Breeding method for breeding new silkworm variety containing human bone morphogenetic protein in natural silk

By constructing a specific expression vector and the piggyBac transposon system in silkworms, and combining multiple generations of backcrossing and self-crossing verification, the problem of stable expression and maintenance of economical traits of hBMP2 protein was solved, achieving low-cost, high-efficiency production and high-value-added applications.

CN122445657APending Publication Date: 2026-07-24SANTAI SILKWORM FARM IN SICHUAN PROVINCE +2
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Patent Information

Application Number
CN202610418572.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-04-01
Publication Date
2026-07-24

AI Technical Summary

Technical Problem

Existing technologies make it difficult to stably express human bone morphogenetic protein 2 (hBMP2) in silkworms and maintain their economic traits, resulting in high production costs, poor safety, and the inability of traditional breeding methods to introduce exogenous genes.

Method used

A specific expression vector was constructed to drive the expression of the hBMP2 gene using the silk fibroin heavy chain promoter. The gene was then stably integrated into the silkworm genome using the piggyBac transposon system. After multiple generations of backcrossing and self-crossing verification, the stable inheritance of the exogenous gene and the maintenance of economic traits were ensured.

Benefits of technology

This study achieved efficient secretory expression and low-cost production of hBMP2 protein, maintained the economic traits of silkworms, expanded the functional applications of silkworm cocoons, and provided high-value-added biomedical materials.

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Abstract

The invention belongs to the technical field of silkworm genetic breeding and biology, and particularly relates to a breeding method for breeding a new silkworm variety containing human bone morphogenetic protein in natural silk. The method comprises the following steps: constructing a proper vector, and introducing a human-derived hBMP2 gene which does not exist in a silkworm genome originally; by means of genetic breeding, it is ensured that the exogenous gene is stably inherited and not lost in multi-generation breeding, and meanwhile excellent economic characters and healthy characters can be ensured; the silk containing the recombinant human bone morphogenetic protein hBMP2 is obtained, large-scale and planned production of the hBMP2 protein is achieved through an existing silkworm cocoon production mode, the requirement of the market for the hBMP2 protein is met, the application limitation of traditional silkworm cocoon reeling silk weaving silk is broken through, and the silkworm cocoon production economic efficiency is improved.
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