Transaminase for degrading and hydrolyzing fumonisins HFB1 and application of transaminase

By developing a novel transaminase, FumSPTA, the problem of scarce HFB1 degrading enzyme resources has been solved, enabling efficient and stable conversion of HFB1 into low-toxic products suitable for agriculture, feed, and food industries, thus reducing the health risks of fumonisins.

CN122445604APending Publication Date: 2026-07-24SOUTH CHINA AGRICULTURAL UNIVERSITY
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Patent Information

Application Number
CN202610676797.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-05-18
Publication Date
2026-07-24

AI Technical Summary

Technical Problem

The existing HFB1 degrading enzyme resources are scarce and have insufficient catalytic activity, making it difficult to achieve efficient and stable detoxification, and posing safety hazards and the risk of nutrient loss.

Method used

A novel transaminase, FumSPTA, was developed to achieve efficient soluble expression via a prokaryotic expression system. When combined with the coenzyme pyridoxal phosphate (PLP), it maintains high catalytic activity over a wide temperature and pH range and can convert HFB1 into the low-toxicity product 2-keto-HFB1.

Benefits of technology

Under optimal conditions, FumSPTA achieves a 70% degradation rate in 1 minute when 1 μg of enzyme reacts with 1 μg of HFB1, and complete degradation is achieved within 30 minutes. It has high degradation efficiency, wide applicability, and reduces the toxicity of HFB1.

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Abstract

The invention discloses transaminase FumSPTA for degrading and hydrolyzing fumonisins HFB1 and application of the transaminase FumSPTA, and belongs to the technical field of agricultural biology. The transaminase is derived from sphingosine box bacteria, and the amino acid sequence of the transaminase is as shown in SEQ ID NO. 1. The enzyme can realize efficient soluble expression through a prokaryotic expression system, and keeps high degradation activity in a wide temperature range of 30-70 DEG C and in a pH range of 7-10. Under the optimum condition, the degradation rate can reach 70% after 1 microgram of enzyme reacts with 1 microgram of HFB1 for 1 minute, complete degradation can be achieved within 30 minutes, and the degradation product is low-toxicity 2-keto-HFB1. By pre-combining the coenzyme pyridoxal phosphate, the enzyme can keep high catalytic activity without additionally adding coenzyme in the subsequent application stage, so that the application process is remarkably simplified, and the use cost is reduced. Compared with the HFB1 degrading enzyme reported at present, the FumSPTA disclosed by the invention has remarkable advantages in the aspects of expression efficiency and catalytic activity. The fumonisins enzyme can be applied to industries such as agriculture, feed and food, and the harm of fumonisins to the health of animals and human beings is reduced.
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