Composition containing collagen peptides

Abstract

To provide a composition containing collagen peptides that can absorb ultraviolet light and emit visible light. [Solution] A composition comprising (A) hydrolyzed collagen with a molecular weight of 500 to 8000, (B) monosaccharides or oligosaccharides with a degree of polymerization of 2 to 4, and (C) transition metal ions.

Description

Technical Field

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[0001] The present disclosure relates to a composition containing a collagen peptide.

Background Art

[0002] It has long been reported that ultraviolet irradiation of dermal collagen, glue, and gelatin emits fluorescence, and most of it is due to tyrosine residues (Non-Patent Document 1). It has also been suggested that there are some other structural origins (such as binding to sugars).

[0003] Atelocollagen and hydrolyzed collagen used in many cosmetics have decomposed and removed telopeptides with high antigenicity (rich in tyrosine). Due to hydrolysis to a molecular weight of 8000 or less by enzymatic treatment such as pepsin and a large change in structure, the fluorescence intensity in the visible light region is not very large. Regarding the increase in visible fluorescence intensity, there is a case where the addition of alum (potassium aluminum sulfate) to gelatin causes the fluorescence peak to shift slightly to the longer wavelength side and the intensity to increase (Non-Patent Document 2).

Prior Art Documents

Non-Patent Documents

[0004]

Non-Patent Document 1

Non-Patent Document 2

Summary of the Invention

Problems to be Solved by the Invention

[0005] In recent years, attempts have been made to convert harmful light, particularly ultraviolet light, into useful light in the visible range using nonlinear materials such as zinc oxide, spirulina, and red fluorescent materials. However, many of these materials are dispersed in a solid state, limiting their applications.

[0006] If a moisturizing and solubilizable material, such as collagen peptides, can be given the added function of converting ultraviolet light, it could broaden the range of cosmetic design possibilities. The problem that this disclosure aims to solve is to provide a composition containing collagen peptides that can absorb ultraviolet light and emit visible light. [Means for solving the problem]

[0007] This disclosure includes, for example, the following subjects:

[0008] Section 1. A composition comprising (A) hydrolyzed collagen with a molecular weight of 500 to 8000, (B) monosaccharides or oligosaccharides with a degree of polymerization of 2 to 4, and (C) transition metal ions.

[0009] Section 2. The composition according to item 1, which is a composition for converting absorbed near-ultraviolet light into visible light.

[0010] Section 3. The composition according to item 1, which is a composition for absorbing near-ultraviolet light and emitting blue light.

[0011] Section 4. A composition described in any one of items 1 to 3, which is a cosmetic composition or a quasi-drug.

[0012] Section 5. (B) A composition according to any one of items 1 to 3, wherein the monosaccharide contains xylose.

[0013] Section 6. (C) A composition according to any one of items 1 to 3, comprising a transition metal ion, a zinc ion. [Effects of the Invention]

[0014] According to the present invention, it is possible to provide a composition containing a collagen peptide that can absorb near-ultraviolet rays and emit visible light.

Brief Description of the Drawings

[0015] [Figure 1] Three-dimensional fluorescence spectra of each collagen solution. (A) Aqueous solution of collagen peptide alone, (B) Aqueous solution with sugar and alum added to collagen peptide, (C) Aqueous solution with sugar and zinc chloride added to collagen peptide. [Figure 2] Fluorescence spectra of collagen solutions at various excitation wavelengths. Vertical axis: Fluorescence intensity, horizontal axis: Excitation wavelength. (A) Collagen peptide alone, (B) Composition with sugar and alum added to collagen peptide, (C) Composition with sugar and zinc chloride added to collagen peptide. [Figure 3] Photographs of formulation examples (left) Beauty liquid containing collagen peptide composite solution, (right) Control solution not containing collagen peptide composite solution.

Modes for Carrying Out the Invention

[0016] In this specification, "containing" is a concept that also includes "consisting essentially of" and "consisting of only".

[0017] In the numerical ranges described step by step in this specification, the upper limit value or lower limit value of a certain numerical range can be arbitrarily combined with the upper limit value or lower limit value of another numerical range. Also, in the numerical ranges described in this specification, the upper limit value or lower limit value of the numerical range may be replaced with the value shown in the examples or a value uniquely derivable from the examples. Furthermore, in this specification, the numerical values connected by "~" mean a numerical range including the numerical values before and after "~" as the lower limit value and the upper limit value.

[0018] In this specification, "transition metal" refers to an element existing between Group 3 elements and Group 12 elements in the periodic table.

[0019] In this specification, the "near ultraviolet region" refers to the wavelength range from 200 nm to less than 380 nm, and the "visible region" refers to the wavelength range from 380 nm to 780 nm.

[0020] The present disclosure provides a composition comprising (A) hydrolyzed collagen having a molecular weight of 500 to 8000, (B) monosaccharides, and (C) transition metal ions.

[0021] The inventors investigated whether the fluorescence in the ultraviolet region emitted by hydrolyzed collagen peptides irradiated with ultraviolet light could be shifted to the visible region or further enhanced by the addition of additional substances. Unexpectedly, they found that it could be controlled by the addition of monosaccharides and transition metals.

[0022] The applicant of the present application does not intend for the present invention to be bound by specific hypotheses or theories. However, it is considered that when monosaccharides coexist with hydrolyzed collagen peptides capable of absorbing ultraviolet light, a Maillard reaction occurs between some of the hydrolyzed collagen peptides and the monosaccharides, resulting in a shift to longer wavelengths and an increase in the emission in the visible region, which is further enhanced by the presence of transition metal ions. Even if a composition is produced without completely following this hypothesis, as long as it satisfies the requirements defined in the present invention, it is included in the technical scope of the present invention.

[0023] (A) Hydrolyzed collagen with a molecular weight of 500 to 8000 is collagen obtained by hydrolyzing animal-derived collagen with an acid, base, etc. to reduce its molecular weight. (A) Hydrolyzed collagen with a molecular weight of 500 to 8000 may also be called collagen peptide. In some preferred embodiments, the molecular weight of hydrolyzed collagen with a molecular weight of 500 to 8000 is 1000 to 5000. In some preferred embodiments, the molecular weight of hydrolyzed collagen with a molecular weight of 500 to 8000 is 2000 to 4000. The raw material collagen may be collagen derived from fish (skin, scales, etc.), collagen derived from mammals such as cows and pigs (skin, etc.), or collagen derived from other sources (chicken, etc.). The obtained hydrolyzed collagen may be used in solution form, but may be concentrated and used as a concentrated liquid if necessary, or the liquid may be completely evaporated and used as a solid.

[0024] The composition of this disclosure may contain (A) hydrolyzed collagen with a molecular weight of 500 to 8000, which may be one or more types or two or more types.

[0025] The amount of (A) hydrolyzed collagen with a molecular weight of 500 to 8000 in the composition is not particularly limited, but is for example 0.1 to 30% by mass. If the molecular weight is less than 500, it will have high hygroscopicity and a sticky texture, which is undesirable. If the hydrolyzed collagen has a molecular weight of 8000 or less, it will have high moisturizing properties and a good texture.

[0026] (A) For hydrolyzed collagen with a molecular weight of 500 to 8000, the molecular weight stated in the product description, such as in the catalog, may be used in the case of commercially available products. (A) If the molecular weight of hydrolyzed collagen with a molecular weight of 500 to 8000 is not specified, it can be measured by a measurement method using chromatography, particularly the weight-average molecular weight measurement method for collagen peptides (GMJ method) or a measurement method for weight-average molecular weight or number-average molecular weight (polystyrene equivalent) using gel permeation chromatography (GPC), with the GMJ method being preferred.

[0027] (B) Monosaccharides or oligosaccharides with a degree of polymerization of 2 to 4 may be pentasaccharides or hexasaccharides. Examples of pentasaccharides include pentoses and ketopentoses, examples of pentoses include xylose, arabinose, ribose, and lyxose, and examples of ketopentoses include ribulose and xylulose. The (B) monosaccharides contained in the composition of this disclosure may be one type or two or more types. Xylose is particularly preferred in that it effectively converts absorbed ultraviolet light into visible light.

[0028] The amount of (B) monosaccharides or oligosaccharides in the composition is not particularly limited, but is, for example, 0.1 to 5.0% by mass.

[0029] (C) Examples of transition metal ions include, but are not limited to, transition metal cations such as titanium (Ti), manganese (Mn), iron (Fe), ruthenium (Ru), osmium (Os), cobalt (Co), rhodium (Rh), iridium (Ir), nickel (Ni), palladium (Pd), platinum (Pt), copper (Cu), silver (Ag), gold (Au), zinc (Zn), and cadmium (Cd). The (C) transition metal ions contained in the compositions of this disclosure may be one type or two or more types. In terms of effectively converting absorbed ultraviolet light into visible light, the transition metal ions preferably include at least one selected from the group consisting of copper ions, iron ions, and zinc ions, and more preferably include zinc ions.

[0030] (C) Transition metal ions can be produced by incorporating a compound containing a transition metal, particularly a transition metal salt, as a component of the composition. The transition metal salt may be an organic salt or an inorganic salt. In some embodiments, the transition metal salt is an inorganic salt and is a halide, nitrate, sulfate, carbonate, or phosphate. In some embodiments, the transition metal salt is an organic salt and is an acetate or citrate. The compound containing the transition metal may be a hydrate.

[0031] The amount of (C) transition metal ions in the composition is not particularly limited, but in the state of the transition metal-containing compound incorporated as a component of the composition, for example, preferably 0.00001 to 5.0% by mass, more preferably 0.0001 to 5.0% by mass, more preferably 0.001 to 5.0% by weight, and even more preferably 0.01 to 5.0% by mass. The upper limit of the amount of (C) transition metal ions in the composition may be 1.0% by mass.

[0032] The compositions of this disclosure may, if necessary, contain ingredients commonly used in the formulation of cosmetics, quasi-drugs, pharmaceuticals, etc., to the extent that they do not impair the effects of the present invention. Examples of such ingredients include water, oils, surfactants, metal soaps, thickeners, powders, alcohols, water-soluble polymers, film-forming agents, resins, UV protection agents, inclusion compounds, antibacterial agents, fragrances, deodorants, salts, pH adjusters, cooling agents, animal or microorganism-derived extracts, plant extracts, blood circulation promoters, astringents, anti-seborrheic agents, whitening agents, anti-inflammatory agents, free radical scavengers, cell activators, humectants (glycerin, dipropylene glycol, etc.), chelating agents, keratolytic agents, enzymes, hormones, vitamins, etc. One or more of these ingredients may be incorporated into the compositions of this disclosure.

[0033] To prepare the compositions of this disclosure, for example, the components of the preceding compositions may be mixed. The compositions of this disclosure typically contain water and / or a hydrophilic solvent such as ethanol, and are used as a solution or dispersion of water and / or a hydrophilic solvent.

[0034] The compositions of this disclosure may be provided in the form of aqueous solutions or in the form of emulsified compositions (e.g., oil-in-water compositions).

[0035] The compositions disclosed herein can also be used in cosmetics, topical skin preparations, quasi-drugs, pharmaceuticals, and the like. The compositions of this technology can be used to manufacture these products.

[0036] The compositions disclosed herein are particularly suitable for use in cosmetics and quasi-drugs. Specifically, the cosmetics and quasi-drugs may take any of the following forms: basic cosmetics such as lotions, creams, toners, serums, packs, and cleansers; makeup cosmetics such as foundations, blushes, and lipsticks; hair care cosmetics such as hair tonics, shampoos, and conditioners; dispersions, ointments, liquids, tablets, aerosols, patches, poultices, liniments, etc.

[0037] In some embodiments, the compositions of the present disclosure are compositions for converting absorbed near-ultraviolet light into visible light. Therefore, when the compositions of the present disclosure are applied to the skin, harmful near-ultraviolet light can be converted into visible light.

[0038] In some embodiments, the compositions of this disclosure are compositions for absorbing near-ultraviolet light and emitting blue light. The wavelength of blue light is typically around 430 nm to 490 nm. Since it has been reported that skin dullness disappears and transparency appears under moderate blue light, the compositions of this disclosure can convert absorbed near-ultraviolet light into useful light.

[0039] The following examples are for illustrative purposes only and are not intended to limit the technical scope of the present invention in any way. Unless otherwise specified, reagents may be commercially available or obtained or prepared by methods commonly used in the art or by procedures in known literature. [Examples]

[0040] Example 1: Preparation of a collagen peptide complex solution 1. Method 1-1. Preparation of collagen peptide complex solution (I) A hydrolyzed collagen peptide solution derived from fish scales with a molecular weight of approximately 3000 (collagen peptide concentration in the solution: 10% by mass) and (II) an aqueous collagen peptide solution were prepared by adding xylose (xylose concentration in the aqueous collagen peptide solution: 1% by mass) to (I). To each collagen solution, alum (potassium aluminum sulfate), zinc chloride, ferric chloride, and copper sulfate were added so that their concentrations in the collagen peptide complex solution were 1% by mass, 1% by mass, 0.5% by mass, and 0.5% by mass, respectively, to prepare a collagen peptide complex solution.

[0041] 1-2. Measurement of fluorescence spectra The fluorescence spectra of each collagen peptide complex solution prepared in 1-1 were measured at 310-370 nm excitation using a fluorescence spectrophotometer (JASCO FP-8600). The obtained data were plotted in three dimensions and compared.

[0042] 2.Results Figures 1(A)-(C) show the three-dimensional fluorescence spectra of aqueous solutions containing collagen peptide alone, aqueous solutions containing collagen peptide, sugar, and alum, and aqueous solutions containing collagen peptide, sugar, and zinc chloride. The fluorescence of collagen peptide alone was not strong (Figure 1(A)), but the addition of xylose slightly increased the visible-range emission, and this trend was further enhanced by the addition of alum (Figure 1(B)). Enhancement of visible-range emission was also observed with the addition of other transition metals, but the enhancement of visible-range fluorescence was particularly pronounced with zinc, showing a fluorescence enhancement effect far exceeding that of alum (Figure 1(C)).

[0043] Figures 2(A)-(C) show the fluorescence spectra of aqueous solutions containing collagen peptide alone, aqueous solutions containing collagen peptide, sugar, and alum, and aqueous solutions containing collagen peptide, sugar, and zinc chloride, at different excitation wavelengths. It was found that fluorescence emission on the longer wavelength side of the visible region boundary (380 nm) was particularly enhanced at the 330-350 nm excitation in the UV-A region.

[0044] From the above findings, it was found that the collagen peptide complex absorbs near-ultraviolet light and emits blue fluorescence. This fluorescence is significantly amplified by the addition of sugar and zinc. This may be related to the increased absorption of near-ultraviolet light in the UVA region due to the additive treatment. The fluorescence spectrum results of this collagen peptide complex suggest that harmful near-ultraviolet light can be converted into useful light.

[0045] Example 2: Manufacturing of a beauty serum Components (1) to (10) from Table 1 were added to the main tank, uniformly stirred, and dissolved. After confirming dissolution, the copolymer of component (11) was added and stirred. The mixture was stirred in a homogenizer at 4000 rpm for 20 minutes to confirm uniform dispersion, then component (12) was added and uniformly dispersed to obtain a jelly-like solution containing collagen peptides. Component (2) is the collagen peptide complex solution of Example 1, containing collagen peptides, sugar, and zinc chloride. This collagen peptide-containing solution emitted blue light, as shown in the container on the right in Figure 3. The container on the left in Figure 3 contains a comparative example solution in which 20.00% by weight of the collagen peptide complex solution and 20.00% by weight of water are present in the composition of Table 1, and no blue light was observed.

[0046] [Table 1]

Claims

1. A composition comprising (A) hydrolyzed collagen with a molecular weight of 500 to 8000, (B) monosaccharides or oligosaccharides with a degree of polymerization of 2 to 4, and (C) transition metal ions.

2. The composition according to claim 1, which is a composition for converting absorbed near-ultraviolet light into visible light.

3. The composition according to claim 1, which is a composition for absorbing near-ultraviolet light and emitting blue light.

4. A composition according to any one of claims 1 to 3, which is a cosmetic composition or a quasi-drug.

5. (B) The composition according to any one of claims 1 to 3, wherein the monosaccharide comprises xylose.

6. (C) The composition according to any one of claims 1 to 3, wherein the transition metal ion comprises a zinc ion.

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