A high-yield erythritol strain of yarrowia lipolytica and its application

By knocking out the RAS2, EYD1, and A000092 genes in *Yamylostella lipolytica* and adding walnut shell biochar during fermentation, the problems of low yield and cell morphology transformation in *Yamylostella lipolytica* fermentation under high osmotic stress were solved, achieving efficient and stable erythritol production.

CN122234962APending Publication Date: 2026-06-19ZHEJIANG UNIV OF SCI & TECH
View PDF 1 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG UNIV OF SCI & TECH
Filing Date
2026-02-02
Publication Date
2026-06-19

AI Technical Summary

Technical Problem

In existing technologies, when Yeast Extractoria lipolytica ferments erythritol under high osmotic stress, the cell metabolic activity is inhibited, leading to slow fermentation, low yield, and increased cost. Furthermore, the pseudohyphae morphology is not conducive to cell metabolism and erythritol synthesis. How to effectively inhibit the transformation of cells to pseudohyphae morphology during fermentation and maintain the genetic stability of the strain remains a key issue.

Method used

A high-yield erythritol-producing strain of *Yarrowia lipolytica* was constructed by knocking out the RAS2, EYD1, and A000092 genes. Walnut shell biochar was added during the fermentation process to improve fermentation efficiency.

Benefits of technology

It significantly improved the fermentation yield and productivity of erythritol, shortened the metabolic engineering modification time, reduced production costs, and maintained the genetic stability of the strain.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN122234962A_ABST
    Figure CN122234962A_ABST
Patent Text Reader

Abstract

This invention discloses a high-yield erythritol-producing *Yarrowia lipolytica* strain and its applications. Using *Yarrowia lipolytica* CA20 as the starting strain, this invention constructs uracil via homologous recombination. ura3 Gene-deficient Yeast Extract CA20△ ura3 Using this strain as a base, the factors that cause the transformation of *Yarrowia lipolytica* from yeast morphology (oval) to hyphal morphology were successively knocked out. RAS2 Genes and key genes in the erythritol degradation pathway EYD1 and key genes related to erythritol metabolism. A000092 The engineered strain CAE1 was ultimately obtained. Under conditions of added walnut shell biochar, CAE1 achieved a maximum erythritol fermentation yield of 236.35 g / L, with a yield of 0.72 g / g and a production rate of 2.86 g / L / h. This method constructs a high-yielding strain that shortens the time required for metabolic engineering and significantly reduces the number of target sites during metabolic modification, providing a new technical route for the targeted breeding of high-yielding erythritol strains. The obtained high-yielding strain maintains stable erythritol production efficiency, and production costs are significantly reduced.
Need to check novelty before this filing date? Find Prior Art