Magnetic nanoparticles sequentially irradiated by laser irradiation for medical, chemical, biological, or cosmetic applications.

Abstract

This invention relates to magnetosomes used in medical, biological, chemical, or cosmetic treatments using sequential laser irradiation. [Solution] Magnetosomes are placed in a part of an individual's body, and in the first step, the magnetosomes are irradiated with a laser beam of first power, and in the second step, the magnetosomes are irradiated with a laser beam of lower power than in the first step, or are not irradiated with a laser. The sequence consisting of the first and second steps is repeated at least once.

Claims

1. In medical, biological, chemical, or cosmetic treatments involving sequential laser irradiation A method of using magnetosomes, wherein the magnetosomes are administered to a part of an individual's body, and In the first step, the magnetosome is irradiated with laser radiation at a first power level. In the second step, the magnetosomes are subjected to lower power rays than in the first step. Either the magnetosomes are irradiated by laser irradiation, or they are not irradiated with laser, The sequence comprising the first and second steps is repeated at least once. How to do it.

2. A method of using a magnetosome according to claim 1, wherein the magnetosome is a living organism, in particular magnetotaxis. A method of synthesis using sex cells.

3. A method of using a magnetosome according to any of the earlier claims, wherein the first... The step further involves heating or dissociating at least one compound from the magnetosome. This method includes both.

4. A method for using magnetosomes according to claim 3, wherein the heating in the first step is as follows: ープ (1) Average heating temperature of 100 degrees Celsius or less, (2) An average heating temperature lower than the highest temperature reached during processing, (3) Maximum heating temperature of 100 degrees Celsius or less, (4) Some heating temperature gradients greater than 2, and (5) Several heating temperatures achieved by continuous irradiation of the magnetosomes with a laser After at least two steps consisting of irradiation with a gradient greater than 10 degrees, the magnetosomes are treated. Several heating temperature gradients are achieved by sequentially irradiating with a laser. A method carried out at a heating temperature characterized by at least one property selected from,

5. A method of using a magnetosome according to any of the earlier claims, wherein the second step The process involves cooling or non-dissociation of at least one compound from the magnetosome, or both. A method of being composed of these.

6. A method for using a magnetosome according to claim 5, wherein the cooling in the second step is The group below (1) Average cooling temperature higher than 0 degrees Celsius, (2) Some cooling temperature gradients greater than 2, A method carried out at a cooling temperature characterized by at least one characteristic selected from,

7. A method of using a magnetosome according to claim 5 or claim 6, wherein the second step The top is under the presence of an object or device to lower the temperature of a body part or magnetosome. It was executed there, i) The second step is present when such an object or device does not exist. It becomes shorter depending on the time, ii) The difference between the highest and lowest temperatures in the second step becomes larger 、 iii) The output power of the laser used to irradiate the magnetosomes during the first step is The level decreases when such an object or device is present compared to when it is not present. iv) The concentration of magnetosomes in body parts is such that such an object or device It is lower when it exists than when it does not exist, and v) The number of sequences performed during at least one session is such as The amount is greater when a body or device is present than when it is absent. How to do that.

8. A method of using a magnetosome according to any of the earlier claims, wherein the first step The first step involves heating, and the second step involves cooling, with the heating temperature of the first step and the The cooling temperature in step 2 is stable or stable in step 1 or step 2. 10 in less than 99.9% of the period 5 , 10 3 , 10, 1 or 10 -1 A method that involves fluctuations less than °C.

9. A method of using a magnetosome according to any of the earlier claims, wherein the first step Or the aforementioned second step or both, (1) 10 -50 and 10 50 It is a period of minutes, and (2) The period between the steps separating the first step and the second step is 、 i) 10 -10 and 10 20 For a few minutes, or ii) Shorter than the duration of the first or second step A method like that.

10. A method of using a magnetosome according to any of the earlier claims, wherein the first... At least one of the sequences consisting of step and the second step is (1) 10 -50 minutes and 10 50 the period of the sequence between minutes, (2) 10 -10 minutes and 10 20 The period between two sequences or between sequences is a period of minutes. (3) A period of at least one sequence that is longer than the period of at least one step, and hand (4) shorter than the duration of at least one sequence or at least one step The period between at least one sequence, A method characterized by at least one property selected from a group consisting of the following.

11. A method of using a magnetosome according to any of the earlier claims, wherein at least A sequence of events constitutes a treatment session, in which the treatment session consists of the following: group, (1) Each treatment session is 10 -50 minutes and 10 5 It has a duration of a minute and consists of two treatment sessions. There is a period between sessions, and it is 10 -10 minutes and 10 20 It is a minute's time. (2) At least one session is longer than the duration of at least one sequence. and (3) There is at least one session interval between two sessions, and it is small At the very least, it has a duration longer than the duration of a single sequence. A method characterized by at least one property selected from among them.

12. A method of using a magnetosome according to any of the earlier claims, wherein the first Step or the second step or both of these steps involve at least one from a magnetosome. The dissociation of one of the compounds reaches a given percentage, or the predetermined temperature GT of the first step is reached. FS , or the predetermined temperature GT of the second step SS Ends when either or both are reached. , here GT SS and GT FS The following groups, (1) GT SS is GT FS It is above, (2) GT SS From GT FS Subtracting that gives 10 -5 and 10 5 Between °C, (3) GT SS or GT FS Or both are -273 and 10 3 Between °C, (4) GT SS It is within the temperature range reached during thermotherapy. (5) GT FS a) i) -40 °C, ii) 0 °C, or iii) higher than 37 °C (6) GT SS and GT FS i) 100 °C, or ii) below the excision temperature or 70 °C, (7) GT SS and GT FS is 10 5 , 10 3 , 10, 5, 2 or 1 °C lower or 37 °C or physiological temperature Higher than degrees A method characterized by at least one property selected from among them.

13. A method of using a magnetosome according to any of the earlier claims, wherein at least Another sequence application is to the following group: i) Dispersion of magnetosomes outside of the body part containing them. decrease, ii) Increase in the percentage of dissociation of at least one compound from the magnetosome, iii) An increase in the numerical value of the temperature gradient or fluctuation, and iv) A decrease in temperature reached during treatment, A method characterized by at least one of the following properties.

14. A method of using a magnetosome according to any of the earlier claims, wherein at least One sequence consists of the following steps: i) The highest temperature or maximum percentage of dissociation in the first step prior to treatment and determination of the lowest temperature or minimum percentage of dissociation in the second step, ii) The highest temperature or the maximum percentage of dissociation in the first step, or both. To reach the target, the laser parameters are set or fixed to a first value, and furthermore, Subsequently, in order to reach the lowest temperature or the minimum percentage of dissociation in the second step, or both: Therefore, set or fix the laser parameters to a second value. iii) Optionally, the first step or the second step or The periods of both are defined as the time it takes to reach their highest or lowest temperature or maximum or minimum dissociation percentage. Measure only as much as needed, and iv) Optionally, the first step or the second step or The first step or the second step or both are repeated while measuring the duration of both. thing, A method carried out according to at least one of the following.

15. A method of sequentially irradiating magnetosomes with a laser, i) A first irradiator that irradiates the magnetosome with laser radiation at a first power Perform the steps, ii) Optionally, the irradiated magnetosaw obtained in the first step Either do not irradiate the magnetosomes, or use the irradiated magnetosomes obtained in the first step as described above. Irradiate with a second laser output that is lower than the first output, iii) Repeat the above step or both steps in at least one sequence. A method consisting of the following.

16. Magnetosomes irradiated according to claim 15, comprising the following groups: (1) The size of the irradiated magnetosomes is greater than that of the unirradiated magnetosomes. ri10 -3 The percentage is smaller than % and 99.99%, where this percentage is S I S NI Ratio to Ta is S NI From S I S NI It is the ratio to S NI and S I These are non-irradiated and irradiated, respectively. The size of the gravitational magnetosome, (2) The number of irradiated compounds wrapped around the irradiated magnetosome n I This is a non-irradiated magnetosaw. The number of compounds wrapped around the tube is n I Smaller, here n NI / n I 1 and 10 10 Between the dots, (3) At least one bond between the irradiated compound and the irradiated magnetosome destroy (4) The binding dissociation energy E between the irradiated compound and the irradiated magnetosome dI teeth , the binding dissociation energy E between the non-irradiated compound and the non-irradiated magnetosome dNI , smaller (5) Thickness of the coating of irradiated magnetosomes (CT) I This is the non-irradiated magnetosome Coating thickness CT NI Thinner, (6) The percentage of the mass of organic material or carbon in the irradiated magnetosome is the same as that of the unirradiated magnetosome. Irradiated magnetosomes with a percentage of organic material or carbon mass less than that of netosomes. The scattered particles of the irradiated compound wrapped around the non-irradiated magnetosomes are unirradiated Smaller than the scattered particles of the mixture, (7) Irradiated compound N from irradiated magnetosomes 2I Irradiated, which hinders the release Number of compounds N 1I This is a non-irradiated compound N from non-irradiated magnetosomes. 2NI Non-irradiated release hinders the release of Number of compounds N 1NI less In addition, non-irradiated magnetosomes are those that are not exposed to laser irradiation or continuous laser irradiation. Magnetosomes exposed to irradiation, Irradiated magnetosomes possessing at least one of the following characteristics.

17. Irradiated magnetosome according to claim 16, wherein the magnetosome has a biological characteristic Magnetosomes are synthesized by magnetotactic bacteria.

18. Compounds, particularly those for cosmetic, medical, or diagnostic purposes, which are irradiated according to claim 16 or 17. Magnetosomes and the following groups, (1) The color of the body parts, (2) Contrast of the body parts (3) The contrast-enhancing ability of the aforementioned body parts, and (4) Ability to treat or repair the aforementioned body parts at least one for changing the properties of at least one of the body parts selected from Substances that color or enhance contrast, or diagnostic substances, or therapeutic substances. It is composed of quality.

Images