Determination method for sn-2 site fatty acid distribution in grease rich in long-chain polyunsaturated fatty acid

By employing a method of full-process antioxidant protection and quality control sample calibration, the problems of acyl migration and oxidative loss were solved, achieving accuracy and reproducibility of the distribution of fatty acids at the sn-2 position in oils rich in long-chain polyunsaturated fatty acids. This method is suitable for nutritional evaluation of products such as infant formula and specific medical fat emulsions.

CN122409883APending Publication Date: 2026-07-17瞿瀚鹏
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Patent Information

Application Number
CN202610324797.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-03-17
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing technologies for determining the distribution of fatty acids at the sn-2 position in oils rich in long-chain polyunsaturated fatty acids suffer from problems such as acyl migration, leading to poor detection accuracy and reproducibility. Furthermore, the easy oxidation of long-chain polyunsaturated fatty acids results in inaccurate detection results.

Method used

The process was calibrated using full-process antioxidant protection measures and quality control samples. Oils were hydrolyzed by specific lipases at the sn-1 and 3 positions, and the proportion of fatty acids at the sn-2 position was calculated by combining thin-layer chromatography and gas chromatography. The hydrolysis efficiency and separation recovery rate were corrected using quality control oil samples.

Benefits of technology

It effectively avoids the oxidative loss of long-chain polyunsaturated fatty acids, improves the accuracy and reproducibility of detection data, and is suitable for the analysis of the distribution of fatty acids at the sn-2 position in complex oils.

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Abstract

The invention discloses a method for determining sn-2 site fatty acid distribution in grease rich in long-chain polyunsaturated fatty acid, and belongs to the field of analytical chemistry and food science. According to the method, a quality control grease sample is introduced, the quality control grease sample contains correction fatty acid, and the correction fatty acid is combined to any acyl position of a triglyceride molecule; a correction factor is obtained through calculation according to actual measurement data of the quality control grease sample, and the correction factor is used for correcting enzymolysis efficiency and analyzing system deviation. According to the method, the quality control sample is innovatively introduced to carry out process monitoring and correction on the enzymolysis efficiency and the separation recovery rate, so that system errors caused by factors such as enzyme activity fluctuation and operation difference are eliminated, and the reproducibility of the method among different operators at different time is greatly improved.
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