A method for detecting and distinguishing ffca, dff, hmfca by liquid chromatography

By optimizing the combination of chromatographic conditions and mobile phase, and using a specific chromatographic column and acidic mobile phase, baseline separation of FFCA, DFF and HMFCA was achieved, solving the problem of simultaneous separation and detection in existing technologies, and making it suitable for monitoring biomass conversion reactions.

CN122361652APending Publication Date: 2026-07-10NINGBO HUAFU NEW MATERIAL TECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NINGBO HUAFU NEW MATERIAL TECH CO LTD
Filing Date
2026-04-09
Publication Date
2026-07-10

AI Technical Summary

Technical Problem

Existing technologies make it difficult to simultaneously separate and detect three substances with similar structures, FFCA, DFF, and HMFCA, under the same chromatographic conditions, and conventional chromatographic conditions are insufficient to effectively distinguish between the three.

Method used

Using specific chromatographic columns (such as octadecylsilane-bonded silica gel or pentafluorophenyl columns) and acidic mobile phases (such as potassium dihydrogen phosphate solution or acetonitrile/methanol system), combined with isocratic or gradient elution techniques, chromatographic conditions are optimized to achieve baseline separation of the three components.

Benefits of technology

Baseline separation of FFCA, DFF and HMFCA was achieved under the same chromatographic conditions, with a resolution greater than 1.5, meeting analytical requirements and suitable for reaction monitoring in the field of biomass conversion.

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Abstract

The application discloses a method for distinguishing FFCA, DFF and HMFCA by liquid chromatography. The method belongs to the technical field of analytical chemistry and pharmaceutical analysis, and the structure can separate and detect three furan derivatives of FFCA, 5-formyl-2-furancarboxylic acid, DFF (2,5-diformylfuran) and HMFCA (5-hydroxymethyl-2-furancarboxylic acid) under the same chromatographic condition, and the resolution between the three is greater than 1.5. The method for distinguishing FFCA, DFF and HMFCA by liquid chromatography is characterized in that: an octadecylsilane bonded silica gel chromatographic column or a pentafluorophenyl chromatographic column is used; a mixed mobile phase of mobile phase A and mobile phase B is used for elution; the mobile phase A is an acidic aqueous solution; the mobile phase B is acetonitrile or methanol; the method simultaneously separates FFCA, DFF and HMFCA under the same chromatographic condition, and the resolution between the three is greater than 1.5.
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Citation Information

Patent Citations

  • A method for preparing 2,5-furandicarboxaldehyde from 5-hydroxymethylfurfural

    CN111072601B