Concentrated serum delivery for skin treatment

Abstract

According to some embodiments, a device for treating a skin surface comprises a handpiece with an opening configured to receive a container containing a concentrated treatment fluid, a skin interface portion, configured to contact the skin surface, and a treatment fluid mixing zone within the handpiece. The mixing zone can receive concentrated treatment material from the container secured to the handpiece and dilution fluid from a dilution fluid conduit, and mix the two or more input fluids / materials to create a diluted treatment fluid to be delivered to the interface portion during use. The handpiece can include a waste fluid connection in fluid communication with the interface portion of the device and a vacuum source to draw the mixed treatment fluid to the interface portion and skin tissue, and to draw spent treatment material from the interface portion during use.

Description

EDGE.222WO PATENT APPLICATIONCONCENTRATED SERUM DELIVERY FOR SKIN TREATMENTRelated Applications

[0001] This application claims priority to U.S. Provisional Patent Application No. 63 / 516804, filed July 31, 2023, the entire contents of which are incorporated herein by reference and made a part of this application.Field

[0002] This application relates generally to skin treatment, and more specifically, to dilution of a concentrate or concentrated treatment fluid during a skin treatment procedure. Description of the Related Art

[0003] Abrasion of the outer layer or epidermis of the skin is desirable to smooth or blend scars, blemishes, or other skin conditions that may be caused by, for example, acne, sun exposure, and aging. Standard techniques used to abrade the skin have generally been separated into two fields referred to as dermabrasion and microdermabrasion. Both techniques remove portions of the epidermis called the stratum comeum, which the body interprets as a mild injury. The body then replaces the lost skin cells, resulting in a new outer layer of skin. Additionally, despite the mild edema and erythema associated with the procedures, the skin looks and feels smoother because of the new outer layer of skin.

[0004] Dermabrasion generally refers to a procedure in which the outer surface of the skin is removed due to mechanical rubbing by a handpiece with an abrasive element that is often in the form of a burr, wheel, disc or the like. This process tends to be messy and painful, sometimes necessitating the administration of a local anesthetic to the person being treated. In general, dermabrasion leaves the skin red and raw-looking. The removed skin can take several months to regrow and heal. Recent efforts have led to the use of lasers instead of abrasive elements, resulting in less bleeding. However, the pain and messiness of such procedures normally remain.

[0005] Efforts have been made to decrease the mess caused by the process waste, such as, for example, removed skin, blood, other debris and the like, by adding a suction element. As the process waste is drawn into the suction opening, skin that has not been removed is also pulled against the grit surrounding the suction opening, so the procedure remains relatively messy due to the abrasion that takes place outside of the handpiece by the grit.EDGE.222WO -1- Knobbe, Martens, Olson & Bear, LLP

[0006] In general, microdermabrasion refers generally to a procedure in which the surface of the skin is removed by mechanical rubbing using a handpiece that can discharge a stream of sand or grit. For example, a handpiece can be used to direct a fluid containing crystals of aluminum oxide, sodium chloride and / or sodium bicarbonate. The velocity and momentum of the grit helps wear away cell layers of the skin with each pass of the handpiece. Alternatively, new “crystal-free” microdermabrasion techniques utilize a diamond-tipped handpiece without a stream of grit.

[0007] Efforts to add a suction element have been more successful in microdermabrasion than in dermabrasion, because the handpiece applying the stream of grit is more controllable to a localized area. That is, as the removed skin is drawn into the suction opening, skin that has not been removed is also pulled towards the handpiece where it is treated with the grit stream, allowing for simultaneous local treatment and suction.

[0008] Microdermabrasion typically removes moisture from the skin. Thus, the procedure is generally followed by the application of moisturizing creams, other agents and / or other materials. However, similar to topical application of moisturizing creams prior to microdermabrasion, the moisturizing elements only work as deep as the active ingredients can passively migrate through the remaining epidermis.Summary

[0009] During dermabrasion or microdermabrasion procedures, treatment fluid can be included to aid in the abrasion procedure, hydrate or nourish the skin, or for other purposes for skin treatment. Distribution of bulky containers of treatment fluid at the correct concentration for use in a skin treatment procedure increases costs and impacts possible distributors or performers of the skin treatment system. Thus, a need is felt in the field for a more portable solution to administer treatment fluid to the skin of a patient.

[0010] In some embodiments, the techniques described herein relate to a device for treating a skin surface, including: a handpiece having a distal end and a proximal end of an handpiece assembly; wherein the handpiece includes an opening configured to receive a container, the container configured to contain a concentrated treatment material; an interface portion configured to be positioned along the distal end of the handpiece, said interface portion being configured to contact the skin surface; a mixing zone within the handpiece, the mixingEDGE.222WO -2- Knobbe, Martens, Olson & Bear, LLPzone configured to receive (i) a volume of concentrated treatment material from the container secured to the opening of the handpiece and (ii) a volume of dilution fluid from a dilution fluid conduit; wherein a mixture of the concentrated treatment material and the dilution fluid creates a diluted treatment fluid configured to be delivered to the interface portion during use; a waste fluid connection in fluid communication with the interface portion of the device and a vacuum source, the waste fluid connection configured to removably attach to a waste fluid conduit; a dilution fluid connection in fluid communication with the mixing zone, the dilution fluid connection configured to removably attach to the dilution fluid conduit, and at least one delivery conduit configured to transfer the diluted treatment fluid from the mixing zone to the distal end of the handpiece when the interface portion is positioned along the skin surface and the vacuum source is activated, thus placing the mixing zone in fluid communication with the interface portion.

[0011] In some embodiments, the techniques described herein relate to a device, wherein: the mixing zone includes a plurality of interference surfaces or features, the plurality of interference surfaces or features including planar or curved surfaces that create a tortuous fluid path to promote mixing create a spiral flow path; wherein a ratio of the dilution fluid and the concentrated treatment material is determined before a treatment procedure is commenced or performed; and wherein the ratio of the dilution fluid and the concentrated treatment material is controlled, at least in part, by a flow control device, component or feature associated with the dilution fluid connection.

[0012] In some embodiments, the techniques described herein relate to a device, further including: at least one sensor located within or downstream from the mixing zone to measure or estimate a concentration of the diluted treatment fluid located within or exiting the mixing zone and generate concentration data; wherein the concentration data provided by the at least one sensor are used to regulate at least one of a flowrate of the dilution fluid and a flowrate of the concentrated treatment material entering the mixing zone; wherein the at least one sensor is configured to transmit concentration data to a control unit; and a feedback loop control system, wherein the feedback loop control system receives data regarding the concentration of the diluted treatment fluid; and wherein the feedback loop control system modifies an amount of the dilution fluid and / or concentrated treatment material provided to the mixing zone.EDGE.222WO -3- Knobbe, Martens, Olson & Bear, LLP

[0013] In some embodiments, the techniques described herein relate to a device, further including a flow control device, wherein the flow control device is configured to be handled and selectively moved by a user of the device, and wherein the flow control device regulates a flowrate of diluted treatment fluid from the mixing zone to the distal end of the handpiece.

[0014] In some embodiments, the techniques described herein relate to a device, further including a diluted treatment fluid concentration control device, wherein the diluted treatment fluid concentration control device is configured to be handled and selectively moved by a user of the device, and wherein the diluted treatment fluid concentration control device regulates at least one of a flowrate of the concentrated treatment material to the mixing zone and a flow rate of dilution fluid to the mixing zone.

[0015] In some embodiments, the techniques described herein relate to a device, wherein the mixing zone includes a micromixer or other features that create a tortuous fluid path to promote mixing.

[0016] In some embodiments, the techniques described herein relate to a device, wherein the mixing zone includes a plurality of interference surfaces or features.

[0017] In some embodiments, the techniques described herein relate to a device, wherein the plurality of interference surfaces or features compromise planar or curved surfaces.

[0018] In some embodiments, the techniques described herein relate to a device, wherein the micromixer or other features that create the tortuous fluid path to promote mixing create a spiral flow path.

[0019] In some embodiments, the techniques described herein relate to a device, wherein the micromixer or other features that create the tortuous fluid path to promote mixing includes perforated surfaces crossing the mixing zone.

[0020] In some embodiments, the techniques described herein relate to a device, wherein the micromixer or other features that create the tortuous fluid path to promote mixing includes at least one flexible diaphragms configured to move between at least two positions to promote mixing.

[0021] In some embodiments, the techniques described herein relate to a device, wherein a ratio of the dilution fluid and the concentrated treatment material is determined before a treatment procedure is commenced or performed.EDGE.222WO -4- Knobbe, Martens, Olson & Bear, LLP

[0022] In some embodiments, the techniques described herein relate to a device, wherein the ratio of the dilution fluid and the concentrated treatment material is controlled, at least in part, by a flow control device, component or feature associated with the dilution fluid connection.

[0023] In some embodiments, the techniques described herein relate to a device, wherein the ratio of the dilution fluid and the concentrated treatment material is manually controlled.

[0024] In some embodiments, the techniques described herein relate to a device, wherein the ratio of the dilution fluid and the concentrated treatment material is automatically controlled.

[0025] In some embodiments, the techniques described herein relate to a device, wherein the ratio of the dilution fluid and the concentrated treatment material is controlled, at least in part, using a feedback loop control system.

[0026] In some embodiments, the techniques described herein relate to a device, wherein the feedback loop control system includes at least one sensor, the at least one sensor being configured to detect or approximate a concentration of the diluted treatment fluid exiting the mixing zone.

[0027] In some embodiments, the techniques described herein relate to a device, wherein the mixing zone includes a mixing chamber.

[0028] In some embodiments, the techniques described herein relate to a device, wherein the concentrated treatment material and the dilution fluid are mixed by being directed in non-parallel directions when entering the mixing chamber.

[0029] In some embodiments, the techniques described herein relate to a device, wherein the mixing zone further includes a second treatment concentrate connection for an additional concentrated treatment .

[0030] In some embodiments, the techniques described herein relate to a device, wherein the device is configured for microdermabrasion.

[0031] In some embodiments, the techniques described herein relate to a device, further including at least one sensor located within or downstream from the mixing zone to measure or estimate a concentration of the diluted treatment fluid located within or exiting the mixing zone and generate concentration data.EDGE.222WO -5- Knobbe, Martens, Olson & Bear, LLP

[0032] In some embodiments, the techniques described herein relate to a device, wherein the concentration data provided by the at least one sensor arc used to regulate at least one of a flowrate of the dilution fluid and a flowrate of the concentrated treatment material entering the mixing zone.

[0033] In some embodiments, the techniques described herein relate to a device, wherein the at least one sensor is configured to transmit concentration data to a control unit.

[0034] In some embodiments, the techniques described herein relate to a device, wherein the control unit is included within the handpiece.

[0035] In some embodiments, the techniques described herein relate to a device, wherein the control unit is external to the handpiece.

[0036] In some embodiments, the techniques described herein relate to a device accordingly to any one to 26, further including a feedback loop control system, wherein the feedback loop control system receives data regarding the concentration of the diluted treatment fluid; and wherein the feedback loop control system modifies an amount of the dilution fluid and / or concentrated treatment material provided to the mixing zone.

[0037] In some embodiments, the techniques described herein relate to a device, wherein the mixing zone is configured to promote mixing of the dilution fluid and the concentrated treatment material.

[0038] In some embodiments, the techniques described herein relate to a device, wherein the interface portion includes a tip.

[0039] In some embodiments, the techniques described herein relate to a device, wherein the tip is removable from the handpiece.

[0040] In some embodiments, the techniques described herein relate to a device, wherein the interface portion is removable from the device.

[0041] In some embodiments, the techniques described herein relate to a device, further including the dilution fluid conduit.

[0042] In some embodiments, the techniques described herein relate to a device, further including the waste fluid conduit configured to generate a vacuum from the vacuum source.

[0043] In some embodiments, the techniques described herein relate to a device, wherein the container includes a single chamber.EDGE.222WO -6- Knobbe, Martens, Olson & Bear, LLP

[0044] In some embodiments, the techniques described herein relate to a device, wherein the container includes at least two chambers, each of said at least two chambers being fluidly distinct of each other.

[0045] In some embodiments, the techniques described herein relate to a device, wherein the container includes at least three chambers.

[0046] In some embodiments, the techniques described herein relate to a device, wherein at least one of the concentrated treatment material reacts with the dilution fluid to effervesce.

[0047] In some embodiments, the techniques described herein relate to a device, wherein at least one of the concentrated treatment material reacts with the dilution fluid to increase its efficacy during a treatment procedure

[0048] In some embodiments, the techniques described herein relate to a device for treating a skin surface, including: a handpiece having a distal end and a proximal end, said handpiece including at least one delivery conduit and at least one waste conduit; a tip configured to be positioned along the distal end of the handpiece, said tip being configured to contact the skin surface; a micromixer including an inlet and an outlet, the outlet being fluidly connected to the at least one delivery conduit, wherein the inlet of the micromixer is in fluid communication with a dilution fluid connection, a first treatment concentrate connection and a second treatment concentrate connection, and a flow control device, wherein the flow control device includes a flow movable controller that extends along a handpiece assembly of the handpiece, said flow movable controller being configured to be handled and selectively moved by a user of the device; wherein movement of the flow movable controller in a first direction results in an increase in a flowrate of fluids through the at least one delivery conduit, and wherein movement of the flow movable controller in a second direction results in a decrease in the flowrate of fluids through the at least one delivery conduit, said second direction being generally opposite of said first direction; and wherein the micromixer promotes mixing of fluids entering the inlet.

[0049] In some embodiments, the techniques described herein relate to a method of treating a skin of a subject using a device, the method including: connecting a handpiece to a dilution fluid conduit at a dilution fluid port and a vacuum conduit at a vacuum port, wherein the handpiece compromises a distal end and a proximal end, the handpiece including at leastEDGE.222WO -7- Knobbe, Martens, Olson & Bear, LLPone delivery conduit and at least one waste conduit; and placing a concentrated treatment serum vial in fluid communication with the handpiece via a concentrate connector; wherein positioning an interface portion located at the distal end of the handpiece against a skin surface and activating a vacuum source transfers a volume of diluted treatment fluid from a mixing zone to the interface portion, wherein the mixing zone is configured to receive fluid drawn from a dilution fluid connection and the concentrate connector.

[0050] In some embodiments, the techniques described herein relate to a method of treating a skin of a subject using a device, the method including: receiving an at least one dilution fluid via at least one dilution fluid conduit in fluid communication with a mixing zone of the device; receiving at least one container of a concentrated treatment material within a recess or opening of the device; creating a vacuum along a distal end of the device, the distal end of the device being in fluid communication with the mixing zone; mixing the concentrated treatment material and the at least one dilution fluid in the mixing zone into a mixture; and delivering the mixture to the distal end of the device.

[0051] In some embodiments, the techniques described herein relate to a canister configured to store a concentrated treatment material for treating a skin surface, the canister including: an outer wall defining an outer perimeter of the canister, the outer wall including an opening configured to connect to a device for treating a skin surface; and at least one housing wall within the outer wall, the at least one housing wall dividing an internal volume of the canister into at least two divided volumes; wherein the at least two divided volumes are in fluid communication with the opening; and wherein the at least two divided volumes are not in fluid communication with each other.

[0052] In some embodiments, the techniques described herein relate to a canister, wherein the at least one housing wall is configured to deform with the canister such when the canister is deformed, the at least two divided volumes are not put in fluid communication with each other.

[0053] In some embodiments, the techniques described herein relate to a canister, wherein the at least one housing wall is not configured to deform with the canister such when the canister is deformed, the at least one housing wall ruptures or otherwise breaks so the at least two divided volumes are put in fluid communication with each other.EDGE.222WO -8- Knobbe, Martens, Olson & Bear, LLP

[0054] In some embodiments, the techniques described herein relate to a canister, wherein one of the at least two divided volumes contains a first treatment material component and one of the at least two divided volumes contains a second treatment material component; wherein the first treatment material component can react with the second treatment material component to activate the first treatment material component to form an activated treatment material; wherein the activated treatment material can be diluted by a dilution fluid to treat the skin surface.

[0055] In some embodiments, the techniques described herein relate to a canister configured to store a concentrated treatment material for treating a skin surface, the canister including: an outer wall defining an outer perimeter of the canister, the outer wall including an opening configured to connect to a device for treating a skin surface; and a concentrated treatment material configured to be diluted by a dilution fluid connected to the device; wherein the canister is configured such that the concentrated treatment material can be drawn through the opening to the device; and wherein the concentrated treatment material is suitable for use in a skin treatment procedure after mixing with the dilution fluid.

[0056] In some embodiments, the techniques described herein relate to a canister, wherein the outer wall includes one or more rigid or semi-rigid materials.

[0057] In some embodiments, the techniques described herein relate to a canister, wherein the outer wall includes one or more flexible or semi-flexible materials.

[0058] In some embodiments, the techniques described herein relate to a canister, wherein the opening connects to a recess within the device, wherein the recess is configured to receive the canister the outer wall includes one or more flexible or semi-flexible materials.

[0059] In some embodiments, the techniques described herein relate to a canister, wherein the canister is at least partially within the device when the opening is connected to the device.

[0060] In some embodiments, the techniques described herein relate to a canister, wherein the canister is configured such that a vacuum source can draw the concentrated treatment material from the canister into the device.

[0061] In some embodiments, the techniques described herein relate to a canister, wherein the canister is configured such that the concentrated treatment material is provided to the device by gravity feed.EDGE.222WO -9- Knobbe, Martens, Olson & Bear, LLP

[0062] In some embodiments, the techniques described herein relate to a canister, wherein the canister is configured such that the concentrated treatment material is provided to the device by at least partially deforming the canister.

[0063] In some embodiments, the techniques described herein relate to a canister, further including a dilution fluid port located on a first end substantially opposite a second end with the opening, such that a dilution fluid enters the first end of the canister and mixes with the concentrated treatment material to form a mixture; wherein the mixture exits the opening on the second end to the device.

[0064] In some embodiments, the techniques described herein relate to a canister, wherein the opening connects to the device by a mechanical connection.

[0065] In some embodiments, the techniques described herein relate to a canister, wherein the canister is configured to interface with a plurality of protrusion members configured to positively engage the canister,

[0066] In some embodiments, the techniques described herein relate to a canister, wherein connecting the canister to the device produces an auditory click.

[0067] In some embodiments, the techniques described herein relate to a canister, wherein connecting the canister to the device produces a motor-sensory feedback.

[0068] In some embodiments, the techniques described herein relate to a canister, wherein connecting the canister to the device produces a positive engagement notification.

[0069] In some embodiments, the techniques described herein relate to a canister, wherein connecting the canister to the device produces a light, wherein the light can be located on the device.

[0070] In some embodiments, the techniques described herein relate to a canister, wherein the canister can be secured to the device with a locking cap.

[0071] In some embodiments, the techniques described herein relate to a canister, further including a membrane at the opening configured to substantially prevent the concentrated treatment fluid from exiting the canister; wherein the membrane is configured to be pierced by a spike on the device when connecting the canister to the device.EDGE.222WO -10- Knobbe, Martens, Olson & Bear, LLPBrief Description of the Drawings

[0072] These and other features, aspects and advantages of the present application are described with reference to drawings of certain embodiments, which are intended to illustrate, but not to limit, the present inventions. It is to be understood that these drawings are for the purpose of illustrating the various concepts disclosed herein and may not be to scale.

[0073] FIG. 1 A illustrates a perspective view of handpiece configured for use in a skin treatment system, according to one embodiment;

[0074] FIG. IB illustrates a side view of the handpiece of FIG. 1A;

[0075] FIG. 2A illustrates a perspective view of one embodiment of a tip configured to secure along a distal end of a skin treatment handpiece;

[0076] FIG. 2B illustrates a side cross-sectional view of the tip of FIG. 2A;

[0077] FIG. 3A illustrates a perspective view of a handpiece configured for use in a skin treatment system, according to one embodiment;

[0078] FIG. 3B illustrates a cross-sectional perspective view of a handpiece configured for use in a skin treatment system, according to one embodiment;

[0079] FIG. 3C illustrates a cross-sectional perspective view of a handpiece configured for use in a skin treatment system, according to another embodiment;

[0080] FIG. 3D illustrates a side view of internal components of a handpiece configured for use in a skin treatment system, according to another embodiment;

[0081] FIG. 4A illustrates a diagrammatic representation of a cross-sectional view of a concentrate canister for use in a skin treatment system, according to one embodiment;

[0082] FIG. 4B illustrates a cross-sectional perspective view of a handpiece configured for use in a skin treatment system, according to one embodiment;

[0083] FIG. 5 illustrates a cross-sectional perspective view of a handpiece configured for use in a skin treatment system, according to one embodiment; and

[0084] FIG. 6 illustrates a cross-sectional perspective view of a handpiece configured for use in a skin treatment system, according to one embodiment.Detailed Description

[0085] Although the various embodiments of a handpiece and / or other components / portions of a skin treatment system have specific relevance to skin treatment (e.g.,EDGE.222WO -11- Knobbe, Martens, Olson & Bear, LLPdermabrasion, microdermabrasion, skin surfacing / resurfacing, applications of treatment materials on skin, etc.), the features, advantages, and other characteristics disclosed herein may have direct or indirect applicability in other applications, such as, for example, medical devices, mechanical devices (e.g., for medical devices and / or procedures related to skin treatment) and / or the like.

[0086] Several embodiments disclosed herein are particularly advantageous because they include one, several, or all of the following benefits: benefits to the manufacturer of skin serums / other treatment materials, benefits to the users who need to purchase, store and use skin serums / other treatment materials, benefits to the environment and / or the like. For example, the use of concentrated treatment solutions can facilitate the packaging, storage, transportation, use and / or other activities associated with such serums and other treatment materials, such as by reducing the packaging size, transportation costs, transportation emissions, and / or inventory management of the treatment material. As a result of reducing the size of treatment containers (e.g., bottles, vials, other containers, etc.), the amount of waste caused by the production, shipping, use, and / or disposal of the treatment containers is reduced, the cost of manufacturing and shipping is reduced, the amount of space required for storage is reduced and the cost for transporting treatment fluid is reduced and / or the like. In some embodiments, the arrangements disclosed herein provide for enhanced delivery of treatment fluids to the skin of a subject, provide a larger surface area for a skin treatment tip that can help with the treatment of certain anatomical portions (e.g., a person’s back or torso, other larger areas, relatively bony portions, etc.), achieve better exfoliation / extraction and nourishment performance (e.g., using treatment fluids), create more even distribution of serums and / or other treatment fluids / materials to larger coverage areas (e.g., for both exfoliation and extraction at in a faster manner; facilitating a user to exert forces to a skin surface (e.g., especially a “bony” skin surface) and / or the like) and / or provide one or more additional benefits or advantages.

[0087] FIGS. 1A and IB illustrate one embodiment of a handpiece or handheld device 10 configured to be used for a skin treatment system. In some embodiments, the handpiece 10 is part of a larger skin treatment system, such as, for example, a system that includes a tower or console (not shown). Such a tower or console can comprise a manifold assembly for receiving one or more (e.g., 1, 2, 3, 4, more than 4, etc.) bottles or other containers of treatment fluids to be used in a skin treatment procedure. The tower assembly can furtherEDGE.222WO -12- Knobbe, Martens, Olson & Bear, LLPinclude one or more waste canisters or other containers that are configured to receive spent fluids, exfoliated, or otherwise removed skin tissue and / or other waste products resulting from a skin treatment procedure. In some arrangements, one or more of the bottles, canisters or other containers that are designed and otherwise adapted to be secured to the tower assembly can be replaceable, interchangeable and / or otherwise removable (e.g., for emptying, autoclaving or other types of cleaning, replacement, etc.).

[0088] In embodiments of a skin treatment system that include tower assembly or console, such tower assembly or console comprise (and / or can be configured to communicate or work with) one or more input and / or output devices (e.g., a touchscreen or other monitor, a keyboard, other controllers, etc.), an outer housing or other exterior structure, tubing, one or more trays or other storage components, casters, wheels or other moving members or components, interior components (e.g., processor, memory, power source, sensors, tubing, valves and / or other hydraulic components, electrical wiring and other electrical components, etc.) and / or the like. In some embodiments, a tower system or console comprises a vacuum or suction source that is configured to couple to a handpiece 10. In some embodiments, the tower system can be configured to connect the suction source to the handpiece by a vacuum connector 40 positioned on the handpiece.

[0089] A skin treatment system can be configured to permit a user (e.g., an aesthetician, a cosmetician, a dermatologist, etc.) to connect a handpiece 10 to a manifold assembly as noted above (e.g., using a “dummy” cartridge 34, a luer or other connector 30, a fluid conduit extending from the luer 30 to a manifold system of a tower assembly, etc.), and / to insert an actual fluid or treatment material-containing cartridge, such as, e.g., a vial, (not shown) directly into the handpiece 10, e.g., a recess 36 of the handpiece assembly. These two options can be used interchangeably to perform a treatment procedure or protocol, as desired or required.

[0090] Irrespective of if a vacuum or suction source is included as part of the skin treatment system and / or its various components (e.g., tower assembly, handpiece, etc.) or provided separate of the skin treatment system, the handpiece 10 can include one or more vacuum or suction ports or connectors 40. As shown in FIGS. 1A and IB, such a vacuum port or connector 40 can be located along a proximal end 14 of the handpiece 10. As discussed, the vacuum port or connector, and thus the handpiece, can be placed in fluid communication withEDGE.222WO -13- Knobbe, Martens, Olson & Bear, LLPa vacuum or suction source (e.g., a pump), which may or may not be included with and / or be part of a skin treatment system.

[0091] According to some embodiments, once a handpiece 10 is coupled to a vacuum source (e.g., via a vacuum port 40, a vacuum or suction conduit (not shown), etc.) and the vacuum source is activated, the vacuum source generates suction (e.g., a negative pressure relative to atmospheric or ambient pressure) along the distal end 12 of the handpiece 10. In some arrangements, such a suction or vacuum force along the tip or distal end of a handpiece can help draw or deliver one or more treatment fluids to the distal end 12 (e.g., via a manifold assembly of a tower or console (not shown), via a vial or cartridge positioned directly within the handpiece 10, etc.). In some embodiments, the distal end 12 of the handpiece 10 is shaped, sized and / or otherwise configured to receive a tip, such as the tip 100 illustrated in FIGS. 2A to 2B, any other tip disclosed herein and / or equivalents or modifications thereof.

[0092] With further reference to FIGS. 1A and IB, the distal end 12 of the handpiece can include at least one fluid delivery port or opening 22 and / or at least one vacuum port or opening 24. In some embodiments, the fluid delivery port or opening 22 is configured to be placed in fluid communication with a fluid source (e.g., a manifold system and bottles secured thereto, a vial or fluid container positioned directly into a recess or opening 36 of the handpiece 10, etc.). Such fluid communication can be facilitated by one or more conduits, channels or other passages that are internal or external to the handpiece.

[0093] Likewise, in some embodiments, the vacuum port or opening 22 is configured to be placed in fluid communication with a vacuum or suction source (e.g., in a tower or console of a skin treatment system, in the handpiece 10, included in another portion or component of the skin treatments system, separate of the skin treatment system, etc.). Such fluid communication can be facilitated by one or more conduits, channels or other passages and / or fluidic components or features that are internal or external to the handpiece.

[0094] As will be discussed in connection with one or more tip embodiments disclosed herein, the tip can be configured to form a seal (e.g., at least a temporary one, a partial seal, a full seal, etc.) with the skin surface being treated when the tip is positioned in a specific manner relative to such skin surface. For example, the tip can include a peripheral lip or other outer member 132 (see, e.g., FIG. 2A) that is configured to contact skin tissue during use of the handpiece. Such a lip or other peripheral member 132 can be configured to form at least aEDGE.222WO -14- Knobbe, Martens, Olson & Bear, LLPpartial seal with skin and help generate a vacuum or suction force within an interior area circumscribed by the lip or member when the handpiece is coupled to a vacuum source and such vacuum source is activated. In turn, according to some arrangements, the vacuum or suction force along such an interior area along the tip or distal end (e.g., generated by a vacuum or suction source that is placed in fluid communication with the handpiece) can help draw or otherwise transfer fluids and / or other treatment materials from one or more sources to the tip or distal end (e.g., using the suction or vacuum alone without the need for positive pressure exerted on the fluids or other treatment materials being transferred to the tip or distal end). Thus, in such arrangements, treatment fluids and / or other materials from vials, bottles, other containers and / or other fluid source are “pulled” or suctioned or otherwise moved to the handpiece 10 to the distal end 12 (and any tip secured thereto) using suction created along the distal end of the handpiece 10.

[0095] In other embodiments, treatment fluids and / or other materials can be transferred to the handpiece assembly 10 from the manifold assembly using the application of positive pressure within or along the main fluid conduit and / or the bottles or other containers secured to the manifold assembly. This can be accomplished either in lieu of or in addition to the generation of suction along the handpiece assembly 10 (e.g., as discussed herein).

[0096] Fluids and / or other treatment materials from two or more bottles or other containers secured to a manifold assembly or other fluid distribution system can be delivered to the handpiece assembly 10 sequentially or simultaneously, as desired or required. As discussed, the fluid network of the system can include one or more valves, other flow control devices or member, flow meters and / or the like to help regulate the flow of fluids and / or other materials from a fluid source (e.g., a manifold assembly, a vial or cartridge, etc.) to the tip 12 or distal end 14 of a handpiece.

[0097] According to some embodiments, the system is configured to deliver treatment fluid from a fluid source (e.g., a vial or other container secured to the handpiece, one or more bottles coupled to a manifold, etc.) continuously. However, in other embodiments, treatment fluid(s) can be provided using a pulsed pattern and / or non-continuously. In some arrangements, a pulsed pattern can be created by selectively actuating one or more valves and / or other hydraulic features or components of the handpiece and / or any other portion of a skin treatment system. In other embodiments, pulsing is created by selectively changing oneEDGE.222WO -15- Knobbe, Martens, Olson & Bear, LLPor more operational parameters of the suction or vacuum source (e.g., turning a vacuum / suction pump on and / or off, varying the amount of suction that is generated by the vacuum / suction pump or other source, etc.), either in lieu of or in addition to modulating or otherwise manipulating one more valves and / or other fluidic components or features, as desired or required.

[0098] According to some embodiments, the handpiece 10 includes a lever or other controller 50 (e.g., switch, button, roller, other mechanical, electrical and / or electromechanical device or component) for adjusting the flow of treatment fluids and / or materials to the distal end 12 of the handpiece. A tip can be removably secured to the distal end 12 of the handpiece. Additional information regarding one or more embodiments of tips that may be secured to the distal end 12 of a handpiece 10 such tips is provided below.

[0099] The tips illustrated and discussed herein, and / or equivalents thereof, can be configured to secure to one or more other types of handpieces and / or other modalities (e.g., besides the handpiece illustrated in FIGS. 1A and IB herein). For example, the tip can be secured to a larger or smaller handpiece, such as, for example, a lymphatic handpiece, a handpiece intended for body treatments or other larger area treatments and / or the like.

[0100] With continued reference to FIGS. 1 A and IB, a cartridge 34, regardless of whether it is a cartridge that comprises fluid or other treatment material within an interior chamber or whether it is a “dummy” cartridge 34 that places the handpiece in fluid communication with a manifold or other fluid source or network, is configured to be removably positioned within a recess 36 of the handpiece 10. In some embodiments, the recess 36 comprises an open recess that is accessible along the exterior of the handpiece assembly. In some arrangements, the recess 36 is easily accessible from the exterior of the handpiece assembly to permit a user to insert and remove a cartridge 34, at least partially (e.g., partially, fully, etc.), within / out of the recess 36 without manipulating any portion of the handpiece 10 (e.g., without opening an interior of the handpiece, without taking any additional steps, etc.), as desired or required. In some configurations, the recess 36 is located along the proximal end of the handpiece 10 and faces or is oriented in an opposite or substantially opposite direction than the distal end 12 (and any tip secured along the distal end 12) of the handpiece 10.

[0101] For any of the skin treatment system embodiments disclosed herein, a cartridge 34 can be configured to be selectively locked and unlocked to the handpiece 10.EDGE.222WO -16- Knobbe, Martens, Olson & Bear, LLPLocking can include the temporary securement of a cartridge or other container to the handpiece (c.g., such that normal or typical attempts to remove or separate the cartridge or other container from the handpiece will not result in removal of the cartridge from the handpiece). In some arrangements, the cartridge 34 can be locked and unlocked using a rotation, twisting, pushing, sliding and / or other movement of the cartridge relative to the handpiece once the cartridge is secured to the handpiece. According to some embodiments, the cartridge 34 is configured to lock to the main body portion of the handpiece using one or more devices or methods, such as, for example, locking tabs, clasps, magnetic connectors, other fasteners and / or the like.

[0102] As noted above, in some embodiments, the “dummy” cartridge 34 of FIGS. 1A and IB can be replaced with a fluid and / or other treatment material vial or cartridge. Such a vial or other container can include one or more chambers that contain a serum and / or other treatment fluid or material. In such embodiments, the vacuum or suction force generated along the tip or distal end of the handpiece can help draw serums and / or other treatment fluids / materials stored within the vial or other container to the tip or distal end 12 when the tip or distal is positioned along the targeted skin surface.

[0103] In some embodiments, both a “dummy” cartridge 34 that is in fluid communication with a manifold (e.g., of a tower or console system) and a vial or cartridge that itself includes one or more serums or fluids, (e.g., a booster vial, a concentrate or concentrated canister, a concentrated vial, etc.), can together be used in a particular system and / or procedure. Therefore, in some arrangements, the user can swap cartridges and / or other containers 34 during and / or between treatment steps or procedures, as desired or required.

[0104] As noted herein, a handpiece 10 can be used with one or a variety of skin treatment tips that can be removably and / or replaceably positioned along a distal end of handpiece. At least some embodiments of such tips 100 are illustrated herein. See, for example, FIGS. 2A to 2B. A tip can include any tips disclosed in U.S. Patent No. 8,048,089, filed as U.S. Pat. Appl. No. 11,392,348 on March 29, 2006 and issued on November 1, 2011, the entirety of which is hereby incorporated herein and made a part of this specification. See, for example, Figures 5A to HE and the corresponding disclosure, in U.S. Pat. No. 8,048,089. See for example, U.S. Pat. Appl. No. 29 / 904,160, filed August 29, 2023, the entirety of which is hereby incorporated herein and made part of this specification. See for example, U.S. Pat.EDGE.222WO -17- Knobbe, Martens, Olson & Bear, LLPAppl. No. 29 / 904,162, filed August 29, 2023, the entirety of which is hereby incorporated herein and made part of this specification. See for example, U.S. PCT Application PCT / US2024 / 015056, filed February 2, 2024, claiming priority to U.S. Pat. Appl. No. 63 / 444,872 filed February 10, 2023, the entirety of which is hereby incorporated herein and made part of this specification. See for example, U.S. Pat. Appl. No. 29 / 811,070, filed October11, 2021, the entirety of which is hereby incorporated herein and made part of this specification. See for example, U.S. PCT Application PCT / US2023 / 083689, filed December12, 2023, the entirety of which is hereby incorporated herein and made part of this specification. See for example, U.S. PCT Application PCT / US2022 / 077811, filed October 7, 2022, the entirety of which is hereby incorporated herein and made part of this specification. See for example, U.S. Pat. Appl. No 18 / 035,723 filed May 5, 2023, claiming priority to U.S. PCT Application PCT / US2021 / 072294, filed November 8, 2021, claiming priority to U.S. Pat. Appl. No 63 / 111474 filed November 9, 2020, the entirety of which is hereby incorporated herein and made part of this specification.

[0105] FIGS. 2A to 2B illustrate different views of one embodiment of a tip 100 configured to be removably secured to, along, or near a distal end 12 of a handpiece 10. The embodiment depicted in FIGS. 2A to 2B has a generally circular (e.g., circular, substantially circular, etc.) proximal portion 110 and a generally oblong (e.g., oblong, substantially oblong, etc.) distal portion 130. The proximal portion of the tip 100 can be configured to secure (e.g., removably) to a distal end 12 of a handpiece 10. For example, the illustrated tip 100 comprises an oblong portion (e.g., having an oblong shape or a substantially oblong shape) 114 at its distal portion 130, and a circular or cylindrical portion (e.g., having a circular or cylindrical shape or a substantially circular or cylindrical shape) at its proximal portion 110 that is shaped and sized to couple to the distal end 12 of the handpiece 10. However, a tip can comprise a distal portion 130 which includes any other shape or configuration, as desired or required that would be conducive for treating a skin surface. The proximal portion 110 can connect to the distal end 12 of the handpiece 10 via a friction-fit or similar coupling method or technique and can include, at least in some embodiments, one or more O-rings and / or other sealing components or features. However, in other embodiments, the proximal end 110 of the tip 100 can be configured to couple or otherwise secure to the handpiece assembly using one or more other connections methods or technologies, as desired or required.EDGE.222WO -18- Knobbe, Martens, Olson & Bear, LLP

[0106] With continued reference to the tip 100 depicted in FIGS. 2A and 2B, the tip 100 can comprise a stem or other fluid connector 120. Such a stem 120 can extend, at least partially, within a cavity or other interior area or portion defined by the oblong portion 114 of the proximal end 110 of the tip 100. In some embodiments, the stem or other fluid connector 120 extends proximally past the proximal portion 110 and / or other components of the proximal end of the tip. However, in other embodiments, the stem 120 is flush or substantially flush with the proximal end of the oblong portion 114, as desired or required to adequately interface with the distal end 12 of the handpiece 10. Regardless of its exact shape, size and / or other features, the stem 120 can be configured to at least partially penetrate an opening (e.g., the fluid delivery port or opening 22 located along the distal end 112 of the handpiece 10). Thus, in some arrangements, the one or more openings 122 defined and / or formed by the stem 120 can be configured to receive treatment fluids being delivered through the handpiece and to the tip 100 (e.g., to delivery such fluids to the skin surface being treated).

[0107] With reference to FIG. 2B, the tip 100 can include a curved or partially circular transition 118 between the proximal portion 110 and the distal portion 130. However, in other arrangements, the transition can be sharp and / or non-curved or non-circular.

[0108] As depicted in FIGS. 2 A through 2B, the tip 100 can include one or more internal face features 140 (or protrusions) that form a desired pattern along the region defined by the peripheral lip or member 132 along the distal portion 130 of the tip. Such internal face features 140, or protrusions, can provide one or more functions, including, by way of example, (1) abrading or exfoliating skin tissue as the tip 100 is moved relative to skin tissue being treated, (2) creating a desired flow pattern of any treatment fluids being delivered to the tip from a fluid source (e.g., a vial, a manifold system or other fluid distribution component or system, etc.) and / or the like. The tip 100 can include one or more fluid inlet ports 150. Such fluid inlet ports 150 can be located at, along, or near the center of the tip (e.g., in close proximity to the intersection between the major axis 134 and the minor axis 136 of the distal portion 130 of the tip 100). However, in other embodiments, such fluid inlet ports 150 can be located at any other location of the tip, as desired or required. Regardless of the exact quantity, size, location, orientation and / or other details about the fluid delivery port(s) or opening(s), such port(s) or opening(s) can be in fluid communication with one or more openings 122 (e.g., the opening defined and / or created by the stem 120) that extend to or along a proximal portionEDGE.222WO -19- Knobbe, Martens, Olson & Bear, LLP110 of the tip. Thus, once the tip 100 is secured to the distal end 12 of the handpiece 10, such proximal opcning(s) can be placed in fluid communication with one or more fluid delivery ports, opening, conduits, passages, etc. of the handpiece 10.

[0109] Likewise, the tip can include one or more vacuum ports (or suction opening or waste opening) 154 along the distal portion 130 of the tip. As shown in FIGS. 2A to 2B, a tip can include a plurality of vacuum ports 154. In the illustrated embodiment, the tip 100 comprises a total of two vacuum or suction ports or openings 154. However, in other arrangements, the tip 100 can include more or fewer than 2 (e.g., 1, 3, 4, 5, 6, 7, 8, 9, 10, 10 to 15, more than 15, etc.) vacuum or suction ports or openings 154, as desired or required.

[0110] As best illustrated by FIG. 2B, the vacuum or suction ports or openings 154 can be fluidly coupled to each other such that vacuum or suction is applied to the area prescribed by the peripheral lip 132 relatively equally once the vacuum or suction source is activated. The vacuum or suction ports or openings 154 can pass through the surface formed by the area prescribed by the peripheral lip 132 and extend below the distal portion 130 of the tip (e.g., the face or surface that defines the vacuum or suction ports 154) through one or more vacuum or suction port or opening 154 leading to an annular or other waste suction collection region 126. In some arrangements, spent fluids, other debris and / or other waste and / or other materials that are transferred proximally through the vacuum or suction ports or openings 154 can accumulate or be collected in a waste suction collection region 126 (e.g., the annular region defined between an interior of the oblong portion 114 and the stem 120). In some embodiments, such a waste suction collection region 126 is placed in fluid communication with one or more suction or vacuum ports or openings 24 (see, e.g., FIG. 1 A) of the handpiece 10 to help remove any collected materials away from the tip 100 (e.g., toward a waste container using vacuum or suction that is applied to the handpiece).

[0111] As discussed herein, the tip 100 can include one or more internal face features 140 or protrusions that are configured to at least partially abrade tissue when the tip is moved relative to skin (e.g., advanced along the skin surface, advanced along the skin, pressed on the skin, contacting the skin, etc.). In some embodiments, such features have sharp or substantially sharp features to assist with abrading skin tissue. In some arrangements, such features are stationary and are incorporated in the tip (e.g., as part of a unitary structure with the tip). In some embodiments, the abrading structure(s) is / are positioned along an interiorEDGE.222WO -20- Knobbe, Martens, Olson & Bear, LLPdefined by a peripheral lip 132. Tn other embodiments, the peripheral lip 132 comprises the abrading structure, at least in part, cither in addition to or in lieu of any additional (c.g., interior) abrading internal face features and / or other abrasive members 140. Thus, these components could be more suited for dermabrasion, microdermabrasion and / or other abrasive or similar treatment of the skin of a subject.

[0112] For any of the embodiments disclosed herein or equivalents thereof, the tips can be disposable so that they are discarded and / or replaced during or after a treatment procedure. However, in other arrangements, the tips can be configured (e.g., as a result of their material(s), such as, for example, stainless steel, other metals or alloys, other composites, etc., as result of its construction or design, etc.) to be reusable. Thus, in some arrangements, the tips are configured to withstand the temperature variations, chemicals and / or other conditions to which they may be subjected for cleaning, disinfecting, sterilization and / or the like.

[0113] For any embodiments disclosed herein or equivalents thereof, a plane defined by the distal-most portion of the tip (e.g., along the distal end of the distal portion 130) can be angled (e.g., at angle 0) relative to both the longitudinal axis of the tip (and the handpiece to which is secures) and an orthogonal or perpendicular axis of the longitudinal axis through the use of a curved or partially circular transition 118 or otherwise (see, e.g., FIG. 2B). This can provide one or more advantages or benefits to a user who is manipulating a handpiece assembly, including increasing the surface area of such a plane, providing an improved ergonomics, functionality, and comfort features, and / or the like. In some examples, the angle 0 (see, e.g., FIG. 2B) is 10 to 20 degrees (e.g., 10 to 11, 11 to 12, 12 to 13, 13 to 14, 14 to 15, 15 to 16, 16 to 17, 18 to 19, 19 to 20 degrees, angles between the foregoing ranges, etc.). In other embodiments, the angle 0 is less than 10 degrees or greater than 20 degrees, as desired or required.

[0114] The tips 100 can be manufactured as a single member. In other words, a tip 100 can include a unitary or monolithic structure. Such a tip 100 can be manufactured using any suitable manufacturing method, such as, for example and without limitation, injection molding, other molding, thermosetting or forming technology and / or the like. The tips 100 can be rigid or semi-rigid. In some arrangements, the tips comprise one or more plastic materials. However, the tips 100 can include one or more other types of materials, either in addition to or in lieu of plastics, such as, for example, metals or alloys (e.g., stainless steel),EDGE.222WO -21- Knobbe, Martens, Olson & Bear, LLPrubber, other composites, manmade material, natural materials and / or like, as desired or required. As noted, the tips 100 can be disposable or reusable. In some embodiments, the tips are manufactured using recyclable and / or reusable materials and / or using one or more green or environmentally advantageous methods or technologies.

[0115] According to some embodiments, a skin treatment system (e.g., a handpiece 10, a handpiece 200, another component of a skin treatment system, etc.) is configured to be used with a concentrated treatment fluid and / or other material. In some arrangements, a handpiece 200 is configured to receive a container (e.g., vial, canister, bottle, etc.) 210 of concentrated serum and / or other fluid, as depicted in FIGS. 3A to 3D.

[0116] In some embodiments, a handpiece 200 is configured to apply a treatment serum, fluid and / or other material to the skin by diluting a concentrated form of a treatment serum, fluid and / or other material to treat or otherwise contact a skin surface of a patient. As noted above, such configurations can provide one or more benefits or advantages to the treatment system, the user, the environment and / or the like. For example, the use of concentrated treatment solutions can facilitate the packaging, storage, transportation, use and / or other activities associated with such serums and other treatment materials, such as by reducing the packaging size, transportation costs, transportation emissions, and / or inventory management of the treatment material. As a result of reducing the size of treatment containers (e.g., bottles, vials, other containers, etc.), the amount of waste caused by the production, shipping, use, and / or disposal of the treatment containers is reduced, the cost of manufacturing and shipping is reduced, the amount of space required for storage is reduced and the cost for transporting treatment fluid is reduced and / or the like.

[0117] As depicted in FIGS. 3A to 3D, in some embodiments, the handpiece includes a receiving area, zone or recess 205 configured to at least partially receive a concentrate canister or a canister / container of concentrated treatment material 210 (e.g., at or near a concentrate connector 211). In some embodiments, the concentrate canister or container 210 is or includes a container, canister, vial, sealed container, or some other bottle, device or apparatus configured to store a treatment fluid. The handpiece can be configured to draw a volume of at least one treatment fluid located from the concentrate canister 210 (e.g., with a dilution fluid provided to the handpiece via a dilution fluid port 206) to be mixed at a determined ratio to create a diluted treatment fluid for application to the skin of the subjectEDGE.222WO -22- Knobbe, Martens, Olson & Bear, LLP(e.g., diluted treatment fluid that is provided by the handpiece to the skin surface). In some embodiments, the concentrate canister 210 can include one or more rigid or semi-rigid materials (e.g., a substantially rigid material) to prevent, substantially prevent or reduce the likelihood of deformation of the canister during use. In some embodiments, the concentrate canister 210 is at least partially deformable (e.g., partially, substantially or completely deformable, at least partially bendable, flexible, semi-flexible, etc.).

[0118] In some embodiments, a handpiece 200 includes a distal end 202 and a proximal end 204 with a dilution fluid port 206 (e.g., a dilution fluid connection, a dilution fluid receiver, etc.) and / or a waste fluid port 208 (e.g., a waste fluid connection, a waste fluid receiver, vacuum port, vacuum connection). In some embodiments, the dilution fluid port 206 can connect to a dilution conduit or other fluid passage connected to a tower assembly or console responsible for providing dilution fluid. In some embodiments, the waste fluid port 208 can connect to a waste conduit or vacuum conduit connected to a tower assembly or console responsible for storing or retrieving waste fluid. The handpiece 200 can include a recess 205 at, near or along its proximal end 204 configured to accommodate a concentrate connector 211 (e.g., a treatment serum connection), where the recess and concentrate connector 211 are configured to receive or otherwise accommodate connecting a concentrate canister 2fO (e.g., a concentrated treatment serum vial) to the handpiece 200. In some embodiments, the recess 205 can accommodate various different sizes of a concentrate canister 210. In some embodiments, the recess 205 can accommodate a first size of a concentrate canister 210, 310 and a second size of a concentrate canister 210, 310. The concentrate canister 210 could be positioned so that it is partially within the handpiece 200 (e.g., when secured to the handpiece).

[0119] A cross-section of an embodiment of a handpiece 200 shown in FIG. 3B depicts where the concentrate connector 211 could be located. In other embodiments, the connector 211 can be located at any other location, as desired or required. The concentrate connector 211 can be located along the outer perimeter of the housing of the handpiece 200. The concentrate connector 211 can be located within the recess 205 of the handpiece 200. The dilution fluid port 206 can facilitate the transfer of fluid to the handpiece such that, when mixed with fluid from the concentrate canister 210 connected at a concentrate connector 211, a mixed (e.g., diluted, ready-to-use, activated, etc.) fluid can reach a desired concentration or concentration range. Therefore, in such embodiments, a treatment using the mixed fluid couldEDGE.222WO -23- Knobbe, Martens, Olson & Bear, LLPbe performed on the skin of the subject. Treatment serum or other fluid, after being mixed and diluted (e.g., in a mixing zone such as a mixing chamber 240 or micromixer 250 located within the handpiece 200), can be transferred or otherwise transported within the handpiece 200 by a delivery fluid conduit 222 (or delivery conduit) to or near the distal end 202 of the handpiece 200.

[0120] The distal end 202 could further comprise an interface portion where the handpiece 200 is configured to contact the skin of a subject. The interface portion could be integrally formed with the handpiece 200 (e.g., formed together with the rest or at least one portion of the handpiece), could include or be located with (e.g., as part of or a component of) or within a tip such as tip 100 as disclosed herein, could be separate tip, could be another compatible tip for a skin treatment system and / or the like. For simplicity, the handpiece 200 is described herein, according to at least some arrangements, as interfacing with a tip 100, but embodiments using the other alternatives disclosed herein are also viable embodiments.

[0121] In some embodiments, the handpiece 200 can include a waste fluid connection in fluid communication with a vacuum source. The waste fluid conduit could removably attach to the vacuum port or otherwise be placed in fluid communication with a vacuum port of the handpiece 200. A vacuum source fluidly connected to the vacuum port 208 can help draw this mixed treatment fluid provided by the delivery fluid conduit 222 to the tip 100 once the tip contacts and makes a temporary seal against a skin surface or other surface which would facilitate the formation of an enclosed area including the area prescribed by the peripheral lip 132 of the tip 100. In some embodiments, the vacuum source can, in addition to drawing the treatment material from the mixing chamber 240, also draw the contents of the concentrate canister 210 into the mixing zone in the handpiece 200 to be prepared and / or applied to the skin surface. In some embodiments, the vacuum source can facilitate drawing the contents of the concentrate canister 210 into the handpiece 200 without also drawing the treatment material from the mixing chamber 240. When activated, the vacuum source creates a suction or negative pressure (e.g., a vacuum) within the corresponding fluid conduit and / or other hydraulic component to which the vacuum source is fluidly coupled. Accordingly, a vacuum or suction force is created within the enclosed area of the tip or other distal end. Since the fluid conduit is in fluid communication with the enclosed area, treatment fluid (e.g., dilutedEDGE.222WO -24- Knobbe, Martens, Olson & Bear, LLPserum) can be “drawn” or “pulled” to the enclosed area and the tip or distal end, more generally.

[0122] In some embodiments, treatment fluid and / or other material from the concentrate canister 210 can be gravity fed into the handpiece 200 (e.g., by positioning the concentrate connector 211 along the handpiece 200 such that a portion, substantially all, or all of the concentrate canister 210 is positioned above the handpiece 200 when fully connected). In some embodiments, the contents of the concentrate canister 210 can be drawn into the handpiece 200, at least in pail, by or using the vacuum source. In some embodiments, the contents of the concentrate canister 210 can be provided to the handpiece by at least partially deforming, compacting, crushing and / or manipulating the concentrate canister 210 once connected to the concentrate connector 211. In some embodiments, the concentrate canister 210 can further include one or more dilution fluid ports or other fluid connections such that the dilution fluid passes through and mixes with the contents of the concentrate canister 210 before a skin treatment procedure is commenced or performed and the mixed fluid including the dilution fluid and the concentrated treatment material is applied to the skin surface.

[0123] In some embodiments, the application of vacuum or suction from the vacuum source can additionally remove, at least partially, excess fluid or waste from the treatment area. A volume of exfoliated skin tissue, debris, spent fluid and / or other waste can be reduced via one or more waste networks, which, in some arrangements, include a vacuum internal connection 218 (or waste internal connection, or waste fluid conduit). Such a connection 218 can be hydraulically coupled to a waste connector or vacuum port 208 along the handpiece 200. Thus, the combination of dilution fluid (provided by a dilution fluid port 206 connecting to a dilution fluid internal connection 216 which connects to a mixing zone) and a treatment concentrate (provided by a concentrate connector 211 that connects (e.g., fluidly or hydraulically) to a concentrate fluid internal connection 220 which also connects (e.g., fluidly or hydraulically) to the mixing zone) within the mixing zone results in a mixed treatment fluid suitable for use in the treatment of the skin of a subject. The resulting diluted serum or other mixed treatment fluid can be drawn to the distal end 202 of the handpiece 200 (e.g., to or near the tip 100) via the at least one delivery fluid conduit 222 (e.g., with the assistance of a vacuum applied at the vacuum port 208 upon the formation of an at least temporary seal). The treatment fluid can then be applied to the targeted skin surface accordingEDGE.222WO -25- Knobbe, Martens, Olson & Bear, LLPto one or more protocols or other procedures. Exfoliated skin tissue, spent fluid and / or other debris or waste generated during a procedure can be removed from the treatment area of the skin of the subject via a vacuum internal connection 218 and related hydraulic or fluidic components in fluid communication with a vacuum source. Removed material and / or other waste can be transferred through one or more vacuum or waste ports 208 to a manifold (e.g., of a tower or console system) as disclosed herein to facilitate the use of the handpiece 200. The console system can store the removed material in a waste container, which may or may not be located on and / or otherwise may be secured (e.g., removably) to one or more portions of the console. In some embodiments, the waste container is at least partially transparent or translucent. The waste material can be positioned in an area visible to the user of the handpiece 200 during the procedure, which can advantageously be used as an indicator of the correct use of the handpiece 200 and accompanying systems. A handpiece 200 can have one or more delivery fluid conduits 222, as desired or required by a particular design or use.

[0124] The dilution fluid internal connection 216, vacuum internal connection 218, concentrate fluid internal connection 220 (e.g., a treatment concentrate connection) and / or any other liquid, fluid or gas connectors within or associated with the operation of the handpiece 200 can comprise one or more O-rings or other sealing members to prevent, substantially prevent, or reduce the likelihood of undesirable leaks (e.g., between the handpiece 200 and the fluid or gas connector, other components, etc.). The liquid, fluid or gas connectors can be connected to the handpiece 200 in a variety of ways, including but not limited to, a friction or press-fit connection, a threaded connection, a snap connection, another type of mechanical connection and / or any other type of attachment device or method.

[0125] In some embodiments, the concentrate canister 210 is secured to one or more other portions of a handpiece 200 (e.g., using one or more other methods or devices for securing a cartridge or other container 210). For example, the handpiece 200 can include tabs, flanges, other protrusion members and / or any other features or items that help positively engage one or more portions of concentrate canister 210 positioned therein. In some embodiments, delivery of a concentrate canister 210 to a desired depth of the recess or other receiving area of the concentrate canister 210 can produce an audible click, a positive engagement mechanism (e.g., tactile snap), a positive engagement notification, and / or other perceivable confirmation or occurrence. In some embodiments, the feedback can be motor-EDGE.222WO -26- Knobbe, Martens, Olson & Bear, LLPsensory in nature, where the user of the handpiece 200 can feel (e.g., tactically, audibly, etc.) when proper engagement takes place. In some embodiments, the handpiece 200 can further include a light emitting feature that indicates when a proper engagement is present, whether an incomplete seal is present, whether no container is detected, or any other state regarding the connection between the concentrate canister 210 and the handpiece 200. Such features can help notify the user or operator that a concentrate canister 210 has been properly secured within the handpiece 200. In other arrangements, a separate device, such as, a locking cap, strap or other member can be used to ensure that the concentrate canister 210 remains properly secured to the handpiece (e.g., within the recess or other receiving area of the handpiece). This can help ensure that the canister or other container 210 remains (e.g., constantly) in fluid communication with the fluid delivery system of the handpiece (e.g., a spike which could pierce a membrane on the concentrate canister 210) during use.

[0126] In some embodiments, the rate of delivery of mixed treatment fluid by the delivery fluid conduit 222 to the tip 100 can be modified by the use of a flow rate controller 212. Movement of such a controller, which can include a button, tab, roller, lever and / or the like, can adjust the flowrate of mixed treatment fluids and / or materials to the distal end 202 of the handpiece. In some embodiments, the flow rate controller 212 can comprise a flow movable controller configured to be handled by a user. In some embodiments, the flow rate controller 212 can extend through the main body of the handpiece 200. The flow rate controller 212 can extend to, at least partially, an external portion (e.g., outer or exposed surface) of the main body of the handpiece 200 and be movable relative to the main body of the handpiece 200). In some embodiments, the configuration of the handpiece and the controller facilitate the handling of the handpiece by a user or operator during a procedure. The flow rate controller 212 can be configured such that movement of the flow rate controller 212 in a first direction results in an increase in a flowrate of fluids through the delivery fluid conduit 222, and such that movement of the flow rate controller 212 in a second direction generally opposite the first direction results in a decrease in the flowrate of fluids through the delivery fluid conduit 222. Flow rate changes can be linearly or non-linearly related to the relative movement of the controller 212 by the user, as desired or required.

[0127] A handpiece 200 can include one or more other methods of selecting a desired flowrate of fluids and / or other materials therethrough. For example, in someEDGE.222WO -27- Knobbe, Martens, Olson & Bear, LLPembodiments, the handpiece 200 includes one or more dials, knobs, buttons and / or other devices or features for adjusting the flowrate. Such controllers can be graduated so as to permit a user to select a specific flowrate or relative flow setting (e.g., “HIGH,” “MEDIUM,” “LOW,” etc.). In other arrangements, the handpiece 200 comprises a display (e.g., LED, LCD, etc.) that is adapted to provide information regarding a current flowrate or setting. Further, such a display can be configured to permit users to make flowrate adjustments (e.g., touchscreen display). Further, selection devices or features (e.g., knobs, buttons, dials, etc.) and / or displays can be positioned on the handpiece 200. Alternatively, the controllers can be separate of the handpiece. For example, such devices or features can be connected to the assembly through one or more hardwired and / or wireless connections (e.g., cable, Ethernet line, WAN, LAN, cellular connection, Bluetooth connection, a Wi-Fi Network, Bluetooth, Bluetooth Low Energy, DECT, Near Field Communication, IR-Transmission, ZigBee, etc.). The controller can be mechanical and / or electromechanical in nature. In some embodiments, the handpiece 200 can be controlled by operation of a manifold or other fluid distribution system or component (e.g., positioned in and / or included with a tower or console system) operatively connected to one or more control features of the handpiece 200. Such components or features can control, without limitation, a flow rate of the contents of the concentrate canister 210 to the mixing chamber 240, a flow rate of the mixed treatment material to the distal end 202, a suction force (e.g., intensity, modulation, frequency, etc.) along the distal end 202 via the vacuum port 208, a dilution fluid flow rate to the proximal end 204 via the dilution fluid port 206, and / or any other operational parameter(s) and / or feature(s) of the handpiece 200 and / or other portion of the system, as desired or required.

[0128] According to some embodiments, treatment concentrate from the concentrate canister 210 and dilution fluid (e.g., water) from the dilution fluid conduit can be combined in a mixing zone located within the handpiece (e.g., a main body of the handpiece). The mixing zone can include any form or configuration, as required to accomplish a desired mixing of fluids (e.g., to create a diluted or mixed serum or fluid). In some embodiments, such combining and mixing is accomplished while preventing or reducing the likelihood of backflow or retrograde flow (e.g., moderate or severe backflow). The embodiments disclosed in FIGS. 3B to 3C utilize a mixing chamber 240 for their mixing zone. The mixing chamber 240 as used herein includes any chamber, recess, vessel, tank, reservoir, apparatus, otherEDGE.222WO -28- Knobbe, Martens, Olson & Bear, LLPcontainer and / or any other device, apparatus, component and / or the like that has two or more inlets for fluids to enter and one or more outlets for a mixed (e.g., combined, diluted, activated, prepared) treatment fluid to exit.

[0129] In some embodiments, the mixing chamber includes one or more features that encourage the mixing of the contents from the two or more fluid inlets. The features could include, for example and without limitation, smaller aperture(s) or opening(s) on the fluid outlet(s) as compared to the aperture(s) or opening(s) of the fluid inlet(s). Such a configuration can promote and otherwise encourage mixing of the two fluids to exit the mixing chamber 240 at a higher pressure (e.g., relative to what it entered the mixing chamber 240).

[0130] In some embodiments, the features include a configuration that strategically directs the incoming fluids (e.g., the concentrated serum or other treatment fluid or material and water or other dilutant) such that their fluid flow paths at least partially cross or collide. This can promote more uniform mixing of the fluids.

[0131] In one embodiment, the fluids can be directed toward one another in opposing or substantially opposing directions (e.g., in directions offset by 150 to 210 degrees relative to one another), such that their collision at least partially enhances a level of mixing and dilution of the fluids to create a more uniform or homogeneous diluted serum. In some embodiments, the offset angle is 150 to 210 degrees (e.g., 150 to 160, 160 to 170, 170 to 180, 180 to 190, 190 to 200, 200 to 210, 150 to 210, 160 to 200, 170 to 190, 180 to 200, 170 to 210 degrees, values between the foregoing, etc.), as desired or required.

[0132] In one embodiment, fluids (e.g., concentrated serum or other treatment fluid and the water or other dilutant) are directed into the mixing chamber along directions that are not parallel to one another (e.g., non-parallel directions). This can provide for more enhance mixing of fluids.

[0133] Irrespective of the feature chosen to assist in the mixing of the fluids, the mixed fluid could then be drawn (e.g., transferred from the mixing chamber to the tip or distal end of the handpiece via one or more fluid conduits or passages) via the application of a vacuum or suction along the tip or distal end. In some embodiments, multiple features, mixing zones or mixing chambers 240 can be utilized to ensure mixture of the two or more fluids or materials to be delivered to the skin surface as a mixed treatment fluid. The rate of transfer of diluted serum or other diluted treatment material can be determined, at least in part, by one orEDGE.222WO -29- Knobbe, Martens, Olson & Bear, LLPmore factors, such as, for example and without limitation, the strength of the vacuum or suction force, the position of the flow rate controller 212, the viscosity and / or other properties of the diluted serum and / or the like.

[0134] In some embodiments, a micromixer 250 or similar device, component or feature, as depicted in FIG. 3D, can be used in lieu of or in addition to the mixing chamber 240 for the dilution of a concentrated serum or other treatment fluid or material. A micromixer, as used herein, can include any mixing device, apparatus, component and / or feature that utilizes one or more interference surfaces and / or features (e.g., obstructions) to at least partially obstruct a fluid flow to facilitate the mixing of at least two incoming fluids. This can be accomplished by directing the separate fluid and / or other material streams over the interference surfaces in a tortuous fluid path (e.g., at least a partially tortuous path) as the fluid moves from the proximal end 204 to the distal end 202 of the handpiece. The tortuous fluid path formed by the multitude of interference surfaces and / or features can encourage mixing of the fluids (e.g., due to the multiple changes in flow direction due to the fluid paths through or around the interference surfaces and / or features). As such, the type of and number of interference surfaces and / or features (and thus, the tortuous fluid flow that they create) directly relates to the amount of mixing that occurs within the micromixer 250. In some embodiments, the fluid path can include a tortuosity such that the at least two incoming fluids at least partially turn (or otherwise change direction) and at least temporarily move from the distal end 202 to the proximal end 204 before turning again to move from the proximal end 204 to the distal end 202. Therefore, a multitude of designs for types of and number of interference surfaces exist. In some embodiments, the micromixer 250 and / or similar mixing device or component can generate, at least partially, a turbulent (e.g., non-laminar) flow pattern for the fluids passing through he micromixer.

[0135] The interference surfaces or features can include flat (e.g., planar, or substantially planar) surfaces and / or curved surfaces that extend partially across the crosssection of the micromixer (e.g., within the fluid pathway of the micromixer). The interference surfaces and / or features can include a singular spiral or other curved shape that changes its axis of rotation such that the treatment fluid is twisted or “churned” as it progresses through the micromixer 250. In some embodiments, this can generate a multitude of alternating spiral flow paths. In some embodiments, the interference surfaces are configured to at least partiallyEDGE.222WO -30- Knobbe, Martens, Olson & Bear, LLPobstruct substantially all of the cross-section of a micromixer 250 such that fluid flow is forced through only a portion of the cross-section of the micromixer 250. In some embodiments, the interference surfaces and / or features include a plurality of perforated surfaces that extend, at least partially (e.g., partially, completely, substantially completely, etc.) across the crosssection of the micromixer 250 normal to or substantially normal to the axis formed by the proximal-distal directions of the handpiece 200. Thus, in such configurations, the fluid path through the micromixer 250 requires the fluids to pass through the perforations of the perforated surfaces. The perforations could be organized and configured in such a way that the fluids are redirected multiple times (e.g., 2, 3, 4, 5, more than 5, etc.) to make their way through the micromixer 250.

[0136] The interference surfaces can include a plurality of flexible diaphragms that are configured to default to (e.g. be biased into, plastically deform from, etc.) a closed configuration. Such diaphragms or other movable or flexible members can assume an open configured with the application of sufficient force, and allow a fluid or treatment material to pass through the opening (e.g., temporary opening). The flexible diaphragms could be designed in such a way that the conversion from the closed state to the opened state happens multiple times and / or repeatably at certain flow rates. Repeated opening and closing of flexible diaphragms can promote mixing of the fluid streams (e.g., concentrated serum and water or other dilutant) by increasing turbulent flow patterns of the two or more fluids or materials being mixed.

[0137] According to some embodiments, the interference surfaces and / or features comprise a static helix or helical mixer. Such a mixer can comprise a unitary body comprising a series of curved surfaces and / or features that alternate in their direction of curvature (e.g., as the fluids progress from the proximal end to the distal end of the handpiece 200).

[0138] According to some embodiments, the mixing chamber 240 and / or t micromixer 250 used within a handpiece 200 could include any one or more (e.g., a combination of) configurations of systems or methods of mixing as disclosed herein to accomplish the required or desired mixing effect.

[0139] In some embodiments, a balance of dilution fluid to serum or other treatment material concentrate is desired or necessary for adequate, appropriate or optimal use of the disclosed handpieces 200. Such a dilution effect and corresponding dilution ratio canEDGE.222WO -31- Knobbe, Martens, Olson & Bear, LLPbe determined by and / or be based on one or more factors, requirements and / or considerations. In one embodiment, for example, the ratio depends on a restrictor orifice along the dilution fluid connection at or before the dilution fluid port 206. Adjustment of the size of such a restrictor orifice (e.g., using a valve or other flow control device or feature) while keeping the flow rate from the concentrate fluid internal connection 220 constant can help adjust the concentration of the diluted serum or other treatment fluid.

[0140] In some embodiments, the opposite strategy can be implemented, wherein the flowrate of the concentrate fluid is adjusted while the flowrate of the water or other dilutant or dilution fluid is varied (e.g., using a valve or other flow-regulated device of feature).

[0141] In some embodiments, the flow rate of the dilution fluid and / or concentrated serum or other treatment fluid can be fixed during a procedure.

[0142] In other embodiments, flow control adjustments may be performed simultaneously and / or individually on either or both of the concentrated treatment fluid (e.g., serum) and the dilution fluid, as desired or required.

[0143] Another method to control the dilution ratio (e.g., the ratio of the volume of the concentrate canister 210 and the volume of dilution fluid drawn from the dilution fluid port 206) includes using a dilution control device 230 (e.g., a diluted treatment fluid concentration control device, a flow rate controller, a flow control device, etc.) along the handpiece 200, as shown, by way of example, in FIG. 3C. Such a dilution control device 230 could be configured to adjust the flow (and thus, the flowrate) of treatment fluids and / or materials to the mixing zone within the handpiece 200 from the concentrate canister 210. The dilution control device 230 can extend at least partially to an exterior or outer surface or portion of the main body of the handpiece 200 and can be movable relative to the main body of the handpiece 200. In some arrangements, the dilution control device 230 can be easily handled (e.g., grasped) and manipulated by a user or operator (e.g., using a single hand) during a procedure. The dilution control device 230 can be configured such that movement of the dilution control device 230 in a first direction results in an increase in a flowrate of treatment concentrate through the concentrate fluid internal connection 220, and such that movement of the dilution control device 230 in a second direction (e.g., a direction generally opposite the first direction) results in a decrease in the flowrate of treatment concentrate through the concentrate fluid internal connection 220.EDGE.222WO -32- Knobbe, Martens, Olson & Bear, LLP

[0144] Regardless of how mixing and dilution of the concentrated serum or other treatment material occur, in some embodiments, the ratio of dilution fluid to treatment concentrate can be determined or controlled through a feedback loop control system or control unit. In some embodiments, the feedback loop control system comprises one or more sensors (e.g., located within or downstream from the mixing chamber 240). The use of a feedback loop control system can advantageously regulate (e.g., increase, limit or decrease, etc.) the amount or volume of dilution fluid which enters the mixing chamber 240 (e.g., by modifying the restrictor orifice upstream the dilution fluid line). The measurement of the concentration of the treatment fluid could be performed downstream and / or within the mixing chamber 240, as desired or required by a particular control scheme or protocol. Data and / or other information provided by sensor(s) and / or other components included within and / or operatively coupled to the feedback loop control system (e.g., concentration data, concentration data, etc.) can calculate, estimate and / or determine whether the flow of dilution fluid and / or concentrated serum or treatment material should be adjusted. In some embodiments, the processor or control means can receive input from one or more sensors measuring features of the handpiece 200 and related systems, including but not limited to: a flow rate from the dilution fluid source through the dilution fluid port 206, the presence of a concentrate canister 210 connected to the handpiece 200, a flow rate from the concentrate canister 210 to the mixing chamber 240 or micromixer 250, flow rate from the mixing chamber 240 or micromixer 250 onto the skin surface, and / or the strength of a vacuum source connected to the vacuum port 208 on the handpiece 200.

[0145] Any calculations and / or other determinations can be performed within the handpiece and / or external to the handpiece (e.g., by transmitting data via a wire exiting the handpiece 200 or by wireless transmission through remote connection protocols, such as WAN, LAN, cellular connection, Bluetooth connection, a Wi-Fi Network, Bluetooth, Bluetooth Low Energy, DECT, Near Field Communication, IR-Transmission, ZigBee). The feedback control system or unit could regulate the dilution of the treatment fluid by adjusting (e.g., decreasing or limiting, increasing, etc.) the amount of dilution fluid being provided to the mixing zone, by adjusting (e.g., decreasing or limiting, increasing, etc.) the amount of treatment concentrate being provided to the mixing zone, by adjusting (e.g., decreasing or limiting, increasing, etc.) the vacuum pulled by the vacuum source connected to the handpieceEDGE.222WO -33- Knobbe, Martens, Olson & Bear, LLP200 by the vacuum port 208, by other methods known by one skilled in the art and / or combinations thereof. In some embodiments, modifications could be made to the amount of dilution fluid being provided through the dilution fluid port 206 and dilution fluid internal connection 216 such that the mixture is within a tolerance set for the required, desired, acceptable, ideal and / or other targeted presence of concentrate within a completed treatment fluid.

[0146] In some embodiments, a control unit, control system, or processor can be configured to receive inputs from a user of the device to control one or more functions or operations of a handpiece 200 or connected tower system during or for a skin treatment procedure. The control system can include processors, machine-writable memory, and other components as desired or required to sufficiently perform one or more modifications of treatment features during a skin treatment procedure.

[0147] In some embodiments, the control unit can review data retrieved from one or more sensors or sensing elements measuring characteristics of the handpiece 200 and / or skin treatment procedure (e.g., flow rates at various portions of the handpiece 200, the material composition of the mixed treatment fluid after the concentrated treatment material and the dilution fluid are mixed in the mixing zone, etc.) and calculate, based at least partially on the retrieved data, changes to be made to one or more factors that affect the treatment procedure to achieve a desired result. In some embodiments, these calculations can be performed automatically, through the use of a feedback control system.

[0148] According to some embodiments, the ratio of dilution fluid to treatment concentrate is automatically determined and / or accomplished through a feedback loop control system. The feedback loop control system could measure the mixture and adjust a flow rate of dilution fluid according to a process described herein and / or the like. The concentration of a diluted treatment mixture can be measured continuously and / or intermittently, as desired or required. For example, the concentration can be measured / determined after sufficient time has passed for the mixing zone to be populated with a new mixture and have that fluid leave the mixing zone via the delivery fluid conduit 222. In other arrangements, the concentration of the diluted treatment serum or other material is constantly, substantially constantly, frequently, etc. being measured and / or adjustments to the ratio of concentrated serum to dilution fluid are constantly, substantially constantly, frequently, etc. being made (e.g., to achieve and maintainEDGE.222WO -34- Knobbe, Martens, Olson & Bear, LLPa desired concentration for the diluted product). The feedhack loop control scheme or system can be configured to generate a second or subsequent measurement by comparing the concentration of the second mixture with the tolerance set for the required, desired, acceptable and / or other targeted value or range of concentration of a diluted treatment fluid. The feedback loop control system can be configured to utilize any detected parameters (e.g., via one or more sensors, calculations, determinations, etc.) and make modifications (e.g., to the dilution fluid flow rate controller, to a concentrated treatment material flow rate controller, etc.), as needed until the mixture falls within the tolerance set. This can be conducted intermittently (e.g., every few seconds or minutes, according to a moving or fixed time period, etc.) and / or continuously, substantially continuously, frequently, etc.

[0149] In other arrangements, a handpiece 200 is configured to receive or otherwise interface with a concentrate canister or container 3f0 which can include two or more isolated internal chambers. A cross section of one embodiment of a concentrate canister or container 310 with three internal chambers 312, 314, 316 is depicted in FIG 4A. A concentrate canister or container 310 can have its internal volume divided into more or fewer than three divided volumes or chambers, as desired or required (e.g., at least two chambers, at least three chambers, etc.). For example, a concentrate canister 310 can include one, two, four, five, more than five chambers. The internal chambers 312, 314, 316 of the concentrate canister 310 can be separated by housing walls 314 such that the material of each of the internal chambers can be separated, be fluidly distinct, and / or not be in fluid communication with each other before exiting the concentrate canister 210. The concentrate canister or container 310 could be substantially similar to the single-chamber concentrate canister or container 210 discussed herein unless otherwise described. The concentrate canister 310 can be configured to be placed in fluid communication with a handpiece 200 using one or more concentrate connectors 211. Such connector(s) can be configured to place the handpiece’s fluid delivery system in fluid communication with only a single internal chamber 312, 314, 316 of the concentrate container 310 at any one time. In other embodiments, the connector(s) can be configured to place the handpiece’s fluid delivery system in fluid communication with two or more of the concentrate container’s internal chambers 312, 314, 316 at a time, as desired or required.

[0150] In some embodiments, one or more (e.g., some, all, etc.) of the divided volumes or internal chambers 312, 314, 316 of the concentrate canister 310 is configured toEDGE.222WO -35- Knobbe, Martens, Olson & Bear, LLPstore or house a different material to be diluted by the handpiece 200. Such materials (e.g., treatment fluids, other materials, etc.) can vary in type, concentration and / or in any other way, as desired or required by a particular application or use. For example, in some embodiments, the contents of two or more of the divided volumes of the concentrate canister 310 can include the same material in different concentrations. In some embodiments, the contents of two or more of the internal chambers of the concentrate canister 310 can include the same material and / or a different material (e.g., the same material but with one or more additives and / or other ingredients or components). In some embodiments, the internal chambers 312, 314, 316 can be of equal or substantially equal volumes. In some embodiments, the internal chambers 312, 314, 316 can be of different volumes.

[0151] According to some embodiments, the housing walls 314 separating the internal chambers 312, 314, 316 can be made of and / or include a rigid or a substantially rigid material(s). According to some embodiments, the housing walls 314 separating the internal chambers 312, 314, 316 can be made of and / or include one or more flexible or substantially flexible materials. In some embodiments, the contents of the internal chambers 312, 314, 316 can be designed to be mixed, at least partially, within the concentrate canister 310. However, in other embodiments, at least a portion of the contents are configured to mixed outside the concentrate canister, as desired or required. In some embodiments, the different treatment materials stored in the internal chambers 312, 314, 316 may have increased potency or efficacy during a skin treatment procedure if mixed prior (e.g., immediately prior) a skin treatment procedure. In some embodiments, the contents of two or more of the internal chambers 312, 314, 316 may combine to form a special effect (e.g., an effervescent effect), a thermal reaction and / or may react in some other way to change its efficacy (e.g., composition, feel, impact, etc.) prior to use on a skin surface. In some embodiments, the contents of the internal chambers 312, 314, 316 are mixed prior to providing the treatment material to the mixing chamber 240 to be mixed with the dilution fluid. In some embodiments, at least one of the housing walls 314 separating the divided volumes can be configured to rupture or otherwise break before a skin treatment procedure to mix the contents of the two previously divided volumes.

[0152] In some embodiments where the contents of the internal chambers 312, 314, 316 are to be mixed within the concentrate canister 310, the outer wall 318 may be composed of and / or may include a flexible or relatively or substantially flexible material, and the housingEDGE.222WO -36- Knobbe, Martens, Olson & Bear, LLPwalls 314 can be comprised of compromisable (e.g., brittle or relatively brittle material, breakable material, etc.) material, such that the barriers formed between the internal chambers 312, 314, 316 can be broken, ruptured, or compromised (e.g., can be made at least partially permeable upon compression, manipulation, or insertion of the concentrate canister 310 into a handpiece 200). In some embodiments, the housing walls 314 can be configured to deform with the outer wall 318 such that when the concentrate canister 310 is deformed, the internal chambers 312, 314, 316 are not put in fluid communication with each other. In some embodiments, the housing walls 314 are configured to not deform with the outer wall 318 such that when the concentrate canister 310 is deformed, the internal chambers 312, 314, 316 are put in fluid communication with each other.

[0153] According to some embodiments, the ability to control which internal chambers 312, 314, 316 of the concentrate canister or container 310 is connected to a mixing chamber 240 or micromixer 250 of the handpiece 200 is accomplished by having a rotating disk with an opening located within the concentrate connector 211. The opening could be configured such that rotation of the disk or similar movable member within the concentrate connector 211 aligns the opening with one or more of the chambers of the concentrate canister or container 310 at a time, thus placing the mixing chamber 240 in fluid communication with only that or those internal chamber(s). Rotation or movement of the concentrate canister 310 could likewise control what divided volume of the concentrate canister 310 is in fluid communication with the concentrate connector 211 of the handpiece 200. Rotation or movement of the disk or other movable member can be accomplished by use of a dilution selection controller 330 attached to the disk. Such control or movement can be manual (e.g., as desired by the user) and / or automated (e.g., via automatic movement of the disk or other movable member). Selection of just one of the divided volumes or internal chambers 312, 314, 316 of the concentrate canister 310 could be accomplished by other methods known by one skilled in the art. One embodiment of a handpiece configured to use this concentrate canister 310 and dilution selection controller 330 is depicted in FIG. 4B.

[0154] In some embodiments, two or more different concentrate canisters or containers 210 can be used during a skin treatment procedure. For example, a particular procedure may require the contents (e.g., serums, salicylic acid, other acid, cleaners, anti-acne acids and materials, microcapsules, capsules, other time-release products and substances,EDGE.222WO -37- Knobbe, Martens, Olson & Bear, LLPproteins, brightening or lightening agents, peptides, other fluids or substances, etc.) of two or more different concentrate canisters or containers 210. Thus, a user can load and / or unload (e.g., insert, remove, etc.) a combination of concentrate canisters or containers 210 relative to a receiving area of a handpiece 200 during a treatment procedure, either at the same time or sequentially (e.g., one after another).

[0155] FIG. 5 depicts an embodiment of a handpiece 200 configured for use with two separate concentrate canisters or containers 210 - a first concentrate canister or container 210A connected to the handpiece 200 at a first concentrate connector 211A and a second concentrate canister or container 210B connected to the handpiece 200 at a second concentrate connector 21 IB. In some embodiments, both containers 210A, 210B can have their contents drawn into the handpiece 200 by a concentrate fluid internal connection 220 (220A or 220B, respectively) to transmit their contents to a mixing chamber 240 or micromixer 250.

[0156] In any of the embodiments described herein, the concentrate canister or container 210 or 310 can include, without limitation, a standard or non-standard vial, bottle, ampoule and / or any other container, as desired or required. As discussed in greater detail herein, the handpiece 200 can be configured to secure the concentrate canister or container 210 to the concentrate connector 211 during use. In some embodiments, any treatment materials (e.g., serums, salicylic acid, other anti-acne acids and materials, microcapsules, capsules, other time-release products and substances, other fluids and / or other materials contained within the concentrate canister 210) can be drawn or transferred to or near the tip 100 or distal end of the handpiece with the assistance of one or more suction sources (e.g., the vacuum source configured to remove waste materials from the tip 100).

[0157] The concentrate canister or container 210 or 310 can be preloaded with concentrated serums, fluids and / or other treatment materials that will be used in one or more skin treatment procedures using the handpiece 200. As such, the concentrate canister or container 210 could be loaded with any of the following, alone or in combination, and without limitation or exclusion to non-listed materials; desired human growth factors, cytokines, soluble collagen, antioxidants, matrix proteins, serums, salicylic acid, other anti-acne acids and materials, microcapsules, capsules, other time-release products and substances, peptides, amino acids, UVA and / or UVB sunblocks, other sunblocking agents, skin tightening agents, hyaluronic acid (HA), other hydration agents, hair removal or hair growth suppression agents,EDGE.222WO -38- Knobbe, Martens, Olson & Bear, LLPmedicaments and pharmaceuticals, water, saline, other dilutants or dissolvents, vitamins, chemical exfoliation agents, lotions, soothing agents, skin brightening or lightening agents, other acids, anesthetics, medicants, other non-active or active compounds, other fluids or materials, combination or mixtures thereof and / or any other substance.

[0158] In some embodiments, the concentrated material in the concentrate canister 210 or 310 can be activated and / or otherwise have their efficacy increased or modified (e.g., at least partially) after mixing with the dilution fluid (e.g., immediately, after the passage of a certain time period, etc.). For instance, in some embodiments, the material provided by the concentrate canister 210 or 310 can effervesce and / or otherwise react with the dilution fluid once mixed, but remain in a relatively inert form or mode before mixing. Advantageously, this can allow for increased efficacy of treatments using specific treatment materials, in comparison to other treatments which would provide an effervescing container with the hopes that the potency of the effervescence or other beneficial features relating to the mixture were maintained through the course of shipping and storage. Further, this can beneficially allow for effervescent treatment materials and / or other reactive treatment fluids with lower shelf lives to maintain a high level of reactivity and / or efficacy while stored, until mixed with a dilution fluid.

[0159] The concentrate connector 211 can comprise a hollow spike or other piercing member. As shown, the spike can be sized, shaped, positioned and otherwise configured to penetrate a membrane or other portion of the concentrate canister or container 210 when the concentrate canister or container 210 is inserted within a corresponding opening of the handpiece (e.g., interfaced with the concentrate connector 211). In other embodiments, the spike or other member can be adapted to access an interior portion of the concentrate canister or container 210 in one or more other ways. Accordingly, once the concentrate canister 210 has been properly inserted into the handpiece 200, the membrane or other seal preventing or reducing the likelihood of the contents of the concentrate canister or container 210 from prematurely exiting the capsule is compromised. Thus, the contents of the concentrate canister or container 210 can flow into the handpiece 200 and eventually to the tip / distal end-skin surface interface during use.

[0160] In some arrangements, as discussed in greater detail herein, the concentrate canister or container 210 can comprise water, saline, other dilutants or dissolvents and / or otherEDGE.222WO -39- Knobbe, Martens, Olson & Bear, LLPfluids that can be selectively delivered to the tip 100 to dissolve, dilute and / or otherwise come in contact with one or more solids, granular materials, gels, concentrated solution and / or other substances positioned within, on and / or near the tip or other portion of a handpiece.

[0161] For any embodiments of a concentrate canister or container 210 (e.g., bottle, cartridge, vial, etc.), the membrane or other surface or portion configured to be pierced, punctured or otherwise compromised can be re-sealable. In other words, such a membrane can be adapted to re-seal (e.g., close) or substantially re-seal or close the internal contents of the concentrate container 210 when the canister is removed from the handpiece. Therefore, leakage of serums, other fluids and / or other materials contained within a cartridge can be reduced or prevented. In addition, such membrane or other sealing member can help ensure against contamination of the internal contents by preventing or reducing the likelihood of one or more materials located outside the handpiece from entering the cartridge.

[0162] The concentrate canister or container 210 or 310 can comprise an airless pump bottle mechanism, where the internal chamber(s) further comprise a chamber plate at a proximal end of the concentrate canister 210 or 310 that moves along the internal surface of the concentrate canister 210 or 310 to change the volume of the internal chamber(s) as fluid is drawn from the canister. This could reduce or eliminate the likelihood that the vacuum source fails to pull a treatment serum from the internal chamber(s) of the concentrate canister 210 or 310. This could also be reduced or eliminated by the use of a collapsing bag within the internal chamber(s) or other methods known by one skilled in the art.

[0163] In the embodiment of the handpiece 200 and concentrate canister 210 depicted in FIG. 6, the handpiece can be configured to be substantially smaller than other embodiments of the handpiece 200 as disclosed herein, and the concentrate canister 210 can be much larger than other concentrate canisters 210 or 310 disclosed herein. With this embodiment, the handpiece 200 can be sized without needing to include additional material. This can result in a more ergonomic design or configuration for the handpiece 200, as the tip 100 can be manipulated by grasping the system via the concentrate canister 210 or 310, at least partially. A larger concentrate canister 210 could be beneficial by allowing the user or other operator to have more treatment concentrate available for a longer or multiple procedures. Such a configuration can be used with a concentrate canister 310 which has only a single chamber. The larger configuration can also be used for a concentrate canister or container 310EDGE.222WO -40- Knobbe, Martens, Olson & Bear, LLPthat comprises multiple chambers without compromising on the amount of fluid that would be present in each internal chambers 312, 314, 316 for the concentrate canister 310, or for any other reason where it would be beneficial to have a larger container for a skin treatment system.

[0164] As disclosed herein, the handpiece 200 and / or the concentrate containers can be disposable or reusable. In some embodiments, the handpieces and / or the concentrate containers are manufactured using recyclable and / or reusable materials and / or using one or more green or environmentally advantageous methods or technologies.

[0165] If an embodiment of the handpiece 200 is designed to be reusable, the fluid conduits within the handpiece 200 could be purged between uses and / or between applications of different treatment fluids. In such situations, the contents of the dilution fluid internal connection 216, mixing chamber(s) 240, micromixer 250, delivery fluid conduit 222, tip 100 or interface portion, vacuum internal connection 218 and / or any other component or portion that may come in contact with fluids can be purged (e.g., by passing dilution fluid through the handpiece 200 without having a concentrate canister 210 or 310 connected, configuring an attached concentrate canister 210 or 310 to not output any treatment concentrate, etc.). In some circumstances, at least some portions of the concentrate fluid internal connection 220 and / or the mixing chamber 240 or micromixer 250 may be more difficult to purge with this method alone due to the fluid pathways not being in line with the dilution fluid path through the handpiece 200. As such, purging these portions of the handpiece 200 may require one or more additional steps and / or procedures. For example, a flushing canister can be attached (e.g., fluidly) to the concentrate connector 211. The flushing canister can contain dilution fluid, water, or another cleansing agent which could be delivered to the handpiece to remove the remnants of treatment fluid or a treatment serum from the handpiece 200 and / or sanitize the concentrate fluid internal connection 220 and other conduits within the handpiece 200. In some embodiments, the concentrate connector 211 could be configured to interface with the dilution fluid conduit similar to how the dilution fluid port 206 can. In some embodiments, an adaptor is configured to interface with a concentrate connector 211 on one end, while another end of the adaptor can interface with the dilution fluid conduit, similar to the one which connects to dilution fluid port 206. In some embodiments, an empty concentrate canister 210 could be refilled with dilution fluid or other fluid suitable to purge the handpiece 200.EDGE.222WO -41- Knobbe, Martens, Olson & Bear, LLP

[0166] Methods for use of the handpiece 200 are disclosed herein. After being connected to a dilution fluid reservoir and waste chamber with vacuum source at the dilution fluid port 206 and vacuum port 208 respectively, a concentrate canister 210 could be connected to the handpiece 200 at a concentrate connector 211. The tip 100 or interface portion can be positioned along a skin surface such that the tip 100 or interface portion makes an at least temporary seal with the skin surface. Once a vacuum source is activated, under such circumstances, a negative pressure is created within the delivery fluid conduit 222 and the mixing zone, area or region (e.g., mixing chamber 240, micromixer 250, or other viable system for mixing multiple fluids). Forming this seal generates negative pressure once the vacuum source is activated, and draws or directs fluids from the concentrate canister 210 and the dilution fluid conduit to be mixed in the mixing zone. The mixed fluid could then be drawn or directed to the tip 100 or interface surface, to be used on the skin of a subject. The handpiece would then be advanced over the surface of the subject, applying the mixed fluid.

[0167] After the treatment or a portion of the treatment is completed and the handpiece 200 needs to be cleared, the handpiece can be purged by any acceptable method, including but not limited to any methods disclosed herein. The handpiece 200 could then be re-used by attaching another concentrate canister 210 or 310 and performing a procedure as described herein.

[0168] For any of the embodiments disclosed herein, the dilution fluid can include tap water, other types of water (e.g., distilled water, deionized or demineralized water, softened water, other water that has been at least partially treated, etc.) and / or any other dilution fluid, as desired or required. Such water or other dilution fluid can be delivered to the handpiece under pressure (e.g., positive pressure relative to atmospheric pressure). Alternatively, nonpressurized water or fluid can be used. In some embodiments, the dilution water or fluid is connected to a tap. Alternatively, the source of dilution water or fluid can be a reservoir or other container (e.g., positioned adjacent to the handpiece and / or other components of a treatment system).

[0169] In some embodiments, the use of a multi-compartment concentrate container can help replace, at least in certain circumstances, a manifold system to which separate larger bottles arc usually positioned. This can advantageously simplify a skinEDGE.222WO -42- Knobbe, Martens, Olson & Bear, LLPtreatment system configured to deliver two or more different serums or other treatment materials during a procedure.

[0170] Although several embodiments and examples are disclosed herein, the present application extends beyond the specifically disclosed embodiments to other alternative embodiments and / or uses of the inventions and modifications and equivalents thereof. It is also contemplated that various combinations or subcombinations of the specific features and aspects of the embodiments may be made and still fall within the scope of the inventions. Accordingly, it should be understood that various features and aspects of the disclosed embodiments can be combined with or substituted for one another in order to form varying modes of the disclosed inventions. Thus, it is intended that the scope of the present inventions disclosed herein should not be limited by the particular disclosed embodiments described above, but should be determined only by a fair reading of the claims that follow.

[0171] While the inventions are susceptible to various modifications, and alternative forms, specific examples thereof have been shown in the drawings and are herein described in detail. It should be understood, however, that the inventions are not to be limited to the particular forms or methods disclosed, but, to the contrary, the inventions are to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the various embodiments described and the appended claims. Any methods disclosed herein need not be performed in the order recited. The methods summarized above and set forth in further detail below describe certain actions taken by a practitioner; however, it should be understood that they can also include the instruction of those actions by another party. The methods summarized above and set forth in further detail below describe certain actions taken by a user (e.g., a professional in some instances); however, it should be understood that they can also include the instruction of those actions by another party. Thus, actions such as “moving a handpiece” or “delivering a fluid” include “instructing moving a handpiece” and “instructing delivering a fluid.” The ranges disclosed herein also encompass any and all overlap, subranges, and combinations thereof. Language such as “up to,” “at least,” “greater than,” “less than,” “between,” and the like includes the number recited. Numbers proceeded by a term such as “about” or “approximately” include the recited numbers. For example, “about 10 mm” includes “10 mm.” Terms or phrases preceded by a term such as “substantially” include the recited term or phrase. For example, “substantially parallel” includes “parallel.”EDGE.222WO -43- Knobbe, Martens, Olson & Bear, LLP

[0172] Conditional language used herein, such as, among others, “can,” “could,” “might,” “may,” “c.g.,” and the like, unless specifically stated otherwise, or otherwise understood within the context as used, is generally intended to convey that certain embodiments include, while other embodiments do not include, certain features, elements and / or states. Thus, such conditional language is not generally intended to imply that features, elements and / or states are in any way required for one or more embodiments or that one or more embodiments necessarily include these features, elements and / or states.

[0173] Conjunctive language such as the phrase “at least one of X, Y, and Z,” unless specifically stated otherwise, is otherwise understood with the context as used in general to convey that an item, term, etc. may be either X, Y, or Z. Thus, such conjunctive language is not generally intended to imply that certain embodiments require the presence of at least one of X, at least one of Y, and at least one of Z.

[0174] While the above detailed description may have shown, described, and pointed out novel features as applied to various embodiments, it may be understood that various omissions, substitutions, and / or changes in the form and details of any particular embodiment may be made without departing from the spirit of the disclosure. As may be recognized, certain embodiments may be embodied within a form that does not provide all of the features and benefits set forth herein, as some features may be used or practiced separately from others.

[0175] Additionally, features described in connection with one embodiment can be incorporated into another of the disclosed embodiments, even if not expressly discussed herein, and embodiments having the combination of features still fall within the scope of the disclosure. For example, features described above in connection with one embodiment can be used with a different embodiment described herein and the combination still fall within the scope of the disclosure.

[0176] It should be understood that various features and aspects of the disclosed embodiments can be combined with, or substituted for, one another in order to form varying modes of the embodiments of the disclosure. Thus, it is intended that the scope of the disclosure herein should not be limited by the particular embodiments described above. Accordingly, unless otherwise stated, or unless clearly incompatible, each embodiment of this disclosureEDGE.222WO -44- Knobbe, Martens, Olson & Bear, LLPmay comprise, additional to its essential features described herein, one or more features as described herein from each other embodiment disclosed herein.

[0177] Features, materials, characteristics, or groups described in conjunction with a particular aspect, embodiment, or example are to be understood to be applicable to any other aspect, embodiment or example described in this section or elsewhere in this specification unless incompatible therewith. All of the features disclosed in this specification (including any accompanying claims, abstract and drawings), and / or all of the steps of any method or process so disclosed, may be combined in any combination, except combinations where at least some of such features and / or steps are mutually exclusive. The protection is not restricted to the details of any foregoing embodiments. The protection extends to any novel one, or any novel combination, of the features disclosed in this specification (including any accompanying claims, abstract and drawings), or to any novel one, or any novel combination, of the steps of any method or process so disclosed.

[0178] Furthermore, certain features that are described in this disclosure in the context of separate implementations can also be implemented in combination in a single implementation. Conversely, various features that are described in the context of a single implementation can also be implemented in multiple implementations separately or in any suitable subcombination. Moreover, although features may be described above as acting in certain combinations, one or more features from a claimed combination can, in some cases, be excised from the combination, and the combination may be claimed as a subcombination or variation of a subcombination.

[0179] Moreover, while operations may be depicted in the drawings or described in the specification in a particular order, such operations need not be performed in the particular order shown or in sequential order, or that all operations be performed, to achieve desirable results. Other operations that are not depicted or described can be incorporated in the example methods and processes. For example, one or more additional operations can be performed before, after, simultaneously, or between any of the described operations. Further, the operations may be rearranged or reordered in other implementations. Those skilled in the art will appreciate that in some embodiments, the actual steps taken in the processes illustrated and / or disclosed may differ from those shown in the figures. Depending on the embodiment, certain of the steps described above may be removed, others may be added.EDGE.222WO -45- Knobbe, Martens, Olson & Bear, LLP

[0180] Furthermore, the features and attributes of the specific embodiments disclosed above may be combined in different ways to form additional embodiments, all of which fall within the scope of the present disclosure. Also, the separation of various system components in the implementations described above should not be understood as requiring such separation in all implementations, and it should be understood that the described components and systems can generally be integrated together in a single product or packaged into multiple products.

[0181] For purposes of this disclosure, certain aspects, advantages, and novel features are described herein. Not necessarily all such advantages may be achieved in accordance with any particular embodiment. Thus, for example, those skilled in the art will recognize that the disclosure may be embodied or earned out in a manner that achieves one advantage or a group of advantages as taught herein without necessarily achieving other advantages as may be taught or suggested herein.

[0182] Language of degree used herein, such as the terms “approximately,” “about,” “generally,” and “substantially” as used herein represent a value, amount, or characteristic close to the stated value, amount, or characteristic that still performs a desired function or achieves a desired result. For example, the terms “approximately”, “about”, “generally,” and “substantially” may refer to an amount that is within less than 10% of, within less than 5% of, within less than 1% of, within less than 0.1% of, and within less than 0.01% of the stated amount. As another example, in certain embodiments, the terms “generally parallel” and “substantially parallel” refer to a value, amount, or characteristic that departs from exactly parallel by less than or equal to 15 degrees, 10 degrees, 5 degrees, 3 degrees, 1 degree, 0.1 degree, or otherwise.

[0183] The scope of the present disclosure is not intended to be limited by the specific disclosures of preferred embodiments in this section or elsewhere in this specification, and may be defined by claims as presented in this section or elsewhere in this specification or as presented in the future. The language of the claims is to be interpreted broadly based on the language employed in the claims and not limited to the examples described in the present specification or during the prosecution of the application, which examples are to be construed as non-exclusive.EDGE.222WO -46- Knobbe, Martens, Olson & Bear, LLP

[0184] Unless the context clearly requires otherwise, throughout the description and the claims, the words “comprise”, “comprising”, and the like, arc to be construed in an inclusive sense as opposed to an exclusive or exhaustive sense, that is to say, in the sense of “including, but not limited to”.

[0185] Reference to any prior art in this description is not, and should not be taken as, an acknowledgement or any form of suggestion that that prior art forms part of the common general knowledge in the field of endeavor in any country in the world.

[0186] The invention may also be said broadly to consist in the parts, elements and features referred to or indicated in the description of the application, individually or collectively, in any or all combinations of two or more of said parts, elements or features.

[0187] Where, in the foregoing description, reference has been made to integers or components having known equivalents thereof, those integers are herein incorporated as if individually set forth. In addition, where the term “substantially” or any of its variants have been used as a word of approximation adjacent to a numerical value or range, it is intended to provide sufficient flexibility in the adjacent numerical value or range that encompasses standard manufacturing tolerances and / or rounding to the next significant figure, whichever is greater.

[0188] It should be noted that various changes and modifications to the presently preferred embodiments described herein will be apparent to those skilled in the art. Such changes and modifications may be made without departing from the spirit and scope of the invention and without diminishing its attendant advantages. For instance, various components may be repositioned as desired. It is therefore intended that such changes and modifications be included within the scope of the invention. Moreover, not all of the features, aspects and advantages are necessarily required to practice the present invention. Accordingly, the scope of the present invention is intended to be defined only by the claims.EDGE.222WO -47- Knobbe, Martens, Olson & Bear, LLP

Claims

Claims1. A device for treating a skin surface, comprising: a handpiece having a distal end and a proximal end of a handpiece assembly; wherein the handpiece comprises an opening configured to receive a container, the container configured to contain a concentrated treatment material; an interface portion configured to be positioned along the distal end of the handpiece, said interface portion being configured to contact the skin surface; a mixing zone within the handpiece, the mixing zone configured to receive (i) a volume of concentrated treatment material from the container secured to the opening of the handpiece and (ii) a volume of dilution fluid from a dilution fluid conduit; wherein a mixture of the concentrated treatment material and the dilution fluid creates a diluted treatment fluid configured to be delivered to the interface portion during use; a waste fluid connection in fluid communication with the interface portion of the device and a vacuum source, the waste fluid connection configured to removably attach to a waste fluid conduit; a dilution fluid connection in fluid communication with the mixing zone, the dilution fluid connection configured to removably attach to the dilution fluid conduit, and at least one delivery conduit configured to transfer the diluted treatment fluid from the mixing zone to the distal end of the handpiece when the interface portion is positioned along the skin surface and the vacuum source is activated, thus placing the mixing zone in fluid communication with the interface portion.

2. The device of Claim 1, wherein: the mixing zone comprises a plurality of interference surfaces or features, the plurality of interference surfaces or features comprising planar or curved surfaces that create a tortuous fluid path to promote mixing create a spiral flow path; wherein a ratio of the dilution fluid and the concentrated treatment material is determined before a treatment procedure is commenced or performed; andEDGE.222WO -48- Knobbe, Martens, Olson & Bear, LLPwherein the ratio of the dilution fluid and the concentrated treatment material is controlled, at least in part, by a flow control device, component or feature associated with the dilution fluid connection.

3. The device of Claim 1, further comprising: at least one sensor located within or downstream from the mixing zone to measure or estimate a concentration of the diluted treatment fluid located within or exiting the mixing zone and generate concentration data; wherein the concentration data provided by the at least one sensor are used to regulate at least one of a flowrate of the dilution fluid and a flowrate of the concentrated treatment material entering the mixing zone; wherein the at least one sensor is configured to transmit concentration data to a control unit; and a feedback loop control system, wherein the feedback loop control system receives data regarding the concentration of the diluted treatment fluid; and wherein the feedback loop control system modifies an amount of the dilution fluid and / or concentrated treatment material provided to the mixing zone.

4. The device of Claim 1, further comprising a flow control device, wherein the flow control device is configured to be handled and selectively moved by a user of the device, and wherein the flow control device regulates a flowrate of diluted treatment fluid from the mixing zone to the distal end of the handpiece.

5. The device of Claim 1, further comprising a diluted treatment fluid concentration control device, wherein the diluted treatment fluid concentration control device is configured to be handled and selectively moved by a user of the device, and wherein the diluted treatment fluid concentration control device regulates at least one of a flowrate of the concentrated treatment material to the mixing zone and a flow rate of dilution fluid to the mixing zone.

6. The device of Claim 1, wherein the mixing zone comprises a micromixer or other features that create a tortuous fluid path to promote mixing.

7. The device of Claim 5, wherein the mixing zone comprises a plurality of interference surfaces or features.EDGE.222WO -49- Knobbe, Martens, Olson & Bear, LLP8. The device of Claim 7, wherein the plurality of interference surfaces or features compromise planar or curved surfaces.

9. The device of Claim 6, wherein the micromixer or other features that create the tortuous fluid path to promote mixing create a spiral flow path.

10. The device of Claim 6, wherein the micromixer or other features that create the tortuous fluid path to promote mixing includes perforated surfaces crossing the mixing zone.

11. The device of Claim 6, wherein the micromixer or other features that create the tortuous fluid path to promote mixing includes at least one flexible diaphragms configured to move between at least two positions to promote mixing.

12. The device of Claim 1 , wherein a ratio of the dilution fluid and the concentrated treatment material is determined before a treatment procedure is commenced or performed.

13. The device of Claim 12, wherein the ratio of the dilution fluid and the concentrated treatment material is controlled, at least in part, by a flow control device, component or feature associated with the dilution fluid connection.

14. The device of Claim 12, wherein the ratio of the dilution fluid and the concentrated treatment material is manually controlled.

15. The device of Claim 12, wherein the ratio of the dilution fluid and the concentrated treatment material is automatically controlled.

16. The device of Claim 12, wherein the ratio of the dilution fluid and the concentrated treatment material is controlled, at least in part, using a feedback loop control system.

17. The device of Claim 16, wherein the feedback loop control system comprises at least one sensor, the at least one sensor being configured to detect or approximate a concentration of the diluted treatment fluid exiting the mixing zone.

18. The device of Claim 1, wherein the mixing zone comprises a mixing chamber.

19. The device of Claim 18, wherein the concentrated treatment material and the dilution fluid are mixed by being directed in non-parallel directions when entering the mixing chamber.

20. The device of Claim 1, wherein the mixing zone further comprises a second treatment concentrate connection for an additional concentrated treatment .

21. The device of Claim 1, wherein the device is configured for microdermabrasion.EDGE.222WO -50- Knobbe, Martens, Olson & Bear, LLP22. The device of Claim 1 , further comprising at least one sensor located within or downstream from the mixing zone to measure or estimate a concentration of the diluted treatment fluid located within or exiting the mixing zone and generate concentration data.

23. The device of Claim 22, wherein the concentration data provided by the at least one sensor are used to regulate at least one of a flowrate of the dilution fluid and a flowrate of the concentrated treatment material entering the mixing zone.

24. The device of Claim 22, wherein the at least one sensor is configured to transmit concentration data to a control unit.

25. The device of Claim 24, wherein the control unit is included within the handpiece.

26. The device of Claim 24, wherein the control unit is external to the handpiece.

27. The device of Claim 22, further comprising a feedback loop control system, wherein the feedback loop control system receives data regarding the concentration of the diluted treatment fluid; and wherein the feedback loop control system modifies an amount of the dilution fluid and / or concentrated treatment material provided to the mixing zone.

28. The device of Claim 1, wherein the mixing zone is configured to promote mixing of the dilution fluid and the concentrated treatment material.

29. The device of Claim 1, wherein the interface portion comprises a tip.

30. The device of Claim 29, wherein the tip is removable from the handpiece.

31. The device of Claim 1, wherein the interface portion is removable from the device.

32. The device of Claim 1, further comprising the dilution fluid conduit.

33. The device of Claim 1, further comprising the waste fluid conduit configured to generate a vacuum from the vacuum source.

34. The device of Claim 33, wherein the container comprises a single chamber.

35. The device of Claim 33, wherein the container comprises at least two chambers, each of said at least two chambers being fluidly distinct of each other.

36. The device of Claim 35, wherein the container comprises at least three chambers.EDGE.222WO -51- Knobbe, Martens, Olson & Bear, LLP37. The device of Claim 1 , wherein at least one of the concentrated treatment materials react with the dilution fluid to effervesce.

38. The device of Claim 1, wherein at least one of the concentrated treatment materials react with the dilution fluid to increase its efficacy during a treatment procedure39. A device for treating a skin surface, comprising: a handpiece having a distal end and a proximal end, said handpiece comprising at least one delivery conduit and at least one waste conduit; a tip configured to be positioned along the distal end of the handpiece, said tip being configured to contact the skin surface; a micromixer comprising an inlet and an outlet, the outlet being fluidly connected to the at least one delivery conduit, wherein the inlet of the micromixer is in fluid communication with a dilution fluid connection, a first treatment concentrate connection and a second treatment concentrate connection, and a flow control device, wherein the flow control device comprises a flow movable controller that extends along a handpiece assembly of the handpiece, said flow movable controller being configured to be handled and selectively moved by a user of the device; wherein movement of the flow movable controller in a first direction results in an increase in a flowrate of fluids through the at least one delivery conduit, and wherein movement of the flow movable controller in a second direction results in a decrease in the flowrate of fluids through the at least one delivery conduit, said second direction being generally opposite of said first direction; and wherein the micromixer promotes mixing of fluids entering the inlet.

40. A method of treating a skin of a subject using a device, the method comprising: connecting a handpiece to a dilution fluid conduit at a dilution fluid port and a vacuum conduit at a vacuum port, wherein the handpiece compromises a distal end and a proximal end, the handpiece comprising at least one del ivory conduit and at least one waste conduit; and placing a concentrated treatment serum vial in fluid communication with the handpiece via a concentrate connector;EDGE.222WO -52- Knobbe, Martens, Olson & Bear, LLPwherein positioning an interface portion located at the distal end of the handpiece against a skin surface and activating a vacuum source transfers a volume of diluted treatment fluid from a mixing zone to the interface portion, wherein the mixing zone is configured to receive fluid drawn from a dilution fluid connection and the concentrate connector.

41. A method of treating a skin of a subject using a device, the method comprising: receiving an at least one dilution fluid via at least one dilution fluid conduit in fluid communication with a mixing zone of the device; receiving at least one container of a concentrated treatment material within a recess or opening of the device; creating a vacuum along a distal end of the device, the distal end of the device being in fluid communication with the mixing zone; mixing the concentrated treatment material and the at least one dilution fluid in the mixing zone into a mixture; and delivering the mixture to the distal end of the device.

42. A canister configured to store a concentrated treatment material for treating a skin surface, the canister comprising: an outer wall defining an outer perimeter of the canister, the outer wall comprising an opening configured to connect to a device for treating a skin surface; and at least one housing wall within the outer wall, the at least one housing wall dividing an internal volume of the canister into at least two divided volumes; wherein the at least two divided volumes are in fluid communication with the opening; and wherein the at least two divided volumes are not in fluid communication with each other.

43. The canister of Claim 42, wherein the at least one housing wall is configured to deform with the canister such when the canister is deformed, the at least two divided volumes are not put in fluid communication with each other.

44. The canister of Claim 42, wherein the at least one housing wall is not configured to deform with the canister such when the canister is deformed, the at least one housing wallEDGE.222WO -53- Knobbe, Martens, Olson & Bear, LLPruptures or otherwise breaks so the at least two divided volumes are put in fluid communication with each other.

45. The canister of Claim 42, wherein one of the at least two divided volumes contains a first treatment material component and one of the at least two divided volumes contains a second treatment material component; wherein the first treatment material component can react with the second treatment material component to activate the first treatment material component to form an activated treatment material; wherein the activated treatment material can be diluted by a dilution fluid to treat the skin surface.

46. A canister configured to store a concentrated treatment material for treating a skin surface, the canister comprising: an outer wall defining an outer perimeter of the canister, the outer wall comprising an opening configured to connect to a device for treating a skin surface; and a concentrated treatment material configured to be diluted by a dilution fluid connected to the device; wherein the canister is configured such that the concentrated treatment material can be drawn through the opening to the device; and wherein the concentrated treatment material is suitable for use in a skin treatment procedure after mixing with the dilution fluid.

47. The canister of Claim 46, wherein the outer wall comprises one or more rigid or semi-rigid materials.

48. The canister of Claim 46, wherein the outer wall comprises one or more flexible or semi-flexible materials.

49. The canister of Claim 46, wherein the opening connects to a recess within the device, wherein the recess is configured to receive the canister the outer wall comprises one or more flexible or semi-flexible materials.

50. The canister of Claim 46, wherein the canister is at least partially within the device when the opening is connected to the device.EDGE.222WO -54- Knobbe, Martens, Olson & Bear, LLP51. The canister of Claim 46, wherein the canister is configured such that a vacuum source can draw the concentrated treatment material from the canister into the device.

52. The canister of Claim 46, wherein the canister is configured such that the concentrated treatment material is provided to the device by gravity feed.

53. The canister of Claim 46, wherein the canister is configured such that the concentrated treatment material is provided to the device by at least partially deforming the canister.

54. The canister of Claim 46, further comprising a dilution fluid port located on a first end substantially opposite a second end with the opening, such that a dilution fluid enters the first end of the canister and mixes with the concentrated treatment material to form a mixture; wherein the mixture exits the opening on the second end to the device.

55. The canister of Claim 46, wherein the opening connects to the device by a mechanical connection.

56. The canister of Claim 46, wherein the canister is configured to interface with a plurality of protrusion members configured to positively engage the canister,57. The canister of Claim 46, wherein connecting the canister to the device produces an audible click.

58. The canister of Claim 46, wherein connecting the canister to the device produces motor-sensory feedback.

59. The canister of Claim 46, wherein connecting the canister to the device produces a positive engagement notification.

60. The canister of Claim 46, wherein connecting the canister to the device produces a light, wherein the light can be located on the device.

61. The canister of Claim 46, wherein the canister can be secured to the device with a locking cap.

62. The canister of Claim 46, further comprising a membrane at the opening configured to substantially prevent the concentrated treatment fluid from exiting the canister; wherein the membrane is configured to be pierced by a spike on the device when connecting the canister to the device.EDGE.222WO -55- Knobbe, Martens, Olson & Bear, LLP

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