Reagent compositions and methods of preparing lyophilized reagents
By using a combination of rabbit plasma, trehalose, and PBS buffer, along with liquid nitrogen droplet freeze-drying technology, the problems of inaccurate results and poor stability of freeze-dried products caused by improper selection of plasma source in coagulase assays were solved, achieving high formation rate, good resolubility, and preservation of coagulase activity in freeze-dried products.
Patent Information
- Application Number
- CN202610845794.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-06-12
- Publication Date
- 2026-07-17
AI Technical Summary
In existing technologies, improper selection of plasma sources leads to inaccurate coagulase test results, poor stability, and problems such as appearance collapse, low molding rate, poor resolubility, and severe loss of coagulase activity in freeze-dried products during the freeze-drying process.
A combination of rabbit plasma, trehalose, and PBS buffer was used to form a regular lyophilized product through mixing, sterile filtration, and liquid nitrogen droplet lyophilization under light-protected conditions. Trehalose replaced water molecules to form hydrogen bonds with plasma proteins, mannitol formed a crystal framework, and PBS buffer maintained pH stability.
It improves the sensitivity and stability of coagulase assays, increases the formation rate and resolubility of lyophilized products, reduces the loss of coagulase activity, and extends shelf life.
Smart Images
Figure CN122405792A_ABST