Biofabrication of aligned tissues induced by light projection

JP2025523581A5Pending Publication Date: 2026-06-22ETH ZURICH
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Patent Information

Authority / Receiving Office
JP · JP
Patent Type
Applications
Current Assignee / Owner
ETH ZURICH
Filing Date
2023-06-28
Publication Date
2026-06-22

AI Technical Summary

Technical Problem

Existing methods for fabricating anisotropic tissues like muscle and tendon lack efficient cell alignment and topographical cues, as they focus on macroscale features that do not provide sufficient guidance for cell arrangement and nuclear deformation, which are crucial for tissue physiology and differentiation.

Method used

A method involving spatially coherent light projection to generate microbeams and voids in a photocrosslinkable hydrogel composition, creating ultra-high aspect ratio structures that align cells and induce deformation, mimicking natural tissue organization.

Benefits of technology

Facilitates rapid and safe encapsulation of cells in a hydrogel matrix with aligned microstructures, promoting efficient cell induction and migration, and enabling the fabrication of complex tissue constructs that mimic natural tissues.

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Abstract

One aspect of the present invention relates to a method for manufacturing a three-dimensional hydrogel bioimplant characterized by a geometric structure in a size range of less than a millimeter. This method includes providing a first composition that is prone to photocrosslinking within a container. This first composition includes a first polymer that is prone to photocrosslinking, a photoinitiator, and optionally a refractive index matching agent, a light-absorbing dye, and a crosslinking agent. In a first irradiation step, the composition is irradiated with a plurality of spatially coherent light rays, thereby generating a plurality of microcolumns within the composition. Another aspect of the present invention relates to a three-dimensional hydrogel implant comprising, or consisting essentially of, a plurality of microbeams of a photocrosslinked polymer, wherein the three-dimensional hydrogel implant is obtained by the method according to the present invention.
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