Method for detecting and distinguishing FFCA, DFF and HMFCA through liquid chromatography

By optimizing the combination of chromatographic conditions and mobile phase, and using a specific chromatographic column and acidic mobile phase, baseline separation of FFCA, DFF and HMFCA was achieved, solving the problem of simultaneous separation and detection in existing technologies, and making it suitable for monitoring biomass conversion reactions.

CN122361652APending Publication Date: 2026-07-10NINGBO HUAFU NEW MATERIAL TECH CO LTD +1
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Patent Information

Application Number
CN202610464572.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-04-09
Publication Date
2026-07-10

AI Technical Summary

Technical Problem

Existing technologies make it difficult to simultaneously separate and detect three substances with similar structures, FFCA, DFF, and HMFCA, under the same chromatographic conditions, and conventional chromatographic conditions are insufficient to effectively distinguish between the three.

Method used

Using specific chromatographic columns (such as octadecylsilane-bonded silica gel or pentafluorophenyl columns) and acidic mobile phases (such as potassium dihydrogen phosphate solution or acetonitrile/methanol system), combined with isocratic or gradient elution techniques, chromatographic conditions are optimized to achieve baseline separation of the three components.

Benefits of technology

Baseline separation of FFCA, DFF and HMFCA was achieved under the same chromatographic conditions, with a resolution greater than 1.5, meeting analytical requirements and suitable for reaction monitoring in the field of biomass conversion.

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Abstract

The invention discloses a method for detecting and distinguishing FFCA, DFF and HMFCA through liquid chromatography. Belongs to the technical field of analytical chemistry and pharmaceutical analysis, the structure can simultaneously separate and detect three furan derivatives with similar structures, namely FFCA, 5-formyl-2-furancarboxylic acid, DFF (2, 5-diformyl furan) and HMFCA (5-hydroxymethyl-2-furancarboxylic acid) under the same chromatographic condition, and the separation degrees among the three furan derivatives are all greater than 1.5. A method for detecting and distinguishing FFCA, DFF and HMFCA through liquid chromatography is characterized in that an adopted chromatographic column is an octadecyl silane bonded silica gel chromatographic column or a pentafluorophenyl chromatographic column; eluting by adopting a mobile phase A and a mobile phase B as a mixed mobile phase; the mobile phase A is an acidic aqueous solution; the mobile phase B is acetonitrile or methanol; according to the method, the FFCA, the DFF and the HMFCA are separated at the same time under the same chromatographic condition, and the separation degrees of the FFCA, the DFF and the HMFCA are all larger than 1.5.
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