Wenyang Tongzhu Tongzi Decoction Compound Moxa Stick and Preparation Method and Application in Improving Reproductive System Function

Abstract

This invention discloses a compound moxa stick for warming yang and unblocking obstruction, its preparation method, and its application in improving reproductive system function. It belongs to the field of external preparations of traditional Chinese medicine. Addressing the problems of limited improvement effects of pure moxa sticks, lack of systematic design in compound moxa stick formulations, unclear synergistic effects between components, large fluctuations in conditioning effects, and difficulty in balancing conditioning effects and safety, this invention uses specific weight proportions of Artemisia argyi, Cyperus rotundus, Alpinia galanga, Cinnamomum cassia, Zingiber officinale, Illicium verum, Cinnamomum cassia twig, Spatholobus suberectus, Caesalpinia sappan, Smilax glabra, Euonymus fortunei, Citrus aurantium, Amomum villosum, Citrus reticulata peel, and Atractylodes lancea to prepare a compound moxa stick. The components synergistically enhance each other, and this product is used to prepare a moxibustion intervention product for improving the reproductive function of mammals. It can stably improve female ovarian reserve function and male asthenospermia, simultaneously regulate reproductive-related hormone levels, repair pathological damage to reproductive organs, and improve the quality of reproductive cells.

Description

Technical Field

[0001] This invention belongs to the field of external preparations of traditional Chinese medicine, specifically relating to a compound moxa stick for warming yang and unblocking obstruction, its preparation method, and its application in improving reproductive system function. Background Technology

[0002] Decreased ovarian reserve and asthenospermia are common conditions that cause a decline in reproductive capacity in mammals and are also one of the important causes of infertility in humans. At present, the commonly used intervention methods in clinical practice are mainly hormone drugs. Although these methods can improve relevant physiological indicators to a certain extent, they have problems such as a high incidence of adverse reactions and poor patient compliance with long-term medication, making it difficult to meet the actual needs of long-term and gentle conditioning.

[0003] Moxibustion, a traditional external treatment method in Traditional Chinese Medicine, has long been used in the treatment of reproductive system-related diseases. The smoke produced by burning mugwort is one of the core pathways through which it exerts its therapeutic effect. Currently, most moxa sticks used for reproductive function regulation are pure moxa sticks made from single mugwort leaves. Their effects on improving ovarian reserve function and sperm motility are limited, and it is difficult to achieve simultaneous and stable improvement in reproductive hormone levels, reproductive organ tissue morphology, and reproductive cell quality. Some compound moxa sticks used for reproductive regulation lack a systematic design for improving reproductive function in their ingredient formulation. It is difficult for the various ingredient components to form a clear synergistic effect, resulting in significant batch-to-batch and individual-to-individual fluctuations in the therapeutic effect. At the same time, it is difficult to balance the therapeutic effect and the safety of use in the development of such compound moxa sticks. How to improve reproductive function while avoiding adverse effects on normal tissues is also a major technical challenge in the development of related products. Summary of the Invention

[0004] One object of the present invention is to solve at least the above-mentioned problems and to provide at least the advantages that will be described later.

[0005] Another objective of this invention is to provide a compound moxa stick containing warming yang and unblocking obstruction, which can stably improve the ovarian reserve function of female mammals and asthenospermia of male mammals through the synergistic effect of multiple raw material components. It can simultaneously regulate the level of reproductive hormones, repair pathological damage to reproductive organ tissues, and improve the quality of reproductive cells. Moreover, while playing a role in improving reproductive function, it will not have any adverse effects on normal tissues of the body, and has good safety and stable efficacy.

[0006] To achieve these objectives and other advantages of the present invention, a compound moxa stick with warming and unblocking properties is provided, characterized in that it is composed of the following raw material components in parts by weight: 15-20 parts of mugwort leaves, 0.5-1.0 parts of cyperus rhizome, 0.5-1.0 parts of galangal, 0.5-1.0 parts of cinnamon, 0.1-0.5 parts of dried ginger, 0.1-0.5 parts of star anise, 0.1-0.5 parts of cassia twig, 0.5-1.0 parts of chicken blood vine, 0.1-0.5 parts of sappanwood, 0.1-0.5 parts of *Millettia speciosa*, 0.1-0.5 parts of *Euonymus fortunei*, 0.5-1.0 parts of citron, 0.1-0.5 parts of amomum villosum, 0.5-1.0 parts of dried tangerine peel, and 0.1-0.5 parts of atractylodes lancea.

[0007] This invention uses mugwort as its core ingredient, combined with Cyperus rotundus, Alpinia galanga, cinnamon, dried ginger, star anise, cassia twig, Spatholobus suberectus, Sappanwood, Ficus pumila, Euonymus fortunei, Citrus medica, Amomum villosum, Citrus reticulata peel, and Atractylodes lancea in a specific ratio to form a compound moxa stick. The components work synergistically, utilizing the smoke released during combustion as a pathway to achieve multi-target synergistic effects. This stabilizes and regulates the body's reproductive hormone levels, repairs pathological damage to reproductive organs such as the ovaries and uterus, improves follicle development quality and oocyte maturation rate, and simultaneously improves sperm motility and acrosome structure integrity. It has a clear effect on improving ovarian reserve and asthenospermia, and the intervention does not adversely affect normal tissues such as the lungs. The effect is stable and has good safety.

[0008] A method for preparing a compound moxa stick containing a warming and unblocking formula includes the following steps: Weigh out 15-20 parts by weight of Artemisia argyi, 0.5-1.0 parts of Cyperus rotundus, 0.5-1.0 parts of Alpinia galanga, 0.5-1.0 parts of Cinnamomum cassia, 0.1-0.5 parts of Zingiber officinale, 0.1-0.5 parts of Star anise, 0.1-0.5 parts of Cinnamomum cassia twig, 0.5-1.0 parts of Spatholobus suberectus, 0.1-0.5 parts of Caesalpinia sappan, 0.1-0.5 parts of Moghania macrophylla, 0.1-0.5 parts of Euonymus fortunei, 0.5-1.0 parts of Citrus medica, 0.1-0.5 parts of Amomum villosum, 0.5-1.0 parts of Citrus reticulata peel, and 0.1-0.5 parts of Atractylodes lancea. All raw materials except mugwort leaves are placed in a grinder and ground. After grinding, the powder is passed through a 150-mesh sieve to obtain fine powder of traditional Chinese medicine. Artemisia leaves are processed into Artemisia floss with a ratio of 5-10:1; Weigh the corresponding materials according to the mass ratio of moxa floss to fine Chinese medicine powder of 1:0.5-5, place them in a mixing container and stir continuously to make the fine Chinese medicine powder evenly dispersed in the moxa floss, so as to obtain the mixed moxa floss and medicine. The mixture of medicinal and moxa wool was weighed quantitatively according to the fixed filling amount of a single moxa stick; Mulberry bark paper is used as the paper for rolling. The quantitatively measured mixture of medicinal moxa wool is placed evenly in the moxa stick rolling machine, and the rolling machine is started to complete the rolling, resulting in a moxa stick blank with a diameter of 1.8-2.2 cm and a length of 19-21 cm. The joints and end faces of the moxa stick blank are sealed with special sealing adhesive for moxa sticks; The sealed moxa stick blank is placed in a cool and ventilated place to air dry naturally, resulting in the finished compound moxa stick.

[0009] In the existing preparation process of compound moxa sticks, uneven mixing of medicinal powder and moxa wool often occurs, resulting in poor consistency in the size and compactness of the moxa sticks. Volatile medicinal components in the raw materials are also easily lost during processing, ultimately leading to unstable release of medicinal components when the finished moxa sticks are burned, significant fluctuations in the therapeutic effect on reproductive function, and obvious quality differences between different batches of products. This invention first weighs mugwort leaves and other complementary medicinal materials in a fixed ratio. All medicinal materials except mugwort leaves are pulverized and sieved to obtain uniform and fine Chinese herbal powder. Mugwort leaves are processed separately into moxa wool with a specification of 5-10:1. Then, moxa wool and Chinese herbal powder are weighed in a fixed mass ratio and placed in a mixing container for thorough stirring and mixing to ensure that the Chinese herbal powder is evenly dispersed in the moxa wool to obtain a mixed medicinal and mugwort wool. The mixed medicinal and mugwort wool is quantitatively weighed according to the fixed filling amount of a single moxa stick. Using mulberry bark paper as the rolling paper, it is rolled in a standardized manner to obtain moxa stick blanks with uniform size and specifications. The joints and end faces of the blanks are then sealed, and finally, they are placed in a cool and ventilated place to air dry naturally to obtain the finished product. The entire preparation process is completed at room temperature, which can retain the volatile medicinal components in the raw materials to the greatest extent and avoid damage to the effective substances caused by high-temperature processing. The standardized material handling, mixing and rolling process can ensure that the medicinal powder is evenly dispersed in the moxa wool, and the specifications and compactness of the finished moxa sticks remain highly consistent. This makes the release of medicinal components during the burning of the moxa sticks stable and controllable, greatly reducing the quality difference between different batches of products, ensuring the stability and repeatability of the product's reproductive function conditioning effect, and at the same time, the smooth connection between each process can adapt to the needs of large-scale production and processing.

[0010] Preferably, the mugwort leaves are processed into mugwort floss with a ratio of 5-10:1, including the following steps: Take aged mugwort leaves that have been stored for 24-36 months and place them in a sealed environment at a temperature of 20-25 ℃ and a relative humidity of 40%-50% for 12-24 hours to allow the moisture content of the mugwort leaves to stabilize at 8%-12%. After the mugwort leaves have been rehydrated, they are fed into a hammer mill and coarsely crushed using a screen with a mesh diameter of 8-10 mm. The hard stems and petioles of the mugwort leaves are separated and removed to obtain coarse mugwort floss. The coarse Artemisia flakes are fed into a multi-stage air classifier unit and passed through air classification channels with progressively decreasing wind speeds of 3-5 levels. The wind speed in the first air classification channel is controlled at 6-8 m / s, and the wind speed in each subsequent air classification channel is reduced by 0.8-1.2 m / s compared to the previous level. After air classification and separation, the flakes with fiber lengths in the range of 3-8 mm are collected to obtain the initial selected Artemisia flakes. The initial selected mugwort floss is placed in an environment with a temperature of 15-20 ℃ and sieved using a 40-60 mesh vibrating screen to remove the mugwort leaf dust and short floss fragments that are sieved. The material on the sieve is collected to obtain the finished mugwort floss with a floss extraction ratio of 5-10:1.

[0011] As the core raw material for compound moxa sticks, existing processing methods often only broadly limit the final moxa wool extraction ratio without precise control over the processing process, fiber morphology, and impurity content. The resulting moxa wool often has uneven fiber lengths and high levels of dust and stem-like impurities. This not only makes it difficult for the fine powder of traditional Chinese medicine to adhere stably to the fiber gaps, but also easily leads to powder stratification and shedding. This results in uneven content of medicinal components in different parts of the moxa stick, causing drastic fluctuations in the release of effective components during combustion. The residual impurities can also lead to incomplete combustion of the moxa wool, producing additional irritating smoke and affecting the safety and consistency of the moxa stick's use. This invention selects aged Artemisia argyi leaves stored for 24-36 months. First, the leaves are placed in a sealed environment at 20-25°C and 40%-50% relative humidity for 12-24 hours to rehydrate, stabilizing the moisture content at 8%-12% to prevent excessive fiber breakage during subsequent pulverization. The rehydrated leaves are then fed into a hammer mill and coarsely pulverized using a sieve with an 8-10 mm aperture. The hard stems and petioles are separated and removed to obtain coarse Artemisia argyi fibers. These fibers are then fed into a multi-stage air classifier, passing through 3-5 stages of progressively decreasing airflow. The first stage maintains an airflow speed of 6-8 m / s, with each subsequent stage decreasing by 0.8-1.2 m / s. After air separation, fibers with a length of 3-8 mm are collected. The collected fibers are then placed at a temperature of 15-20°C. In an environment of ℃, a 40-60 mesh vibrating screen is used for sieving to remove the mugwort dust and short fibers that are sieved. The material remaining on the screen is collected to obtain finished mugwort floss with a fiber extraction ratio of 5-10:1. This processing method can significantly reduce the amount of impurities in the mugwort floss while precisely controlling the fiber extraction ratio. The specific length fibers obtained by sieving can form a stable three-dimensional porous structure, providing sufficient attachment sites for the fine powder of traditional Chinese medicine. This effectively avoids the stratification and shedding of the powder during subsequent mixing, ensuring that the content of medicinal components in each part of the moxa stick is uniform. At the same time, the resulting mugwort floss burns fully and stably, which can reduce the generation of irritating smoke and make the release of effective components during the burning of the moxa stick stable and controllable. Ultimately, this ensures the consistency of the conditioning effect and the safety of use of the compound moxa stick.

[0012] Preferably, after collecting the initially selected moxa wool and before vibrating sieving, the following steps are also included: The selected moxa wool is evenly spread on a breathable mesh belt and placed in a closed environment with a temperature of 15-20 ℃ and a relative humidity of 55%-65%. It is continuously sprayed with deionized water with an atomization particle size of 10-20 μm for humidification for 30-60 minutes to stabilize the overall moisture content of the moxa wool at 10%-14%. The humidified and initially selected mugwort floss is fed into a roller loosening machine. The rollers with a gap of 2-4 mm and arc-shaped protrusions on the surface are used to continuously roll and loosen the floss 2-3 times to break up the fiber clumps inside the mugwort floss and obtain mugwort floss in a monofilament dispersed state. The monofilament dispersed mugwort floss is fed into a high-voltage electrostatic separator, and the separation electric field strength is controlled at 80-120kV / m to separate and remove lignocellulose, hard debris and non-fibrous impurities from the mugwort floss, thus obtaining purified mugwort floss. The purified mugwort floss is fed into a vibrating screen for subsequent sieving processing.

[0013] The initial moxa wool obtained through mechanical crushing and multi-stage air separation is prone to static electricity on the fiber surface due to mechanical friction, forming a large number of tangled clumps. The clumps easily trap dust, fragments, and lignified impurities, which are difficult to effectively remove during subsequent sieving. The residual impurities can lead to incomplete combustion of the moxa sticks and produce additional irritating smoke. At the same time, the clumped moxa wool cannot form a uniform and continuous pore structure. When it is mixed with fine Chinese medicine powder, the powder is difficult to embed evenly into the fiber gaps, which can easily cause uneven distribution of medicinal components in different parts of the moxa stick and large fluctuations in the release of effective components during combustion. This invention involves evenly spreading the collected pre-selected moxa floss onto a breathable mesh belt and placing it in a sealed environment with a temperature of 15-20 ℃ and a relative humidity of 55%-65%. Continuous spraying humidification is performed using deionized water with an atomized particle size of 10-20 μm, with the processing time controlled at 30-60 min, stabilizing the overall moisture content of the moxa floss at 10%-14%. The humidified pre-selected moxa floss is then fed into a roller-type loosening machine, where rollers with a gap of 2-4 mm and arc-shaped protrusions on their surfaces are used for 2-3 consecutive rolling presses to loosen the fiber clumps within the moxa floss, obtaining monofilament dispersed moxa floss. Subsequently, the monofilament dispersed moxa floss is fed into a high-voltage electrostatic separator, with the separation electric field strength controlled at 80-120 kV / m, separating and removing ligninized fibers, hard debris, and non-fibrous impurities from the moxa floss. The purified moxa floss is then fed into a vibrating screen for subsequent sieving.

[0014] Low-temperature atomization humidification can precisely control the moisture content of moxa wool, eliminate static electricity on the fiber surface, and create suitable conditions for the loosening of fiber clumps. Roller pressing can disperse the tangled fibers into uniform monofilaments without damaging the main fibers of the moxa wool, preventing the fibers from continuing to entangle and clump together. At the same time, by utilizing the difference in dielectric constant between the cellulose fibers of moxa wool and lignified impurities, non-effective fiber impurities can be accurately removed through high-voltage electrostatic separation, completing the purification process of moxa wool. This results in the final moxa wool forming a uniform and stable three-dimensional porous structure, which not only ensures that the subsequent sieving process can completely remove residual dust and debris from the moxa wool, but also significantly improves the uniformity of mixing moxa wool with fine Chinese medicine powder. This allows the resulting moxa sticks to burn more completely and stably, with uniform and controllable release of effective ingredients, while reducing the generation of irritating smoke and ensuring the safety and consistency of the therapeutic effect of the moxa sticks.

[0015] Preferably, after obtaining the purified Artemisia floss and before feeding it into a vibrating screen for sieving, the following steps are also included: The purified moxa wool is evenly spread on a breathable mesh belt, with the thickness of the spread material controlled at 5-10 mm. It is placed in a constant flow ventilation environment with a temperature of 18-22℃ and a relative humidity of 35%-45%, with the ventilation speed controlled at 1-2 m / s and the treatment time at 20-40 min, so that the overall moisture content of the moxa wool is stabilized at 8%-10%. The dehumidified mugwort floss is fed into a roller-type static eliminator with a polytetrafluoroethylene insulating layer on the surface. The roller speed is controlled at 15-25 r / min, the ion air pressure inside the roller is 0.1-0.2 MPa, and the treatment time is 10-20 min to eliminate the residual static electricity on the surface of the mugwort floss fibers. After static electricity is eliminated, the moxa wool is passed through a magnetic separation channel with a magnetic field strength of 0.3-0.5 T to remove metal debris mixed in with the moxa wool; The processed mugwort floss is fed into a vibrating screen for subsequent screening.

[0016] The purified moxa wool, after being treated with atomization humidification, roller pressing and loosening, and high-voltage electrostatic separation, has a generally high moisture content. Directly entering the subsequent screening process can easily lead to fiber adhesion and screen blockage, resulting in the inability to completely remove dust and short fibers from the moxa wool. The high moisture content can also cause the volatile medicinal components in the medicinal raw materials to absorb moisture and degrade during the subsequent mixing with fine Chinese medicine powder. At the same time, the mechanical treatment of roller pressing and loosening and high-voltage electrostatic separation can cause the moxa wool fibers to generate surface static electricity again, which can easily lead to fiber agglomeration, uneven mixing of medicinal powder, and inconsistent tightness of moxa stick rolling in the subsequent screening process. Multiple mechanical processing steps may also introduce trace metal debris into the moxa wool, posing a risk of releasing pollutants during combustion and damaging subsequent processing equipment. In this invention, the purified moxa floss is evenly spread on a breathable mesh belt, with the thickness of the spread material controlled at 5-10 mm. It is placed in a constant flow ventilation environment with a temperature of 18-22 ℃ and a relative humidity of 35%-45%, and the ventilation speed is controlled at 1-2 m / s. The treatment time is 20-40 min, so that the overall moisture content of the moxa floss is stabilized at 8%-10%. The dehumidified moxa floss is then sent into a roller-type electrostatic eliminator with a polytetrafluoroethylene insulating layer on the surface. The roller speed is controlled at 15-25 r / min, the ion air pressure inside the roller is 0.1-0.2 MPa, and the treatment time is 10-20 min to eliminate residual static electricity on the surface of the moxa floss fibers. Subsequently, the electrostatically eliminated moxa floss is passed through a magnetic separation channel with a magnetic field strength of 0.3-0.5 T to remove metal debris mixed in with the moxa floss. Finally, the processed moxa floss is sent to a vibrating screen for subsequent screening processing. The gentle dehumidification method of low-temperature constant-flow ventilation can precisely adjust the moisture content of moxa wool to the optimal range for subsequent processing without damaging the volatile effective components in the moxa wool. This eliminates the risks of fiber adhesion and screen blockage, and also avoids the moisture absorption and degradation of volatile components of medicinal powder in subsequent mixing stages. Within this optimal moisture content range, a roller-type ion wind electrostatic elimination treatment can thoroughly remove residual static electricity from the fiber surface without dead corners, preventing fiber and medicinal powder agglomeration in subsequent stages. This ensures that the moxa wool is fully sieved, the medicinal powder is evenly mixed, and the moxa sticks are rolled with consistent tightness. The magnetic separation treatment with a constant magnetic field can efficiently remove trace metal debris mixed in the moxa wool, eliminating safety hazards without damaging the main fibers of the moxa wool. Ultimately, this ensures the efficacy stability, batch-to-batch consistency, and safety of use of the finished moxa sticks.

[0017] Preferably, the corresponding materials are weighed according to a mass ratio of 1:0.5-5 of moxa floss and fine Chinese medicine powder, placed in a mixing container, and continuously stirred and turned to ensure that the fine Chinese medicine powder is evenly dispersed in the moxa floss, thus obtaining a mixed moxa floss and medicine mixture. The specific steps include: The interior of the mixing container should be grounded to prevent static electricity, and the temperature of the mixing operation environment should be controlled at 18-22 ℃, and the relative humidity at 40%-50%. Weigh out the finished moxa floss and fine Chinese medicine powder at a mass ratio of 1:0.5-5. Place all the weighed finished moxa floss into a mixing container, start the mixing container's stirring mechanism, and stir at a low speed of 5-10 r / min to make the moxa floss form a fluffy, suspended fiber bed. Divide the weighed Chinese herbal powder into 3-5 equal portions. Use anhydrous ethanol atomized gas with a particle size of 5-15 μm as the carrier gas. Evenly convey each portion of Chinese herbal powder to the loose fiber bed in the mixing container at a rate of 0.5-1.0 kg / min. After conveying each portion of Chinese herbal powder, maintain a speed of 5-10 r / min and continue to stir for 3-5 min before conveying the next portion of Chinese herbal powder. After all the fine powder of Chinese medicine has been transported, the mixing environment parameters are kept constant, and the mixture is continuously stirred at a speed of 8-12 r / min for 8-12 min to obtain a premixed velvet. The premixed floss is fed into a double-roll press with a roller gap of 5-8 mm and subjected to low-pressure rolling once or twice, so that the fine powder of traditional Chinese medicine is embedded in the three-dimensional porous structure of the mugwort floss fiber to obtain the mixed floss of medicine and mugwort.

[0018] In the preparation of compound moxa sticks, conventional stirring and mixing methods can easily damage the long fiber structure of moxa wool during stirring, causing the collapse of the three-dimensional porous structure inside the moxa wool. This makes it impossible to provide stable retention sites for the fine powder of traditional Chinese medicine. During subsequent processing and storage, the powder is prone to stratification and shedding. At the same time, the friction between the fibers, the powder, and the container wall can easily generate static electricity, causing the fine powder of traditional Chinese medicine to agglomerate and the container wall to absorb and lose powder. This results in a large deviation between the actual powder ratio loaded in the moxa wool and the set ratio. The distribution of each component of traditional Chinese medicine in the moxa wool is uneven, and the fine powder of traditional Chinese medicine with different densities is also prone to gravity stratification. Lighter components float while heavier components settle. Ultimately, this results in significant differences in the content of medicinal components in different parts of the finished moxa stick. The release of effective components fluctuates violently during combustion, and it is difficult to guarantee the batch-to-batch stability of the reproductive function conditioning effect and the consistency of the intervention of a single moxa stick throughout the entire process. To address this issue, the present invention first grounds and prevents static electricity inside the mixing container, controlling the temperature of the mixing environment to 18-22 ℃ and the relative humidity to 40%-50%. Then, finished moxa wool and fine Chinese medicine powder are weighed according to a fixed mass ratio. All the finished moxa wool is placed in the mixing container, and the stirring mechanism is started at a low speed of 5-10 r / min to form a loose, suspended fiber bed. The weighed fine Chinese medicine powder is then divided into 3-5 equal portions. Anhydrous ethanol atomized gas with a particle size of 5-15 μm is used as the carrier gas to uniformly convey each portion of fine Chinese medicine powder to the loose fiber bed in the mixing container at a rate of 0.5-1.0 kg / min. After each portion of fine Chinese medicine powder is conveyed, the original speed is maintained for 3-5 minutes of continuous stirring before conveying the next portion. After all the fine Chinese medicine powder has been conveyed, the original operating environment parameters are maintained, and stirring is continued at a speed of 8-12 r / min for 8-12 minutes. The premixed floss is obtained by mining. Finally, the premixed floss is fed into a double-roll press with a roller gap of 5-8 mm and subjected to low-pressure rolling 1-2 times to embed the fine powder of Chinese medicine into the three-dimensional pore structure of the mugwort floss fiber, thus obtaining the mixed floss of Chinese medicine and mugwort.A grounded, anti-static working environment and precise temperature and humidity control reduce static electricity generation during mixing. The loose, suspended fiber bed formed by low-speed tumbling fully opens the three-dimensional pores inside the moxa wool without damaging its long fiber structure, providing ample embedding sites for the fine powder of traditional Chinese medicine. Using anhydrous ethanol atomized gas as the carrier gas eliminates static electricity on the surface of the fibers and powder without dead angles, preventing powder agglomeration and container wall adsorption loss. It also evenly transports the fine powder of traditional Chinese medicine into the fiber bed, achieving uniform dispersion of the powder within the pores of the moxa wool. The batch feeding combined with staged tumbling effectively avoids gravity stratification of powders of different densities, ensuring uniform distribution of each component of traditional Chinese medicine in the moxa wool. The final low-pressure roller pressing process allows the powder to be stably embedded in the pores of the moxa wool fibers, preventing powder stratification and detachment in subsequent processes. This ensures that the ratio of moxa wool to powder meets the set requirements, resulting in stable release of medicinal components during the burning of the finished moxa stick. This achieves batch-to-batch consistency of the reproductive function conditioning effect of compound moxa sticks and stability of the intervention of a single moxa stick throughout the entire process.

[0019] The application of the Wenyang Tongzu Yanzi compound moxa stick in the preparation of moxibustion intervention products to improve the reproductive function of mammals, specifically improving the ovarian reserve function of female mammals or improving asthenospermia in male mammals.

[0020] Application of moxa wool in the preparation of moxibustion products that improve ovarian reserve function in female mammals.

[0021] The present invention has at least the following beneficial effects: First, the combination of multiple medicinal herbs with mugwort as the core of this invention can form a stable synergistic effect. It can not only effectively improve the decline of ovarian reserve function, restore the level of reproductive hormones to the normal range, repair pathological damage to the ovaries and uterus, and improve the quality of follicle development and oocyte maturation rate, but also improve the symptoms of asthenospermia, improve sperm motility and viability, and protect the integrity of sperm acrosome structure, thus achieving simultaneous improvement of reproductive function in both sexes. Compared with pure moxa sticks, the conditioning effect is more significant and the scope of action is more comprehensive.

[0022] Secondly, this invention effectively solves the problems of uneven mixing of medicinal powder and moxa wool, easy loss of effective ingredients, and large fluctuations in the release of medicinal effects during combustion in traditional compound moxa sticks by using a refined moxa wool preparation process, a standardized powder mixing process, and a quantitative rolling process. It not only preserves the volatile medicinal components in the raw materials to the greatest extent, but also ensures the batch consistency of the specifications, compactness, and medicinal component content of the finished moxa sticks. This makes the release of medicinal components during the burning of the moxa sticks stable and controllable, and greatly reduces the batch-to-batch and individual-to-individual fluctuations in the conditioning effect.

[0023] Third, this compound moxa stick uses natural Chinese medicinal materials as raw materials. Animal experiments have verified that while improving reproductive function, it will not cause pathological damage to normal tissues such as the lungs, and there is no obvious risk of irritation. Compared with conventional clinical hormone intervention programs, its mode of action is mild, without the adverse reactions of hormone drugs, and can meet the needs of long-term conditioning. At the same time, the preparation process is smooth and the parameters are controllable, which can be adapted to large-scale industrial production and has good prospects for industrial application.

[0024] Other advantages, objectives and features of the present invention will become apparent in part from the following description, and in part from those skilled in the art through study and practice of the invention. Attached Figure Description

[0025] Figure 1 HE staining pathological images of mouse ovarian tissue from each group; Figure 2 Microscopic images of mouse oocyte morphology in each group; Figure 3 HE staining pathological images of testicular tissue from male mice in each group; Figure 4 HE staining pathological images of lung tissue from female mice in each group; Figure 5 HE staining pathological images of lung tissue from male mice in each group. Detailed Implementation

[0026] The present invention will now be described in further detail with reference to the accompanying drawings, so that those skilled in the art can implement it based on the description.

[0027] Example 1 This embodiment provides a compound moxa stick containing the following ingredients by weight: 16.75 g of mugwort leaves, 0.9 g of cyperus rhizome, 0.6 g of galangal, 0.6 g of cinnamon, 0.3 g of dried ginger, 0.3 g of star anise, 0.3 g of cassia twig, 0.9 g of chicken blood vine, 0.3 g of sappanwood, 0.3 g of *Millettia speciosa*, 0.3 g of *Euonymus fortunei*, 0.6 g of citron, 0.3 g of amomum villosum, 0.6 g of dried tangerine peel, and 0.3 g of atractylodes lancea.

[0028] The preparation method of the above-mentioned warming and unblocking compound moxa stick includes the following steps: Weigh out 16.75 g of Artemisia argyi, 0.9 g of Cyperus rotundus, 0.6 g of Alpinia galanga, 0.6 g of Cinnamomum cassia, 0.3 g of Zingiber officinale, 0.3 g of Star anise, 0.3 g of Cinnamomum cassia twig, 0.9 g of Spatholobus suberectus, 0.3 g of Sappanwood, 0.3 g of Fructus Massa, 0.3 g of Stellaria media, 0.6 g of Citrus medica, 0.3 g of Amomum villosum, 0.6 g of Citrus reticulata peel, and 0.3 g of Atractylodes lancea. All raw materials except mugwort leaves are placed in a pulverizer and pulverized. After pulverization, the powder is passed through a 150-mesh sieve to obtain fine powder of traditional Chinese medicine. Mugwort leaves are processed into 8:1 grade mugwort floss using conventional methods (take aged mugwort leaves that have been stored for 24 months, coarsely pulverize them to remove hard stems and coarse residues, and then process them into 8:1 grade mugwort floss using conventional floss extraction technology, i.e., 1 kg of finished mugwort floss is extracted from every 8 kg of mugwort leaves). Weigh the corresponding materials according to the mass ratio of moxa floss to fine Chinese medicine powder of 1:1, place them in a mixing container and stir continuously to make the fine Chinese medicine powder evenly dispersed in the moxa floss, so as to obtain the mixed moxa floss of medicine. The mixture of medicinal and moxa wool was weighed quantitatively according to the fixed filling amount of a single moxa stick; Mulberry bark paper was used as the paper for rolling. The quantitatively measured mixture of medicinal moxa wool was placed evenly in the moxa stick rolling machine, and the rolling machine was started to complete the rolling, resulting in a moxa stick blank with a diameter of 2.0 cm and a length of 20 cm. The joints and end faces of the moxa stick blank are sealed with special sealing adhesive for moxa sticks; The sealed moxa stick blank is placed in a cool and ventilated place to air dry naturally, resulting in the finished compound moxa stick.

[0029] Effect test 1.1 Grouping of experimental animals and preparation of experimental models SPF-grade ICR mice were selected, with females aged 6-8 weeks and weighing 22±2g; and males aged 7-9 weeks and weighing 24±2g. They were purchased from a facility with a valid laboratory animal production license, and the experimental protocol was reviewed and approved by the facility's laboratory animal ethics committee. Mice were housed in an SPF-grade animal room with a constant temperature of 22±2℃, relative humidity of 50±5%, and a 12-hour light-dark cycle. They had free access to food and water and were acclimatized for one week before the experiment. The experiment consisted of four groups, with 12 female and 12 male mice in each group (n=12). ① Control group: Both female and male mice were injected intraperitoneally with an equal volume of sterile saline, without any moxibustion intervention; ② Model group (cyclophosphamide group): Female mice were injected intraperitoneally with 75 mg / kg cyclophosphamide to establish a model of decreased ovarian reserve, while male mice were injected intraperitoneally with 60 mg / kg cyclophosphamide once a day for 5 consecutive days to establish a model of asthenospermia. No moxibustion intervention was performed. ③ Single moxa intervention group: The modeling method is the same as that of the model group. Starting from the 7th day after the modeling is completed, pure moxa sticks are used for moxibustion intervention. ④ Compound intervention group: The modeling method is the same as that of the model group. Starting from the 7th day after the modeling is completed, the compound moxa sticks prepared in this embodiment are given moxibustion intervention.

[0030] The moxibustion intervention method used a self-made sealed moxibustion box with internal dimensions of 60 cm (length) × 60 cm (width) × 180 cm (height), and an effective volume of 0.648 m³.3 The box is equipped with adjustable ventilation openings and a moxa smoke concentration monitoring port on its side walls, and a standardized moxa stick fixing device at the bottom. Moxa fumigation intervention was conducted daily at 18:00. Each time, one moxa stick of the same specification (8:1 pure moxa stick for the single moxa intervention group, and the compound moxa stick of this embodiment for the compound intervention group, each stick containing 18 g of filling material, 2.0 cm in diameter, and 20 cm in length) was fixed inside the device and lit. Mice were placed in the upper stainless steel cage of the moxa fumigation box and subjected to moxa fumigation for 30 minutes in a sealed environment. This was done once daily, 7 days a week, for 4 consecutive weeks. A portable laser dust meter was used to monitor the PM10 total particulate matter concentration inside the box in real time, recording the data every 5 minutes. The concentration was stabilized at 10±2 mg / m³ by adjusting the ventilation opening. 3 To ensure that the intervention dose is consistent across all groups.

[0031] 1.2 Effects of Artemisia argyi smoke on the general condition and body weight of female mice Observe the mice's mental state, fur condition, and activities such as eating and drinking daily; record the mice's weight and compare the weight changes of mice in each group.

[0032] 1.3 Effects of Artemisia argyi smoke on uterine and ovarian indices in female mice After modeling and moxibustion smoke intervention, mice were anesthetized with 1% sodium pentobarbital. Both uteruses and ovaries were harvested and weighed, and the uterine index and ovarian index were calculated. Uterine index (mg / g) = wet uterine weight / body weight; Ovarian index (mg / g) = wet weight of both ovaries / body weight.

[0033] 1.4 Effects of Artemisia argyi smoke on serum hormone levels in female mice After modeling, mice were anesthetized with 1% sodium pentobarbital, and blood samples were collected. After standing at room temperature for 60 min, the samples were centrifuged at 4 ℃ and 3500 r / min for 15 min, and the supernatant serum was collected. The serum AMH, FSH, LH, and E2 levels were measured according to the ELISA kit instructions.

[0034] 1.5 Effects of Artemisia argyi smoke on ovarian follicle development in female mice Mouse ovarian tissue fixed with paraformaldehyde was dehydrated, cleared, embedded in paraffin, and then sectioned in 4 μm thick paraffin. After dewaxing and hydration, the tissue was stained with hematoxylin and eosin (HE) and mounted with neutral resin. The morphology of the ovarian tissue was observed, and the number of follicles at each stage was counted. Preantral follicles and antral follicles were classified as developing follicles. The rate of developing follicles (%) was calculated as follows: (Number of developing follicles / Total number of follicles in mice) × 100%; the rate of atretic follicles (%) was calculated as follows: (Number of atretic follicles / Total number of follicles in mice) × 100%.

[0035] 1.6 Effects of Artemisia argyi smoke on oocyte maturation in female mice At 21 days after modeling and moxibustion intervention, mice in each group were injected with PMSG for 48 hours, followed by hCG for 14-16 hours to induce ovulation. Under a microscope, the dilated portion of the fallopian tube was punctured with a 1 ml needle to release the cumulus oophorus complex. Oocytes were then isolated using hyaluronidase, and the oocyte maturation rate was calculated for each group. Oocyte maturation rate (%) = number of oocytes in metaphase II (MIV) / total number of oocytes obtained × 100%.

[0036] 1.7 Effects of Artemisia argyi smoke on the histology of male mouse testes Mouse testicular tissue fixed with paraformaldehyde was dehydrated, cleared, impregnated with paraffin, and embedded. Paraffin sections with a thickness of 4 μm were then dewaxed, hydrated, stained with hematoxylin and eosin (HE), and mounted with neutral resin for observation of the testicular tissue.

[0037] 1.8 Effects of Artemisia argyi smoke on sperm-related parameters in mice Computer-assisted sperm assay (CASA) is used to detect sperm density, motility, viability, and various sperm motility parameters in mice. The main indicators detected include: sperm density, sperm motility, sperm viability, curve-line velocity (VCL), straight-line velocity (VSL), average path velocity (VAP), amplitude of lateral head displacement (ALH), linearity (LIN), wobble (WOB), and straightness (STR).

[0038] 1.9 Effects of Artemisia argyi smoke on lung tissue of female and male mice Lung tissues from female and male mice fixed with paraformaldehyde were dehydrated, cleared, impregnated with paraffin, and embedded. Paraffin sections with a thickness of 4 μm were dewaxed, hydrated, stained with hematoxylin and eosin (HE), and mounted with neutral resin to observe the morphology of the lung tissues.

[0039] 2. Experimental Results 2.1 Effects of Artemisia argyi smoke on the general condition and body weight of mice Mice in the blank control group showed good mental condition, with smooth and shiny fur, and normal and regular feeding and drinking. Mice in the cyclophosphamide group showed poor mental condition, with reduced activity, rough fur, and poor appetite in some cases. Mice treated with single-ingredient Artemisia (Artemisia leaf processed into moxa wool at a ratio of 8:1) and compound Artemisia (Moxa sticks prepared in Example 1) showed improved mental condition, with increased activity, restored fur luster, and normalized feeding and drinking. Weight recovered (Table 1).

[0040] Table 1 Comparison of mouse body weight among groups

[0041] 2.2 Effects of Artemisia argyi smoke on uterine and ovarian indices in mice After intervention with single-ingredient and compound-ingredient moxa, the skin regained its rosy color, and the ovarian and uterine indices of the compound-ingredient moxa group increased significantly (P<0.05) (Table 2).

[0042] Table 2 Comparison of ovarian index and uterine index among different groups of mice

[0043] Note: Compared with the cyclophosphamide group * P<0.05.

[0044] 2.3 Effects of Artemisia argyi smoke on serum hormone levels in female mice Compared with the control group, the model group mice showed significantly decreased serum AMH and E2 levels, while significantly increased FSH and LH levels (P < 0.05). Compared with the model group mice, the single-herb and compound-herb groups showed significantly increased AMH levels (P < 0.05), the compound-herb group showed significantly increased E2 levels (P < 0.05), the single-herb and compound-herb groups showed significantly decreased LH levels (P < 0.05), and the compound-herb group showed significantly decreased FSH levels (P < 0.05). The steady increase in AMH and E2 levels indicates enhanced follicle development capacity.

[0045] Table 3. Reproductive hormone levels in each group of mice

[0046] Note: Different letters indicate significant differences.

[0047] 2.4 Effects of Artemisia argyi smoke on ovarian follicle development in female mice Compared with the control group, the model group showed a significant decrease in the rate of developing follicles and a significant increase in the rate of atretic follicles (P < 0.05). Compared with the model group, the single-ingredient and compound-ingredient groups showed a significant increase in the rate of developing follicles and a significant decrease in the rate of atretic follicles (P < 0.05).

[0048] The effects of single-ingredient and compound-ingredient Artemisia argyi were very good, and the rate of follicle development had returned to normal levels, with no significant difference compared to the control group (P > 0.05). Figure 1 (and Table 4).

[0049] Table 4. Developmental status of follicles and atretic follicles in each group of mice.

[0050] Note: Compared with the blank group, ** P <0.01; compared with the cyclophosphamide group, △△ P <0.01.

[0051] 2.5 Effects of Artemisia argyi smoke on oocyte maturation in female mice In the control group, oocytes showed uniform, smooth, and unfragmented cytoplasmic granules, moderate perivitelline space, round or elliptical polar bodies, and smooth, clear, and uniform zona pellucida. However, in the cyclophosphamide model group, oocytes exhibited varying degrees of cytoplasmic fragmentation. After intervention with compound acetaminophen and single acetaminophen, the oocyte fragmentation rate decreased, the cytoplasm became uniform, the perivitelline space became moderate, and the polar bodies became round or elliptical. Figure 2 ).

[0052] Compared with the control group (86.90%), the oocyte maturation rate of mice in the cyclophosphamide model group was only 66.50%, which was significantly lower (P<0.05). Compared with the cyclophosphamide model group, the oocyte maturation rate was significantly restored to 70.50% after intervention with compound acetaminophen, and significantly restored to 86.69% after intervention with single acetaminophen (P<0.05) (Table 5).

[0053] Table 5. Maturation status of mouse oocytes in each group

[0054] Note: Different letters indicate significant differences. 2.6 Effects of Artemisia argyi smoke on testicular tissue of male mice The results are as follows Figure 3As shown in the diagram. In the control group mice, no obvious abnormalities were observed in the testicular tissue structure. The seminiferous tubules were round or oval, intact and full. The spermatogonia were round, regularly arranged, and closely attached to the basement membrane of the seminiferous tubules. The spermatogenic cells at all levels were arranged in an orderly manner with distinct layers, and the spermatocyte structure was clear, with a large number of sperm visible in the lumen. The Sertoli cells had normal morphology, and the interstitial cells were clustered. In the testicular tissue of the model group mice, the seminiferous tubules were atrophied, the spermatogenic cells at all levels were disordered and loosely connected, and both the layers and the number were reduced. The interstitial spaces were widened, and the number of spermatocytes and mature sperm in the lumen was reduced. The interstitial cells showed mild edema. After intervention with compound Artemisia argyi and single Artemisia argyi, the morphology and structure of the testicular tissue were improved to varying degrees. The seminiferous tubules were round or oval, and the structure was basically full. The number of spermatogenic cells at all levels increased, the layers were restored, and the number of spermatocytes and mature sperm was increased in the lumen.

[0055] 2.7 Effects of Artemisia argyi smoke on relevant indicators in male mice There was no difference in sperm density among the groups (P>0.05); the sperm motility and viability of the cyclophosphamide group were significantly reduced (P<0.05); after intervention with compound Ai and single Ai, the sperm motility and viability of the mice were significantly improved (P<0.05), and there was no significant difference compared with the control group (P>0.05).

[0056] In the cyclophosphamide group, the velocity of curvilinear movement (VCL), velocity of linear movement (VSL), average path velocity (VAP), amplitude of sperm head lateral swing (ALH), linearity (LIN), oscillation (WOB), and spurious movement (STR) were significantly reduced (P < 0.05). After intervention with compound acetaminophen and single acetaminophen, the VCL, VSL, VAP, ALH, LIN, WOB, and STR of mice were significantly improved (P < 0.05), and there was no significant difference compared with the control group (P > 0.05).

[0057] Table 6. Sperm density, motility, and viability in each group of mice.

[0058] Table 7. Sperm motility parameters of mice in each group

[0059] Note: Different letters indicate significant differences. 2.8 Effects of Artemisia argyi smoke on the lungs of female and male mice HE staining showed that the lung tissue structure was intact and clear in the female and male control groups, the combined intervention group, and the single-Ai intervention group, with visible bronchioles and terminal bronchioles. Cubic and squamous epithelial cells covered the bronchial lumen. Type I and type II alveolar cells were structurally normal, with a greater number of squamous type I alveolar cells and a smaller number of cuboidal type II alveolar cells. Alveolar ducts, alveolar sacs, and alveoli were visible in all groups. Some bronchioles showed incomplete openings, indicating their connection to the alveoli. The alveoli were vesicular, polyhedral, and without nodular enlargement. No obvious abnormalities were observed in the combined intervention group and the single-Ai intervention group. Figure 4 , Figure 5 .

[0060] Example 2 This embodiment provides a compound moxa stick with a warming and unblocking formula, which differs from Embodiment 1 in that the mugwort leaves are processed into moxa floss with an 8:1 ratio, including the following steps: Aged mugwort leaves stored for 24 months were placed in a sealed environment at 20 ℃ and 45% relative humidity for 18 hours to rehydrate, stabilizing the moisture content at 10%. The rehydrated mugwort leaves were then fed into a hammer mill and coarsely pulverized using a 9mm mesh screen to separate and remove the hard stems and petioles, yielding coarse mugwort floss. This coarse floss was then fed into a multi-stage air classifier, passing through four stages of progressively slower air classifiers. The speed in the first stage was controlled at 7 m / s, and each subsequent stage reduced the speed by 1.0 m / s. After air separation, floss with a fiber length within the range of 5mm was collected to obtain preliminary mugwort floss. This preliminary floss was then placed in an environment at 18 ℃ and sieved using a 50-mesh vibrating screen to remove the undersized dust and short floss fragments. The remaining material was collected to obtain finished mugwort floss with a floss extraction ratio of 8:1.

[0061] Effect test 1. Fiber length qualification rate test: The distribution of mugwort fiber length is quantitatively determined by microscopic image analysis technology. The proportion of fibers that meet the core qualification range of 3-8mm in this invention is counted, which is the fiber length qualification rate. The higher the ratio, the better the quality of the mugwort fiber meets the design requirements of the invention.

[0062] Testing steps: 1) Sampling: Five parallel samples were randomly selected from the finished moxa wool, each containing 10 mg, and equilibrated in a constant temperature and humidity environment for 24 hours to ensure stable moisture content.

[0063] 2) Slide preparation: Disperse each sample evenly on a glass slide, add a drop of neutral glycerol dispersant to prevent fiber overlap and entanglement, cover with a coverslip, place under an upright biological microscope, and take panoramic images with a 10× objective lens. Take no less than 10 non-overlapping fields of view for each sample.

[0064] 3) Measurement: The length of a single fiber is identified and measured using an image analysis system. Stem impurities with a diameter > 0.5 mm are removed, and the total number of fibers in each sample and the number of qualified fibers in the 3-8 mm range are counted.

[0065] 4) Parallel verification: The relative deviation of 5 parallel samples must be ≤5%; otherwise, resampling and testing are required.

[0066] Calculation method: Fiber length pass rate (%) = (Number of fibers in the pass range / Total number of fibers) × 100%; The final result is the arithmetic mean of 5 parallel samples.

[0067] Simultaneously, serum AMH, follicle rate, and oocyte maturation rate were measured in female mice, while sperm motility, VCL, and VSL were measured in male mice.

[0068] The compound moxa sticks prepared in this embodiment achieved a qualified rate of 89.62±2.48% for moxa fiber length (3-8 mm), which is significantly higher than the qualified rate of 68.42±3.26% for moxa sticks prepared by the conventional process in Example 1. After intervention with the compound moxa sticks in Example 2, the serum AMH level of female mice with diminished ovarian reserve recovered to 2642.75±201.38 pg / mL, the rate of developing follicles reached 0.72±0.02%, and the oocyte maturation rate reached 83.64±0.56%; the sperm motility of male mice with asthenospermia improved to 67.89±7.63%, the sperm curvilinear velocity (VCL) reached 79.86±11.57 μm / s, and the sperm linear velocity (VSL) reached 32.74±8.62 μm / s.

[0069] The above results indicate that Example 2, by optimizing the moxa wool preparation process and through precise control of moisture regain, multi-stage air-separation fiber grading, and low-temperature sieving to remove impurities, achieved precise control of moxa wool fiber length, significantly increasing the proportion of qualified fibers in the 3-8mm range. Fibers in this length range can form a stable three-dimensional porous structure, providing sufficient attachment sites for the fine powder of traditional Chinese medicine. This effectively solved the problems of uneven fiber length and unstable pore structure in the conventional moxa wool preparation process of Example 1, which led to easy stratification and shedding of medicinal powder and uneven content of medicinal components in different parts of the moxa stick. It ensured the uniformity and stability of the release of medicinal components during the burning of the moxa stick. Compared to Example 1, the compound moxa stick prepared in Example 2 showed superior improvement in the reproductive function of male and female model mice. Serum AMH levels, follicle development rate, and oocyte maturation rate in female mice were significantly increased, approaching the normal physiological levels of the blank control group. This demonstrates a more effective ability to restore core hormones related to ovarian reserve, inhibit follicular atresia, promote normal follicle development, and improve oocyte maturation quality. Simultaneously, the improvement effect on sperm motility and motility parameters in male mice was more stable, with sperm motility, VCL, and VSL essentially returning to the levels of the blank control group, achieving effective improvement in sperm motility and fertilization potential. Furthermore, the intra-group relative standard deviation (RSD) of all test indicators in Example 2 was significantly lower than that in Example 1, confirming that optimizing the moxa wool preparation process alone can significantly improve the batch-to-batch quality stability and reproducibility of the intervention effect of the compound moxa stick.

[0070] Example 3 This embodiment provides a compound moxa stick with a warming and tonifying formula. Based on embodiment 2, after collecting the initial selected moxa wool and before vibrating sieving, the following steps are included: the initial selected moxa wool is evenly spread on a breathable mesh belt and placed in a closed environment with a temperature of 18 ℃ and a relative humidity of 60%. It is continuously sprayed with deionized water with an atomized particle size of 15 μm for 45 min to stabilize the overall moisture content of the moxa wool at 12%. The humidified initial selected moxa wool is fed into a roller loosening machine and subjected to two consecutive roller pressing loosening processes using rollers with a roller gap of 3 mm and an arc-shaped protrusion on the surface to break up the fiber clumps inside the moxa wool and obtain moxa wool in a monofilament dispersed state. The monofilament dispersed moxa wool is fed into a high-voltage electrostatic separator, and the separation electric field strength is controlled at 100kV / m to separate and remove ligninized fibers, hard debris, and non-fibrous impurities from the moxa wool to obtain purified moxa wool. The purified moxa wool is fed into a vibrating screen for subsequent sieving processing.

[0071] Effect test 1. Fiber clumping rate detection: The proportion of clumped fibers is determined by standard vibrating sieve method to characterize the dispersion of Artemisia fibers.

[0072] 2. Testing steps: Sampling: Three parallel samples were randomly selected from the moxa wool to be tested, and each sample was accurately weighed to 10.0 g.

[0073] Sieving: Place the sample in a standard sieve with a 4 mm aperture, use a national standard vibrating sieve machine, set the vibration frequency to 200 times / min and the amplitude to 5 mm, and vibrate horizontally for 2 minutes to allow the loose monofilament fibers to pass through the sieve.

[0074] Weighing: Collect the residue on the sieve (i.e., the clumps of fiber that did not pass through the sieve) and weigh it accurately to a depth of 0.001g.

[0075] Calculation: Agglomerate mass percentage = mass of material on sieve / total sample mass × 100%.

[0076] Parallel verification: The relative deviation of the agglomeration mass percentage of the three samples should be ≤10%.

[0077] Note: This method treats fibers that "cannot pass through a 4 mm sieve" as clumps, thus avoiding subjective judgment differences.

[0078] 3. Serum AMH, follicle rate, oocyte maturation rate in female mice, and sperm motility, VCL, and VSL in male mice were also measured.

[0079] The moxa fiber clumping rate in this embodiment was 3.18±0.42%, which was significantly lower than the 12.35±1.27% clumping rate in Example 2 without this step. Pharmacodynamic verification was performed using the same animal experimental protocol as in Examples 1 and 2. The results showed that after intervention with the compound moxa sticks in Example 3, the serum AMH level in female mice with diminished ovarian reserve recovered to 2712.46±168.52 pg / mL, the rate of developing follicles reached 0.74±0.02%, and the oocyte maturation rate reached 85.37±0.48%. In male mice with asthenospermia, sperm motility increased to 68.19±7.24%, the sperm velocity curve (VCL) reached 80.42±11.36 μm / s, and the velocity linear (VSL) reached 32.58±8.31 μm / s.

[0080] The above results indicate that this embodiment, through the newly added low-temperature atomization humidification process, precisely controls the moisture content of the moxa wool, effectively eliminating static electricity generated on the fiber surface due to mechanical friction. Combined with a roller-type unwinding process, the tangled moxa wool fibers are dispersed into uniform monofilaments. Then, high-voltage electrostatic sorting, based on differences in dielectric constant, precisely removes lignocellulose and hard debris. This fundamentally solves the secondary technical problem of initial moxa wool fiber clumping and impurity inclusion, preventing the formation of a uniform and continuous three-dimensional porous structure, as seen in Embodiment 2. It significantly reduces the clumping rate of moxa wool fibers, resulting in a more uniform and stable porous structure. This not only ensures that the subsequent vibrating sieving process can thoroughly remove residual dust and debris, further improving the purity of the moxa wool, but also allows the fine powder of traditional Chinese medicine to be more evenly embedded in the gaps between the moxa wool fibers, preventing powder stratification and loss, and ensuring the efficacy of the medicine in different parts of the moxa stick is maintained. The uneven distribution of moxa sticks ensures the continuous and stable release of medicinal components during combustion. Compared to Example 2, the compound moxa sticks prepared in this example further enhance the improvement effect on the reproductive function of male and female model mice. The serum AMH level, follicle rate, and oocyte maturation rate of female mice are closer to the normal physiological levels of the blank control group. This can more effectively restore the levels of core markers of ovarian reserve function, reduce follicle atresia, promote normal follicle development, and improve the maturation quality of oocytes. At the same time, the sperm motility, VCL, and VSL of male mice have basically recovered to the levels of the blank control group. The improvement effect on sperm motility and fertilization potential is more stable, and the intra-group dispersion of various indicators is lower. This confirms that the process optimization can further improve the efficacy stability, batch consistency, and clinical intervention effect of the compound moxa sticks.

[0081] Example 4 This embodiment provides a compound moxa stick with a warming and tonifying formula. Based on embodiment 3, after obtaining the purified moxa wool and before sending it to the vibrating screen for sieving, the following steps are added: the purified moxa wool is evenly spread on a breathable mesh belt, with the thickness of the spread material controlled at 8 mm, and placed in a constant flow ventilation environment with a temperature of 20 ℃ and a relative humidity of 40%, with the ventilation speed controlled at 1.5 m / s and the treatment time at 30 min, so that the overall moisture content of the moxa wool is stabilized at 9%; the dehumidified moxa wool is sent into a roller-type static eliminator with a polytetrafluoroethylene insulating layer on the surface, with the roller speed controlled at 20 r / min, the ion air pressure inside the roller at 0.15 MPa, and the treatment time at 15 min, to eliminate residual static electricity on the surface of the moxa wool fibers; the static-eliminated moxa wool is passed through a magnetic separation channel with a magnetic field strength of 0.4 T to remove metal debris mixed in with the moxa wool; the processed moxa wool is sent into the vibrating screen for subsequent sieving.

[0082] Effect test 1. Metal debris content detection: A strong magnet is used to adsorb ferromagnetic metal debris from the moxa wool. The metal debris content is quantitatively calculated by weighing the increase in weight of the magnet or the loss in weight of the moxa wool before and after adsorption.

[0083] 2. Testing steps: Sampling: Randomly select 3 parallel samples from the moxa wool to be tested, weigh each sample precisely 100.0g, accurate to 0.001g, and accurately weigh the sample mass msample.

[0084] Sample laying: Spread the sample evenly on a clean white A3 paper, with a thickness not exceeding 5 mm, ensuring that the fibers are loose and do not overlap.

[0085] Magnetic separation: Use a neodymium iron boron strong magnet (magnetic field strength 0.4 T) wrapped with plastic wrap to slowly move over the laid mugwort surface, about 1-2 mm away from the surface, and cover the entire area along an S-shaped path. Repeat 5 times to allow the magnet to fully attract metal debris.

[0086] Note: Wrap the magnet with plastic wrap to facilitate the separation of metal fragments and avoid magnet contamination.

[0087] Collection: Carefully remove the plastic wrap and transfer the adsorbed metal fragments to pre-weighed weighing paper or aluminum foil using a soft brush or tweezers. Accurately weigh the mass of the metal fragments, m_metal, and record it to 0.0001 g (microbalance).

[0088] Parallel verification: The relative deviation of the metal debris content of the three samples should be ≤15%; otherwise, resampling and testing should be performed.

[0089] Calculation of results: Metal debris content (%) = Metal debris mass / Sample mass × 100%.

[0090] 3. Serum AMH, follicle rate, oocyte maturation rate in female mice, and sperm motility, VCL, and VSL in male mice were also measured.

[0091] In this embodiment, the metal debris content in the moxa wool was 0.0009±0.0002%, which is significantly lower than the 0.0082±0.0015% metal debris content in the moxa wool without this additional process in Example 3. This embodiment basically achieves complete removal of metal debris. Pharmacodynamic verification was performed using the same animal experimental protocol as the aforementioned embodiments. The results showed that after intervention with the compound moxa sticks in this embodiment, the serum AMH level of female mice with diminished ovarian reserve recovered to 2741.58±156.73 pg / mL, the rate of developing follicles reached 0.75±0.02%, and the oocyte maturation rate reached 86.12±0.35%; the sperm motility of male mice with asthenospermia increased to 68.23±7.31%, the sperm curvilinear velocity (VCL) reached 80.57±11.62 μm / s, and the linear velocity (VSL) reached 32.45±8.39 μm / s. The above results show that, through the newly added low-temperature constant flow ventilation process, this embodiment accurately adjusts the moisture content of moxa wool to the optimal processing range of 8%-10% without damaging the volatile effective components of moxa wool. This solves the problems of screen clogging, fiber adhesion, and moisture absorption and degradation of volatile components of traditional Chinese medicine caused by the high moisture content of moxa wool after atomization humidification in Example 3, and also creates the best conditions for the subsequent electrostatic elimination process. Combined with the roller-type ion wind electrostatic elimination treatment, residual static electricity on the surface of moxa wool fibers is completely removed without dead angles, avoiding secondary technical problems such as fiber agglomeration, uneven mixing of medicinal powder, and inconsistent compactness of moxa sticks in the subsequent screening, mixing, and rolling processes from the source. The constant magnetic field magnetic separation process efficiently removes trace metal debris introduced during mechanical processing, completely eliminating the safety hazards of pollutants released by the burning of moxa sticks and damage to production equipment, and further improving the purity and safety of moxa wool. Compared to Example 3, the compound moxa stick prepared in this example has an effect on improving the reproductive function of male and female model mice that is basically on par with the normal physiological level of the blank control group. The serum AMH level, follicle rate and oocyte maturation rate of female mice have further approached those of the blank group. It can restore the core hormone level of ovarian reserve function to the greatest extent, inhibit follicle atresia, promote normal follicle development and improve oocyte maturation quality. At the same time, the sperm motility, VCL and VSL of male mice have completely recovered to the level of the blank control group. The effect on improving sperm motility and fertilization potential is more stable. Moreover, the relative standard deviation (RSD) of each efficacy index within the group has been further reduced compared with Example 3. This confirms that the process optimization has not only greatly improved the safety of use and the environmental friendliness of the preparation process of the compound moxa stick, but also achieved a comprehensive improvement in batch-to-batch quality consistency, drug release stability and clinical intervention effect.

[0092] Example 5 This embodiment provides a compound moxa stick with a warming and unblocking formula. Based on embodiment 4, the corresponding materials are weighed according to a mass ratio of moxa wool to fine Chinese medicine powder of 1:0.5, placed in a mixing container, and continuously stirred and turned to evenly disperse the fine Chinese medicine powder in the moxa wool, resulting in a mixed moxa wool. The specific steps include: grounding the inside of the mixing container for anti-static treatment, controlling the temperature of the mixing environment to 20 ℃ and the relative humidity to 45%; weighing the finished moxa wool and fine Chinese medicine powder according to a mass ratio of 1:0.5, placing all the weighed finished moxa wool in the mixing container, starting the stirring mechanism of the mixing container, and stirring at a low speed of 8 r / min to form a loose and suspended fiber bed of moxa wool; dividing the weighed fine Chinese medicine powder into 4 equal parts, using anhydrous ethanol atomized gas with a particle size of 10 μm as the carrier gas, and mixing each part of fine Chinese medicine powder at 0.75 The powder is uniformly fed into the fluffy fiber bed in the mixing container at a rate of kg / min. After each batch of fine Chinese medicine powder is fed, it is continuously stirred at a speed of 8 r / min for 4 minutes before feeding the next batch. After all the fine Chinese medicine powder has been fed, the mixing environment parameters are kept constant, and it is continuously stirred at a speed of 10 r / min for 10 minutes to obtain a premixed fluff. The premixed fluff is then fed into a double-roller press with a roller gap of 6 mm for one low-pressure rolling. The rolling pressure is controlled at 0.2 MPa to embed the fine Chinese medicine powder into the three-dimensional pore structure of the mugwort fibers, thus obtaining a mixed mugwort and herbal fluff.

[0093] Effect test 1. Definition of uniformity of powder mixing: It characterizes the uniformity of the distribution of fine powder of Chinese medicine in mugwort floss. It is usually expressed as the relative standard deviation (RSD) of the content of characteristic components in samples from different parts. The smaller the RSD, the more uniform the mixing.

[0094] Test method: Detection of uniformity of powder mixing: Samples are taken at different spatial locations of the mixed mugwort floss, and the content of a representative component (such as α-cyperone) is determined. The degree of variation between samples is calculated.

[0095] Testing steps: Sampling: From the mixed mugwort floss after mixing, a total of 9 points (3×3 grid) were taken at the top, middle and bottom layers and the left, middle and right horizontal positions, with each point taking about 5 g of sample.

[0096] If it is a small mixing container, it can be simplified to take 1 portion from each of the top, middle and bottom layers, for a total of 3 portions.

[0097] Sample pretreatment: After thoroughly mixing each sample, accurately weigh 3 parallel subsamples, each approximately 1.0 g, and record the mass.

[0098] Determination of characteristic component content: Using α-cyperone, a specific characteristic component of fine powder of traditional Chinese medicine, as the detection index, the content of α-cyperone in each sample was determined by high performance liquid chromatography (HPLC).

[0099] Sample preparation: Accurately weigh 2.0 g of each sample and place it in a stoppered conical flask. Add 25 mL of methanol, weigh the sample, sonicate (power 250 W, frequency 40 kHz) for 30 min, cool, add weight, shake well, filter, and collect the filtrate to obtain the test solution.

[0100] Calculation method: The content of α-cyperone in each sample was calculated by external standard method, and the relative standard deviation (RSD) of the contents of the 9 sampling points was calculated. RSD (%) = standard deviation (SD) / mean × 100%.

[0101] 2. Serum AMH, follicle rate, oocyte maturation rate of female mice, and sperm motility, VCL, and VSL of male mice were detected simultaneously.

[0102] The results showed that the relative standard deviation (RSD) of the mixed moxa wool prepared in this embodiment was 2.17±0.36%, which was calculated by measuring the content of α-cyperone using a 3×3 grid multi-point sampling method. This was significantly lower than the RSD of 11.82±1.05% obtained by conventional stirring and mixing process in Example 4. The uniformity of the distribution of the medicinal powder in the moxa wool was improved by an order of magnitude.

[0103] The concurrent pharmacodynamic validation results showed that after intervention with the compound moxa sticks in this embodiment, the serum AMH level in female mice with diminished ovarian reserve recovered to 2748.62±148.35 pg / mL, the rate of developing follicles reached 0.76±0.02%, and the oocyte maturation rate reached 86.74±0.31%. In male mice with asthenospermia, sperm motility increased to 68.24±7.26%, the sperm curved velocity (VCL) reached 80.61±11.58 μm / s, and the linear velocity (VSL) reached 32.43±8.42 μm / s. μm / s; The above results show that this embodiment, through the refined optimization of the entire process of the moxa-medicine mixing process, and the systematic process design of grounded anti-static environmental control, low-speed stirring to form a loose suspended fiber bed, anhydrous ethanol atomized airflow as carrier gas for batch feeding, and staged stirring combined with low-pressure roller pressing and fixing, fundamentally solves the core technical problems existing in conventional mixing processes, such as damage to the long fiber structure of moxa wool, electrostatic agglomeration and adsorption of medicinal powder on the container wall, gravity stratification of medicinal powder of different densities, and large deviation between the actual load ratio of medicinal powder and the set ratio. It not only achieves uniform dispersion and stable fixing of fine Chinese medicine powder in the three-dimensional porous structure of moxa wool, but also completely avoids the problems of medicinal powder stratification and shedding during subsequent processing and storage, and uneven distribution of medicinal components in different parts of a single moxa stick. It ensures the constant rate and stable release of medicinal components throughout the burning process of the moxa stick, and maximizes the synergistic effect of multiple medicinal materials in the compound formulation. Compared with Example 4, this embodiment has The pharmacodynamic indicators of the compound moxa sticks prepared in the examples further approached the normal physiological levels of the blank control group. Among them, the serum AMH level, follicle rate, and oocyte maturation rate of female mice were basically the same as those of the blank group, which could completely reverse the cyclophosphamide-induced ovarian reserve function damage, effectively inhibit follicle atresia, promote normal follicle development, and improve oocyte maturation quality. At the same time, the sperm motility, VCL, and VSL of male mice were completely restored to the normal levels of the blank control group, and the improvement effect on sperm motility and fertilization potential was more stable. Moreover, the intra-group relative standard deviation (RSD) of all test indicators was significantly lower than that of the aforementioned examples, which confirms that the optimization of the mixing process not only further improved the batch-to-batch quality consistency and drug release stability of the compound moxa sticks, but also achieved a comprehensive improvement in the reproductive function improvement effect of both male and female products, providing a solid process guarantee for the reproducibility and stability of the clinical intervention effect of the compound moxa sticks.

[0104] Although embodiments of the present invention have been disclosed above, they are not limited to the applications listed in the specification and embodiments. They can be applied to various fields suitable for the present invention. For those skilled in the art, other modifications can be easily made. Therefore, without departing from the general concept defined by the claims and their equivalents, the present invention is not limited to the specific details and illustrations shown and described herein.

Claims

1. A compound moxa stick containing warming and unblocking formula, characterized in that, It is composed of the following raw material components in parts by weight: 15-20 parts of Artemisia argyi, 0.5-1.0 parts of Cyperus rotundus, 0.5-1.0 parts of Alpinia galanga, 0.5-1.0 parts of Cinnamomum cassia, 0.1-0.5 parts of Zingiber officinale, 0.1-0.5 parts of Star anise, 0.1-0.5 parts of Cinnamomum cassia twig, 0.5-1.0 parts of Spatholobus suberectus, 0.1-0.5 parts of Caesalpinia sappan, 0.1-0.5 parts of Moghania macrophylla, 0.1-0.5 parts of Euonymus fortunei, 0.5-1.0 parts of Citronella foenum-graecum, 0.1-0.5 parts of Amomum villosum, 0.5-1.0 parts of Citrus reticulata peel, and 0.1-0.5 parts of Atractylodes lancea.

2. A method for preparing a compound moxa stick containing a warming and unblocking formula, characterized in that, Includes the following steps: Weigh out 15-20 parts by weight of Artemisia argyi, 0.5-1.0 parts of Cyperus rotundus, 0.5-1.0 parts of Alpinia galanga, 0.5-1.0 parts of Cinnamomum cassia, 0.1-0.5 parts of Zingiber officinale, 0.1-0.5 parts of Star anise, 0.1-0.5 parts of Cinnamomum cassia twig, 0.5-1.0 parts of Spatholobus suberectus, 0.1-0.5 parts of Caesalpinia sappan, 0.1-0.5 parts of Moghania macrophylla, 0.1-0.5 parts of Euonymus fortunei, 0.5-1.0 parts of Citrus medica, 0.1-0.5 parts of Amomum villosum, 0.5-1.0 parts of Citrus reticulata peel, and 0.1-0.5 parts of Atractylodes lancea. All raw materials except mugwort leaves are placed in a grinder and ground. After grinding, the powder is passed through a 150-mesh sieve to obtain fine powder of traditional Chinese medicine. Artemisia leaves are processed into Artemisia floss with a ratio of 5-10:1; Weigh the corresponding materials according to the mass ratio of moxa floss to fine Chinese medicine powder of 1:0.5-5, place them in a mixing container and stir continuously to make the fine Chinese medicine powder evenly dispersed in the moxa floss, so as to obtain the mixed moxa floss and medicine. The mixture of medicinal and moxa wool was weighed quantitatively according to the fixed filling amount of a single moxa stick; Mulberry bark paper is used as the paper for rolling. The quantitatively measured mixture of medicinal moxa wool is placed evenly in the moxa stick rolling machine, and the rolling machine is started to complete the rolling, resulting in a moxa stick blank with a diameter of 1.8-2.2 cm and a length of 19-21 cm. The joints and end faces of the moxa stick blank are sealed with special sealing adhesive for moxa sticks; The sealed moxa stick blank is placed in a cool and ventilated place to air dry naturally, resulting in the finished compound moxa stick.

3. The preparation method of the compound moxa stick with warming and unblocking properties according to claim 2, characterized in that, The process of processing mugwort leaves into mugwort floss with a ratio of 5-10:1 includes the following steps: Take aged mugwort leaves that have been stored for 24-36 months and place them in a sealed environment at a temperature of 20-25 ℃ and a relative humidity of 40%-50% for 12-24 hours to allow the moisture content of the mugwort leaves to stabilize at 8%-12%. After the mugwort leaves have been rehydrated, they are fed into a hammer mill and coarsely crushed using a screen with a mesh diameter of 8-10 mm. The hard stems and petioles of the mugwort leaves are separated and removed to obtain coarse mugwort floss. The coarse Artemisia flakes are fed into a multi-stage air classifier unit and passed through air classification channels with progressively decreasing wind speeds of 3-5 levels. The wind speed in the first air classification channel is controlled at 6-8 m / s, and the wind speed in each subsequent air classification channel is reduced by 0.8-1.2 m / s compared to the previous level. After air classification and separation, the flakes with fiber lengths in the range of 3-8 mm are collected to obtain the initial selected Artemisia flakes. The initial selected mugwort floss is placed in an environment with a temperature of 15-20 ℃ and sieved using a 40-60 mesh vibrating screen to remove the mugwort leaf dust and short floss fragments that are sieved. The material on the sieve is collected to obtain the finished mugwort floss with a floss extraction ratio of 5-10:

1.

4. The preparation method of the compound moxa stick with warming and unblocking properties according to claim 3, characterized in that, After collecting the initial selected moxa wool and before vibrating sieving, the following steps are also included: The selected moxa wool is evenly spread on a breathable mesh belt and placed in a closed environment with a temperature of 15-20 ℃ and a relative humidity of 55%-65%. It is continuously sprayed with deionized water with an atomization particle size of 10-20 μm for humidification for 30-60 minutes to stabilize the overall moisture content of the moxa wool at 10%-14%. The humidified and initially selected mugwort floss is fed into a roller loosening machine. The rollers with a gap of 2-4 mm and arc-shaped protrusions on the surface are used to continuously roll and loosen the floss 2-3 times to break up the fiber clumps inside the mugwort floss and obtain mugwort floss in a monofilament dispersed state. The monofilament dispersed mugwort floss is fed into a high-voltage electrostatic separator, and the separation electric field strength is controlled at 80-120kV / m to separate and remove lignocellulose, hard debris and non-fibrous impurities from the mugwort floss, thus obtaining purified mugwort floss. The purified mugwort floss is fed into a vibrating screen for subsequent sieving processing.

5. The preparation method of the compound moxa stick with warming and unblocking properties according to claim 4, characterized in that, After obtaining the purified Artemisia floss and before it is fed into a vibrating screen for sieving, the following steps are also included: The purified moxa wool is evenly spread on a breathable mesh belt, with the thickness of the spread material controlled at 5-10 mm. It is placed in a constant flow ventilation environment with a temperature of 18-22 ℃ and a relative humidity of 35%-45%, with the ventilation speed controlled at 1-2 m / s and the treatment time at 20-40 min, so that the overall moisture content of the moxa wool is stabilized at 8%-10%. The dehumidified mugwort floss is fed into a roller-type static eliminator with a polytetrafluoroethylene insulating layer on the surface. The roller speed is controlled at 15-25 r / min, the ion air pressure inside the roller is 0.1-0.2 MPa, and the treatment time is 10-20 min to eliminate the residual static electricity on the surface of the mugwort floss fibers. After static electricity is eliminated, the moxa wool is passed through a magnetic separation channel with a magnetic field strength of 0.3-0.5 T to remove metal debris mixed in with the moxa wool; The processed mugwort floss is fed into a vibrating screen for subsequent screening.

6. The preparation method of the compound moxa stick with warming and unblocking properties according to claim 2, characterized in that, Weigh the corresponding materials according to the mass ratio of moxa wool to fine Chinese medicine powder of 1:0.5-5, place them in a mixing container and stir continuously to evenly disperse the fine Chinese medicine powder in the moxa wool, thus obtaining a mixed moxa wool and Chinese medicine powder. The specific steps include: The interior of the mixing container should be grounded to prevent static electricity, and the temperature of the mixing operation environment should be controlled at 18-22 ℃, and the relative humidity at 40%-50%. Weigh out the finished moxa floss and fine Chinese medicine powder at a mass ratio of 1:0.5-5. Place all the weighed finished moxa floss into a mixing container, start the mixing container's stirring mechanism, and stir at a low speed of 5-10 r / min to make the moxa floss form a fluffy, suspended fiber bed. Divide the weighed Chinese herbal powder into 3-5 equal portions. Use anhydrous ethanol atomized gas with a particle size of 5-15 μm as the carrier gas. Evenly convey each portion of Chinese herbal powder to the loose fiber bed in the mixing container at a rate of 0.5-1.0 kg / min. After conveying each portion of Chinese herbal powder, maintain a speed of 5-10 r / min and continue to stir for 3-5 min before conveying the next portion of Chinese herbal powder. After all the fine powder of Chinese medicine has been transported, the mixing environment parameters are kept constant, and the mixture is continuously stirred at a speed of 8-12 r / min for 8-12 min to obtain a premixed velvet. The premixed floss is fed into a double-roll press with a roller gap of 5-8 mm and subjected to low-pressure rolling once or twice, so that the fine powder of traditional Chinese medicine is embedded in the three-dimensional porous structure of the mugwort floss fiber to obtain the mixed floss of medicine and mugwort.

7. The application of the warming and unblocking compound moxa stick as described in claim 1 in the preparation of moxibustion intervention products for improving the reproductive function of mammals.

8. The application according to claim 7, characterized in that, Improving reproductive function in mammals specifically means improving ovarian reserve in female mammals or improving asthenospermia in male mammals.

9. Application of moxa wool in the preparation of moxibustion products that improve ovarian reserve function in female mammals.

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