Application of CmANL2 protein and coding gene thereof in regulation and control of anthocyanin accumulation or chrysanthemum flower color

By overexpressing or inhibiting the CmANL2 protein and its gene in chrysanthemum, and utilizing blue light regulation technology, the application problem of blue light signal in anthocyanin accumulation was solved, achieving low-energy consumption and high-quality flower color enhancement, and promoting the accumulation of anthocyanins in chrysanthemum.

CN122444845APending Publication Date: 2026-07-24SANYA INSTITUTE OF NANJING AGRICULTURAL UNIVERSITY
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Patent Information

Application Number
CN202610904278.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-06-23
Publication Date
2026-07-24

AI Technical Summary

Technical Problem

Existing technologies have failed to effectively utilize blue light signals to regulate the accumulation of anthocyanins in chrysanthemums, making it difficult to improve flower color quality, especially in facility horticulture production where there is a problem of high energy consumption.

Method used

By overexpressing or inhibiting the CmANL2 protein and its encoding gene in chrysanthemum, and utilizing Agrobacterium-mediated leaf disc transformation and blue light irradiation, the accumulation of anthocyanins was regulated, thereby improving flower color quality.

Benefits of technology

Under low energy consumption conditions, it significantly promotes the accumulation of anthocyanins in chrysanthemum and improves flower color stability, providing theoretical guidance for molecular breeding of new chrysanthemum germplasm with high anthocyanin accumulation.

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Abstract

The invention discloses an application of CmANL2 protein and a coding gene thereof in regulation and control of anthocyanin accumulation or chrysanthemum flower color. The amino acid sequence of the CmANL2 protein is as shown in SEQ ID NO: 3, and the genome nucleotide sequence and the CDS sequence of the coding gene of the CmANL2 protein are as shown in SEQ ID NO: 1 and SEQ ID NO: 2 respectively. Experimental results show that activation of expression of the CmANL2 gene can significantly promote the process of anthocyanin accumulation in chrysanthemum petals and maintain flower color stability; inhibition of expression of the CmANL2 gene can aggravate the color fading degree of the chrysanthemum, so that the flower color becomes light. The invention provides a key target for a new variety of chrysanthemum with stable flower color in response to blue light induction, and can be applied to breeding, cultivation and popularization of ornamental chrysanthemum.
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