Method for constructing an animal model of vitiligo by sequential combination of hydroquinone and monobenzone

Abstract

The present application relates to the technical field of vitiligo animal model construction, and discloses a method for constructing a vitiligo animal model by sequentially using hydroquinone and monobenzone, comprising the following steps: S1, animal preparation: taking 6-8-week-old mice, and shaving the hair in a 2cm*3cm area on the back; S2, oxidative stress pre-activation stage: daily applying a hydroquinone preparation to the pre-treated skin; S3, function-oriented transition period: stopping the use of hydroquinone for 1-3 days, and only applying a blank matrix to the skin; S4, immune cascade amplification stage: daily applying a monobenzone preparation to the same skin area. The present application first integrates the oxidative stress mechanism of HQ and the hapten immune mechanism of MBEH in time sequence, realizes a synergistic depigmentation effect, reduces the use concentration of HQ and MBEH through a sequential strategy, significantly reduces non-specific damage to the skin, and establishes a multi-dimensional evaluation system covering depigmentation area, histopathology, immunohistochemistry, serological indicators and transcriptomics.