Sesame peptide and application thereof

Abstract

The present application relates to a kind of sesame peptide and its application, the preparation method of sesame peptide includes the following steps: (one) with the pressed sesame meal as raw material, sesame meal is pretreated;(two) using first enzyme group, the product after pretreatment is removed impurity;(three) the enzyme solution after the first enzyme group enzymolysis is treated with protein extraction;(four) using second enzyme group, the sesame protein in the product after protein extraction treatment is enzymolysis;First enzyme group is composed of pectinase, cellulase, lipase and high-temperature amylase;Second enzyme group is composed of alkaline protease, neutral protease and flavour protease.Compared with prior art, the sesame peptide in the present application has high protein content, and can efficiently release arginine in sesame meal, wherein the content of arginine is greatly improved, and can well play the effect of improving male sexual function impairment.

Description

Technical Field

[0001] This invention relates to the field of biotechnology, and more particularly to a sesame peptide and its applications. Background Technology

[0002] Against the backdrop of increasing societal, work, and family pressures, erectile dysfunction and prostate-related diseases are becoming increasingly prevalent among men, significantly disrupting their daily lives. Penile erection, a complex physiological phenomenon, is achieved through the coordinated efforts of multiple bodily systems under sexual stimulation. This process involves changes in hemodynamics, precise neuroendocrine regulation, and the influence of psychological state, among other factors. Erectile dysfunction is specifically characterized by the inability to achieve or maintain an erection of sufficient rigidity for more than three months, resulting in unsatisfactory sexual intercourse. Those troubled by this problem not only experience disruptions to their normal sex life but also suffer from fluctuating emotional states, leading to a decline in their overall quality of life. Furthermore, the incidence and severity of erectile dysfunction increase with age.

[0003] Arginine plays a vital role in male reproductive health. It participates in spermatogenesis, enhances sperm motility, promotes the synthesis of the signaling molecule cGMP, and helps delay lipid peroxidation of sperm cell membranes. Supplementing with arginine or consuming arginine-rich proteins and peptides can, to some extent, improve the condition of the reproductive system, thereby enhancing male fertility and sexual function. Although the bioavailability of arginine monomers is limited, arginine-containing peptides are considered a more ideal form of arginine supplementation due to their better water solubility, lower risk of allergic reactions, good absorption, and strong stability.

[0004] Sesame (Sesamum indicum L.), also known as sesame or flaxseed, is an annual herbaceous plant belonging to the Sesamaceae family and the Sesamum genus. Sesame has an oil content as high as 55%, making it an important source of edible oil in my country. The extracted oil is called sesame oil, flaxseed oil, or fragrant oil, characterized by its rich aroma and high nutritional value. Sesame meal is a byproduct of sesame oil extraction, mainly composed of sesame protein (such as albumin and globulin), residual oil, and lignans. Arginine is an important component of sesame protein; during seed development, arginine is integrated into polypeptide chains and stored in protein bodies as proteins.

[0005] Enzymatic hydrolysis of proteins refers to the process of using enzymes to degrade large protein molecules into smaller peptides and even amino acids. As an efficient, mild, safe, and easily controlled processing method, enzymatic hydrolysis technology is one of the key approaches to achieving protein functional diversification and enhancing its application value. Sesame protein can be enzymatically hydrolyzed to form sesame peptides containing free arginine. However, sesame peptides prepared from sesame meal using existing methods generally suffer from low protein and arginine content. Summary of the Invention

[0006] The first technical problem to be solved by the present invention is to provide a sesame peptide with high protein content and high arginine content, in light of the current state of the technology.

[0007] The second technical problem to be solved by the present invention is to provide an application of the above-mentioned sesame peptide in light of the current state of the technology.

[0008] The technical solution adopted by the present invention to solve the first technical problem mentioned above is: a sesame peptide, characterized in that the preparation method of the sesame peptide includes the following steps:

[0009] (a) Using pressed sesame meal as raw material, pre-process the sesame meal;

[0010] (ii) Use the first enzyme group to remove impurities from the pretreated product;

[0011] (iii) Protein extraction treatment of the enzymatic hydrolysate after enzymatic hydrolysis by the first enzyme group;

[0012] (iv) Enzymatic hydrolysis of sesame protein in the product after protein extraction using the second enzyme group;

[0013] The first enzyme group consists of pectinase, cellulase, lipase and high-temperature amylase; the second enzyme group consists of alkaline protease, neutral protease and flavor protease.

[0014] Furthermore, in step (ii), the pretreated product is first enzymatically hydrolyzed by the combination of pectinase, cellulase and lipase, and then the hydrolysate obtained from the first enzymatic hydrolysis is hydrolyzed by the high-temperature amylase.

[0015] Furthermore, the mass ratio of the pectinase, cellulase, lipase, and high-temperature amylase is 1:2:1:1.

[0016] Furthermore, in step (iii), the alkaline protease, neutral protease, and flavor protease sequentially hydrolyze the sesame protein.

[0017] Furthermore, the mass ratio of the alkaline protease, neutral protease, and flavor protease is 2:1:1.

[0018] Furthermore, the preprocessing in step (a) includes the following operations:

[0019] (a) Sieve the sesame meal; (b) Soak the sieved sesame meal in water; (c) Acid wash the precipitate obtained from the soaking.

[0020] Furthermore, step (iii) involves using alkaline extraction and acid precipitation techniques to extract protein.

[0021] Furthermore, in the alkaline extraction and acid precipitation technology, the pH of the alkaline extraction is 10.0, while the pH of the acid precipitation is 3.7.

[0022] Furthermore, the sesame peptide is prepared through the following steps:

[0023] (1) Sieving: Take sesame meal, pass it through a 50-mesh sieve, and set aside;

[0024] (2) Water soaking: Add purified water to the sieved sesame meal, treat at 90℃ for 1 hour, centrifuge at 4000 rpm for 10-15 minutes, and collect the precipitate;

[0025] (3) Enzymatic hydrolysis: Add purified water to the collected precipitate, and add three enzymes according to the following dosage: 1% pectinase, 2% cellulase, and 1% lipase by weight of the precipitate. Maintain the temperature at 50°C and the pH at 4.5-5.5 for 1 hour. Then, raise the temperature of the enzymatic hydrolysate system to 80°C, adjust the pH to 7.0, and add high-temperature amylase according to the following dosage: 1% by weight of the precipitate. Treat for 1 hour.

[0026] (4) Alkali extraction: Centrifuge at 5000 rpm for 10 min to collect the precipitate, add 3 L of purified water to the precipitate, adjust the pH to 10, and treat for 1 h;

[0027] (5) Precipitation with acid: Centrifuge again at 5000 rpm for 10 min, collect the supernatant, adjust the pH of the supernatant to 3.7, treat for 1 h, and centrifuge again to collect the precipitate;

[0028] (6) Add purified water to the above precipitate and adjust the pH value to 8.0~8.5. Add alkaline protease at a dosage of 0.2% of the weight of the above precipitate and treat at 55℃ for 1h.

[0029] (7) Adjust the pH of the above enzymatic hydrolysate to 7.0~7.5, maintain 55℃, and add neutral protease at a dosage of 0.1% of the above precipitate weight, and treat for 1h;

[0030] (8) Maintain the pH of the above enzymatic hydrolysate at 7.0~7.5, maintain the temperature at 55℃, and add flavor protease at a dosage of 0.1% of the above precipitate weight, and treat for 1h;

[0031] (9) After centrifuging the final enzymatic hydrolysate at 4000 rpm for 10-15 min, take the supernatant and desalt it using a nanofiltration membrane with a molecular weight cutoff of 300 Da. Concentrate the total volume to half of the original volume. The nanofiltration parameters are: operating pressure 0.5-1.0 MPa and temperature 20-30℃. Obtain the desired sesame peptide powder by spray drying.

[0032] The technical solution adopted to further solve the second technical problem mentioned above is: an application of sesame peptide as described above, characterized in that the sesame peptide is used in the preparation of food, health products or drugs that have the function of improving sexual dysfunction.

[0033] Compared with the prior art, the advantages of the present invention are as follows:

[0034] This invention employs a combination of pectinase, cellulase, and lipase to treat sesame meal, rapidly breaking down macromolecules such as pectin, cellulose, and fat, thus facilitating the rapid release of protein from the sesame meal. Further treatment with high-temperature amylase removes starch and improves protein purity. Alkaline protease, neutral protease, and flavor protease sequentially hydrolyze sesame protein, utilizing the diverse cleavage sites of different proteases to obtain sesame peptides with the lowest possible molecular weight, achieving efficient utilization of sesame meal.

[0035] The sesame peptide protein prepared by the preparation method provided by the present invention has a high content and can efficiently release arginine from sesame meal. The arginine content is greatly increased, which can significantly improve the levels of testosterone, dihydrotestosterone and cGMP, thereby effectively improving male sexual dysfunction. Attached Figure Description

[0036] Figure 1 The effect of sesame peptide on testosterone secreted by TM3 cells in this invention is given by ****, where **** indicates p < 0.0001 and ** indicates p < 0.01.

[0037] Figure 2 The effect of the sesame peptide of the present invention on dihydrotestosterone secreted by TM3 cells is given by ****, where **** indicates p < 0.0001, *** indicates p < 0.001, and ns indicates p > 0.05.

[0038] Figure 3 The effect of the sesame peptide of the present invention on cGMP secreted by TM3 cells is given, where **** indicates p<0.0001. Detailed Implementation

[0039] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments.

[0040] Example:

[0041] (1) Sieving: Take 1 kg of sesame meal, sieve it through a 50-mesh sieve, and set aside.

[0042] (2) Water soaking: Add 3 kg of purified water to the sieved sesame meal, treat at 90℃ for 1 h, centrifuge at 4000 rpm for 10-15 min, and collect the precipitate;

[0043] (3) Enzymatic hydrolysis: Add 3 kg of purified water to the collected precipitate, and add three enzymes according to the following dosage: 1% pectinase, 2% cellulase, and 1% lipase by weight of the precipitate. Maintain the temperature at 50 °C and the pH at 4.5-5.5 for 1 h. Then raise the temperature of the enzymatic hydrolysate system to 80 °C, adjust the pH to 7.0, and add high-temperature amylase according to the following dosage: 1% by weight of the precipitate. Treat for 1 h.

[0044] (4) Alkali extraction: Centrifuge at 5000 rpm for 10 min to collect the precipitate, add 3 L of purified water to the precipitate, adjust the pH to 10.0, and treat for 1 h;

[0045] (5) Precipitation with acid: Centrifuge again at 5000 rpm for 10 min, collect the supernatant, adjust the pH of the supernatant to 3.7, treat for 1 h, and centrifuge again to collect the precipitate;

[0046] (6) Add 3 L of purified water to the above precipitate, adjust the pH value to 8.0~8.5, add alkaline protease at a dosage of 0.2% of the weight of the above precipitate, and treat at 55℃ for 1 h;

[0047] (7) Adjust the pH of the above enzymatic hydrolysate to 7.0~7.5, maintain 55℃, and add neutral protease at a dosage of 0.1% of the above precipitate weight, and treat for 1 h;

[0048] (8) Maintain the pH of the above enzymatic hydrolysate at 7.0~7.5, maintain the temperature at 55℃, and add flavor protease at a dosage of 0.1% of the above precipitate weight, and treat for 1 h;

[0049] (9) After centrifuging the final enzymatic hydrolysate at 4000 rpm for 10-15 min, take the supernatant and desalinate it using a nanofiltration membrane with a molecular weight cutoff of 300 Da. The nanofiltration parameters are: operating pressure 0.5-1.0 MPa and temperature 20-30℃. After nanofiltration, concentrate the volume to 1 / 2 and obtain the sesame peptide powder of the example by spray drying.

[0050] Comparative Example 1:

[0051] It consists of the traditional "alkali extraction and acid precipitation" steps, without enzymatic hydrolysis before alkali extraction, as detailed below:

[0052] (1) Sieving: Take 1 kg of sesame meal and sieve it through a 50-mesh sieve;

[0053] (2) Alkali extraction: Add 25 kg of purified water, add sodium hydroxide to adjust the pH to 12.5, and treat at 70℃ for 2 h;

[0054] (3) Precipitation by adding acid: Add hydrochloric acid to adjust the pH to 3.5 and treat for 1 hour;

[0055] (4) Centrifuge at 5000 rpm for 10 min to collect the precipitate, add 10 times the volume of purified water, and add alkaline protease at a dosage of 0.5% of the precipitate weight. Treat at 55℃ for 2 h.

[0056] (5) Add neutral protease to the above system at a dosage of 0.5% of the precipitate weight and treat for 4 h;

[0057] (6) After centrifugation at 4000 rpm for 10-15 min, the supernatant was collected and desalted using a nanofiltration membrane with a molecular weight cutoff of 300 Da. The nanofiltration parameters were: operating pressure 0.5-1 MPa and temperature 20-30℃. After nanofiltration, the volume was concentrated to 1 / 2 and spray-dried to obtain the sesame peptide powder of Comparative Example 1.

[0058] Comparative Example 2:

[0059] The difference between this comparative example and the examples is that in the initial enzymatic hydrolysis, only pectinase and cellulase were used (the amounts of pectinase and cellulase were the same as in the examples) to remove lipase and high-temperature amylase. All other operations were the same as in the examples, ultimately yielding the sesame peptide powder of Comparative Example 2.

[0060] Comparative Example 3:

[0061] The difference between this comparative example and the example is that the flavor protease in step 8 of the example was replaced with trypsin (the amount used was 0.1% of the precipitate collected in step (5)). All other operations were the same as in the example, and the sesame peptide powder of Comparative Example 3 was finally obtained.

[0062] Experimental Example 1: Determination of Total Protein Content of Sesame Peptides

[0063] The total protein content in the sesame peptides obtained in the examples and comparative examples was determined using the following methods:

[0064] The analysis was performed using a fully automated Kjeldahl nitrogen analyzer (HGK-55). Mixed indicator solution A (2 parts methyl red ethanol solution and 1 part methylene blue ethanol solution) or mixed indicator solution B (1 part methyl red ethanol solution and 5 parts bromocresol green ethanol solution) was prepared in advance. For analysis, a certain amount of sesame peptide sample obtained from the examples and comparative examples (accurate to 0.0001 g) was accurately weighed, and 0.4 g copper sulfate, 6 g potassium sulfate, and 20 mL sulfuric acid were added to the digestion furnace for digestion. After the digestion furnace temperature reached 420°C, digestion continued for at least 1 hour. At this point, the liquid in the digestion tube became green and transparent. The sample was then analyzed using a fully automated Kjeldahl nitrogen analyzer (before use, the analytical parameters were optimized according to different instruments, and sodium hydroxide (400 g / L) solution, sulfuric acid standard solution (0.05 mol / L), and boric acid solution containing mixed indicator A or B were added).

[0065] The content of each amino acid in the sample is calculated using the following formula:

[0066]

[0067] X: Protein content in the sample, in grams per 100 grams (g / 100 g);

[0068] V1: The volume of sulfuric acid standard titrant consumed by the test solution, in milliliters (mL);

[0069] V2: The volume of sulfuric acid standard titrant consumed by the reagent blank, in milliliters (mL);

[0070] c: Concentration of the sulfuric acid standard titration solution, in moles per liter (mol / L);

[0071] 0.0140: The mass of nitrogen equivalent to 1.0 mL of sulfuric acid standard titration solution, expressed in grams per millimole (g / mmol).

[0072] m: Mass of the sample, in grams or milliliters (g);

[0073] V3: The volume of digestive fluid taken, in milliliters (mL);

[0074] V4: The final volume of the digestion solution, in milliliters (mL);

[0075] F: Protein conversion factor, 5.30 for sesame peptide;

[0076] 100: Conversion factor from g / g to g / 100g.

[0077] Table 1. Total protein content in sesame peptides obtained in the examples and comparative examples.

[0078] method Example Comparative Example 1 Comparative Example 2 Comparative Example 3 Total protein content (%) 76.2 65.7 53.5 62.5

[0079] Experimental Example 2: Determination of Arginine Content in Sesame Peptides

[0080] The arginine content in the sesame peptides obtained in the examples and comparative examples was determined using the following methods:

[0081] Accurately weigh a certain amount of sesame peptide samples obtained from the examples and comparative examples (accurate to 0.0001 g), ensuring that the protein content in each sample is within the range of 10 mg to 20 mg. Depending on the protein content of the sample, add 10–15 mL of 6 mol / L hydrochloric acid solution to the hydrolysis tube, followed by 3–4 drops of phenol. Place the hydrolysis tube in a refrigerant and freeze at -21°C for 3–5 minutes. Connect it to the vacuum pump's suction pipe and evacuate (approximately 0 Pa). Then, fill with nitrogen. Repeat the evacuation and nitrogen filling process three times. Finally, seal or tighten the cap while the tube is filled with nitrogen.

[0082] Place the sealed hydrolysis tube in an electric heating oven at 110±1℃ for 22 h of hydrolysis, then remove and cool to room temperature. Open the hydrolysis tube and filter the hydrolysate through rapid quantitative filter paper into a 50 mL volumetric flask. Rinse the hydrolysis tube several times with a small amount of purified water, transferring the washings to the same 50 mL volumetric flask. Finally, dilute to the mark with water and shake to mix. Accurately pipette 1.0 mL of the filtrate into a 15 mL or 25 mL test tube and dry under reduced pressure using a tube concentrator or parallel evaporator at 40℃~50℃. After drying, dissolve the residue in 1~2 mL of water, dry under reduced pressure again, and finally evaporate to dryness. Dissolve the residue in 1~2 mL of pH 2.2 sodium citrate buffer solution in the dried test tube, shake to mix, and then pipette the solution through a 0.22 μm filter membrane to transfer it to the instrument's sample vial for instrument analysis.

[0083] The mixed amino acid standard working solution was injected into the automatic amino acid analyzer (Mannmerborough A388, Germany). Referring to the amino acid analyzer calibration procedures and instrument manual, the instrument operating procedures, parameters, and elution buffer solution reagent ratios were adjusted appropriately to confirm the instrument operating conditions. The mixed amino acid standard working solution and the sample assay solution were injected into the amino acid analyzer at equal volumes. The concentration of amino acids in the sample assay solution was calculated using the peak area method via the external standard method.

[0084] The amino acid content in the sample solution is calculated using the following formula:

[0085]

[0086] c i The content of amino acid i in the sample solution is measured in nanomoles per milliliter (nmol / mL).

[0087] Ai : Peak area of ​​amino acid i in the sample solution;

[0088] A s Peak area of ​​amino acids in amino acid standard working solution;

[0089] c s The content of amino acids S in the amino acid standard working solution is expressed in nanomoles per milliliter (nmol / mL).

[0090] The content of each amino acid in the sample is calculated using the following formula:

[0091]

[0092] X i The content of amino acid i in the sample, expressed in grams per 100 grams (g / 100 g);

[0093] c i The content of amino acid i in the sample solution, expressed in nanomoles per milliliter (nmol / mL);

[0094] F: Dilution factor;

[0095] V: The volume of the sample hydrolysate transferred and brought to a final volume, in milliliters (mL);

[0096] M: Molar mass of amino acid i, in grams per mole (g / mol);

[0097] m: Sample weight, in grams (g);

[0098] 10 9 The coefficient for converting sample content from nanograms (ng) to grams (g);

[0099] 100: Conversion factor.

[0100] Table 2 shows the arginine content in sesame peptides obtained from the examples and comparative examples.

[0101] method Example Comparative Example 1 Comparative Example 2 Comparative Example 3 Arginine content (%) 9.1 6.4 1.7 7.8

[0102] The results of total protein content determination in sesame peptides obtained in the examples and comparative examples are shown in Table 1, while the results of arginine content determination are shown in Table 2. It can be seen that the total protein content obtained in the examples accounts for 76.2% of the total weight of the sesame peptides (sesame peptides of the present invention), which is higher than that of the other comparative examples. The arginine content in the sesame peptides is 9.1% of the total protein content, also higher than that of the comparative examples. This indicates that the sesame peptides obtained by multi-step enzymatic hydrolysis of sesame meal in the present invention have higher total protein and arginine contents. Specifically, compared with the traditional "alkali dissolution and acid precipitation" (Comparative Example 1), the addition of an enzymatic hydrolysis system can significantly increase the total protein and arginine contents. Compared with Comparative Example 2, lipase and high-temperature amylase can further remove impurities, increase protein content, and avoid arginine flow cytometry. Compared with trypsin, flavor protease has a better effect in the extraction and purification of sesame peptides, thereby increasing the total protein and arginine contents.

[0103] Experimental Example 3: Effects of Sesame Peptide on Enhanced Sexual Function Indicators in TM3 Cell Model

[0104] This experimental example demonstrates the changes in the concentrations of testosterone, dihydrotestosterone, and cyclic guanosine monophosphate (cGMP) in the supernatant of a TM3 cell model after treatment with the sesame peptide of this invention. The experimental procedure is as follows:

[0105] Culture of TM3 cells:

[0106] TM3 cells were cultured in DMEM-F12 medium containing 10% horse serum, 2.5% fetal bovine serum, and 1% penicillin-streptomycin antibiotics. The cell culture incubator conditions were set to 37°C and 5% CO2. Once the cells reached a density of 80%-90%, the next step was passage and seeding. First, expired cell culture medium was discarded from the cell culture flasks, and the cells were washed three times with a suitable amount of preheated (37°C) PBS solution. After washing, an appropriate amount of trypsin was added for digestion. When some cells began to detach, a suitable amount of serum-containing medium was added, and then cells adhering to the bottom and walls of the flask were blown off with a pipette. The mixture was then collected, and the cell suspension was diluted appropriately. Cells were counted under a microscope using a cell counting chamber, and the appropriate number of cells were seeded according to different experimental requirements.

[0107] Measurement of testosterone, dihydrotestosterone, and cyclic guanosine monophosphate (cGMP) secretion levels:

[0108] TM3 cells were administered at a rate of 1×10 5The cells were seeded at a concentration of 1 / mL into 12-well plates and cultured at 37℃ and 5% CO2 saturated humidity for 24 h, after which the supernatant was discarded. Different experimental treatments were performed according to the following experimental groups: (1) Control group: only basal culture medium was added; (2) Positive control group: culture medium with a final mass concentration of sildenafil of 0.5 mg / mL; (3) Sample group: culture medium containing sesame peptide of the present invention at mass concentrations of 1 mg / mL and 2 mg / mL were added respectively; each concentration was set with 3 replicates and cultured for 48 h; the cell culture supernatant was collected, and then centrifuged at 1500 rpm for 5 min and the supernatant was taken. The testosterone ELISA kit (Nanjing Jiancheng, H090-1-2), dihydrotestosterone kit (Nanjing Jiancheng, H293-1-2) and cyclic guanosine cGMP assay kit (Baizhi Bio, EU19-96) were used to detect the secretion levels of testosterone, dihydrotestosterone and cGMP in TM3 cells.

[0109] Experimental results are as follows Figure 1 , Figure 2 as well as Figure 3 As shown, where, as Figure 1 As shown, the sesame peptide of the present invention can effectively increase the testosterone content in the TM3 cell model, and when the concentration of sesame peptide reaches 2 mg / mL, the sesame peptide can increase the testosterone level secreted by TM3 cells by more than 90% of that of sildenafil. Figure 2 As shown, the sesame peptide of the present invention can increase the content of dihydrotestosterone in the TM3 cell model to a certain extent, and the effect is enhanced with the increase of sesame peptide concentration. Figure 3 As shown, the sesame peptide of the present invention can increase the secretion level of the signaling molecule cyclic guanosine monophosphate (cGMP). This enhancement effect is positively correlated with the dosage used. When the concentration of sesame peptide reaches 2 mg / mL, the sesame peptide can increase the testosterone index of TM3 secretion by more than 85% of that of sildenafil.

[0110] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A sesame peptide, characterized in that, The preparation method of the sesame peptide includes the following steps: (a) Using pressed sesame meal as raw material, pre-process the sesame meal; (ii) Use the first enzyme group to remove impurities from the pretreated product; (iii) Protein extraction treatment of the enzymatic hydrolysate after enzymatic hydrolysis by the first enzyme group; (iv) Enzymatic hydrolysis of sesame protein in the product after protein extraction using the second enzyme group; The first enzyme group consists of pectinase, cellulase, lipase and high-temperature amylase; the second enzyme group consists of alkaline protease, neutral protease and flavor protease.

2. The sesame peptide as described in claim 1, characterized in that, In step (ii), the pretreated product is first enzymatically hydrolyzed by combining pectinase, cellulase and lipase, and then the hydrolysate obtained from the first enzymatic hydrolysis is hydrolyzed by the high-temperature amylase.

3. The sesame peptide as described in claim 1 or 2, characterized in that, The mass ratio of pectinase, cellulase, lipase and high-temperature amylase is 1:2:1:

1.

4. The sesame peptide as described in claim 1, characterized in that, In step (iii), alkaline protease, neutral protease, and flavor protease sequentially hydrolyze sesame protein.

5. The sesame peptide as described in claim 1 or 4, characterized in that, The mass ratio of alkaline protease, neutral protease, and flavor protease is 2:1:

1.

6. The sesame peptide as described in claim 1, characterized in that, The preprocessing in step (a) includes the following operations: (a) Sieve the sesame meal; (b) Soak the sieved sesame meal in water; (c) Acid wash the precipitate obtained from the soaking.

7. The sesame peptide as described in claim 1, characterized in that, Step (iii) involves using alkaline extraction and acid precipitation techniques to extract protein.

8. The sesame peptide as described in claim 7, characterized in that, In the alkaline extraction and acid precipitation technique, the pH of the alkaline extraction is 10.0, while the pH of the acid precipitation is 3.

7.

9. The sesame peptide as described in claim 1, characterized in that, The sesame peptide is prepared by the following steps: (1) Sieving: Take sesame meal, pass it through a 50-mesh sieve, and set aside; (2) Water soaking: Add purified water to the sieved sesame meal, treat at 90℃ for 1 hour, centrifuge at 4000 rpm for 10-15 minutes, and collect the precipitate; (3) Enzymatic hydrolysis: Add purified water to the collected precipitate, and add three enzymes according to the following dosage: 1% pectinase, 2% cellulase, and 1% lipase by weight of the precipitate. Maintain the temperature at 50°C and the pH at 4.5-5.5 for 1 hour. Then, raise the temperature of the enzymatic hydrolysate system to 80°C, adjust the pH to 7.0, and add high-temperature amylase according to the following dosage: 1% by weight of the precipitate. Treat for 1 hour. (4) Alkali extraction: Centrifuge at 5000 rpm for 10 min to collect the precipitate, add 3 L of purified water to the precipitate, adjust the pH to 10, and treat for 1 h; (5) Precipitation with acid: Centrifuge again at 5000 rpm for 10 min, collect the supernatant, adjust the pH of the supernatant to 3.7, treat for 1 h, and centrifuge again to collect the precipitate; (6) Add purified water to the precipitate collected in step (5) and adjust the pH value to 8.0~8.

5. Add alkaline protease at a dosage of 0.2% of the weight of the precipitate and treat at 55°C for 1 hour. (7) Adjust the pH of the above enzymatic hydrolysate to 7.0~7.5, maintain 55℃, and add neutral protease at a dosage of 0.1% of the weight of the precipitate collected in step (5), and treat for 1h; (8) Maintain the pH of the above enzymatic hydrolysate at 7.0~7.5, maintain the temperature at 55℃, and add flavor protease at a dosage of 0.1% of the weight of the precipitate collected in step (5), and treat for 1 hour; (9) After centrifuging the final enzymatic hydrolysate at 4000 rpm for 10-15 min, take the supernatant and desalt it using a nanofiltration membrane with a molecular weight cutoff of 300 Da. Concentrate the total volume to half of the original volume. The nanofiltration parameters are: operating pressure 0.5-1.0 MPa and temperature 20-30℃. Obtain the desired sesame peptide powder by spray drying.

10. An application of the sesame peptide as described in any one of claims 1 to 9, characterized in that, The application of the sesame peptide in the preparation of food, health products, or drugs that improve sexual dysfunction.

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