative Example 4 Same as Example 1, except that in step (2), 60 parts of blue algae mud fermentation liquid, 15 parts of straw, 10 parts of animal manure, 15 parts of urea, 5 parts of ammonium chloride and 0.1 parts of yeast are weighed according to the following weight proportions.

[0050] Comparative Example 5 Same as Example 1, except that in step (2), 38 parts of the cyanobacterial mud fermentation liquid, 36 parts of straw, 20 parts of animal manure, 6 parts of urea, and 10 parts of ammonium chloride prepared in step (1) are weighed according to the weight proportions. After the cyanobacterial mud fermentation liquid, straw, animal manure, urea and ammonium chloride are mixed evenly, a bio-fertilizer based on cyanobacterial mud is obtained.

[0051] Application effect experiment To further illustrate the advantages of the bio-fertilizer based on cyanobacteria sludge produced in the embodiments of the present invention, a peanut planting experiment was conducted in a peanut field in Heishan County, Liaoning Province, and the experimental results, including the peanut yield increase effect, were statistically analyzed. The experimental details are as follows: Treatment groups 1-3: The bio-fertilizers prepared based on cyanobacteria mud as described in Examples 1-3 were applied respectively, with an application rate of 100 kg / mu. Treatment groups 4-8: Bio-fertilizers based on cyanobacterial mud prepared in comparative examples 1-5 were applied at a rate of 100 kg / mu. Control group 1: Apply conventional peanut fertilizer - use 15-15-15 compound fertilizer, the application rate is 100 kg / mu; All other peanut field management measures were the same for each treatment group and the control group.

[0052] The experimental results are shown in Table 1.

[0053] Table 1. Effects of different treatments on peanut yield and pests and diseases. As can be seen from Table 1, the application of the bio-fertilizer prepared in the embodiments of the present invention can increase yield and income. Compared with conventional fertilizer (control group 1), peanut yield increased by 108-113 kg per mu, and the yield increase effect was obvious. In addition, the application of the bio-fertilizer of the present invention can effectively inhibit the occurrence of peanut diseases and pests.

[0054] After peanut harvest, soil bulk density, specific gravity, and porosity were measured in each treatment group and control group. The results are shown in Table 2.

[0055] Soil bulk density was determined according to the method in NY / T 1121.4-2006, and soil specific gravity and porosity were determined according to the ring sampler method.

[0056] Table 2. Effects of different treatments on soil bulk density, specific gravity, and porosity. As shown in Table 2, the application of the bio-fertilizer prepared in the embodiments of the present invention has a significant impact on soil bulk density, specific gravity, and porosity. It can reduce soil bulk density and specific gravity, increase soil porosity, and make the soil in peanut fields significantly looser and enhance the soil's ability to supply fertilizer, thereby improving the peanut's absorption of fertilizer and increasing its yield.

[0057] The above are merely preferred embodiments of this application, but the scope of protection of this application is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in this application should be included within the scope of protection of this application. Therefore, the scope of protection of this application should be determined by the scope of the claims.

Claims

1. A bio-fertilizer prepared based on cyanobacteria sludge, characterized in that, By weight, it includes: 20-40 parts blue-green algae mud fermentation liquid, 30-40 parts straw, 18-36 parts animal manure, 5-8 parts urea, 6-12 parts ammonium chloride and 0.2-1 parts yeast; The cyanobacterial mud fermentation liquid is prepared by mixing cyanobacterial mud and thermal coagulation, then adding enzyme preparations, mixed Bacillus and inorganic salts, and anaerobic fermentation culture.

2. The bio-fertilizer prepared based on cyanobacteria sludge according to claim 1, characterized in that, The mass ratio of cyanobacterial mud to thermal coagulation in the cyanobacterial mud fermentation liquid is (10-15):

5. The amount of enzyme preparation added is 2% of the weight of cyanobacterial mud, the amount of mixed Bacillus added is 6% of the weight of cyanobacterial mud, and the amount of inorganic salt added is 2% of the weight of cyanobacterial mud.

3. The bio-fertilizer prepared based on cyanobacteria sludge according to claim 2, characterized in that, The enzyme preparation includes pectinase, cellulase, and saccharifying enzyme.

4. The bio-fertilizer prepared based on cyanobacteria sludge according to claim 2, characterized in that, The mixed Bacillus species includes Bacillus subtilis and Bacillus mucilaginosa.

5. The bio-fertilizer prepared based on cyanobacteria sludge according to claim 2, characterized in that, The inorganic salts include magnesium sulfate and potassium dihydrogen phosphate.

6. The bio-fertilizer prepared based on cyanobacteria sludge according to claim 1, characterized in that, The fermentation culture temperature is 30-35℃, and the fermentation time is 3-5 days.

7. The bio-fertilizer prepared based on cyanobacteria sludge according to claim 1, characterized in that, The ingredients, by weight, include: 38 parts blue-green algae fermentation liquid, 36 parts straw, 20 parts animal manure, 6 parts urea, 10 parts ammonium chloride, and 0.3 parts yeast.

8. A method for preparing the bio-fertilizer based on cyanobacteria sludge as described in any one of claims 1-7, characterized in that, Includes the following steps: Weigh each raw material according to the specified weight proportions, mix the blue-green algae mud fermentation liquid, straw, animal manure and yeast evenly, and then ferment. After fermentation, dehydrate and mix with urea and ammonium chloride to obtain the bio-fertilizer prepared based on blue-green algae mud.

9. The method for preparing bio-fertilizer based on cyanobacteria mud according to claim 8, characterized in that, The fermentation temperature is 35-45℃, and the fermentation time is 6-7 days.

10. The method for preparing bio-fertilizer based on cyanobacteria mud according to claim 8, characterized in that, The dehydration is performed until the moisture content is less than 30%.

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