Preparation method and application of enzyme control nutrition microspheres with built-in glucose transfer system

By preparing enzyme-controlled nutrient microspheres with built-in glucose delivery systems, the problems of uncontrollable glucose release and osmotic pressure imbalance have been solved, achieving safe energy supply and improved cell survival rate, and has broad application prospects in tissue regeneration.

CN122398735APending Publication Date: 2026-07-17QILU INST OF TECH
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Patent Information

Application Number
CN202610558895.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-04-25
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing technologies suffer from problems such as uncontrollable glucose release, easy osmotic pressure imbalance, inactivation of exogenous enzymes inside microspheres, performance limitations of natural polysaccharides and synthetic polymers, residual organic solvents, and high costs.

Method used

Enzyme-controlled nutrient microspheres with an integrated glucose delivery system were prepared by dissolving a hydrogel carrier, glucosyl amylase (AMG), and a cross-linking agent in an aqueous starch solution to form an aqueous phase. The aqueous phase was then mixed with an emulsifier and solvent, heated and stirred, cross-linked under ultraviolet light, and freeze-dried. Starch molecules formed a low-osmotic-pressure active reservoir in the GelMA network, and glucose was slowly diffused after enzymatic hydrolysis.

Benefits of technology

It achieves safe energy supply, reduces the risk of osmotic pressure imbalance, improves the performance limitations of natural polysaccharides and synthetic polymers, provides controllable degradation and cell adhesion, and improves cell survival rate and the stability of glucose release.

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Abstract

The invention relates to a preparation method and application of an enzyme control nutrition microsphere with a built-in glucose transfer system, and belongs to the technical field of nutrition microspheres. The preparation method comprises the following steps: dissolving the hydrogel carrier, the glucoamylase AMG and the cross-linking agent in a starch aqueous solution to obtain a water phase; mixing an emulsifier and a solvent to obtain an oil phase; dropwise adding the water phase into the oil phase, heating, stirring and mixing, placing under the irradiation of an ultraviolet light source, carrying out cross-linking reaction, removing supernate through high-speed centrifugation, taking a solid phase, placing the obtained solid phase into a refrigerator at the temperature of 80 DEG C below zero, and pre-cooling for more than 24 hours; and freeze-drying to obtain the enzyme control nutrition microsphere with the built-in glucose transfer system. The nutritional microspheres provided by the invention can reduce the risk of osmotic pressure imbalance caused by high-concentration glucose and improve the performance limitation of natural polysaccharides and synthetic polymers.
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