Tomato SlRRB9 gene and application of knockout vector of tomato SlRRB9 gene in tomato
By knocking out the SlRRB9 gene, a tomato response regulator, using CRISPR/Cas9 gene editing technology, the problem of regulating tomato fruit size was solved, achieving the breeding goals of larger fruit and higher yield, while reducing breeding costs.
CN122146777AActive Publication Date: 2026-06-05NANJING AGRICULTURAL UNIVERSITY
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Patent Information
- Application Number
- CN202610629278.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-05-09
- Publication Date
- 2026-06-05
- Estimated Expiration
- 2046-05-09
AI Technical Summary
Technical Problem
Existing technologies are insufficient to effectively control tomato fruit size, which affects fruit enlargement and breeding efficiency.
Method used
The SlRRB9 response regulator gene in tomato was knocked out using CRISPR/Cas9 gene editing technology. The target sgRNA sequence was designed and the CRISPR/Cas9 gene editing vector pYLCRISPRCas9Pubi-N-SlRRB9 was constructed and introduced into tomato for genetic transformation.
Benefits of technology
It significantly increases the size and weight of tomato fruits, promotes high yield and reduces breeding costs.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention provides a tomato SlRRB9 gene and application of a knockout vector of the tomato SlRRB9 gene in tomatoes. The gene number of the tomato response regulator gene SlRRB9 is LOC101258668, and the nucleotide sequence of the tomato response regulator gene SlRRB9 is as shown in SEQ ID NO. 1. The invention discloses a knockout vector of a tomato response regulator gene SlRRB9 and an engineering bacterium comprising the knockout vector. The knockout vector targets an sgRNA-1 sequence as shown in SEQ ID NO. 2 and an sgRNA-2 sequence as shown in SEQ ID NO. 3. The application is selected from 1) and / or 2): 1) increasing the size of tomato fruits and 2) increasing the weight of the tomato fruits. According to the invention, tomato plants with increased fruits can be obtained by editing the SlRRB9 gene through the CRISPR / Cas9 gene, which is of great significance in promoting high-quality and high-yield tomato and reducing the breeding cost.
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