A composition for preventing or eliminating stretch marks, and a preparation method and application thereof

Abstract

The present application provides a kind of composition for preventing or eliminating striae gravidarum and its preparation method and application, belong to the field of cosmetics.The present application composition includes the following raw materials by mass fraction: Rana chensinensis skin elastic polypeptide 3-5 parts, soybean oligopeptide 2-4 parts, Ganoderma lucidum polysaccharide extract 2-3 parts, sea buckthorn fruit oil extract 1.5-2.5 parts, calendula total flavonoid extract 1-2 parts, marula oil 4-6 parts and acacia vera oil 3-5 parts.The present application can significantly promote human dermal fibroblast proliferation, improve I / III collagen and elastin synthesis, and efficiently inhibit MMP-9 activity;Human trials show that the present application cream can effectively improve skin elasticity, reduce the area of striae gravidarum, fade red striae and redness, and improve rough skin, and is mild and non-irritating, suitable for sensitive skin during pregnancy and postpartum, with dual effects of prevention and repair.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, and in particular relates to a composition for preventing or eliminating stretch marks, its preparation method, and its application. Background Technology

[0002] Stretch marks are caused by hormonal changes and rapid abdominal expansion during pregnancy, which lead to excessive stretching and damage to the collagen and elastic fibers in the dermis of the skin. As a result, they form purplish-red or silvery-white atrophic lines on the abdomen, buttocks, thighs, and other areas. Once formed, they are difficult to disappear on their own and seriously affect the appearance of the skin and the user's physical and mental health.

[0003] Currently, most products on the market that prevent and repair stretch marks are based on single moisturizing and skin-nourishing ingredients, which can only relieve dry skin and cannot target and repair the damaged dermal fiber network. Some products have added functional ingredients but have problems such as poor transdermal absorption, unstable activity, and weak anti-inflammatory and repair effects, making it difficult to promote collagen and elastin regeneration and inhibit the degradation of fibers by matrix metalloproteinases from the root. Some products also contain irritating ingredients and are not suitable for sensitive skin during pregnancy and postpartum, posing a safety risk.

[0004] Therefore, developing a cosmetic product that can target and repair skin fibers, efficiently promote collagen synthesis, is gentle and safe, and has the effect of preventing and eliminating stretch marks has become a technical problem that urgently needs to be solved in this field. Summary of the Invention

[0005] In view of this, the purpose of the present invention is to provide a composition for preventing or eliminating stretch marks, a method for preparing the composition and its application, which can significantly promote the synthesis of elastin and collagen in the skin, inhibit the activity of matrix metalloproteinases, relieve redness and itching of stretch marks, and improve skin elasticity.

[0006] To achieve the above-mentioned objectives, the present invention provides the following technical solution: A composition for preventing or eliminating stretch marks, comprising the following ingredients in parts by weight: The ingredients include 3-5 parts of forest frog skin elastic peptides, 2-4 parts of soybean oligopeptides, 2-3 parts of Ganoderma lucidum polysaccharide extract, 1.5-2.5 parts of sea buckthorn fruit oil extract, 1-2 parts of calendula total flavonoid extract, 4-6 parts of marula oil, and 3-5 parts of argan oil.

[0007] Preferably, the molecular weight of the frog skin elastic polypeptide is 800-1500 Da; and the molecular weight of the soybean oligopeptide is 500-1200 Da.

[0008] The present invention also provides a method for preparing the above composition, comprising the following steps: The following ingredients were prepared separately: forest frog skin elastic peptides, soybean oligopeptides, Ganoderma lucidum polysaccharide extracts, sea buckthorn fruit oil extracts, and calendula total flavonoid extracts. These were then mixed with marula oil and arugula oil in a specific ratio to obtain the composition.

[0009] Preferably, the preparation steps of the forest frog skin elastic polypeptide are as follows: Raw material pretreatment: Clean and mince the frog skin, add purified water at a material-to-liquid ratio of 1:8 to 1:12, and homogenize; Compound enzymatic hydrolysis: Papain and neutral protease are added at an enzyme activity ratio of 1:1.5 to 1:2.5. Enzymatic hydrolysis is carried out at 40 to 50°C and pH 6.5 to 7.5 for 3 to 5 hours, and enzyme inactivation is carried out at 85 to 95°C for 8 to 12 minutes. Membrane separation: The enzymatic hydrolysate is separated by a 1500 Da ultrafiltration membrane, the permeate is collected, and concentrated under reduced pressure at 50~60℃ and a vacuum of -0.07~-0.09 MPa; Drying: Spray drying to obtain the forest frog skin elastic polypeptide.

[0010] Preferably, the preparation steps of the soybean oligopeptides are as follows: soybean protein is hydrolyzed by alkaline protease, separated by a 1200 Da membrane, and spray-dried to obtain soybean oligopeptides with a molecular weight of 500~1200 Da.

[0011] Preferably, the preparation steps of the Ganoderma lucidum polysaccharide extract are as follows: the fruiting body of Ganoderma lucidum is pulverized and passed through a 30-50 mesh sieve, purified water is added at a material-to-liquid ratio of 1:15-1:25, and ultrasonic extraction is performed at 200-400W and 65-75℃ for 1-2 hours, followed by filtration; the filtrate is concentrated to 1:4-1:6, 2-4 times the volume of 90%-95% ethanol is added, and the mixture is allowed to stand at 2-6℃ for 10-14 hours, centrifuged to collect the precipitate, and then freeze-dried under vacuum. The preparation steps of the sea buckthorn fruit oil extract are as follows: supercritical CO2 extraction, extraction pressure 25~35MPa, temperature 40~50℃, CO2 flow rate 15~25L / h, extraction for 1.5~2.5h; The preparation steps of the total flavonoid extract of calendula are as follows: calendula flowers are crushed, 60% to 80% ethanol is added at a material-to-liquid ratio of 1:10 to 1:20, microwave extraction is performed at 300 to 500W and 55 to 65℃ for 30 to 50 minutes, followed by filtration, concentration under reduced pressure, and vacuum drying.

[0012] The present invention also provides the use of the above composition in the preparation of cream products for preventing or eliminating stretch marks.

[0013] The present invention also provides a cream for preventing or eliminating stretch marks, comprising 15-25 wt% of the above composition, excipients and purified water.

[0014] Preferably, the excipients include oil-phase excipients and aqueous-phase excipients; The oil phase excipients consist of 3-4 wt% cetearyl alcohol, 2-3 wt% glyceryl monostearate, 2-3 wt% caprylic / capric triglyceride, and 0.3-0.5 wt% vitamin E acetate. The aqueous phase excipients are 4-6 wt% glycerol, 1-2 wt% panthenol, 0.1-0.2 wt% sodium hyaluronate, 0.2-0.3 wt% carbomer 940, 0.15-0.25 wt% triethanolamine, 0.15-0.2 wt% methylparaben, and 0.3-0.5 wt% phenoxyethanol.

[0015] The present invention also provides a method for preparing the above-mentioned cream, comprising the following steps: Oil phase preparation: Mix the oil phase additives, heat to 80~85℃ to dissolve, and keep warm; Aqueous phase preparation: Glycerin, panthenol, sodium hyaluronate, carbomer, and methylparaben are added to purified water, dispersed evenly, heated to 80-85℃, and kept at that temperature; Emulsification: The oil phase is slowly added to the water phase, shear emulsification is performed, and the mixture is transferred to a vacuum emulsification pot for gradient cooling; the gradient cooling is 80~85℃→60~65℃, held for 10min→38~40℃, held for 15min. Add active ingredients: Add the composition at 38~40℃ and stir; pH adjustment and discharge: Adjust the pH to 5.5~6.5 with triethanolamine, add phenoxyethanol, stir, and filter to discharge.

[0016] Compared with the prior art, the present invention has the following beneficial effects: This invention utilizes frog skin elastic peptides and soybean oligopeptides, which are small-molecule active peptides with strong transdermal absorption. These peptides directly replenish the skin's elastin and collagen synthesis raw materials, activate fibroblast proliferation, and significantly increase the content of type I / III collagen and elastin. They repair broken elastic and collagen fibers from the root, filling in stretch marks and improving skin laxity. Ganoderma lucidum polysaccharide extract effectively inhibits the activity of matrix metalloproteinase-9, blocking its degradation and damage to collagen and elastic fibers. Calendula total flavonoid extract inhibits inflammatory factors such as IL-6 and TNF-α, quickly relieving redness, itching, and burning during the stretch mark stage, improving telangiectasia, and preventing further deterioration of stretch marks. Marula oil, argan oil, and sea buckthorn fruit oil extracts work synergistically to provide long-lasting moisturizing and water-locking, repair the skin barrier, enhance skin elasticity, and prevent the formation of new stretch marks. All ingredients are natural and gentle, free of fragrances, pigments, and irritating ingredients, making them safe for use on sensitive skin during pregnancy and postpartum.

[0017] This invention employs processes such as enzymatic hydrolysis, ultrasonic / microwave-assisted extraction, and supercritical CO2 extraction to maximize the preservation of raw material activity; the cream uses a gradient cooling emulsification process, resulting in a delicate and stable texture that is easy to apply without pilling, with uniform dispersion of active ingredients and high bioavailability.

[0018] This invention is remarkably effective, superior to commercially available products. In vitro cell experiments show that the composition of this invention can increase the proliferation rate of fibroblasts by 72.58% and inhibit MMP-9 by 70.36%. Human clinical trials show that after 8 weeks of continuous use, the effects of improving total skin elasticity, the reduction rate of stretch mark area, the fading rate of red marks, and the improvement of skin roughness are all significant, demonstrating a definite effect in preventing and eliminating stretch marks. Detailed Implementation

[0019] This invention provides a composition for preventing or eliminating stretch marks, comprising the following ingredients by weight: 3-5 parts of forest frog skin elastic polypeptide, 2-4 parts of soybean oligopeptide, 2-3 parts of Ganoderma lucidum polysaccharide extract, 1.5-2.5 parts of sea buckthorn fruit oil extract, 1-2 parts of calendula total flavonoid extract, 4-6 parts of marula oil, and 3-5 parts of argan oil.

[0020] In this invention, the functions of each component are as follows: Forest frog skin elastic peptides: Target and repair elastic fibers; replenish lost elastin in the skin, enhance the skin's stretching and resilience, and fundamentally improve stretch marks, sagging, and other signs of pregnancy; small molecule peptides easily penetrate the skin, activating fibroblasts to secrete elastic fibers, allowing the broken elastic network to reconnect; improve skin toughness and reduce the probability of skin tearing during pregnancy. This invention preferably uses 4 parts of forest frog skin elastic peptides; further preferably, the molecular weight of the forest frog skin elastic peptides is 800~1500 Da.

[0021] Soybean oligopeptides: provide collagen synthesis raw materials for the dermis, increase the content of type I / III collagen, and fill in wrinkles and depressions; small molecule peptides can open skin penetration channels, helping other active ingredients to enter the dermis more quickly; they have antioxidant properties, reduce free radical damage to fibers, and delay wrinkle aging and whitening. This invention preferably uses 3 parts of soybean oligopeptides, and more preferably, the molecular weight of the soybean oligopeptides is 500~1200 Da.

[0022] Ganoderma lucidum polysaccharide extract: blocks the damage of collagen and elastin fibers by metalloproteinases, preventing stretch marks from worsening; enhances the stability of the dermal structure, improves skin support, and reduces sagging; has anti-inflammatory and soothing properties, reducing sensitivity and redness in stretch mark areas. The preferred amount of Ganoderma lucidum polysaccharide extract in this invention is 2.5 parts.

[0023] Sea buckthorn fruit oil extract: rich in unsaturated fatty acids and vitamins C and E, it quickly repairs the damaged skin barrier caused by stretch marks; relieves burning and itching during the red stretch mark stage, and accelerates the fading of red stretch marks; nourishes dermal cells and improves dry, rough, and cracked skin. The preferred amount of sea buckthorn fruit oil extract in this invention is 2 parts.

[0024] Calendula total flavonoid extract: inhibits IL-6 and TNF-α inflammatory factors, and has a rapid effect on redness and itching during the erythema stage; improves skin capillary dilation and reduces redness of stretch marks; gentle and non-irritating, suitable for sensitive skin during pregnancy / postpartum. The preferred amount of calendula total flavonoid extract in this invention is 1.5 parts.

[0025] Marula oil: It has strong antioxidant properties, protecting fibers from oxidation and breakage; it moisturizes without being greasy, significantly improving skin's elasticity and preventing new stretch marks; it provides long-lasting hydration, relieving dry and tight skin during pregnancy. This invention preferably uses 5 parts marula oil.

[0026] Argan oil: Rich in phytosterols, it repairs the skin barrier and reduces transepidermal water loss; it softens the stratum corneum, smooths out wrinkles, and makes the skin smoother; it is natural and gentle, free of fragrance and pigments, and safe for use during pregnancy. This invention preferably uses 4 parts of argan oil.

[0027] The present invention also provides a method for preparing the above-mentioned composition for preventing or eliminating stretch marks, comprising the following steps: (1) Preparation of elastic polypeptides from frog skin: Raw material pretreatment: The frog skin is washed, minced, and purified water is added at a material-to-liquid ratio of 1:8 to 1:12 to homogenize; the material-to-liquid ratio is further optimized to 1:10.

[0028] Compound enzymatic hydrolysis: Papain and neutral protease are added, with an enzyme activity ratio of 1:1.5 to 1:2.5. The hydrolysis temperature is 40 to 50°C, the pH is 6.5 to 7.5, and the hydrolysis time is 3 to 5 hours. The enzyme is then inactivated at a temperature of 85 to 95°C for 8 to 12 minutes. Further optimization is achieved with an enzyme activity ratio of 1:2, a hydrolysis temperature of 45°C, a pH of 7.0, a hydrolysis time of 4 hours, and an enzyme inactivation temperature of 90°C for 10 minutes.

[0029] Membrane separation: The enzymatic hydrolysate is separated by a 1500 Da ultrafiltration membrane, the permeate is collected, and concentrated under reduced pressure at 50~60℃ and -0.07~-0.09MPa; the concentration temperature is further optimized to be 55℃ and the vacuum degree to be -0.08MPa.

[0030] Drying: Spray drying with an inlet air temperature of 150~170℃ and an outlet air temperature of 75~85℃ to obtain forest frog skin elastic polypeptide (molecular weight 800~1500Da); further preferred inlet air temperature is 160℃ and outlet air temperature is 80℃.

[0031] (2) Preparation of soybean oligopeptides: Soybean protein was hydrolyzed by alkaline protease, separated by a 1200 Da membrane, and spray-dried to obtain soybean oligopeptides with a molecular weight of 500~1200 Da.

[0032] (3) Preparation of Ganoderma lucidum polysaccharide extract: The fruiting bodies of Ganoderma lucidum are crushed and passed through a 30-50 mesh sieve. Purified water is added at a material-to-liquid ratio of 1:15 to 1:25. Further optimization involves passing the fruiting bodies through a 40 mesh sieve and adding water at a material-to-liquid ratio of 1:20.

[0033] Ultrasonic-assisted extraction: ultrasonic power 200~400W, temperature 65~75℃, extraction time 1~2h, filtration; further optimized ultrasonic power 300W, temperature 70℃, extraction time 1.5h.

[0034] Alcohol precipitation: Concentrate the filtrate to 1:4~1:6, add 2~4 times the volume of 90%~95% ethanol, let stand at 2~6℃ for 10~14h, and centrifuge to collect the precipitate; further optimize the concentration ratio to 1:5, the amount of ethanol added to be 3 times the volume and the concentration to be 95%, the standing temperature to be 4℃ and the time to be 12h.

[0035] Drying: Vacuum freeze drying was used to obtain Ganoderma lucidum polysaccharide extract.

[0036] (4) Preparation of sea buckthorn fruit oil: supercritical CO2 extraction: extraction pressure is 25~35MPa, temperature is 40~50℃, CO2 flow rate is 15~25L / h, extraction time is 1.5~2.5h, and sea buckthorn fruit oil is obtained; further optimized extraction pressure is 30MPa, temperature is 45℃, CO2 flow rate is 20L / h, and extraction time is 2h.

[0037] (5) Preparation of total flavonoid extract from calendula: Calendula flowers are pulverized, and 60% to 80% ethanol is added at a material-to-liquid ratio of 1:10 to 1:20; a further preferred material-to-liquid ratio is 1:15, and the ethanol concentration is 70%.

[0038] Microwave-assisted extraction: microwave power of 300~500W, temperature of 55~65℃, extraction time of 30~50min, followed by filtration; further optimized microwave power of 400W, temperature of 60℃, and extraction time of 40min.

[0039] Concentration and drying: Concentrate under reduced pressure and dry under vacuum to obtain total flavonoid extract of calendula.

[0040] (6) Mixing: The following ingredients are added to a vacuum mixer in proportion: the elastic peptides of frog skin, soybean oligopeptides, Ganoderma lucidum polysaccharide extract, sea buckthorn fruit oil extract, total flavonoid extract of calendula, marula oil and aralia oil. The speed is 25~35 rpm and the vacuum degree is -0.05~-0.07 MPa. The mixture is mixed at room temperature for 15~25 min to obtain a uniform composition for preventing or eliminating stretch marks. The speed is further preferred to be 30 rpm, the vacuum degree is -0.06 MPa and the mixing time is 20 min.

[0041] As one possible implementation method, the frog skin, soybean protein, Ganoderma lucidum, sea buckthorn and calendula used in the above preparation method of the present invention, as well as the raw materials marula oil and argan oil, can all be obtained through market channels.

[0042] The present invention also provides the use of the above composition in the preparation of cream products for preventing or eliminating stretch marks.

[0043] The present invention also provides a cream for preventing or eliminating stretch marks, comprising 15-25 wt% of the above-mentioned composition for preventing or eliminating stretch marks, excipients and purified water; preferably, the composition for preventing or eliminating stretch marks is 20 wt%.

[0044] In this invention, the preferred excipients include oil-phase excipients and water-phase excipients.

[0045] Further preferred oil phase excipients are 3-4 wt% cetearyl alcohol, 2-3 wt% glyceryl monostearate, 2-3 wt% caprylic / capric triglyceride, and 0.3-0.5 wt% vitamin E acetate.

[0046] Further preferred aqueous excipients are 4-6 wt% glycerol, 1-2 wt% panthenol, 0.1-0.2 wt% sodium hyaluronate, 0.2-0.3 wt% carbomer 940, 0.15-0.25 wt% triethanolamine, 0.15-0.2 wt% methylparaben, and 0.3-0.5 wt% phenoxyethanol.

[0047] In this invention, the auxiliary materials and their functions are as follows: Cetearyl alcohol and glyceryl monostearate: emulsify, thicken, and stabilize the cream, forming a delicate cream texture that is easy to apply and does not pill; more preferably, cetearyl alcohol is 3.5 wt% and glyceryl monostearate is 2.5 wt%. Caprylic / capric triglyceride: moisturizes and smooths the skin, improves skin feel, and helps the dispersion and penetration of active ingredients; more preferably, caprylic / capric triglyceride is 2.5 wt%. Vitamin E acetate: antioxidant, protects peptides and other active substances from oxidation and inactivation. The above excipients work synergistically to reduce wrinkles and delay skin aging; more preferably, vitamin E acetate is 0.4 wt%.

[0048] Glycerin, panthenol, and sodium hyaluronate: triple moisturizing, deeply hydrating and locking in moisture on the surface, maintaining skin hydration and reducing traction damage. In this invention, glycerin is preferably 5 wt%, panthenol 1.5 wt%, and sodium hyaluronate 0.15 wt%.

[0049] Carbomer 940 and triethanolamine: These adjust the consistency and pH of the cream, making it stable and gentle. More preferably, the present invention uses 0.25 wt% carbomer 940 and 0.2 wt% triethanolamine.

[0050] Methylparaben and phenoxyethanol: mild preservatives that ensure product shelf life, non-irritating, and safe for use during pregnancy. This invention preferably uses 0.18 wt% methylparaben and 0.4 wt% phenoxyethanol.

[0051] After determining the mass concentration of the composition and excipients for preventing or eliminating stretch marks, the present invention adds purified water to 100 wt%.

[0052] As one possible implementation method, the above-mentioned excipients of the present invention can all be obtained through market channels.

[0053] The present invention also provides a method for preparing the above-mentioned cream, comprising the following steps: Oil phase preparation: Mix the oil phase additives, heat to 80~85℃ to dissolve, and keep warm.

[0054] Aqueous phase preparation: Glycerin, panthenol, sodium hyaluronate, carbomer, and methylparaben are added to purified water, dispersed evenly, heated to 80~85℃, and kept warm.

[0055] Emulsification: The oil phase is slowly added to the water phase; shear emulsification is performed, preferably at a speed of 12000 rpm for 5 min; the mixture is then transferred to a vacuum emulsification pot, preferably at a vacuum degree of -0.07 MPa and a stirring speed of 40 rpm; the mixture is then subjected to gradient cooling, from 80~85℃ to 60~65℃, preferably at a cooling frequency of 2℃ / min, and held for 10 min; then to 38~40℃, preferably at a cooling frequency of 1℃ / min, and held for 15 min.

[0056] Add active ingredients: Add the composition at 38~40℃, stir, preferably at 20 rpm for 15 min, until uniform and free of particles.

[0057] pH adjustment and discharge: Adjust the pH to 5.5~6.5 with triethanolamine, add phenoxyethanol and stir, preferably at a speed of 20 rpm for 5 minutes, filter and discharge, preferably with a filter size of 200 mesh.

[0058] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0059] Example 1 A composition for preventing or eliminating stretch marks, the components and preparation method of which are as follows: Four parts of forest frog skin elastic peptides, three parts of soybean oligopeptides, two and a half parts of Ganoderma lucidum polysaccharide extract, two parts of sea buckthorn fruit oil extract, one and a half parts of calendula total flavonoid extract, five parts of marula oil, and four parts of arugula oil.

[0060] Preparation method: (1) Preparation of elastic peptides from frog skin: frog skin was washed and minced, and purified water was added at a material-to-liquid ratio of 1:10 to homogenize the mixture; papain and neutral protease (enzyme activity ratio of 1:2) were added, and the mixture was enzymatically hydrolyzed at 45℃ and pH 7.0 for 4 h, and then the enzyme was inactivated at 90℃ for 10 min; the hydrolysate was separated by a 1500 Da ultrafiltration membrane, concentrated under reduced pressure at 55℃ and -0.08 MPa; and spray-dried at 160℃ for air intake and 80℃ for air exhaust to obtain 800~1500 Da elastic peptides from frog skin.

[0061] (2) Preparation of soybean oligopeptides: Soybean protein was hydrolyzed by alkaline protease, separated by a 1200 Da membrane, and spray-dried to obtain soybean oligopeptides of 500~1200 Da.

[0062] (3) Preparation of Ganoderma lucidum polysaccharide extract: Ganoderma lucidum fruiting body was crushed and passed through a 40-mesh sieve. The material-to-liquid ratio was 1:20 and purified water was added. The extract was ultrasonically extracted at 300W and 70℃ for 1.5h and then filtered. The filtrate was concentrated to 1:5 and 3 times the volume of 95% ethanol was added. The mixture was allowed to stand at 4℃ for 12h, centrifuged to collect the precipitate, and then freeze-dried under vacuum.

[0063] (4) Preparation of sea buckthorn fruit oil extract: supercritical CO2 extraction, pressure 30MPa, temperature 45℃, CO2 flow rate 20L / h, extraction for 2h.

[0064] (5) Preparation of total flavonoid extract of calendula: Calendula flowers were crushed, and 70% ethanol was added at a material-to-liquid ratio of 1:15; microwave extraction was performed at 400W and 60℃ for 40 min, filtered, concentrated under reduced pressure, and vacuum dried.

[0065] (6) Mixing: Put the above raw materials into a vacuum mixer and mix at 30 rpm, -0.06 MPa and room temperature for 20 min to obtain the final product.

[0066] Example 2 A composition for preventing or eliminating stretch marks, the components and preparation method of which are as follows: Three parts of forest frog skin elastic peptides, two parts of soybean oligopeptides, two parts of Ganoderma lucidum polysaccharide extract, one and a half parts of sea buckthorn fruit oil extract, one part of calendula total flavonoid extract, four parts of marula oil, and three parts of arugula oil.

[0067] Preparation method: (1) Forest frog skin elastic polypeptide: material-liquid ratio 1:8, enzyme activity ratio 1:1.5, enzymatic hydrolysis at 40℃ and pH 6.5 for 3h, enzyme inactivation at 85℃ for 8min; 1500Da ultrafiltration membrane, concentration at 50℃ and -0.07MPa; air inlet at 150℃ and air outlet at 75℃ for drying.

[0068] (2) Soybean oligopeptides: Same as in Example 1.

[0069] (3) Ganoderma lucidum polysaccharide: pass through a 30-mesh sieve, with a material-to-liquid ratio of 1:15; ultrasonically extract at 200W and 65℃ for 1 hour; concentrate to 1:4, add 2 times the volume of 90% ethanol, and let stand at 2℃ for 10 hours.

[0070] (4) Sea buckthorn fruit oil: Extracted at 25MPa, 40℃, 15L / h for 1.5h.

[0071] (5) Total flavonoids from calendula: 60% ethanol was added at a material-to-liquid ratio of 1:10; microwave extraction was performed at 300W and 55℃ for 30 min.

[0072] (6) Mixing: Mix at 25 rpm, -0.05 MPa, and room temperature for 15 min. Other steps are the same as in Example 1.

[0073] Example 3 A composition for preventing or eliminating stretch marks, the components and preparation method of which are as follows: Five parts of forest frog skin elastic peptides, four parts of soybean oligopeptides, three parts of Ganoderma lucidum polysaccharide extract, two and a half parts of sea buckthorn fruit oil extract, two parts of calendula total flavonoid extract, six parts of marula oil, and five parts of arugula oil.

[0074] Preparation method: (1) Forest frog skin elastic polypeptide: material-liquid ratio 1:12, enzyme activity ratio 1:2.5, enzymatic hydrolysis at 50℃ and pH 7.5 for 5h, enzyme inactivation at 95℃ for 12min; 1500Da ultrafiltration membrane, concentration at 60℃ and -0.09MPa; air inlet at 170℃ and air outlet at 85℃ for drying.

[0075] (2) Soybean oligopeptides: Same as in Example 1.

[0076] (3) Ganoderma lucidum polysaccharide: pass through a 50-mesh sieve, with a material-to-liquid ratio of 1:25; ultrasonically extract at 400W and 75℃ for 2 hours; concentrate to 1:6, add 4 times the volume of 95% ethanol, and let stand at 6℃ for 14 hours.

[0077] (4) Sea buckthorn fruit oil: Extracted at 35MPa, 50℃, 25L / h for 2.5h.

[0078] (5) Total flavonoids from calendula: 80% ethanol was added at a material-to-liquid ratio of 1:20; microwave extraction was performed at 500W and 65℃ for 50 min.

[0079] (6) Mixing: Mix at 35 rpm, -0.07 MPa, and room temperature for 25 min. Other steps are the same as in Example 1.

[0080] Example 4 A cream for preventing or eliminating stretch marks, the components and preparation method of which are as follows: Active composition (Example 1): 20%; Oil phase excipients: cetearyl alcohol 3.5%, glyceryl monostearate 2.5%, caprylic / capric triglyceride 2.5%, vitamin E acetate 0.4%; Aqueous excipients: 5% glycerin, 1.5% panthenol, 0.15% sodium hyaluronate, 0.25% carbomer 940, 0.2% triethanolamine, 0.18% methylparaben, 0.4% phenoxyethanol; Purified water: Add to 100%.

[0081] Preparation method: Oil phase: Mix oil phase additives and dissolve and keep warm at 82℃.

[0082] Aqueous phase: Aqueous phase excipients (except triethanolamine and phenoxyethanol) are mixed with purified water and kept at 82℃.

[0083] Emulsification: The oil phase is slowly added to the water phase, sheared at 12000 rpm for 5 min, and then the temperature is gradually reduced in a vacuum emulsification pot (-0.07 MPa, 40 rpm): 82℃ → 62℃ (2℃ / min) for 10 min → 39℃ (1℃ / min) for 15 min.

[0084] Add active ingredient: Add the composition of Example 1 at 39°C and stir at 20 rpm for 15 min.

[0085] pH adjustment and discharge: Adjust the pH to 6.0 with triethanolamine, add phenoxyethanol and stir at 20 rpm for 5 min, then filter through a 200 mesh filter.

[0086] Example 5 A cream for preventing or eliminating stretch marks, with the following components: Active composition (Example 2): 15%; Oil phase excipients: 3% cetearyl alcohol, 2% glyceryl monostearate, 2% caprylic / capric triglyceride, 0.3% vitamin E acetate; Aqueous excipients: 4% glycerin, 1% panthenol, 0.1% sodium hyaluronate, 0.2% carbomer 940, 0.15% triethanolamine, 0.15% methylparaben, 0.3% phenoxyethanol; Purified water: Add to 100%.

[0087] The preparation method is the same as in Example 4.

[0088] Example 6 A cream for preventing or eliminating stretch marks, with the following components: Active composition (Example 3): 25%; Oil phase excipients: 4% cetearyl alcohol, 3% glyceryl monostearate, 3% caprylic / capric triglyceride, 0.5% vitamin E acetate; Aqueous excipients: 6% glycerin, 2% panthenol, 0.2% sodium hyaluronate, 0.3% carbomer 940, 0.25% triethanolamine, 0.2% methylparaben, 0.5% phenoxyethanol; Purified water: Add to 100%.

[0089] The preparation method is the same as in Example 4.

[0090] This invention conducted the following in vitro cell function experiments (Experiment 1) and human efficacy clinical trials (Experiment 2). All experiments included parallel controls, and the data were statistically analyzed using SPSS 26.0 software. P <0.05 indicates that the difference is statistically significant. P <0.01 indicates that the difference is statistically significant.

[0091] Experimental Example 1 1. Test materials 1.1 Cell line: Human dermal fibroblasts (HSF); 1.2 Main reagents: DMEM high glucose cell culture medium, fetal bovine serum, CCK-8 cell proliferation kit, human type I collagen ELISA kit, human type III collagen ELISA kit, human elastin ELISA kit, human MMP-9 ELISA kit; 1.3 Test Samples: Complete composition of Example 1 of the present invention, sample of discarded polypeptide group, sample of discarded plant extract group, and sample of discarded marula oil + argan oil group.

[0092] 2. Sample preparation and drug concentration All samples were diluted with DMEM culture medium containing 10% fetal bovine serum to a non-cytotoxic concentration of 0.1 mg / mL. This concentration was verified in preliminary experiments to have a cell viability of ≥90%, which is the optimal dosage concentration. Dosage: 100 μL per well of cells.

[0093] 3. Experimental Grouping (1) Blank control group: Normal cell culture, only an equal volume of DMEM culture medium was added, without any sample; (2) Complete formulation group: Add 0.1 mg / mL of the complete composition solution of Example 1 of the present invention; (3) Discarded polypeptide group: It does not contain frog skin elastic polypeptide and soybean oligopeptide. The remaining components are consistent with the complete formula and are prepared into a 0.1 mg / mL solution. (4) Extracts omitted: The extracts do not include Ganoderma lucidum polysaccharide extract, sea buckthorn fruit oil extract, or calendula total flavonoid extract. The remaining components are the same as the complete formula and are prepared into a 0.1 mg / mL solution. (5) Oil-free group: without marula oil and argan oil, the remaining components are the same as the complete formula, and are prepared into a 0.1 mg / mL solution.

[0094] 4. Detection Indicators Relative cell proliferation rate, type I collagen content, type III collagen content, elastin content, and MMP-9 inhibition rate.

[0095] 5. Test Methods HSF cells were loaded at 5 × 10 4 Cells were seeded at a density of 100 μL / mL in 96-well and 6-well culture plates and incubated at 37°C with 5% CO2 for 24 h. After adhesion, the corresponding sample solutions were added to each well according to the above groups, with a drug dosage of 100 μL / well. An equal volume of culture medium was added to the blank group, and the cells were cultured for another 48 h. The relative cell proliferation rate was detected by CCK-8 assay, and the content of type I collagen, type III collagen, elastin and MMP-9 inhibition rate in the cell supernatant were detected by ELISA. Each group was set up with 3 parallel replicates, and the experiment was repeated 3 times. The average value was used to calculate the results.

[0096] 6. Test Results As shown in Table 1, compared with the blank control group, the relative cell proliferation rate, type I collagen content, type III collagen content, elastin content, and MMP-9 inhibition rate of the complete formula group were all significantly increased. P <0.01); all indicators in the peptide group, extract group, and oil group were significantly higher than those in the blank control group ( P <0.05), but significantly lower than the complete formulation group ( P <0.01). The results show that the polypeptides, plant extracts, and natural oil components in the composition of the present invention work synergistically to significantly promote the proliferation of dermal fibroblasts in human skin, greatly enhance the synthesis capacity of collagen and elastin, and effectively inhibit the activity of matrix metalloproteinase-9, protecting skin fibers from degradation.

[0097] Table 1 Results of in vitro cell function tests of different compositions

[0098] Note: Compared with the blank control group, Compared with the complete formulation, # P <0.05, ## P <0.01.

[0099] Experimental Example 2 1. Experimental Design Randomized control, blank control, excipient matrix control, parallel control of commercially available products; self-before and after control; Test environment: temperature 22±2℃, humidity 50±5%. Subjects entered the test environment 30 minutes before the test to balance their skin condition.

[0100] 2. Subject Inclusion Criteria (1) Female aged 20-40, in good health, with no serious underlying diseases; (2) Noticeable stretch marks on the abdomen, thighs or buttocks: red stretch marks (the color is red, purplish red or dark red, slightly raised, without obvious depressions); white stretch marks (the color is silvery white or porcelain white, and they are in depressions). (3) I have not used any stretch mark repair skin care products or essential oils in the past 3 months, and I have not received any stretch mark fading medical aesthetic treatments (such as laser, microneedling, etc.). (4) No allergic constitution, and no history of allergy to the compound and matrix components of this invention (a skin patch test is required before the test). (5) Voluntarily participate in this test, sign the informed consent form, and strictly abide by the test requirements (apply the product on time, test on time, and do not use other interfering products).

[0101] 3. Exclusion criteria for participants (1) Allergic constitution, or a history of severe skin allergies, eczema, dermatitis or other skin diseases within the past year; (2) The skin in the stretch mark area is damaged, ulcerated, infected, or has scar hyperplasia; (3) Have taken oral retinoids within the past 3 months (affecting skin metabolism and interfering with test results); (4) Pregnant or breastfeeding women (to avoid potential effects of the product on the fetus / infant); (5) Having underlying diseases such as diabetes or immune system disorders that may affect the skin's ability to repair itself; (6) Those who fail to use the product as required by the test or complete the test on time, and who have poor compliance.

[0102] 4. Test Samples and Grouping 120 eligible participants with stretch marks were recruited and randomly divided into 4 groups of 30 each. There were no significant differences in age, type of stretch marks, or duration of the condition among the groups. P >0.05), indicating comparability: (1) Blank control group: No repair products were used, and the skin was only cleansed with water; (2) Excipient matrix group: a blank matrix paste containing only cream excipients and not containing the composition of the present invention; (3) Cream group of the present invention: the cream for preventing or eliminating stretch marks prepared in Example 4 of the present invention; (4) Commercially available products: Use mainstream commercially available stretch mark repair cream A.

[0103] 5. Administration method and dosage Frequency of use: Once in the morning and once in the evening, at fixed times; Dosage: Take 2g of sample each time, apply evenly to the stretch mark area of ​​the abdomen, and massage clockwise for 3-5 minutes until fully absorbed; Trial period: Use continuously for 8 weeks. Do not replace or use other wrinkle-reducing or repairing products during the trial period.

[0104] 6. Testing Instruments and Indicators Testing instruments: Cutometer MPA580 skin elasticity tester, PRIMOS 3D skin contour analyzer, CM-700d spectrophotometer; Testing indicators: (1) Total elasticity of skin (R2 value); (2) Reduction rate of stretch mark area; (3) Red stripes a Value decline rate; (4) The decrease in skin roughness Ra value.

[0105] 7. Test Results As shown in Table 2, after 8 weeks of continuous use, compared with the blank control group, the excipient matrix group showed significant improvements in skin elasticity, stretch mark reduction, and other indicators. P <0.05), all indicators of the cream group of the present invention were significantly improved ( P <0.01); compared with commercially available products, the cream group of the present invention showed improvements in total skin elasticity R2 value, reduction rate of stretch mark area, and reduction of erythema a. It was superior in terms of both the rate of decrease in skin roughness (Ra) and the decrease in skin roughness (Ra), and the difference was statistically significant. P <0.01). This confirms that the stretch mark prevention or elimination cream of the present invention is significantly more effective than similar commercially available products in improving skin elasticity, reducing the area of ​​stretch marks, fading redness, and improving skin roughness.

[0106] Table 2. Clinical trial results of human efficacy of different cream products.

[0107] Note: Compared with the blank control group, Compared with commercially available products, # P <0.05, ## P <0.01.

[0108] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A composition for preventing or eliminating stretch marks, characterized in that, The following raw materials are included by weight: The ingredients include 3-5 parts of forest frog skin elastic peptides, 2-4 parts of soybean oligopeptides, 2-3 parts of Ganoderma lucidum polysaccharide extract, 1.5-2.5 parts of sea buckthorn fruit oil extract, 1-2 parts of calendula total flavonoid extract, 4-6 parts of marula oil, and 3-5 parts of argan oil.

2. The composition according to claim 1, characterized in that, The molecular weight of the forest frog skin elastic polypeptide is 800~1500 Da; the molecular weight of the soybean oligopeptide is 500~1200 Da.

3. A method for preparing the composition according to claim 1 or 2, characterized in that, Includes the following steps: The following ingredients were prepared separately: forest frog skin elastic peptides, soybean oligopeptides, Ganoderma lucidum polysaccharide extracts, sea buckthorn fruit oil extracts, and calendula total flavonoid extracts. These were then mixed with marula oil and arugula oil in a specific ratio to obtain the composition.

4. The preparation method according to claim 3, characterized in that, The preparation steps of the frog skin elastic polypeptide are as follows: Raw material pretreatment: Clean and mince the frog skin, add purified water at a material-to-liquid ratio of 1:8 to 1:12, and homogenize; Compound enzymatic hydrolysis: Papain and neutral protease are added at an enzyme activity ratio of 1:1.5 to 1:2.

5. Enzymatic hydrolysis is carried out at 40 to 50°C and pH 6.5 to 7.5 for 3 to 5 hours, and enzyme inactivation is carried out at 85 to 95°C for 8 to 12 minutes. Membrane separation: The enzymatic hydrolysate is separated by a 1500 Da ultrafiltration membrane, the permeate is collected, and concentrated under reduced pressure at 50~60℃ and a vacuum of -0.07~-0.09 MPa; Drying: Spray drying to obtain the forest frog skin elastic polypeptide.

5. The preparation method according to claim 3, characterized in that, The preparation steps of the soybean oligopeptides are as follows: soybean protein is hydrolyzed by alkaline protease, separated by a 1200 Da membrane, and spray-dried to obtain soybean oligopeptides with a molecular weight of 500~1200 Da.

6. The preparation method according to claim 3, characterized in that, The preparation steps of the Ganoderma lucidum polysaccharide extract are as follows: Ganoderma lucidum fruiting bodies are pulverized and passed through a 30-50 mesh sieve, purified water is added at a material-to-liquid ratio of 1:15-1:25, and ultrasonic extraction is performed at 200-400W and 65-75℃ for 1-2 hours, followed by filtration; the filtrate is concentrated to 1:4-1:6, 2-4 times the volume of 90%-95% ethanol is added, and the mixture is allowed to stand at 2-6℃ for 10-14 hours, centrifuged to collect the precipitate, and then freeze-dried under vacuum. The preparation steps of the sea buckthorn fruit oil extract are as follows: supercritical CO2 extraction, extraction pressure 25~35MPa, temperature 40~50℃, CO2 flow rate 15~25L / h, extraction for 1.5~2.5h; The preparation steps of the total flavonoid extract of calendula are as follows: calendula flowers are crushed, 60% to 80% ethanol is added at a material-to-liquid ratio of 1:10 to 1:20, microwave extraction is performed at 300 to 500W and 55 to 65℃ for 30 to 50 minutes, followed by filtration, concentration under reduced pressure, and vacuum drying.

7. The use of the composition of claim 1 or 2 in the preparation of a cream product for preventing or eliminating stretch marks.

8. A cream for preventing or eliminating stretch marks, characterized in that, It includes 15-25 wt% of the composition according to claim 1 or 2, excipients, and purified water.

9. The cream according to claim 8, characterized in that, The excipients include oil-phase excipients and aqueous-phase excipients; The oil phase excipients consist of 3-4 wt% cetearyl alcohol, 2-3 wt% glyceryl monostearate, 2-3 wt% caprylic / capric triglyceride, and 0.3-0.5 wt% vitamin E acetate. The aqueous phase excipients are 4-6 wt% glycerol, 1-2 wt% panthenol, 0.1-0.2 wt% sodium hyaluronate, 0.2-0.3 wt% carbomer 940, 0.15-0.25 wt% triethanolamine, 0.15-0.2 wt% methylparaben, and 0.3-0.5 wt% phenoxyethanol.

10. A method for preparing the cream according to claim 8 or 9, characterized in that, Includes the following steps: Oil phase preparation: Mix the oil phase additives, heat to 80~85℃ to dissolve, and keep warm; Aqueous phase preparation: Glycerin, panthenol, sodium hyaluronate, carbomer, and methylparaben are added to purified water, dispersed evenly, heated to 80-85℃, and kept at that temperature; Emulsification: The oil phase is slowly added to the water phase, shear emulsification is performed, and the mixture is transferred to a vacuum emulsification pot for gradient cooling; the gradient cooling is 80~85℃→60~65℃, held for 10min→38~40℃, held for 15min. Add active ingredients: Add the composition at 38~40℃ and stir; pH adjustment and discharge: Adjust the pH to 5.5~6.5 with triethanolamine, add phenoxyethanol, stir, and filter to discharge.

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