VMHvl brain region positioning method suitable for animal with skull seam variation

By using the posterior fontanelle as a reference in long-clawed gerbils, and employing a digital stereotaxic instrument and Z-axis leveling technology, the problem of positioning errors caused by cranial suture variations was solved, achieving high-precision positioning of the VMHvl nucleus, which is suitable for neurological function experiments.

CN122440337APending Publication Date: 2026-07-24HUZHOU UNIVERSITY
0 Cites 0 Cited by

Patent Information

Application Number
CN202610286331.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-03-10
Publication Date
2026-07-24

AI Technical Summary

Technical Problem

Existing stereotaxic techniques for brain localization are limited by the absence of visible landmarks or the need for subjective judgment in animals with cranial suture variations. This results in large localization errors in the VMHvl nucleus and fails to meet the high-precision localization requirements of outbred animals such as the long-clawed gerbil.

Method used

Using a digital stereotactic instrument with the posterior fontanelle as a reference, the anterior fontanelle is determined by the intersection of the sagittal suture and the herringbone suture. Combined with the Z-axis height difference for quantitative leveling, the insertion point of the VMHvl is established, and a hole is drilled to expose the dura mater, thus achieving precise insertion of the positioning needle.

Benefits of technology

It significantly improves the accuracy and consistency of VMHvl nucleus localization, with needle path offset ≤5μm and nucleus overlap rate ≥95%, meeting the requirements of neurofunctional experiments and reducing functional phenotype error.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The invention discloses a VMHvl brain region positioning method suitable for a cranial seam variation animal, and aims to solve the problems of large system error (ML offset of 155.31 + / -25.57 microns) and target failure of a traditional fontanet positioning method caused by fuzzy intersection point and bifurcation of sagittal seams and coronary seams of long-claw gerbil and other far-crossing animals. According to the method, the intersection point of a sagittal seam and a herringbone seam serves as a rear chimney, and after a digital display positioning instrument is reset, the digital display positioning instrument moves backwards to the rear chimney from the front chimney pre-estimation position along the Y axis; the positioning needle is moved forwards from the rear chimney at a constant speed, and the visual intersection point of the positioning needle and the coronary seam is the front chimney; then front-back leveling is achieved through the fact that the front-back chimney Z value difference is smaller than or equal to 0.03 mm, and left-right leveling is achieved through the fact that the left-right two-point Z value difference is smaller than or equal to 0.05 mm; and finally, the VMHvl is positioned according to ML + / -0.75 mm, AP-1.7 mm and DV-7.5 mm by taking a fontangle as an original point. According to the invention, ML offset is reduced to 2.29 + / -0.96 [mu] m (P is less than 0.001), the target qualification rate is 100%, the coincidence rate is greater than or equal to 95%, and the reliability of downstream experiments such as optogenetics and electrophysiology is obviously improved.
Need to check novelty before this filing date? Find Prior Art