Multi-channel switching chromatographic column thermostated sample injector device

Abstract

Multi-channel switching chromatographic column heat sample injector device relates to the technical field of liquid chromatography analyzer auxiliary equipment, including shell, liquid pump, electric valve, semiconductor refrigerator, electric heater, power switch, bottom plate, temperature switch, sample injector body; Shell, liquid pump, liquid storage tank, high performance liquid chromatography analyzer are installed on the bottom plate respectively; Electric heater, semiconductor refrigeration mechanism is installed at the lower end of the shell, the electric valve has many, many electric valves and liquid pump, sample injector body are installed together. The new type under the joint action of the related mechanism, a plurality of chromatographic columns with different measured objects can be put in at a time, and the chromatographic columns with different measured objects can be conveniently switched to high temperature or low temperature in the shell according to the needs of batch detection, different kinds of measured objects can be fully carried into the detector body by mobile phase, which brings convenience to the detection personnel, and the detection accuracy and efficiency are improved. The new type has good prospect.

Description

Technical Field

[0001] This utility model relates to the technical field of auxiliary equipment for liquid chromatography analyzers, and in particular to a multi-channel switching column heat preservation injector device. Background Technology

[0002] High-performance liquid chromatography (HPLC) is a device that uses gas chromatography theory to detect and analyze substances, based on the classic chromatographic analyzer. Technically, HPLC has higher detection sensitivity than ordinary chromatographic analyzers and can continuously detect effluents. Specifically, when a high-performance liquid chromatograph (HPLC) is working, its accompanying liquid pump draws in the solvent (i.e., the mobile phase) from the reservoir and outputs it to the injector. The analyte (pre-formed into a chromatographic column with the analyte, packing material, and liquid isopropanol) is pre-installed in the HPLC injector (i.e., the injection tube). The mobile phase (a substance that carries the analyte forward during column detection, such as liquid acetonitrile-aqueous solution, acetonitrile-acetic acid-aqueous solution, methanol-aqueous solution, acetonitrile-phosphoric acid-aqueous solution, etc.) is injected through the upper inlet tube of the injector, passes through the chromatographic column, and after separation by the column, the analyte flows out through the lower outlet tube of the injector into the detector of the HPLC. The signal detected by the detector is acquired and processed by the HPLC's data processing equipment, and the chromatogram of the analyte is recorded, thus determining the composition of the analyte.

[0003] Current high-performance liquid chromatography (HPLC) analyzers typically have only one injector. Its structure includes a cylindrical body (with a cross-shaped support at the lower end to prevent the lower end of the column from contacting the lower part of the cylinder after placement, facilitating the inflow and outflow of the mobile phase), a movable cap, and inlet and outlet pipes on the upper and lower sides of the cylinder, respectively. In application, the column is placed inside the cylinder, and the cap is closed. A high-pressure pump then pumps the mobile phase into the cylinder. After the HPLC analyzer finishes analyzing the sample, the movable cap is opened to remove the sample column. While this method meets the detection requirements, it is structurally limited. Because there is only one injector, only one column with different analytes can be analyzed at a time. Furthermore, after analyzing each sample column, the corresponding tubing needs to be cleaned, causing significant inconvenience to the operator. Additionally, when analyzing a relatively large number of columns containing different analytes, the detection efficiency becomes low. More importantly, existing injectors cannot maintain the temperature of the chromatographic column. In reality, different analytes require different column temperatures. Some analytes (such as polystyrene) require a mobile phase of 250°C to effectively carry the analyte forward into the detector, while others (such as polysaccharides) require a lower temperature of 10°C-15°C (generally, indoor temperatures are higher than this, so cooling is necessary, especially outside of winter) for the mobile phase to adequately carry the analyte forward into the detector. Therefore, to achieve good temperature control, injectors are currently installed in insulated or refrigerated chambers. These separate devices cause inconvenience for operators, are less compact, and require more space, potentially negatively impacting the testing process. Utility Model Content

[0004] To overcome the shortcomings of existing high-performance liquid chromatography (HPLC) analyzers due to structural limitations, as described in the background, this invention provides a multi-channel switching column insulated injector device that, with the combined action of related mechanisms, can simultaneously accommodate multiple chromatographic columns with different analytes. Furthermore, it allows for convenient switching between high and low temperatures within the outer shell to accommodate columns with different analytes in batch testing. This ensures that different types of analytes are fully carried by the mobile phase into the detector, thus providing convenience for testing personnel and correspondingly improving detection accuracy and efficiency.

[0005] The technical solution adopted by this utility model to solve its technical problem is:

[0006] A multi-channel switching chromatographic column insulated injector device includes an outer shell, injector body, liquid pump, electric valves, semiconductor cooler, electric heater, base plate, and temperature switch. The outer shell, liquid pump, liquid tank of the high-performance liquid chromatograph (HPLC), and HPLC analyzer are respectively fixedly mounted on the base plate. The electric heater is fixedly mounted on the lower outer side of the outer shell. A ring-shaped hollow magnet is fixedly mounted on the cold end shell of the semiconductor cooler. A contact plate is located on the lower outer side of the outer shell, and a ring-shaped hollow steel suction plate is fixedly mounted on the outer side of the contact plate. The cold end of the semiconductor cooler is attracted to the lower end of the suction plate by the magnet. There are multiple injector bodies, and the lower ends of multiple injector bodies are respectively fixedly mounted in the outer shell. The inlet and outlet pipes of multiple injector bodies are located on both ends of the outer shell. There are eleven electric valves. One end of the first, second, third, and fourth electric valves is fixedly connected to the outer side of the inlet pipes of the four injector bodies. Three electric valves, the other end of the fourth electric valve, and one end of the ninth electric valve are fixedly connected in parallel to the outlet of the liquid pump. The inlet of the liquid pump is fixedly connected to the outlet pipe of the storage tank. One end of the fifth, sixth, seventh, and eighth electric valves is fixedly connected to the outside of the outlet pipes of the four injector bodies. The other ends of the fifth, sixth, seventh, eighth, and ninth electric valves, and one end of the tenth and eleventh electric valves are fixedly connected to the seven ports of a connecting pipe. The other ends of the tenth and eleventh electric valves are connected to the detector of the high-performance liquid chromatograph and the inlet pipe of the mobile phase recovery tank, respectively. There are at least two temperature switches, each installed on the outside of one end of the outer casing. The power output terminal of the first temperature switch is electrically connected to one power input terminal of the semiconductor refrigeration mechanism, and the power output terminal of the second temperature switch is electrically connected to one of the power input terminals of the electric heating tube.

[0007] Furthermore, the outer diameter of the suction plate is the same as the outer diameter of the magnet sheet, and the outer diameter of the contact plate is smaller than the inner diameter of the magnet sheet.

[0008] Furthermore, the cold end of the semiconductor cooling mechanism and the lower end of the contact plate are in close contact, and the outer diameter of the semiconductor cooling mechanism is smaller than the inner diameter of the electric heating tube.

[0009] Furthermore, a movable sealing cover is hinged to the upper end of the outer casing; the probes of the two temperature switches are located inside the outer casing.

[0010] Furthermore, the outer shell is made of copper, and the outer ends of the outer shell and the upper end of the movable sealing cover are respectively covered with thermal insulation material.

[0011] Furthermore, the multiple electric valves can also be replaced by one of the following: a solenoid valve or a multi-way switching valve.

[0012] Furthermore, heat-resistant sealing gaskets are installed at the lower end of the movable sealing cover and the upper end of the outer shell.

[0013] Compared with the prior art, the advantages of this utility model are: (1) This utility model has multiple injector bodies, so before detection, multiple chromatographic columns with different analytes can be placed into the cylinders of multiple injector bodies for detection. The detection personnel can easily switch the power switch to allow the liquids of multiple chromatographic columns carried by the high-pressure mobile phase output by the liquid pump to enter the detector of the high performance liquid chromatograph, and detect the analytes contained in multiple chromatographic columns one by one, and can easily clean the corresponding pipelines; (2) Because it has a semiconductor cooler and an electric heater (the semiconductor can be easily installed and disassembled by magnet, which prevents the electric heating tube from being damaged by high temperature), the detection personnel can easily switch the shell to be at high temperature or low temperature according to the needs of the chromatographic columns with different analytes for batch detection. Different types of analytes can be fully carried into the detector by the mobile phase, which brings convenience to the detection personnel and improves the detection accuracy and efficiency accordingly. Attached Figure Description

[0014] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0015] Figure 1 This is a schematic diagram of the overall structure of this utility model.

[0016] Figure 2 This is a partially enlarged structural schematic diagram of this utility model.

[0017] Figure 3 This is the circuit diagram of this utility model. Detailed Implementation

[0018] Figure 1 , 2As shown in Figure 3, the multi-channel switching chromatographic column insulated injector device includes a power module E1, an outer shell 1, an injector body 2, a liquid pump M, an electric valve DCN, power switches SN (S1, S2, S3), a semiconductor cooler B, a ring heater RT, a base plate 3, and temperature switches T1 and T2. The injector body 2 includes a cylindrical body 21 (with a cross-shaped support frame at the lower end so that the lower end of the chromatographic column does not contact the lower end of the cylinder after the column is placed inside, facilitating the inflow and outflow of the mobile phase liquid) and a movable cover 22. The movable cover 22 is threaded onto the cylinder 21 (the chromatographic column is installed inside the cylinder); four support feet 101 are installed on the lower outer side of the outer shell 1, and the lower ends of the four support feet 101 are respectively fixedly installed on the middle of the base plate 3; the lower end of the liquid pump M is fixedly installed on the base plate at the right end of the outer shell 1; the lower end of the liquid storage tank 4 of the high performance liquid chromatograph is fixedly installed on the base plate at the right end of the liquid pump M; the lower end of the high performance liquid chromatograph (not shown in the figure) is fixedly installed on the left end of the base plate 3; the upper part of the annular electric heater RT... The cold end of the semiconductor cooling mechanism B is fixedly installed on the outer side of the lower end of the outer shell 1. A ring-shaped hollow thin permanent magnet 5 is fixedly installed on the cold end shell of the semiconductor cooling mechanism B. The middle of the lower end of the outer shell 1 has a circular recessed structure as a contact plate 102. A ring-shaped hollow steel suction plate 103 is fixedly installed on the outer side of the contact plate 102. The cold end of the semiconductor cooling mechanism B is attracted to the lower end of the suction plate 103 by the magnet 5, and the middle of the cold end of the semiconductor cooling mechanism B and the lower end of the contact plate 102 are in close contact. The sampler body 2 has four samplers. The lower end of the injector body 2 is fixedly installed in the outer shell 1 in a ring-shaped arrangement. The inlet pipes 23 of the four injector bodies are horizontally distributed front to back and located outside the upper right end of the outer shell 1 (the outer side of the inlet pipe 23 and the inner side of the four openings at the upper right end of the outer shell are sealed). The outlet pipes 24 of the four injector bodies 2 are horizontally distributed front to back and located outside the lower left end of the outer shell 1 (the outer side of the outlet pipe 24 and the inner side of the four openings at the lower left end of the outer shell are sealed). There are eleven electric valves DCN, including four electric valves DCN-1. One end of DCN-2, DCN-3, and DCN-4 and the outside of the liquid inlet pipes 23 of the four injector bodies are fixedly connected by pipes. The other ends of the four electric valves DCN-1, DCN-2, DCN-3, and DCN-4, one end of the ninth electric valve DCN-9, and five of the ports of a six-way connector are fixedly connected by pipes. The sixth port of the six-way connector is connected to the liquid outlet of the liquid pump M by pipe. The liquid inlet of the liquid pump M is fixedly connected to the liquid outlet pipe of the storage tank 4 by pipe.The other four electric valves DCN-5, DCN-6, DCN-7, and DCN-8 are fixedly connected at one end to the outside of the outlet pipe 24 of the four sampler bodies via pipes. The other end of DCN-8, the other end of the ninth electric valve DCN-9, one end of the tenth electric valve DCN-10 and the eleventh electric valve DCN-11, and the seven ports of a connecting pipe 6 are respectively fixedly connected via pipes. The other four electric valves, the other end of the ninth electric valve, and one end of the tenth and eleventh electric valves are interconnected with the connecting pipe. The other ends of the tenth electric valve DCN-10 and the eleventh electric valve DCN-11 are respectively connected via pipes to the detector inlet pipe and the mobile phase recovery tank inlet pipe (not shown in the figure) of the high-performance liquid chromatograph. There are two temperature switches T1 and T2, which are respectively sealed and fixedly installed in the middle of the right outer side of the outer casing 1. The power module E1 and the power switch are installed in the component box 7, which is installed on the front side of the middle of the base plate 3.

[0019] Figure 1 , 2 As shown in Figure 3, the outer diameter of the suction plate 103 is the same as the outer diameter of the magnet piece 5, and the outer diameter of the contact plate 102 is smaller than the inner diameter of the magnet piece 5. The middle part of the cold end of the semiconductor cooling mechanism B is in close contact with the lower end of the contact plate 102, and the outer diameter of the semiconductor cooling mechanism B is smaller than the inner diameter of the electric heating tube RT. A movable sealing cover 104 is hinged to the upper end of the outer casing 1; the probes of the two temperature switches T1 and T2 are located inside the right end of the outer casing 1. The outer casing 1 is made of copper, and the outer ends of the outer casing 1 and the upper end of the movable sealing cover 104 are respectively covered with glass wool insulation material. Multiple electric valves DCN can also be replaced by a switching solenoid valve or a multi-way switching valve. Heat-resistant sealing glass wool gaskets (for sealing) are installed at the lower end of the movable sealing cover 104 and the upper end of the outer casing.

[0020] Figure 1 , 2As shown in Figure 3, the power input terminals 1 and 2 of the power module E1 are connected to the two poles of the 220V AC power supply via wires. The power output terminals 3 and 4 of the power module E1 are connected to the power input terminals 1 and 2 of the eleven power switches SN via wires. The power output terminals 3, 4, 5, and 6 of the eleven power switches E1 are connected to the power input terminals of the eleven electric valves DCN via wires. The power input terminal of the twelfth power switch S1 is connected to the positive terminal 3 of the power output terminal of the power module E1 via wires. The power output terminals of the twelve power switches S1 are connected to the power input terminal of the first temperature switch T1 via wires. The power output terminal of the first temperature switch T1, the negative power output terminal 4 of the power module E1, and a power socket located outside the component box are connected via wires. The two terminals are connected by wires. The two power input terminals of the semiconductor refrigeration mechanism B and the two terminals of a power plug are connected by wires. The power plug is inserted into the power socket. The power input terminals of the thirteenth power switch S2 and the fourteenth power switch S3 are connected to one pole of the 220V AC power supply by wires. The power output terminal of the fourteenth power switch S3 is connected to one of the power input terminals of the liquid pump M by wires. The power output terminal of the thirteenth power switch S2 and the power input terminal of the second temperature switch T2 are connected by wires. The power output terminal of the second temperature switch T2 is connected to one of the power input terminals of the electric heating tube RT by wires. The other power input terminal of the electric heating tube RT and the liquid pump M is connected to the other pole of the 220V AC power supply by wires.

[0021] Figure 1 , 2As shown in Figure 3, after the 220V AC power enters the power input terminal of the power module E1, the power output terminal of the power module E1 outputs a DC 12V power supply which enters the power input terminals of the eleventh and twelfth power switches (one pole of the 220V AC power enters the power input terminals of the thirteenth and fourteenth power switches). When a mobile phase containing the analyte is required to be output from the corresponding injector body within the outer casing (entering the detector of the high-performance liquid chromatograph), the operator turns on the power switch S3 of the hydraulic pump M and moves the power switch SN of the tenth electric valve DCN-10 or the power switch SN of the second electric valve DCN-2, the sixth electric valve DCN-6, the third electric valve DCN-3, the seventh electric valve DCN-7, the fourth electric valve DCN-4, and the eighth electric valve DCN-8 to the left. Pins 1 and 2, and pins 3 and 4 of the aforementioned power switches SN are connected respectively. In this way, the energized valve cores of the corresponding electric valves open, and the mobile phase pumped out by the liquid pump M under pressure enters the detector through the tenth electric valve via the first and fifth electric valves or the second and sixth electric valves, the third and sixth electric valves, the seventh and eighth electric valves. When cleaning residual mobile phase containing the analyte in the pipeline between the other end of the fifth electric valve DCN-5, or the sixth electric valve DCN-6, the seventh electric valve DCN-7, the eighth electric valve DCN-8, and the tenth electric valve DCN-10 (to prevent the previous substance from affecting the detection of the second substance), the inspector should turn the power switch handles of the first electric valve DCN-1 and the fifth electric valve DCN-5, or the second electric valve DCN-2 and the sixth electric valve DCN-6, the third electric valve DCN-3 and the seventh electric valve DCN-7, the fourth electric valve DCN-4 and the eighth electric valve DCN-8 to the right. This connects pins 1 and 2, and pins 5 and 6 of the aforementioned power switches SN, thus energizing the corresponding electric valves. The power input valve is energized and closed. Then, the power switch S3 of the hydraulic pump M is turned on, and the power switches SN of the eleventh electric valve DCN-11 and the ninth electric valve DCN-9 are flipped to the left. Pins 1 and 2, and pins 3 and 4 of the power switches SN are connected respectively. This opens the energized valves at the positive and negative power input terminals of the corresponding electric valves. The liquid pump M extracts and pressurizes the mobile phase, which is then output to the mobile phase recovery tank via the ninth electric valve DCN-9 and the eleventh electric valve DCN-11. The flow of the mobile phase in and out cleans the residual mobile phase containing the analyte in the pipeline between the other end of the fifth electric valve DCN-5 or the sixth electric valve DCN-6, the seventh electric valve DCN-7, the eighth electric valve DCN-8, and the tenth electric valve DCN-10. After cleaning, all power switches are turned off.

[0022] Figure 1 ,2 As shown in Figure 3, when it is necessary to heat multiple injector bodies inside the outer shell, the power switch S2 is turned on. When the temperature inside the outer shell is below 250°C, the internal contacts of the temperature switch T2 are closed, so the electric heating tube RT is energized and heats the injector body inside the outer shell. When the temperature inside the outer shell is above 250°C (the temperature is adjustable), the internal contacts of the temperature switch T2 are open, so the electric heating tube RT is de-energized and no longer heats the injector body inside the outer shell. Through the above, this new invention can keep the injector body inside the outer shell at a high temperature. When it is necessary to cool down multiple injector bodies inside the housing, turn on the power switch S1 (turn off S2), attach the semiconductor cooling mechanism B to the lower end of the housing, connect the power plug and the power socket. When the temperature inside the housing is higher than 15°C, the internal contacts of the temperature switch T1 close, thus energizing the semiconductor cooling mechanism B to cool down the injector bodies inside the housing. When the temperature inside the housing is lower than 15°C, the internal contacts of the temperature switch T1 open, thus de-energizing the semiconductor cooling mechanism B and ceasing to cool the injector bodies inside the housing. Through the above, this invention can keep the injector bodies inside the housing at a low temperature until the power switch S1 is turned off.

[0023] Figure 1 , 2 As shown in Figure 3, this novel device allows for the placement of multiple chromatographic columns with different analytes into the cylinders of multiple injectors before detection. By simply switching the power switch, the operator can easily direct the various column liquids carried by the high-pressure mobile phase from the liquid pump into the detector of the high-performance liquid chromatograph (HPLC), enabling the individual detection of the analytes contained in each column. This also facilitates the cleaning of the corresponding tubing. The device incorporates a semiconductor cooler and an electric heater (the semiconductor cooler can be magnetically attached and disassembled, preventing damage from high temperatures). The operator can easily switch the temperature of the outer casing between high and low temperatures according to the needs of batch testing of columns with different analytes. Different types of analytes can be fully carried by the mobile phase into the detector, thus providing convenience and improving detection accuracy and efficiency. Figure 3 In the middle, power module E1 is a finished product of AC 220V to DC 12V power module; electric valve DCN power 2W; semiconductor refrigeration mechanism B power 200W; electric heating tube RT power 1KW; liquid pump M is a 500W self-priming liquid pump; temperature switches T1 (internal contact normally closed) and T2 (internal contact normally open) are finished products of adjustable temperature switches.

[0024] Those skilled in the art should understand that although this specification describes embodiments, the embodiments do not necessarily contain only one independent technical solution. This way of describing the specification is only for clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in the embodiments can also be appropriately combined to form other embodiments that can be understood by those skilled in the art. Therefore, the scope of protection of this application is defined by the claims.

Claims

1. A multi-channel switching chromatographic column heated injector device, comprising an outer shell, an injector body, a liquid pump, an electric valve, a semiconductor cooler, an electric heater, a base plate, and a temperature switch; characterized in that, The outer casing, liquid pump, high-performance liquid chromatograph (HPLC) reservoir, and HPLC analyzer are all fixedly mounted on the base plate. The electric heater is fixedly mounted on the lower outer side of the outer casing. A ring-shaped hollow magnet is fixedly mounted on the cold end shell of the semiconductor refrigeration mechanism. A contact plate is located on the lower outer side of the outer casing, and a ring-shaped hollow steel suction plate is fixedly mounted on the outer side of the contact plate. The cold end of the semiconductor refrigeration mechanism is attracted to the lower end of the suction plate by the magnet. There are multiple injector bodies, and the lower ends of multiple injector bodies are fixedly mounted inside the outer casing. The inlet and outlet pipes of multiple injector bodies are located at both ends of the outer casing. There are eleven electric valves. One end of the first, second, third, and fourth electric valves is fixedly connected to the outer side of the inlet pipes of the four injector bodies. The other ends of the first, second, third, and fourth electric valves and one end of the ninth electric valve are fixedly connected to the other end of the first, second, third, and fourth electric valves. The liquid pump's outlet end is fixedly connected in parallel, and the liquid pump's inlet end is fixedly connected to the outlet pipe of the storage tank. One end of the fifth, sixth, seventh, and eighth electric valves is fixedly connected to the outside of the outlet pipes of the four injector bodies. The other ends of the fifth, sixth, seventh, eighth, and ninth electric valves, and one end of the tenth and eleventh electric valves, are fixedly connected to the seven ports of a connecting pipe. The other ends of the tenth and eleventh electric valves are connected to the inlet pipes of the detector and mobile phase recovery tank of the high-performance liquid chromatograph, respectively. There are at least two temperature switches, each installed on the outside of one end of the outer casing. The power output terminal of the first temperature switch is electrically connected to one power input terminal of the semiconductor refrigeration mechanism, and the power output terminal of the second temperature switch is electrically connected to one of the power input terminals of the electric heating tube.

2. The multi-channel switching chromatographic column heat-insulating injector device according to claim 1, characterized in that, The outer diameter of the suction plate is the same as the outer diameter of the magnet, while the outer diameter of the contact plate is smaller than the inner diameter of the magnet.

3. The multi-channel switching chromatographic column heat-insulating injector device according to claim 1, characterized in that, The cold end of the semiconductor cooling mechanism and the lower end of the contact plate are in close contact, and the outer diameter of the semiconductor cooling mechanism is smaller than the inner diameter of the electric heating tube.

4. The multi-channel switching chromatographic column heat-insulating injector device according to claim 1, characterized in that, A movable sealing cover is hinged to the upper end of the outer casing; the probes of the two temperature switches are located inside the outer casing.

5. The multi-channel switching chromatographic column heat-insulating injector device according to claim 1, characterized in that, The outer shell is made of copper, and the outer ends of the outer shell and the upper end of the movable sealing cover are covered with thermal insulation material.

6. The multi-channel switching chromatographic column heat-insulating injector device according to claim 1, characterized in that, Multiple electric valves can also be replaced by either a solenoid valve or a multi-way switching valve.

7. The multi-channel switching chromatographic column heat-insulating injector device according to claim 1, characterized in that, Heat-resistant sealing gaskets are installed at the lower end of the movable sealing cover and the upper end of the outer shell.

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