Method for analyzing methyl mercury methyl carbon in complex matrix

By combining phenyl derivatization and solid-phase microextraction with gas chromatography-mass spectrometry, the problem of trace analysis of methyl carbon in methylmercury in complex microbial culture systems has been solved, achieving high-sensitivity and high-stability detection and isotope characterization.

CN122449005APending Publication Date: 2026-07-24HANGZHOU INST FOR ADVANCED STUDY UCAS
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Patent Information

Application Number
CN202610545962.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-04-23
Publication Date
2026-07-24

AI Technical Summary

Technical Problem

Existing technologies struggle to achieve stable detection and isotopic characterization of trace methylmercury methyl carbon in complex microbial culture systems. They suffer from problems such as low target analyte content, complex culture medium composition, strong matrix interference, limited enrichment efficiency during pretreatment, and insufficient detection response.

Method used

A phenylation derivatization strategy was employed to convert methylmercury into phenylated derivatives, which were then analyzed using headspace solid-phase microextraction and gas chromatography-mass spectrometry. The derivatization reaction conditions, extraction temperature and time, as well as the inlet temperature and carrier gas flow rate, were optimized to improve the volatility and detection response of the target analyte.

Benefits of technology

This method improves the enrichment efficiency and detection sensitivity of methylmercury methyl carbon in complex culture media, reduces matrix interference, and enables stable analysis and isotopic characterization of trace methylmercury, making it suitable for studying the source of methyl carbon in microbial culture systems.

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Abstract

The invention discloses a method for analyzing methyl mercury methyl carbon in a complex matrix, which comprises the following steps: taking a to-be-detected sample containing methyl mercury, and adding sodium tetraphenylborate to carry out phenylation derivation reaction; carrying out headspace solid-phase microextraction enrichment on the derivatization sample; introducing the enriched solid-phase microextraction fibers into a gas chromatograph-mass spectrometer for thermal desorption, separation and detection; and performing qualitative or quantitative analysis on methyl mercury methyl carbon according to retention time, mass spectrum response signals and isotope characteristics. Aiming at the problems of low content of methyl mercury and strong matrix interference in complex matrixes such as a microbial culture system and the like, the enrichment efficiency and the detection sensitivity are improved and the matrix interference is reduced by combining phenylation derivation, solid-phase microextraction enrichment and gas chromatography-mass spectrometry detection, and the method has the characteristics of high sensitivity, good matrix adaptability and stable repeatability and is suitable for popularization and application. The method is suitable for analysis of methyl carbon of trace methyl mercury in a complex culture medium and isotope characteristic determination of a C labeling system.
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