Recombinant bacterium for producing ammonium acetate and method for preparing ammonium acetate
By constructing a recombinant Escherichia coli strain and optimizing the fermentation process, the deficiencies of raw materials and processes in the existing ammonium acetate production were solved, achieving efficient, economical, and green ammonium acetate production, and reaching the goals of high yield and high purity.
Patent Information
- Application Number
- CN202610884022.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-06-18
- Publication Date
- 2026-07-24
AI Technical Summary
Existing ammonium acetate production processes suffer from drawbacks such as non-renewable raw materials, harsh reaction conditions, high energy consumption, large emissions of waste, and products that are prone to containing inorganic impurities. These drawbacks make it difficult to meet the requirements of high-end fields for high-purity, low-residue, and environmentally friendly products. Furthermore, the two-step fermentation process is lengthy, requires significant equipment investment, is complex to operate, has high costs, and involves cumbersome purification steps, making it difficult to achieve efficient, economical, and green large-scale preparation of ammonium acetate.
By constructing a recombinant Escherichia coli strain, knocking out key subunits of pyruvate oxidase, pyruvate formate lyase, and pyruvate dehydrogenase, and overexpressing genes such as glyoxylate transaminase and hydroxyaspartate aldolase, an acetyl-CoA carbon sequestration pathway was established. Fermentation process parameters, such as integrated pH and CO2 regulation, staged dissolved oxygen control, and fed-batch sugar supplementation, were optimized to achieve the direct conversion of glucose into ammonium acetate.
It has achieved efficient, economical and green ammonium acetate production. Single-strain single-step fermentation replaces two fermentation systems, which improves carbon utilization, simplifies process control, reduces impurities, and improves product purity and yield, achieving an ammonium acetate yield of 70 g/L and a yield of 95%.