Bacillus alvei CHWJ-P3 for dissolving phosphorus and potassium in acidic soil and application thereof
By screening and identifying Bacillus vesicularis CHWJ-P3, the problem of simultaneous activation of phosphorus and potassium nutrients in acidic soil was solved, achieving efficient dissolution and stable improvement of phosphorus and potassium in acidic soil, and providing an excellent resource for multifunctional microbial agents.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
- Filing Date
- 2026-05-22
- Publication Date
- 2026-06-23
Smart Images
Figure CN122256209A_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of microbial technology, and specifically relates to a Bacillus vesicularis CHWJ-P3 for dissolving phosphorus and potassium in acidic soil and its applications. Background Technology
[0002] Soil acidification is a global environmental challenge and one of the major obstacles limiting crop production. Its formation is closely related to excessive application of nitrogen fertilizer in agriculture, deposition of acidic gases from industry, and natural processes. Soil acidification not only directly inhibits plant root development but also leads to the fixation of essential nutrients such as phosphorus and potassium in the soil, forming forms that are difficult for plants to absorb and utilize. This creates a paradoxical situation where the soil nutrient pool is large but the available nutrients are severely lacking, forcing agricultural production to rely on large amounts of chemical fertilizers, which increases costs and exacerbates environmental risks.
[0003] To activate phosphorus and potassium nutrients fixed in the soil and reduce reliance on chemical fertilizers, the application of microbial fertilizers and inoculants has attracted much attention. Current technologies mainly utilize microorganisms with specific functions. For example, phosphate-solubilizing microorganisms (such as some strains of *Pseudomonas*) can convert insoluble phosphates in the soil into soluble forms by secreting organic acids and protons; while potassium-solubilizing microorganisms (such as *Bacillus cantoniensis*) can decompose minerals such as potassium feldspar and release potassium ions through acid production and complexation. These strains have shown potential in improving the availability of single elements such as phosphorus or potassium.
[0004] However, in actual agricultural production, crop growth requires a balanced supply of various nutrients such as phosphorus and potassium. The main shortcomings of current technologies are that most commercially available microbial agents have a single function or are simply mixtures of different strains. Single-function phosphate-solubilizing or potassium-solubilizing bacteria cannot simultaneously meet the crop's needs for multiple nutrients; furthermore, different strains in compound microbial agents may compete or antagonize each other, affecting their colonization and functional stability. Therefore, isolating a strain that simultaneously possesses highly efficient and stable phosphate-solubilizing and potassium-solubilizing abilities is of great significance for simplifying microbial agent production, ensuring synergistic effects, and achieving simultaneous biological activation of phosphorus and potassium nutrients in acidic soils. It is also an important direction for developing new, highly efficient microbial fertilizers. Summary of the Invention
[0005] To address the problems in the prior art, this invention provides a Bacillus sporogenes CHWJ-P3 strain for dissolving phosphorus and potassium in acidic soils and its applications. This strain can adapt to acidic environments and simultaneously has the function of efficiently dissolving inorganic phosphorus and decomposing potassium, providing an excellent strain resource for developing microbial agents for improving acidic soils and activating nutrients.
[0006] To achieve the above objectives, the technical solution adopted by the present invention is as follows:
[0007] A strain of *Paenibacillus alvei* CHWJ-P3, used for dissolving phosphorus and potassium in acidic soils, was deposited at the China Center for Type Culture Collection (CCTCC) on December 8, 2025, with accession number CCTCC NO: M 20252813.
[0008] Furthermore, the 16S rRNA sequence of the Bacillus vesicularis CHWJ-P3 is shown in SEQ ID NO: 1.
[0009] Furthermore, the *Bacillus vesicularis* CHWJ-P3 possesses functions of phosphate solubilization, potassium solubilization, and urease production.
[0010] Furthermore, the *Bacillus vesicularis* CHWJ-P3 exhibits acid resistance and can be cultured for 72 hours in acidic LB liquid medium at pH 4.0, showing an OD... 600 The value is not less than 1.1.
[0011] Specifically, the *Bacillus vesicularis* CHWJ-P3 strain was screened from soil from a plantation in Guangxi. On LB agar, it appears as an opaque, milky-white or pale yellow colony, round or nearly round with irregularly wavy edges, exhibiting a diffusive growth tendency. The surface displays fine, mucus-like textures and is smooth, moist, and slightly convex. It grows well in commonly used inorganic or organic culture media, with an optimal culture temperature between 25-35℃ and an optimal pH between 5-9.
[0012] An application of the aforementioned Bacillus vesicularis CHWJ-P3, wherein the Bacillus vesicularis CHWJ-P3 is used to prepare a microbial agent for improving acidic soil.
[0013] Furthermore, the *Bacillus vesicularis* CHWJ-P3 regulates soil acidification; specifically, the *Bacillus vesicularis* CHWJ-P3 is used for the biological improvement of phosphorus and potassium in acidic soils, that is, the *Bacillus vesicularis* CHWJ-P3 can dissolve and release insoluble phosphorus and potassium in acidic soils by releasing organic acids.
[0014] Furthermore, the *Bacillus vesicularis* CHWJ-P3 regulates the pH of acidic soils through its urease-producing ability.
[0015] An application of the aforementioned Bacillus vesicularis CHWJ-P3 involves applying a microbial agent containing the aforementioned Bacillus vesicularis CHWJ-P3 to acidic soil.
[0016] Furthermore, the application method is spraying or drip irrigation.
[0017] To ensure the normal growth and function of *Bacillus vesicularis* CHWJ-P3 in acidic soil, this invention investigated the acid tolerance of *Bacillus vesicularis* CHWJ-P3, including the following steps:
[0018] S1, strain enrichment
[0019] Add 10g of soil to 90mL of sterile water to make 10 -1 The soil dilution was prepared at a concentration of [concentration missing], and shaken at 180 rpm and 36°C for 2 hours. Then, it was continuously diluted to a concentration of 10x using a 10x dilution method. -8 To determine the concentration, 100 μL of different dilutions were spread onto LB solid medium and incubated at 36℃ for 72 h. Single colonies of different morphologies were then streaked for purification to obtain the native strains from acidic soil. The LB solid medium consisted of: 10 g / L peptone, 5 g / L yeast extract, 10 g / L sodium chloride, 20 g / L agar, and pH 7.0.
[0020] S2, assay of strain's urease production capacity
[0021] S21. Screening of strains capable of producing urease:
[0022] Single colonies of the purified strain were streaked onto a urease selection plate and incubated at 36°C for 24 hours. The color change of the culture medium (pale yellow → purplish red) was used to preliminarily determine whether the strain had the ability to produce urease; the deeper the red, the stronger the urease production ability.
[0023] Quantitative determination of urease production capacity of strain S22:
[0024] The strains with urease-producing ability obtained above were enriched in 100 mL of LB liquid medium. Then, 2 mL of the expanded culture was added to 100 mL of LBU liquid medium and cultured in a shaker at 36°C. After 24 hours of culture, the urease-producing ability of the strains was determined using the conductivity method, revealing a urease-producing capacity of 0.25–0.38 mmol·L⁻¹. -1 ·min -1 This demonstrates that the strain has a significant ability to produce urease.
[0025] The composition of LB liquid medium is: 10 g / L peptone, 5 g / L yeast extract, 10 g / L sodium chloride, pH=7.0.
[0026] The liquid urease screening medium (LBU) consists of: 20 g / L urea, 10 g / L peptone, 5 g / L yeast extract, and 10 g / L sodium chloride, pH 7.0. In addition, the solid urease screening medium also contains 20 g / L agar. (Note: The above media are sterilized after dissolving, and after cooling to room temperature, the urea solution is filtered through a sterile filter before being added to the media.)
[0027] S3, Phosphate-solubilizing ability of strains
[0028] S31. Screening of strains with phosphate-solubilizing ability:
[0029] After culturing the selected strains in LB medium for 24 hours, the bacterial culture was inoculated onto NBRIP solid medium containing sparingly soluble phosphorus using the spot inoculation method. The culture was then incubated at 36°C for 4-7 days. Strains with phosphorus-solubilizing ability were screened based on the formation and size of the phosphorus-solubilizing zone.
[0030] S32. Quantitative determination of strain phosphate solubility:
[0031] The obtained phosphorus-solubilizing strains were enriched in 100 mL of LB liquid medium. Then, 2 mL of the expanded culture was added to 100 mL of NBRIP liquid medium and cultured in a shaker at 36°C. After 72 hours of culture, the phosphorus content in the medium was measured, revealing a range of 40.23-112.62 mg / L, demonstrating the strain's significant phosphorus-solubilizing ability.
[0032] The NBRIP liquid medium consists of: glucose 10 g / L, Ca3(PO4)2 5 g / L, MgCl2·6H2O 5 g / L, MgSO4·7H2O 0.25 g / L, KCl 0.2 g / L, and (NH4)2SO4 0.1 g / L. In addition, the NBRIP solid medium contains 20 g / L agar.
[0033] S4. Determination of potassium solubilization ability of strains
[0034] S41. Screening of strains with potassium-solubilizing ability
[0035] After culturing the selected strains in LB medium for 24 hours, the bacterial solution was inoculated onto potassium-solubilizing solid medium using the spot inoculation method and cultured in an incubator at 36°C for 7 days. Strains with potassium-solubilizing ability were screened based on the formation and size of the potassium-solubilizing zone.
[0036] S42. Quantitative determination of potassium solubilization capacity of strain:
[0037] The obtained potassium-solubilizing strain was enriched in 100 mL of LB liquid medium. Then, 2 mL of the expanded culture was added to 100 mL of potassium-solubilizing liquid medium and cultured in a shaker at 36°C. After 72 hours of culture, the potassium content in the medium was measured, revealing a range of 1.25-72.81 mg / L, demonstrating the strain's significant potassium-solubilizing ability.
[0038] The composition of the potassium-solubilizing liquid medium is as follows: glucose 5 g / L, (NH4)2SO4 0.5 g / L, yeast extract 0.5 g / L, MgSO4•7H2O 0.3 g / L, NaH2PO4 2 g / L, FeSO4•7H2O 0.03 g / L, MnSO4•H2O 0.03 g / L, and potassium feldspar 2 g / L. In addition, the potassium-solubilizing solid medium also contains 20 g / L of agar.
[0039] S5, acid resistance test of strain
[0040] Based on the functional assay results of the above strains, a milky white, opaque strain with urease production, phosphorus solubilization, and potassium solubilization capabilities was screened and named CHWJ-P3. This strain was then enriched in 100 mL of LB medium in a shaker at 36°C.
[0041] After 24 hours of cultivation, 2 mL of the expanded bacterial culture was added to 10 mL of LB liquid medium with a pH of 4-7 and cultured in a shaker at 36°C. After 72 hours of cultivation, the bacterial concentration in the medium was measured, and the bacterial concentration was found to be within the OD range. 600 =1.139-3.610, proving that the strain can survive in an acidic contaminated environment and therefore has acid resistance.
[0042] S6 strain's pH regulation performance in acidic soil was tested.
[0043] Take 2 mL of the expanded bacterial culture and put it into 1 g of acidic soil with pH 5. Incubate in a constant temperature incubator at 36℃. After 10-30 days of incubation, measure the pH of the soil and find that the soil pH has risen to 5.2.
[0044] S7. Taxonomic identification of strains
[0045] The urease-producing, phosphate-solubilizing, and potassium-solubilizing strains obtained through screening were identified using molecular biology methods. Their 16S rRNA sequences were determined and compared with the GenBank nucleic acid database. The sequence alignment confirmed that they were Paenibacillus alvei CHWJ-P3. The taxonomic name of Paenibacillus alvei CHWJ-P3 was determined and deposited at the China Center for Type Culture Collection on December 8, 2025, with accession number CCTCC NO: M 20252813.
[0046] Compared with the prior art, the present invention has the following beneficial effects:
[0047] The Bacillus sporogenes CHWJ-P3 of this invention has a significant ability to dissolve phosphorus and potassium in the environment, high acid resistance and efficient pH adjustment ability. It can be used for the biological improvement of phosphorus and potassium dissolution in acidic soils, and has the characteristics of high remediation efficiency, stable improvement effect and no secondary pollution, and has potential application prospects. Attached Figure Description
[0048] Figure 1 These are screening images of Bacillus vesicularis CHWJ-P3 in this invention;
[0049] Figure 2 These are scanning electron microscope (SEM) images of Bacillus vesicularis CHWJ-P3 in this invention, wherein: (a) is a scanning electron microscope image at a magnification of 5000x (5.00 KX), and (b) is a scanning electron microscope image at a magnification of 20000x (20.00 KX);
[0050] Figure 3 This is the developmental tree of Bacillus vesicularis CHWJ-P3 in this invention.
[0051] Preservation Instructions
[0052] The taxonomic name of the Bacillus alvei CHWJ-P3 is Paenibacillus alvei CHWJ-P3. It was deposited on December 8, 2025 at the China Center for Type Culture Collection (CCTCC), Wuhan University, Wuhan, Hubei Province, China, with accession number CCTCC NO: M 20252813. Detailed Implementation
[0053] The present invention will be further described below with reference to embodiments.
[0054] The relevant information of the commercial reagents involved in the examples is shown in Table 1 below:
[0055] Table 1. Main experimental drugs and their corresponding manufacturers
[0056]
[0057] Example 1: Separation of CHWJ-P3
[0058] The bacterial strain CHWJ-P3 was screened from soil from a plantation in Guangxi. The specific screening steps are as follows:
[0059] S1, strain enrichment
[0060] Add 10g of soil to 90mL of sterile water to make 10 -1 The soil dilution was prepared at a concentration of [concentration missing], and shaken at 180 rpm and 36°C for 2 hours. Then, it was continuously diluted to a concentration of 10x using a 10x dilution method. -8 To determine the concentration, 100 μL of different dilutions were spread onto LB agar plates and incubated at 36°C for 72 h. Single colonies of different morphologies were then streaked for purification to obtain the native strain from acidic soil. The described *Bacillus apiaceus* CHWJ-P3 is as follows: Figure 1 As shown, it appears as an opaque milky white or pale yellow colony on LB solid medium. The colonies are round or irregular in shape, raised, with a glossy and moist surface, irregularly wavy edges, and fine, mucous-like textures around the edges. The colonies spread in a dendritic pattern along the streaks, covering the entire LB plate within 48 hours. The scanning electron microscope image of the *Bacillus vesicularis* CHWJ-P3 is shown below. Figure 2 As shown, (a) is a scanning electron microscope image at a magnification of 5000x (5.00 KX), and (b) is a scanning electron microscope image at a magnification of 20000x (20.00 KX). It is long rod-shaped with many wrinkles on the surface.
[0061] The composition of LB solid medium is: 10 g / L peptone, 5 g / L yeast extract, 10 g / L sodium chloride, 20 g / L agar, pH=7.0; specifically, each 1 L of LB solid medium includes 10 g peptone, 5 g yeast extract, 10 g sodium chloride, 20 g agar, and the pH of LB solid medium is 7.0.
[0062] Example 2: Determination of urease, phosphate solubilizing, and potassium solubilizing activities of CHWJ-P3
[0063] S1, Determination of urease production capacity of strain S1
[0064] S11. Screening of strains with urease-producing ability:
[0065] Single colonies of the purified strain were streaked onto a urease selection plate and incubated at 36°C for 24 hours. The color change of the culture medium (pale yellow → purplish red) was used to preliminarily determine whether the strain had the ability to produce urease; the deeper the red, the stronger the urease production ability.
[0066] Quantitative determination of urease production capacity of strain S12:
[0067] The strains with urease-producing ability obtained above were enriched in 100 mL of LB liquid medium. Then, 2 mL of the expanded culture was added to 100 mL of LBU liquid medium and cultured in a shaker at 36°C. After 24 hours of culture, the urease-producing ability of the strains was determined using the conductivity method, revealing an enzyme production capacity of 0.25–0.38 mmol·L⁻¹. -1 ·min -1 This demonstrates that the strain has a significant ability to produce urease.
[0068] The composition of LB liquid medium is: 10 g / L peptone, 5 g / L yeast extract, 10 g / L sodium chloride, pH=7.0; specifically, each 1L of LB liquid medium includes 10 g peptone, 5 g yeast extract, 10 g sodium chloride, and the pH of LB liquid medium is 7.0.
[0069] The composition of urease screening liquid medium (LBU) is: 20 g / L urea, 10 g / L peptone, 5 g / L yeast extract, 10 g / L sodium chloride, pH=7.0. In addition, the urease screening solid medium also contains 20 g / L agar. Specifically, each 1 L of urease screening solid medium (LBU) includes: 20 g urea, 10 g peptone, 5 g yeast extract, 10 g sodium chloride, 20 g agar, 1 L deionized water, and a pH of 7.0. (Note: The above medium is sterilized after dissolving, and after cooling to room temperature, the urea solution is filtered through a sterile filter before being added to the medium.)
[0070] S2, Phosphate-solubilizing ability of strains
[0071] S21. Screening of strains with phosphate-solubilizing capabilities:
[0072] After culturing the selected strains in LB medium for 24 hours, the bacterial culture was inoculated onto NBRIP solid medium containing sparingly soluble phosphorus using the spot inoculation method. The culture was then incubated at 36°C for 4-7 days. Strains with phosphorus-solubilizing ability were screened based on the formation and size of the phosphorus-solubilizing zone.
[0073] S22. Quantitative determination of the phosphorus-solubilizing ability of strain S22:
[0074] The obtained phosphorus-solubilizing strains were enriched in 100 mL of LB liquid medium. Then, 2 mL of the expanded culture was added to 100 mL of NBRIP liquid medium and cultured in a shaker at 36°C. After 72 hours of culture, the phosphorus content in the medium was measured, revealing a range of 40.23-112.62 mg / L, demonstrating the strain's significant phosphorus-solubilizing ability.
[0075] The NBRIP liquid medium consists of: 10 g / L glucose, 5 g / L Ca3(PO4)2, 5 g / L MgCl2·6H2O, 0.25 g / L MgSO4·7H2O, 0.2 g / L KCl, and 0.1 g / L (NH4)2SO4. In addition, the NBRIP solid medium contains 20 g / L agar; specifically, each 1 L of NBRIP solid medium includes 10 g glucose, 5 g Ca3(PO4)2, 5 g MgCl2·6H2O, 0.25 g MgSO4·7H2O, 0.2 g KCl, 0.1 g (NH4)2SO4, 20 g agar, and 1 L deionized water.
[0076] S3, Determination of potassium solubilization ability of strains
[0077] S31. Screening of strains with potassium-solubilizing ability
[0078] After culturing the selected strains in LB medium for 24 hours, the bacterial solution was inoculated onto potassium-solubilizing solid medium using the spot inoculation method and cultured in an incubator at 36°C for 7 days. Strains with potassium-solubilizing ability were screened based on the formation and size of the potassium-solubilizing zone.
[0079] S32. Quantitative determination of potassium solubilization capacity of strain:
[0080] The obtained potassium-solubilizing strain was enriched in 100 mL of LB liquid medium. Then, 2 mL of the expanded culture was added to 100 mL of potassium-solubilizing liquid medium and cultured in a shaker at 36°C. After 72 hours of culture, the potassium content in the medium was measured, revealing a range of 1.25-72.81 mg / L, demonstrating the strain's significant potassium-solubilizing ability.
[0081] The composition of the potassium-solidifying solid medium is as follows: glucose 5 g / L, (NH4)2SO4 0.5 g / L, yeast extract 0.5 g / L, MgSO4•7H2O 0.3 g / L, NaH2PO4 2 g / L, FeSO4•7H2O 0.03 g / L, MnSO4•H2O 0.03 g / L, potassium feldspar 2 g / L, and agar 20 g / L. Specifically, each 1 L of potassium-solidifying solid medium includes 5 g glucose, 0.5 g (NH4)2SO4, 0.5 g yeast extract, 0.3 g MgSO4•7H2O, 2 g NaH2PO4, 0.03 g FeSO4•7H2O, 0.03 g MnSO4•H2O, 2 g potassium feldspar, 20 g agar, and 1 L deionized water.
[0082] The composition of the potassium-solubilizing liquid culture medium is as follows: glucose 5 g / L, (NH4)2SO4 0.5 g / L, yeast extract 0.5 g / L, MgSO4•7H2O 0.3 g / L, NaH2PO4 2 g / L, FeSO4•7H2O 0.03 g / L, MnSO4•H2O 0.03 g / L, and potassium feldspar 2 g / L. Specifically, each 1 L of potassium-solubilizing liquid culture medium includes 5 g glucose, 0.5 g (NH4)2SO4, 0.5 g yeast extract, 0.3 g MgSO4•7H2O, 2 g NaH2PO4, 0.03 g FeSO4•7H2O, 0.03 g MnSO4•H2O, 2 g potassium feldspar, and 1 L deionized water.
[0083] Example 3: Taxonomic identification of strains
[0084] The urease-producing, phosphate-solubilizing, and potassium-solubilizing strains obtained through screening were identified using molecular biology methods. Their 16S rRNA sequences were determined and compared with the GenBank nucleic acid database. Sequence alignment confirmed them as *Paenibacillus alvei* CHWJ-P3. This *Paenibacillus alvei* CHWJ-P3 was taxonomically named and deposited at the China Center for Type Culture Collection (CCTCC) on December 8, 2025, with accession number CCTCC NO: M 20252813. The 16S rRNA sequence of *Paenibacillus alvei* CHWJ-P3 is shown in SEQ ID NO: 1.
[0085] SEQ ID NO: 1 is as follows:
[0086]
[0087] like Figure 3 As shown, the phylogenetic tree results of Paenibacillus alvei CHWJ-P3 show that CHWJ-P3 has the highest homology with Paenibacillus alvei strain NBRC 3343 (GenBank accession number NR_113577.1), with a sequence matching degree of 98.85%. Therefore, CHWJ-P3 can be classified as Paenibacillus alvei.
[0088] Example 4: Determination of acid tolerance of Bacillus vesicularis CHWJ-P3
[0089] The isolated *Bacillus vesicularis* strain CHWJ-P3 was inoculated into 100 mL of LB liquid medium with pH values of 4, 5, 6, and 7, and cultured in a shaker at 36°C. After 72 hours of culture, the bacterial concentration in the liquid medium was measured, and it was found that the strain could survive and grow in a strongly acidic environment at pH 4.0. The acid tolerance of CHWJ-P3 is shown in Table 2, and the activity of CHWJ-P3 at different pH values is shown in Table 3.
[0090] Table 2 Activity of CHWJ-P3 at different pH values
[0091]
[0092] Table 3. Acidity regulation ability of CHWJ-P3 at different pH values.
[0093]
[0094] Example 5: Application method and effects of microbial agents
[0095] S1. Application Period
[0096] The CHWJ-P3 strain was inoculated into LB liquid medium and cultured at 36°C with shaking for 24 h to obtain the seed culture. The culture was then transferred to a fermenter at a 5% (w / w) inoculation rate for further culture for 48 h, resulting in a viable cell count of 2.0 × 10⁻⁶ cells / mL. 9 CFU / mL. CHWJ-P3 microbial agent can be sprayed as a base fertilizer before sowing or transplanting each crop. When used for paddy field improvement, it should be applied 3-5 days before plowing, harrowing, or soaking the field. When used for dryland farmland improvement, it should be applied 5-10 days or more before sowing or transplanting seedlings and promptly plowed into the field.
[0097] S2. Application amount is shown in Table 4.
[0098] Table 4 Recommended application rate
[0099]
[0100] The higher the degree of soil acidification, the greater the amount of microbial agent should be applied, with a maximum annual application rate not exceeding 10,000 L / acre. Microbial agents can be applied continuously, with an interval of at least one month and no more than one year between applications. For clay or sandy soils, application should be done in batches, with an recommended interval of 1-2 hours depending on the penetration of the microbial agent.
[0101] S3. Application Method
[0102] S31. After determining the amount of microbial agent to be applied based on the needs and depth of the target soil improvement or remediation, the microbial agent can be applied to the soil surface by spraying or drip irrigation (it is recommended to choose a sunny and windless day). Then, use agricultural tillage machinery to break up and till the soil. It is recommended that the tillage depth be < 50cm, and 15cm~30cm is appropriate.
[0103] S32 can be used in conjunction with other soil conditioners (such as phosphate fertilizer, nitrogen fertilizer, biochar, phosphorus-containing minerals, etc.).
[0104] S33. For soils that are severely acidified and prone to compaction, a second tillage can be carried out within 15 days of the first tillage, and bacterial solution can be added according to the actual test results.
[0105] S34. The condition of the soil and field surface after tillage should meet the requirements of the rotary tiller operation quality standard (NY / T 499-2013).
[0106] S4. Precautions
[0107] Before applying S41 and CHWJ-P3 microbial agents, in addition to meeting the requirements of the target soil improvement, it is also necessary to take into account the adaptability of the target soil to the crops being grown, so as to avoid improper use affecting the normal growth and development and yield of the crops.
[0108] S42. When applying microbial agents, if the soil is too acidic, a soil pH conditioner should be used to adjust the pH before application. However, environmentally friendly, non-toxic, and heavy metal-free reagents or materials must be selected to avoid microbial death or damage and pollution to other soil properties.
[0109] S5. Application Effect
[0110] CHWJ-P3 inoculant was applied to acidic soil with a pH of 5. Ryegrass and rice were planted in the acidic soil after applying 10% (v / w) of CHWJ-P3 inoculant. Soil physicochemical properties were measured after a 30-day pot culture experiment.
[0111] S51, Effects of acidic soil improvement
[0112] CHWJ-P3 microbial agent can adjust the pH of acidic soil to 6.3 within 3-5 months. In terms of pH, the adjusted soil is suitable for planting most crops.
[0113] S52, Promoting effects
[0114] After treatment with CHWJ-P3, the available phosphorus and available potassium contents in the soil increased by 86.3-128.6% and 65.2-84.8%, respectively. The germination rate of rice and ryegrass increased by 18.2-26.3%, and the biomass increased by 86.3-119.7%.
[0115] The Bacillus sporogenes CHWJ-P3 strain in this invention can adapt to acidic environments and has outstanding functions in efficiently dissolving inorganic phosphorus and decomposing potassium, providing an excellent strain resource for developing multifunctional microbial agents for acidic soil improvement and nutrient activation.
[0116] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements can be made without departing from the principle of the present invention, and these improvements should also be considered within the scope of protection of the present invention.
Claims
1. A Bacillus sporogenes CHWJ-P3 for dissolving phosphorus and potassium in acidic soils, characterized in that, The *Paenibacillus alvei* CHWJ-P3 strain, classified as *Paenibacillus alvei* CHWJ-P3, was deposited at the China Center for Type Culture Collection (CCTCC) on December 8, 2025, with accession number CCTCC NO: M 20252813. This *Paenibacillus alvei* CHWJ-P3 strain is acid-resistant and can be cultured for 72 hours in acidic LB broth at pH 4.
0. OD 600 The value is not less than 1.
1.
2. The *Bacillus vesicularis* CHWJ-P3 for dissolving phosphorus and potassium in acidic soil according to claim 1, characterized in that, The 16S rRNA sequence of the Bacillus vesicularis CHWJ-P3 is shown in SEQ ID NO:
1.
3. The *Bacillus apiaceus* CHWJ-P3 for dissolving phosphorus and potassium in acidic soil according to claim 1, characterized in that, The aforementioned Bacillus sporogenes CHWJ-P3 has the functions of phosphate solubilization and potassium solubilization.
4. The *Bacillus vesicularis* CHWJ-P3 for dissolving phosphorus and potassium in acidic soil according to claim 1, characterized in that, The aforementioned Bacillus sporogenes CHWJ-P3 has the function of producing urease.
5. The use of Bacillus vesicularis CHWJ-P3 according to any one of claims 1-4 in the preparation of microbial agents for improving acidic soil.
6. The application according to claim 5, characterized in that, The described Bacillus vesicularis CHWJ-P3 dissolves and releases insoluble phosphorus and potassium in acidic soil.
7. The application according to claim 5, characterized in that, The aforementioned Bacillus sporogenes CHWJ-P3 regulates the pH of acidic soils through its ability to produce urease.
8. The application according to claim 5, characterized in that, The microbial agent containing the aforementioned Bacillus vesicularis CHWJ-P3 was applied to the acidic soil.
9. The application according to claim 8, characterized in that, The application method is spraying or drip irrigation.