A colloidal gold immunochromatographic test strip for detecting antibodies of multiple species of toxoplasma, a preparation method and application thereof

By employing a dual-antigen gold label pad and a DNP independent quality control system, the high dependence of SAG1 antibody and the challenges of multi-species detection in existing technologies have been solved, enabling rapid and accurate detection of Toxoplasma gondii antibodies in multiple species, which is suitable for farms and clinical applications.

CN122449121APending Publication Date: 2026-07-24HENAN ACAD OF AGRI SCI +1
View PDF 2 Cites 0 Cited by

Patent Information

Application Number
CN202610783174.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-06-02
Publication Date
2026-07-24

AI Technical Summary

Technical Problem

Existing Toxoplasma gondii antibody detection technologies suffer from high costs and long preparation cycles due to the reliance on SAG1 antibody recognition of natural conformations for quality control lines, and cannot achieve multi-species detection. Furthermore, existing colloidal gold test strips have limited versatility.

Method used

Employing a dual-antigen gold-labeled pad and an independent DNP quality control system, this method uses colloidal gold-labeled SAG1 antigen and DNP-BSA conjugate, combined with Staphylococcus aureus protein A and anti-DNP antibody, to achieve multi-species detection, simplifying the process and reducing costs.

Benefits of technology

It enables rapid and accurate detection of Toxoplasma gondii antibodies across multiple species, reduces costs, improves quality control stability, is suitable for farm and clinical testing, and offers high sensitivity and reliable results.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN122449121A_ABST
    Figure CN122449121A_ABST
Patent Text Reader

Abstract

The present application belongs to the field of immune detection technology, and particularly relates to a colloidal gold immunochromatographic test strip for detecting antibodies of multiple species of Toxoplasma gondii, a preparation method and application thereof. The test strip uses Staphylococcus aureus protein A as a detection line, and can detect serum antibodies in various animals such as pigs, sheep, goats and cats. A DNP system is used as an independent quality control line, avoiding the dependence of a traditional quality control line on the spatial conformation of SAG1 protein, and improving the stability and repeatability of the test strip. The test strip is used in combination with a sample diluent to detect negative samples, and the negative coincidence rate reaches 100%. The test strip is used in combination with a sample diluent to detect clinical serum samples, and the coincidence rate with IFA reaches 97.6-100%, indicating that the test strip and the sample diluent have good diagnostic reliability, specificity and accuracy, are portable and simple to operate, and can determine the result by naked eye within 5 minutes without relying on professional equipment, and are suitable for on-site rapid detection and clinical detection in farms.
Need to check novelty before this filing date? Find Prior Art

Citation Information

Patent Citations

  • Detection kit for detecting toxoplasma gondii IgA

    CN117630362A

  • Colloidal gold immunochromatography test strip for detecting toxoplasma gondii antibody and application of colloidal gold immunochromatography test strip

    CN118425530A