METHODS AND KITS FOR DEFINITION AND ENRICHEMENT OF NUCLEAR ACID SEQUALITIES
Patent Information
- Application Number
- DE602019067910
- Authority / Receiving Office
- DE · DE
- Patent Type
- Patents
- Current Assignee / Owner
- Priority Date
- 2018-10-24
- Filing Date
- 2019-10-24
- Publication Date
- 2025-03-26
- Estimated Expiration
- 2039-10-24
AI Technical Summary
Current sequencing methods for complex biological samples generate excess non-target nucleic acid sequences, making it difficult to determine target sequences and concentrations, and there is a need to selectively enrich or deplete target nucleic acid molecules to simplify data interpretation and reduce sequencing depth.
The method involves introducing a capture primer nucleic acid molecule complementary to a target sequence into a sample solution, enzymatically extending it, and then degrading single-stranded or double-stranded nucleic acid molecules to enrich or deplete the target sequence, using enzymes like Rnase T1 or Rnase A, and universal adaptor sequences to facilitate the process.
This approach effectively increases the proportion of target nucleic acid molecules in the sample solution, reducing the need for excessive sequencing reads and improving data interpretation by selectively removing non-target sequences, thereby enhancing sequencing efficiency.