Black sheep 40K liquid phase chip based on single nucleotide polymorphism and application of black sheep 40K liquid phase chip

By developing a 40K liquid phase chip for breeding Turpan black sheep, the problem of low coverage of existing sheep chips has been solved, which has improved the accuracy and efficiency of Turpan black sheep breeding and protected its unique genetic resources.

CN122104932APending Publication Date: 2026-05-29XINJIANG ACAD OF ANIMAL SCI
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Patent Information

Application Number
CN202610163406.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-02-05
Publication Date
2026-05-29

AI Technical Summary

Technical Problem

Existing sheep microchips have low coverage of specific functional sites for Turpan black sheep, resulting in low breeding efficiency, difficulty in ensuring breed purity, and inability to meet the needs of large-scale breeding.

Method used

A 40K liquid-phase chip for breeding Turpan black sheep was developed. By constructing a 40K SNP locus set for genotyping of Turpan black sheep, probe combinations were designed and probe kits were prepared. Genotyping was performed using the NGP liquid-phase chip system, and combined with bioinformatics analysis, genomic selection and kinship identification were achieved.

Benefits of technology

This has improved the precision and efficiency of breeding, shortened the breeding cycle, protected the unique genetic resources of Turpan black sheep, and enhanced the accuracy and economic value of breeding.

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Abstract

The invention relates to the field of gene molecular breeding, and discloses a single nucleotide polymorphism-based black sheep 40K liquid phase chip and application thereof. The preparation method of the chip comprises the following steps: acquiring phenotype data of Turpan black sheep, extracting DNA from collected blood, and sequencing; sequencing is conducted through a DNA library, obtained original data are compared with a sheep reference genome after quality control, sequencing is conducted after comparison is completed, and BAM data are obtained; carrying out variation detection on the BAM data of all samples to obtain all SNP loci, and then carrying out quality control; carrying out screening of chip background SNP sites and screening of variety-specific SNP sites by utilizing the SNP data subjected to quality control; and combining the selected chip background SNP sites with the variety-specific SNP sites, and designing a probe to manufacture a liquid phase chip. According to the chip, upstream and downstream SNP marker information of a target site can be utilized to the maximum extent, so that the breeding precision and efficiency are improved, and directional improvement of the production performance of Turpan black sheep is promoted.
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