Sequential electroporation method
JP2024515997A5Inactive Publication Date: 2025-03-27MAXCYTE INC
0 Cites 0 Cited by
Patent Information
- Application Number
- JP2023566751
- Authority / Receiving Office
- JP · JP
- Patent Type
- Applications
- Current Assignee / Owner
- Priority Date
- 2021-04-29
- Filing Date
- 2022-04-27
- Publication Date
- 2025-03-27
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
Technical Problem
Existing electroporation methods face challenges in efficiently introducing agents into difficult-to-transfect cells like immature or primary cells without causing damage or altering their physiological state, due to high energy consumption leading to heat generation and conductivity changes.
Method used
A method involving sequential electroporation with varying electric field strengths and pulse durations, allowing for controlled introduction of agents into cells while maintaining viability and efficiency.
Benefits of technology
This approach significantly enhances transgene expression and maintains cell viability, achieving higher loading efficiency and clinical efficacy by optimizing electroporation energy use.
✦ Generated by Eureka AI based on patent content.
Abstract
Aspects of the present disclosure are directed to techniques for sequential electroporation that provide for the delivery of multiple, time-spaced electrical pulses to cells, cell particles, lipid vesicles, liposomes to increase the efficiency of entry of one or more agents of interest into the cells, cell particles, lipid vesicles, liposomes, tissues, or derivatives thereof, minimize damage from electrical arcs or heat shock, increase the loading efficiency of the agents of interest, and maintain the viability of the cells, cell particles, lipid vesicles, or tissues and the ability of the cells, cell particles, lipid vesicles, liposomes, or tissues to provide a clinical effect. TIFF2024515997000040.tif101170
Need to check novelty before this filing date? Find Prior Art