A stem cell cryo tube

Abstract

The utility model relates to the field of stem cell cryopreservation technology, concretely is a kind of stem cell cryopreservation tube, including shell, the shell top is equipped with top cover by screw thread, the top cover middle part has through-hole, the top cover is equipped with the stem pipe that inserts into the shell inside, the size of stem pipe is less than the size of shell, the gap between stem pipe outer wall and shell inner wall is formed, stem pipe top and top cover are connected, the cavity in stem pipe inside is connected with outside by through-hole. By being provided with stem pipe in shell inside, stem pipe top end and top cover are integrated structure, and stem pipe is connected with outside by the through-hole on top cover, ensure when water bath heating is carried out to cryopreservation tube, warm water can be heated to the inside and outside of stem cell cryopreservation sample simultaneously, both improve heating efficiency, and can ensure the activity after stem cell recovery.

Description

Technical Field

[0001] This utility model relates to the field of stem cell cryopreservation technology, specifically a stem cell cryopreservation tube. Background Technology

[0002] In recent years, stem cell technology has developed rapidly in regenerative medicine, disease treatment, and other fields. The clinical translational applications of mesenchymal stem cells (MSCs) and induced pluripotent stem cells (iPSCs) are becoming increasingly widespread, driving the demand for large-scale stem cell sample storage. Cryopreservation technology, as a core step in maintaining stem cell viability, is becoming increasingly important. After preparation, cryopreserved stem cell samples must be stored in cryovials at ultra-low temperatures to prevent loss of activity. Before use, cryopreserved stem cell samples must be removed from the ultra-low temperature environment and then rapidly thawed in a 37°C water bath. The thawing process must be completed quickly; otherwise, cell death and reduced viability will occur.

[0003] In the current process of thawing, the side of the cryopreservation tube closest to the tube wall thaws first. Since heat transfer takes time, the sample in the center of the cryopreservation tube thaws later. The thicker the cryopreservation tube and the more stem cells it stores, the longer it takes for the sample in the center of the cryopreservation tube to thaw. Under such a large temperature difference, the stem cells will be severely damaged and their viability will be greatly reduced. Utility Model Content

[0004] The technical problem to be solved by this utility model is to overcome the shortcomings of the prior art and provide a stem cell cryopreservation tube. By setting a core tube inside the shell, the top of the core tube and the top cover are integrated into one structure, and the core tube is connected to the outside through the through hole on the top cover, it is ensured that when the cryopreservation tube is heated in a water bath, the warm water can heat the inside and outside of the stem cell cryopreservation sample at the same time, which not only improves the heating efficiency, but also ensures the activity of stem cells after thawing.

[0005] To achieve the above objectives, this utility model provides the following technical solution:

[0006] A stem cell cryopreservation tube includes a shell, a top cover threaded onto the top of the shell, a through hole in the middle of the top cover, a core tube extending into the shell on the top cover, the core tube being smaller than the shell, a gap forming between the outer wall of the core tube and the inner wall of the shell, the top of the core tube being connected to the top cover, and the cavity inside the core tube communicating with the outside through the through hole.

[0007] Preferably, the core tube and the top cover are an integral structure, and the top cover has an installation groove at the point where it abuts against the housing. The installation groove has an annular sealing gasket that abuts against the opening at the top of the housing.

[0008] Preferably, both the shell and the core tube are made of transparent polypropylene.

[0009] Preferably, the upper end of the outer wall of the core tube is provided with a plurality of limiting grooves in sequence, and an annular sealing ring is provided inside the limiting groove, the outer edge of the sealing ring abutting against the inner wall of the shell.

[0010] Preferably, the sealing ring is made of low-temperature fluorosilicone material.

[0011] Preferably, the lower end of the housing has a scale on its side wall.

[0012] Preferably, the top cover has anti-slip texture.

[0013] Compared with the prior art, the beneficial effects of this utility model are:

[0014] 1. This utility model has a simple structure. By inserting the core tube on the top cover into the shell and tightening the top cover, the cylindrical stem cell cryopreservation sample inside the shell will form a ring-shaped gel layer due to the insertion of the core tube. The entire device is placed in a water bath. By shaking the device laterally, the warm water in the water bath can enter the core tube through the through hole and heat the inside of the stem cell cryopreservation sample through the cavity inside the core tube. At the same time, the warm water can also heat the outside of the stem cell cryopreservation sample through the outer wall of the shell, thereby improving the rewarming efficiency of the stem cell cryopreservation sample, reducing the rewarming time, and ensuring the activity of the stem cells after rewarming.

[0015] 2. The core tube and top cover of this device are an integral structure, ensuring a seamless connection between the core tube and the top cover, ensuring airtightness, and preventing external media from entering the housing. When the top cover is screwed on, the sealing gasket inside the top cover is interference-fitted with the opening at the top of the housing, ensuring the airtightness of the housing. The sealing gasket and the sealing ring are made of the same material to prevent the frozen stem cell samples inside the housing from being contaminated by external media.

[0016] 3. The shell and core tube of this device are both made of transparent polypropylene, which makes it easy to observe the amount of frozen stem cell samples inside the shell and to observe the rewarming of the frozen stem cell samples. The polypropylene material also ensures the mechanical strength of the shell and core tube, while ensuring that the core tube and shell can be used for a long time in the low temperature environment of liquid nitrogen.

[0017] 4. This device has multiple sealing rings on the upper end of the outer wall of the core tube. When the core tube is installed inside the shell, the sealing rings abut against the inner wall of the shell to form a seal. Since there are multiple sealing rings, the sealing performance between the shell and the core tube can be further ensured, avoiding contamination of the frozen stem cell samples by warm water during water bath rewarming and ensuring the rewarming quality of the frozen stem cell samples during water bath rewarming.

[0018] 5. This device can accurately display the amount of stem cells perfused into the shell by setting a scale on the side wall of the shell, which avoids excessive perfusion and leakage of the stem cell cryopreservation sample from the sealing ring when the core tube is inserted into the shell, thus preventing external media from contaminating the stem cell cryopreservation sample. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the overall structure of the present invention. Figure 1 ;

[0020] Figure 2 This is a cross-sectional view of the present invention;

[0021] Figure 3 for Figure 2 A magnified view of the local structure.

[0022] In the diagram: 1. Shell; 2. Top cover; 3. Core tube; 4. Through hole; 5. Mounting groove; 6. Sealing gasket; 7. Limiting groove; 8. Sealing ring; 9. Scale; 10. Anti-slip texture. Detailed Implementation

[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0024] Example 1

[0025] A type of stem cell cryopreservation tube, with the following structure: Figures 1-3 As shown, the device includes a housing 1, a top cover 2 is threaded onto the top of the housing 1, the top cover 2 has a through hole 4 in the middle, and a core tube 3 extending into the housing 1 is provided on the top cover 2. The size of the core tube 3 is smaller than the size of the housing 1, a gap is formed between the outer wall of the core tube 3 and the inner wall of the housing 1, the top of the core tube 3 is connected to the top cover 2, and the cavity inside the core tube 3 communicates with the outside through the through hole 4.

[0026] After the frozen stem cell sample is infused into the housing 1, the core tube 3 on the top cover 2 is inserted into the housing 1. After tightening the top cover 2, the originally cylindrical frozen stem cell sample inside the housing 1 will form a ring-shaped gel layer due to the insertion of the core tube 3. The diameter of the through hole 4 in the middle of the top cover 2 is matched with the diameter of the core tube 3. When it is necessary to heat the frozen stem cell sample inside the device, the entire device is placed in a water bath. By shaking the device laterally, the warm water in the water bath can enter the core tube 3 through the through hole 4 and heat the inside of the frozen stem cell sample through the cavity inside the core tube 3. At the same time, the warm water can also heat the outside of the frozen stem cell sample through the outer wall of the housing 1, improving the rewarming efficiency of the frozen stem cell sample, reducing the rewarming time, and ensuring the activity of the stem cells after rewarming.

[0027] The core tube 3 and the top cover 2 are an integral structure. The top cover 2 has an installation groove 5 at the point where it abuts against the shell 1. The installation groove 5 has an annular sealing gasket 6 that abuts against the top opening of the shell 1.

[0028] The core tube 3 and the top cover 2 are an integral structure, ensuring a seamless connection between the core tube 3 and the top cover 2, ensuring airtightness, and preventing external media from entering the interior of the housing 1. When the top cover 2 is screwed on at the top, the sealing gasket 6 inside the top cover 2 is interference-fitted with the opening at the top of the housing 1, ensuring the airtightness inside the housing 1. The sealing gasket 6 and the sealing retaining ring 8 are made of the same material, preventing the frozen stem cell samples inside the housing 1 from being contaminated by external media.

[0029] Both the shell 1 and the core tube 3 are made of transparent polypropylene.

[0030] Both the shell 1 and the core tube 3 are made of transparent polypropylene, which makes it easy to observe the amount of frozen stem cell samples inside the shell 1 and to observe the rewarming of the frozen stem cell samples. The polypropylene material also ensures the mechanical strength of the shell 1 and the core tube 3, while ensuring that the core tube 3 and the shell 1 can be used for a long time in the low temperature environment of liquid nitrogen.

[0031] The upper end of the outer wall of the core tube 3 is provided with a plurality of limiting grooves 7 in sequence, and the limiting groove 7 is provided with an annular sealing ring 8 inside, and the outer edge of the sealing ring 8 abuts against the inner wall of the shell 1.

[0032] Because the upper end of the outer wall of the core tube 3 is provided with multiple sealing rings 8, when the core tube 3 is installed inside the housing 1, the sealing rings 8 abut against the inner wall of the housing 1 to form a seal. Since there are multiple sealing rings 8, the sealing performance of the gap between the housing 1 and the core tube 3 can be further ensured, so as to avoid the warm water from contaminating the frozen stem cell sample during water bath rewarming and to ensure the rewarming quality of the frozen stem cell sample during water bath rewarming.

[0033] The sealing ring 8 is made of low-temperature fluorosilicone material.

[0034] The sealing ring 8 made of low-temperature fluorosilicone not only ensures elasticity in the low-temperature environment of liquid nitrogen, but also ensures its sealing performance, thus ensuring the service life of this device.

[0035] Example 2

[0036] Based on Embodiment 1, the lower side wall of the housing 1 is provided with a scale 9.

[0037] By setting a scale 9 on the side wall of the shell 1, the amount of stem cells perfused into the shell 1 can be accurately displayed, avoiding excessive perfusion that could cause the stem cell cryopreservation sample to overflow from the sealing ring 8 when the core tube 3 is inserted into the shell 1, thus preventing external media from contaminating the stem cell cryopreservation sample.

[0038] The top cover 2 is provided with anti-slip texture 10.

[0039] Since the top cover 2 needs to be connected to the housing 1 by threads, and the sealing gasket 6 needs to be in an interference fit with the top of the housing 1, the interference fit between the sealing gasket 6 and the top of the housing 1 will increase the friction between the top cover 2 and the housing 1. To prevent the operator from being unable to open the top cover 2 effectively, anti-slip texture 10 is set on the top cover 2 to increase the friction between the operator's hand and the top cover 2, ensuring that the top cover 2 can be opened quickly.

[0040] The above description is merely a preferred embodiment of this utility model, but the protection scope of this utility model is not limited thereto. Any variations, additions, subtractions, or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in this utility model should be included within the protection scope of this utility model. Therefore, the protection scope of this utility model should be determined by the scope of the claims.

Claims

1. A stem cell cryopreservation tube, comprising a housing (1), wherein a top cap (2) is threadedly mounted on the top of the housing (1), characterized in that, The top cover (2) has a through hole (4) in the middle. The top cover (2) is provided with a core tube (3) that extends into the shell (1). The size of the core tube (3) is smaller than the size of the shell (1). A gap is formed between the outer wall of the core tube (3) and the inner wall of the shell (1). The top of the core tube (3) is connected to the top cover (2). The cavity inside the core tube (3) is connected to the outside through the through hole (4).

2. The stem cell cryopreservation tube according to claim 1, characterized in that, The core tube (3) and the top cover (2) are an integral structure. The top cover (2) has an installation groove (5) at the point where it abuts against the shell (1). The installation groove (5) has an annular sealing gasket (6) that abuts against the top opening of the shell (1).

3. The stem cell cryopreservation tube according to claim 1, characterized in that, Both the shell (1) and the core tube (3) are made of transparent polypropylene.

4. The stem cell cryopreservation tube according to claim 1, characterized in that, The upper end of the outer wall of the core tube (3) is provided with a plurality of limiting grooves (7) in sequence. The limiting groove (7) is provided with an annular sealing ring (8). The outer edge of the sealing ring (8) abuts against the inner wall of the shell (1).

5. A stem cell cryopreservation tube according to claim 4, characterized in that, The sealing ring (8) is made of low-temperature fluorosilicone material.

6. A stem cell cryopreservation tube according to claim 1, characterized in that, The lower end of the housing (1) has a scale (9) on its side wall.

7. A stem cell cryopreservation tube according to claim 1, characterized in that, The top cover (2) is provided with anti-slip texture (10).

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