Continuous flow fermentation device for recombinant protein production

Abstract

The utility model belongs to the technical field of protein production, and particularly relates to a continuous flow fermentation device for recombinant protein production, which comprises a reaction kettle body, the inner side wall of the reaction kettle body is fixedly connected with a pair of mounting blocks; the mounting blocks are symmetrically arranged on two sides of the reaction kettle body and have the same structure; the fixing blocks are inserted into the side walls of the inserting holes, elastic plates can automatically reset and abut against the side walls of the mounting blocks through elasticity, then the fixing frames can be fixed, and at the moment, protein attached to the inner side wall of the reaction kettle body can be scraped off through attachment of first scraping plates and the inner side wall of the reaction kettle body; according to the reaction kettle, protein attached to the inner side wall of the reaction kettle body can be cleaned through attached rotation of a first scraping plate and the inner side wall of the reaction kettle body, the convenience of cleaning the attached protein is improved, meanwhile, a fixing block is inserted into an insertion hole for fixing, the convenience of dismounting and maintaining the first scraping plate can be achieved, and the service life of the first scraping plate is prolonged. And therefore, the flexibility of the first scraper blade during use is improved.

Description

TECHNICAL FIELD

[0001] The utility model belongs to protein production technical field, specifically speak to the continuous flow type fermentation device for recombinant protein production. BACKGROUND

[0002] Protein is the material basis of life, is the basic organic matter of cell, plays the vital role in the life activities of organism.

[0003] In the prior art, fermentation device needs to be used in the production process of recombinant protein, and the protein is fully mixed and fermented through the continuous flow type fermentation device, and the fermentation device is mainly composed of a reaction kettle body and a stirring mechanism, in the use process, the protein and various raw materials are injected into the kettle body, and then the stirring mechanism is used to fully stir and mix them, so that the protein can be mixed with various raw materials for fully mixed fermentation.

[0004] However, in the prior art, it is found that the protein after fermentation has certain viscosity, so that part of the protein will adhere to the inner wall of the reaction kettle when being discharged, causing difficult cleaning, thereby increasing the workload, therefore, the continuous flow type fermentation device for recombinant protein production is proposed for the above problems. UTILITY MODEL CONTENT

[0005] In order to make up for the deficiencies of the prior art and solve at least one technical problem proposed in the background art, the utility model provides a continuous flow type fermentation device for recombinant protein production.

[0006] The utility model solves the technical problems and adopts the technical scheme: the utility model discloses a continuous flow type fermentation device for recombinant protein production, including reaction kettle body, the reaction kettle body inboard wall is fixedly connected with a pair of mounting blocks, the mounting block is symmetrically arranged on the both sides of reaction kettle body and the same structure, the mounting block lateral wall is provided with the insertion hole, the insertion hole lateral wall is connected with the fixed block of sliding, the fixed block lateral wall is fixedly connected with a pair of elastic plates, the fixed block lateral wall is fixedly connected with the fixed frame, the fixed frame lateral wall is fixedly connected with a pair of spring rods, the spring rod is symmetrically arranged on the both sides of fixed frame and the same structure, the spring rod end is fixedly connected with the same first scraper, can play the cleaning effect of the protein adhered to the reaction kettle body inboard wall, improve the convenience when cleaning the adhered protein, at the same time, the fixed block is inserted into the insertion hole and is fixed, can play the convenience when the first scraper is disassembled and maintained.

[0007] Preferably, the fixed block side wall is provided with a groove; the spring is fixedly connected to the groove side wall; the pressing rod is fixedly connected to the spring end; the connecting rope is fixedly connected between the pressing rod and the elastic plate; the connecting rope penetrates through the fixed block wall body and is in sliding connection with the same; the convenience of pressing the elastic plate can be achieved, and the convenience of disassembling the fixed block is improved.

[0008] Preferably, the first scraper side wall is fixedly connected with an elastic sheet; a plurality of elastic rods are fixedly connected to the reaction kettle body inner side wall; the elastic rods are uniformly distributed on the reaction kettle body inner side wall and are the same in structure; the elastic rods are fixedly connected with counterweight balls at the ends; the cleaning effect of the protein attached to the first scraper wall body can be achieved, the protein attachment is reduced, and the subsequent scraping effect of the first scraper is increased.

[0009] Preferably, the mounting block side wall is fixedly connected with a pair of limiting blocks; the limiting blocks are symmetrically arranged on both sides of the mounting block and are the same in structure; a plurality of grooves are formed in the limiting block side wall; the grooves are uniformly distributed on the limiting block side wall and are the same in structure; the grooves are rotatably connected with rollers; the limiting effect of inserting the fixed block into the side wall of the insertion hole can be achieved, and the convenience of inserting the fixed block is improved.

[0010] Preferably, a pair of supports are fixedly connected to the reaction kettle body inner side wall; the supports are symmetrically arranged on both sides of the reaction kettle body and are the same in structure; the supports are fixedly connected with second scrapers at the ends; the cleaning effect of the protein attached to the bottom of the reaction kettle body can be achieved, and the cleaning effect in the reaction kettle body is improved.

[0011] Preferably, the counterweight ball side wall is fixedly connected with a rubber sleeve; the protection effect of the elastic sheet when impacted can be achieved, and the damage caused by the impact of the counterweight ball on the elastic sheet is reduced.

[0012] The beneficial effects of the utility model are as follows:

[0013] The utility model provides a continuous flow type fermentation device for recombinant protein production, through the first scraper, through the fit rotation of the first scraper and the reaction kettle body inner side wall, the cleaning effect of the protein attached to the reaction kettle body inner side wall can be achieved, the convenience of cleaning the attached protein is improved, the first scraper can be fixed by inserting the fixed block into the insertion hole, the convenience of disassembling and maintaining the first scraper can be achieved, and the flexibility of the first scraper in use is improved.

[0014] The utility model provides a continuous flow type fermentation device for recombinant protein production, through the pressing rod, through the pressing of the pressing rod and the movement of the elastic plate, the convenience of pressing the elastic plate can be achieved, the convenience of disassembling the fixed block is improved, the pressing of the elastic plate on both sides is not convenient, and the workload is increased. DRAWINGS

[0015] The accompanying drawings, which are included to provide a further understanding of the present application and are incorporated in and constitute a part of this application, illustrate embodiments of the present application and together with the description serve to explain the present application. In the drawings:

[0016] Figure 1 is a perspective view of the present application;

[0017] Figure 2 is a perspective view of the second scraper in the present application;

[0018] Figure 3 is a perspective view of the fixed frame in the present application;

[0019] Figure 4 is Figure 3 is a local enlarged view of A in the present application;

[0020] Figure 5 is a perspective view of the first scraper in the present application;

[0021] Figure 6 is Figure 5 is a local enlarged view of B in the present application;

[0022] Figure 7 is a perspective view of the limiting block in the present application.

[0023] Legend:

[0024] 1, reaction kettle body; 11, mounting block; 12, jack; 13, fixed block; 14, elastic plate; 15, fixed frame; 16, spring rod; 17, first scraper; 2, groove; 21, spring; 22, pressing rod; 23, connecting rope; 3, elastic sheet; 31, elastic rod; 32, counterweight ball; 4, limiting block; 41, slot; 42, roller; 5, support; 51, second scraper; 6, rubber sleeve. DETAILED DESCRIPTION

[0025] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0026] Specific embodiments are given below.

[0027] Please refer to Figures 1-7The utility model provides a continuous flow type fermentation device for recombination protein production, including the reaction kettle body 1, the reaction kettle body 1 inside wall is fixedly connected with a pair of mounting block 11, the mounting block 11 is symmetrically arranged in the both sides of reaction kettle body 1 and same structure, the mounting block 11 lateral wall is set up with the jack 12, the jack 12 lateral wall is connected with the fixed block 13 of sliding, the fixed block 13 lateral wall is fixedly connected with a pair of elastic plate 14, the fixed block 13 lateral wall is fixedly connected with the fixed support 15, the fixed support 15 lateral wall is fixedly connected with a pair of spring rod 16, the spring rod 16 is symmetrically arranged in the both sides of fixed support 15 and same structure, the spring rod 16 end is fixedly connected with same first scraper 17, when using, by inserting the fixed block 13 into the jack 12 lateral wall, makes elastic plate 14 receive extrusion and the fixed block 13 lateral wall adhesion, when elastic plate 14 passes through the jack 12, utilizes the elasticity of elastic plate 14 and resets automatically and touches on the mounting block 11 lateral wall, can fix the fixed support 15, then through the stirring mechanism rotation stirring mix in the reaction kettle body 1 is completed, and the protein is discharged, then start stirring mechanism again, can make first scraper 17 produce rotation, when through the adhesion of first scraper 17 and the reaction kettle body 1 inside wall, can scrape the protein adhered on the reaction kettle body 1 inside wall, makes it fall to the bottom in the reaction kettle body 1 finally and is discharged to the outside, along with the full adhesion scraping of first scraper 17 and the reaction kettle body 1 inside wall, can cause the abrasion of first scraper 17, when then through the elasticity of spring rod 16 will push first scraper 17, make it always with the full adhesion of reaction kettle body 1 inside wall, to adhere to the protein on the reaction kettle body 1 inside wall cleaning can be completed, in the process, through the adhesion rotation of first scraper 17 and the reaction kettle body 1 inside wall, can play the cleaning effect of the protein adhered on the reaction kettle body 1 inside wall, improve the convenience of adhered protein cleaning, simultaneously, by inserting the fixed block 13 into the jack 12 and fixing, can play the convenience of first scraper 17 disassembly maintenance, further improve the flexibility of first scraper 17 when using.

[0028] Further, as Figure 6As shown, the side wall of the fixing block 13 has a groove 2; a spring 21 is fixedly connected to the side wall of the groove 2; a pressing rod 22 is fixedly connected to the end of the spring 21; a connecting rope 23 is fixedly connected between the pressing rod 22 and the elastic plate 14; the connecting rope 23 passes through the wall of the fixing block 13 and is slidably connected to it; in use, by pressing the pressing rod 22, it moves into the groove 2. At this time, the pressing rod 22 will squeeze the spring 21, and at the same time drive the connecting ropes 23 on both sides to move, pulling the elastic plate 14. Then the fixing block 13 can be removed from the side wall of the insertion hole 12. Finally, the pressing rod 22 is released, and the elasticity of the spring 21 can push the pressing rod 22 to automatically reset. In this process, the pressing rod 22 moves the elastic plate 14, which can facilitate the pressing of the elastic plate 14 and improve the convenience of disassembling the fixing block 13, reducing the inconvenience of pressing the elastic plates 14 on both sides and reducing the workload caused by the inconvenience of pressing the elastic plates 14 on both sides.

[0029] Furthermore, such as Figure 4 , Figure 5 As shown, an elastic sheet 3 is fixedly connected to the side wall of the first scraper 17; multiple elastic rods 31 are fixedly connected to the inner side wall of the reactor body 1; the elastic rods 31 are evenly distributed on the inner side wall of the reactor body 1 and have the same structure; a counterweight ball 32 is fixedly connected to the end of the elastic rod 31; during use, when the first scraper 17 rotates, when the elastic sheet 3 moves to the position of the counterweight ball 32, the elastic sheet 3 will hit the wall of the counterweight ball 32, causing the elastic rod 31 to deform to a certain extent. At this time, the impact can cause the elastic sheet 3 to swing and generate a certain vibration effect. The vibration effect can shake off the protein attached to the wall of the first scraper 17. In this process, the counterweight ball 32 knocks on the elastic sheet 3 to make it vibrate, which can clean the protein attached to the wall of the first scraper 17, reduce the protein adhesion, and thus increase the subsequent scraping effect of the first scraper 17.

[0030] Furthermore, such as Figure 5 , Figure 7 As shown, a pair of limiting blocks 4 are fixedly connected to the side wall of the mounting block 11; the limiting blocks 4 are symmetrically arranged on both sides of the mounting block 11 and have the same structure; the side wall of the limiting block 4 has multiple slots 41; the slots 41 are evenly distributed on the side wall of the limiting block 4 and have the same structure; the side wall of the slot 41 is rotatably connected to a roller 42; in use, when the fixing block 13 is inserted into the side wall of the insertion hole 12, the fixing block 13 can contact the two limiting blocks 4 on both sides, and slide towards the middle of the two limiting blocks 4. At the same time, the contact between the fixing block 13 and the roller 42 can drive the roller 42 to rotate, thereby reducing the friction between the fixing block 13 and the limiting block 4. In this process, it can play a limiting role when the fixing block 13 is inserted into the side wall of the insertion hole 12, improving the convenience of inserting the fixing block 13. At the same time, the roller 42 can increase the smoothness of the fixing block 13 sliding on the side wall of the limiting block 4.

[0031] Furthermore, such as Figure 2 As shown, a pair of supports 5 are fixed to the inner wall of the reactor body 1; the supports 5 are symmetrically arranged on both sides of the reactor body 1 and have the same structure; a second scraper 51 is fixed to the end of the support 5; during use, when the stirring mechanism rotates, it will drive the second scraper 51 to rotate at the same time. At this time, the second scraper 51 will scrape off the protein attached to the bottom of the reactor body 1. In this process, the second scraper 51 can clean the protein attached to the bottom of the reactor body 1, improve the cleaning effect inside the reactor body 1, and thus increase the cleanliness of the reactor body 1 when it is used again.

[0032] Furthermore, such as Figure 4 As shown, a rubber sleeve 6 is fixed to the side wall of the counterweight ball 32. In use, when the counterweight ball 32 impacts the elastic sheet 3, the rubber sleeve 6 can be placed between the two. The flexibility of the rubber sleeve 6 can protect the elastic sheet 3. In this process, the rubber sleeve 6 can protect the elastic sheet 3 when it is impacted, reduce the damage caused by the counterweight ball 32 impacting the elastic sheet 3, and thus improve the service life of the elastic sheet 3.

[0033] Working principle: In use, by inserting the fixing block 13 into the side wall of the insertion hole 12, the elastic plate 14 is compressed and adheres to the side wall of the fixing block 13. After the elastic plate 14 passes through the insertion hole 12, it will automatically reset and abut against the side wall of the mounting block 11 due to its elasticity, thus fixing the fixing frame 15. After the stirring mechanism inside the reactor body 1 has completed stirring and mixing and the protein has been discharged, the stirring mechanism is restarted, which causes the first scraper 17 to rotate. At this time, the first scraper 17 adheres to the inner side wall of the reactor body 1, removing the protein adhering to the inner side of the reactor body 1. The protein on the wall is scraped off, causing it to fall to the bottom of the reactor body 1 and finally discharged to the outside. Simultaneously, as the first scraper 17 fully adheres to the inner wall of the reactor body 1, the scraping action causes wear on the first scraper 17. At this point, the elasticity of the spring rod 16 pushes the first scraper 17, ensuring it remains in full contact with the inner wall of the reactor body 1, thus completing the cleaning of the protein adhering to the inner wall of the reactor body 1. During use, pressing the pressing rod 22 moves it into the groove 2. This pressing rod 22 compresses the spring 21, simultaneously moving the connecting ropes 23 on both sides, thus affecting the elastic plate. Pull 14 to remove the fixing block 13 from the side wall of the insertion hole 12. Then release the pressing rod 22. The elasticity of the spring 21 will automatically reset the pressing rod 22. During use, when the first scraper 17 rotates, the elastic plate 3 moves to the position of the counterweight ball 32. The elastic plate 3 will impact the wall of the counterweight ball 32, causing the elastic rod 31 to deform. This impact will cause the elastic plate 3 to swing and vibrate. This vibration will dislodge the protein adhering to the wall of the first scraper 17. During use, insert the fixing block 13 into the side wall of the insertion hole 12. When the fixed block 13 is on the wall, it can contact the two side limiting blocks 4 and slide towards the middle of the two side limiting blocks 4. At the same time, the contact between the fixed block 13 and the roller 42 can drive the roller 42 to rotate, thereby reducing the friction between the fixed block 13 and the limiting block 4. When in use, the stirring mechanism will drive the second scraper 51 to rotate when it rotates. At this time, the second scraper 51 will scrape off the protein attached to the bottom of the reactor body 1. When in use, when the counterweight ball 32 hits the elastic sheet 3, the rubber sleeve 6 can be placed between the two. The flexibility of the rubber sleeve 6 can protect the elastic sheet 3.

[0034] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed utility model.

Claims

1. A continuous flow fermentation apparatus for recombinant protein production, comprising a reactor body (1); characterized in that: A pair of mounting blocks (11) are fixedly connected to the inner side wall of the reactor body (1); the mounting blocks (11) are symmetrically arranged on both sides of the reactor body (1) and have the same structure; the mounting blocks (11) have insertion holes (12) on their side walls; the insertion holes (12) are slidably connected to the side walls of their side walls and have fixing blocks (13); the fixing blocks (13) have a pair of elastic plates (14) symmetrically fixedly connected to their side walls; the fixing blocks (13) have a fixing frame (15) fixedly connected to their side walls; the fixing frame (15) has a pair of spring rods (16) fixedly connected to their side walls; the spring rods (16) are symmetrically arranged on both sides of the fixing frame (15) and have the same structure; the ends of the spring rods (16) are fixedly connected to the same first scraper (17).

2. The continuous flow fermentation apparatus for recombinant protein production as described in claim 1, characterized in that: The side wall of the fixing block (13) has a groove (2); a spring (21) is fixedly connected to the side wall of the groove (2); a pressing rod (22) is fixedly connected to the end of the spring (21); a connecting rope (23) is fixedly connected between the pressing rod (22) and the elastic plate (14); the connecting rope (23) passes through the wall of the fixing block (13) and is slidably connected to it.

3. The continuous flow fermentation apparatus for recombinant protein production as described in claim 1, characterized in that: The first scraper (17) has an elastic sheet (3) fixed to its side wall; the reactor body (1) has a plurality of elastic rods (31) fixed to its inner side wall; the elastic rods (31) are evenly distributed on the inner side wall of the reactor body (1) and have the same structure; the end of the elastic rod (31) is fixed to a counterweight ball (32).

4. The continuous flow fermentation apparatus for recombinant protein production as described in claim 1, characterized in that: A pair of limiting blocks (4) are fixed to the side wall of the mounting block (11); the limiting blocks (4) are symmetrically arranged on both sides of the mounting block (11) and have the same structure; the side wall of the limiting block (4) is provided with multiple slots (41); the slots (41) are evenly distributed on the side wall of the limiting block (4) and have the same structure; the side wall of the slot (41) is rotatably connected to a roller (42).

5. The continuous flow fermentation apparatus for recombinant protein production as described in claim 1, characterized in that: A pair of supports (5) are fixed to the inner wall of the reactor body (1); the supports (5) are symmetrically arranged on both sides of the reactor body (1) and have the same structure; a second scraper (51) is fixed to the end of the support (5).

6. The continuous flow fermentation apparatus for recombinant protein production as described in claim 3, characterized in that: A rubber sleeve (6) is fixed to the side wall of the counterweight ball (32).

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