Thunberg fritillary bulb advanced embryonic development abundant protein FtLEA14, coding gene and application

By cloning the coding sequence of FtLEA14, a protein from the late embryonic development stage of Fritillaria thunbergii, and overexpressing the FtLEA14 gene, the problem of drought affecting the quality and yield of Fritillaria thunbergii was solved, and the drought resistance of Fritillaria thunbergii was improved.

CN122103296AActive Publication Date: 2026-05-29ZHEJIANG FORESTRY UNIVERSITY
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Patent Information

Application Number
CN202610573698.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-04-28
Publication Date
2026-05-29
Estimated Expiration
2046-04-28

AI Technical Summary

Technical Problem

Fritillaria thunbergii often encounters seasonal drought during its growth period, which affects the quality and yield of the medicinal material. Current technology has not been able to effectively utilize the function of LEA protein in Fritillaria thunbergii.

Method used

The coding sequence of FtLEA14, a protein abundant in the late embryonic development of Fritillaria thunbergii, was cloned. By overexpressing the FtLEA14 gene and using the recombinant expression vector pHB-FtLEA14-YFP, the leaf water loss rate was reduced, thereby improving the plant's drought stress resistance.

Benefits of technology

It significantly reduced the rate of water loss from the leaves, improved the drought resistance of Fritillaria thunbergii, ensured the yield and quality of the medicinal material, and provided a theoretical basis for the application of genetic engineering technology in Fritillaria thunbergii.

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Abstract

The invention discloses a thunberg fritillary bulb advanced embryonic development abundant protein FtLEA14, a coding gene and application, the amino acid sequence of the advanced embryonic development abundant protein FtLEA14 is shown as SEQ ID NO.2, the nucleotide sequence of the coding gene is shown as SEQ ID NO.1, and the nucleotide sequence of the coding gene is shown as SEQ ID NO.2. The invention also provides an application of the encoding gene of the thunberg fritillary bulb advanced embryonic development abundant protein FtLEA14 in improving the drought resistance of the thunberg fritillary bulb. The invention provides a theoretical basis for promoting the improvement of the drought stress resistance of the thunberg fritillary bulb by utilizing a genetic engineering technology, and has important application value.
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