Topical skin preparations

Abstract

To provide an active ingredient for a skin external preparation which is derived from a natural product, has excellent biological safety, prevents and mitigates skin inflammation or aging caused by internal factors such as aging or external factors such as UV light and environmental pollutants, keeps skin young and healthy, and makes skin smooth.SOLUTION: The skin external preparation comprises green rooibos extract as an active ingredient.SELECTED DRAWING: None

Description

Technical Field

[0001] The present invention relates to a topical skin preparation containing a plant-derived component as an active ingredient.

Background Art

[0002] In recent years, from the viewpoint of biological safety, development of various plant-derived components as ingredients of topical skin preparations has been promoted. However, plant-derived components have had problems in terms of effectiveness and stability. In particular, in the case of plant-derived components, there have been problems such as the possibility of not exhibiting sufficient effectiveness.

Disclosure of the Invention

Problems to be Solved by the Invention

[0003] In view of the problems of such prior art, the present inventors newly found that an extract of green rooibos is useful as an ingredient of excellent cosmetics.

[0004] Conventionally, although topical skin preparations containing an extract of rooibos as an active ingredient are known from, for example, Patent Documents 1 to 3, topical skin preparations containing an extract of green rooibos as an active ingredient have not been known. Further, although a skin tissue protectant containing asparagine contained in green rooibos as an active ingredient is also known from Patent Document 4, asparagine is extremely easily decomposed and has a problem in stability as an active ingredient of a topical skin preparation.

[0005]

Patent Document 1

Patent Document 2

Patent Document 3

Patent Document 4

Means for Solving the Problems

[0006] The present invention relates to a topical skin preparation containing an extract of green rooibos. [Effects of the Invention]

[0007] This invention provides a topical skin preparation with excellent biocompatibility and efficacy by using an extract of green rooibos as the active ingredient. [Modes for carrying out the invention]

[0008] Preferred embodiments of the present invention will be described in detail below. First, the extracting material, rooibos (Aspalathus linearis), is a plant belonging to the genus Aspalathus in the legume family. In this invention, unfermented, green rooibos (green rooibos) is used. The whole plant or leaves are preferred as the part to be used.

[0009] The green rooibos used as the extraction material may be fresh, or it may be pre-dried or semi-dried. In terms of form, it can be used as is after harvesting, or as dried and ground rooibos.

[0010] The extraction is prepared by washing the material as needed to remove impurities, then either leaving it as is or drying it, and then finely chopping or grinding it as needed, before contacting it with the extraction solvent for extraction. Extraction can be carried out by contacting the material with the extraction solvent according to conventional methods such as immersion, but supercritical fluid extraction can also be used in addition to immersion.

[0011] Examples of extraction solvents include water; lower alcohols such as methanol, ethanol, and propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol, and glycerin; esters such as ethyl acetate, butyl acetate, and methyl propionate; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; and hydrocarbon solvents such as n-hexane, toluene, and chloroform. These can be used individually or in combination of two or more.

[0012] Among these extraction solvents, hydrophilic solvents such as water, lower alcohols, or polyhydric alcohols are preferred in the present invention, from the viewpoint of skin irritation and effectiveness, as well as their broad applicability to cosmetics. Preferred examples of using these hydrophilic solvents include, for example, the use of water, lower alcohols (especially ethanol), or polyhydric alcohols (especially 1,3-butylene glycol) alone, or a mixed solvent of water and lower alcohols (especially ethanol), or a mixed solvent of water and polyhydric alcohols (especially 1,3-butylene glycol, glycerin), but among these, water alone or a mixed solvent of water and 1,3-butylene glycol are particularly preferred.

[0013] When using a mixed solvent, the mixing ratio is preferably in the range of 1:10 to 20:1 by volume (hereinafter the same) for a mixed solvent of water and 1,3-butylene glycol, 1:10 to 25:1 for a mixed solvent of water and ethanol, and 1:10 to 20:1 for a mixed solvent of water and glycerin.

[0014] While there are no particular limitations on the pH of the extract during preparation, it is generally preferable to have a pH in the range of 3 to 9. In this sense, if necessary, the extraction solvent may be adjusted to the desired pH by adding an alkaline adjusting agent such as sodium hydroxide, sodium carbonate, or potassium hydroxide, or an acidic adjusting agent such as citric acid, hydrochloric acid, phosphoric acid, or sulfuric acid.

[0015] Extraction conditions such as extraction temperature and extraction time vary depending on the type of solvent and pH used. For example, when using water, 1,3-butylene glycol, or a mixture of water and 1,3-butylene glycol as the solvent, the extraction temperature is preferably in the range of 0°C to 90°C. The extraction time is preferably in the range of 1 to 168 hours (1 hour to 1 week).

[0016] The extract prepared as described above may generally be used as a topical skin preparation after adjusting the pH to 3-8, or it may be concentrated to a desired concentration by vacuum concentration or other means. The extract may also be dried by conventional methods such as spray drying.

[0017] Examples of topical skin preparations (topical medicinal components, topical quasi-drugs, and cosmetics) containing the extract of the present invention include, but are not limited to, emulsions, creams, lotions, essences, gels, packs, sheet masks, lipsticks, foundations, liquid foundations, makeup pressed powders, blushes, face powders, facial cleansers, body shampoos, slimming agents, hair shampoos, and soaps. They also include, but are not limited to, hair growth and hair nourishing agents, as well as bath additives.

[0018] In topical skin preparations (topical medicinal components, topical quasi-drugs, and cosmetics), the amount of the active ingredient according to the present invention is generally in the range of 0.0002 to 1.0% by weight (solids by weight, the same applies hereinafter) as solid content in the case of basic cosmetics, generally in the range of 0.0002 to 1.0% by weight, preferably in the range of 0.002 to 0.2% by weight in the case of makeup cosmetics, and generally in the range of 0.002 to 10.0% by weight in the case of cleansing cosmetics. In the case of hair cosmetics, the solid content of the extract is generally 0.00001 to 5.0% by weight, preferably 0.0001 to 3.0% by weight.

[0019] Here, oily components include, for example, plant-derived oils and fats such as olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, coconut oil, palm oil, cocoa oil, meadowfoam oil, shea butter, tea tree oil, avocado oil, macadamia nut oil, bergamot oil, lavender oil, rose oil, chamomile oil, etc.; vitamin A oil; animal-derived oils and fats such as mink oil, turtle oil, etc.; waxes such as beeswax, carnauba wax, rice wax, lanolin, etc.; liquid paraffin, petrolatum, paraffin wax. Examples include hydrocarbons such as custard and squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, and cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, pantothenyl alcohol, and stearyl alcohol; synthetic esters and synthetic triglycerides such as isopropyl myristate, isopropyl palmitate, butyl oleate, 2-ethylhexylglyceride, and higher fatty acid octyldodecyl (octyldodecyl stearate, etc.).

[0020] As surfactants, for example, nonionic surfactants such as polyoxyethylene alkyl ethers, polyoxyethylene fatty acid esters, polyoxyethylene sorbitan fatty acid esters, glycerin fatty acid esters, polyglycerin fatty acid esters, polyoxyethylene glycerin fatty acid esters, polyoxyethylene hydrogenated castor oil, polyoxyethylene sorbitol fatty acid esters; anionic surfactants such as fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkyl phenyl ether phosphates; cationic surfactants such as quaternary ammonium salts, primary to tertiary fatty amine salts, trialkylbenzylammonium salts, alkylpyridinium salts, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salts, N, N-dialkylmorpholinium salts, polyethylene polyamine fatty acid amide salts; amphoteric surfactants such as N, N-dimethyl-N-alkyl-N-carboxymethylammonio betaine, N, N, N-trialkyl-N-alkylene ammoniocarboxy betaine, N-acylamidopropyl-N′, N′-dimethyl-N′-β-hydroxypropylammonio sulfobetaine, etc. can be used.

[0021] As emulsifiers and / or emulsification aids, it is also possible to incorporate stevia derivatives such as enzymatically treated stevia, saponin or its derivatives, casein or its salts (such as sodium), complexes of sugars and proteins, sucrose or its esters, lactose, water-soluble polysaccharides derived from soybeans, complexes of soy-derived proteins and polysaccharides, lanolin or its derivatives, cholesterol, stevia derivatives (such as enzymatically treated stevia), silicates (such as aluminum, magnesium), carbonates (such as calcium, sodium), saponin and its derivatives, lecithin and its derivatives (such as hydrogenated lecithin), lactic acid bacteria-fermented rice, lactic acid bacteria-fermented germinated rice, lactic acid bacteria-fermented grains (such as wheat, beans, miscellaneous grains), etc.

[0022] Examples of moisturizers include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, etc. Further examples include saccharides such as trehalose and raffinose, mucopolysaccharides (e.g., hyaluronic acid and its derivatives, hyaluronic acid fermentation broth, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, collagen peptides, NMF-related substances, lactic acid, urea, octyldodecyl higher fatty acid, seaweed extract, estradiol, various amino acids and their derivatives.

[0023] Examples of thickeners include components derived from brown algae, green algae or red algae such as alginic acid, agar, carrageenan, fucoidan, etc.; polysaccharides such as pectin and aloe polysaccharide; gums such as tragacanth gum, locust bean gum, xanthan gum, guar gum, etc.; cellulose derivatives such as carboxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, etc.; synthetic polymers such as carboxyvinyl polymer, alkyl-modified carboxyvinyl polymer, polyvinyl alcohol, polyvinyl pyrrolidone, acrylic acid-methacrylic acid copolymer, etc.; hyaluronic acid and its derivatives; polyglutamic acid and its derivatives, polyacrylic acid, etc.

[0024] Examples of anti-inflammatory agents include allantoin, dipotassium glycyrrhizinate, monoammonium glycyrrhizinate, β-glycyrrhetinic acid, stearyl glycyrrhetinate, ε-aminocaproic acid, d-camphor, dl-camphor, zinc oxide, panthenol, pyridoxine hydrochloride, and riboflavin or its derivatives, etc.

[0025] Examples of preservatives and disinfectants include urea; benzoic acid or its salts, parahydroxybenzoic acid esters such as methyl parahydroxybenzoate, ethyl parahydroxybenzoate, propyl parahydroxybenzoate, and butyl parahydroxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzalkonium chloride, salicylic acid, sodium salicylate, zinc pyrithione, benzalkonium chloride, ethanol, undecylenic acid, phenols, and aluminum bromide. These include chloroisoquinolinium, resorcinol, jamal (imidazodinylurea), isopropylmethylphenol, triclosan, trichlorocarbanide, trichlorohydroxydiphenol ether, hinokitiol, 1,2-pentanediol, propanediol, concentrated benzalkonium chloride solution 50, essential oils such as peppermint oil and eucalyptus oil, tree bark distillates, radish ferment filtrate, plant-derived ethanol such as sugarcane and corn, or 1,3-butylene glycol.

[0026] Examples of cell activators include pantothenyl alcohol, menthol, dl-menthol, and γ-oryzanol.

[0027] Examples of anti-acne agents include sulfur, salicylic acid or its salts, photosensitizer 201, and pyridoxine dicaprylate.

[0028] Examples of powder components include sericite, titanium dioxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, anhydrous silicic acid, mica, nylon powder, polyethylene powder, silk powder, cellulose-based powders, grain powders (rice, wheat, corn, millet, etc.), and legume powders (soybeans, adzuki beans, etc.).

[0029] Examples of UV absorbers include ethyl para-aminobenzoate, ethylhexyl para-dimethylaminobenzoate, amyl salicylate and its derivatives, 2-ethylhexyl para-methoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonate, 4-tert-butyl-4-methoxybenzoylmethane, 2-(2-hydroxy-5-methylphenyl)benzotriazole, urocanic acid, ethyl urocanate, and aloe extract.

[0030] Examples of antioxidants include carotenoids such as butylhydroxyanisole, butylhydroxytoluene, propyl gallate, and astaxanthin, vitamin E and its derivatives (e.g., tocopherol acetate, tocopherol nicotinate), and vitamin A or its derivatives (e.g., retinol palmitate).

[0031] Furthermore, examples of whitening agents include one or more selected from ellagic acid and its derivatives, resorcinol derivatives, potassium 4-methoxysalicylate, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acids, nicotinic acid derivatives, and AMP (adenosine monophosphate, adenosine monophosphate).

[0032] Examples of resorcinol derivatives include 4-n-butylresorcinol and 4-isoamylresorcinol; examples of 2,5-dihydroxybenzoic acid derivatives include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, and 2-hydroxy-5-propionyloxybenzoic acid; and examples of α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid. One or more substances selected from kojic acid and its derivatives, ascorbic acid and its derivatives, hydroquinone or its derivatives, ellagic acid and its derivatives, nicotinic acid and its derivatives, resorcinol derivatives, tranexamic acid and its derivatives, potassium 4-methoxysalicylate, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acids, AMP (adenosine monophosphate, adenosine monophosphate), t-cycloamino acid derivatives, mulberry bark extract, chamomile extract, hydrolyzed rice bran extract, saxifrage extract, and white poppy extract or their hydrolyzed products.

[0033] Examples of kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, and kojic acid dibutyrate, kojic acid ethers, and kojic acid sugar derivatives such as kojic acid glucoside. Examples of ascorbic acid derivatives include ascorbic acid ester salts such as L-ascorbic acid-2-phosphate sodium, L-ascorbic acid-2-phosphate magnesium, L-ascorbic acid-2-sulfate sodium, and L-ascorbic acid-2-sulfate magnesium. Ascorbic acid sugar derivatives such as ascorbic acid-2-glucoside, L-ascorbic acid-5-glucoside, ascorbyl tocopheryl maleate, ascorbyl tocopheryl potassium phosphate, myristyl 3-glyceryl ascorbate, caprylyl 2-glyceryl ascorbate, 6-position acylated products of these ascorbic acid sugar derivatives (acyl groups include hexanoyl, octanoyl, and decanoyl groups), L-ascorbic acid tetra fatty acid esters such as L-ascorbic acid tetraisopalmitate and L-ascorbic acid tetralaurate, 3 As for hydroquinone derivatives, examples include -O-ethyl ascorbic acid, L-ascorbic acid-2-phosphate-6-O-palmitate sodium, glyceryl ascorbic acid or its acylated derivatives, ascorbic acid glycerin derivatives such as bisglyceryl ascorbic acid, L-ascorbic acid aminopropyl phosphate, hyaluronic acid derivatives of L-ascorbic acid, 3-OD lactose-L-ascorbic acid, isostearyl ascorbyl phosphate, etc. Hydroquinone derivatives include arbutin (hydroquinone-β-D-glucopyranoside), α-arbutin (hydroquinone-α-D-glucopyranoside), Examples of tranexamic acid derivatives include lucopyranoside, tranexamic acid esters (e.g., lauryl tranexamic acid ester, hexadecyl tranexamic acid ester, cetyl tranexamic acid ester or their salts), amide forms of tranexamic acid (e.g., methyl tranexamic acid amide), resorcinol derivatives include 4-n-butylresorcinol, 4-isoamylresorcinol, and 2,5-dihydroxybenzoic acid derivatives include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid,Examples of nicotinic acid derivatives include 2-hydroxy-5-propionyloxybenzoic acid, nicotinamide (niacinamide) and benzyl nicotinate, and alpha-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, and alpha-hydroxyoctanoic acid.

[0034] Furthermore, the following animal, plant, or microorganism-derived ingredients can also be used in combination: for example, collagen or its hydrolysates, yeast extracts or hydrolysates, lactic acid bacteria cultures, grasses, cruciferous plants, camellias, roses, peonyes, citrus plants, amaranthaceae plants, eelgrasses, legumes, daisies, legumes, mallows, gentians, mints, lotus, cucurbits, Araliaceae plants, nightshades, bignoniaceae plants, Actinidiaceae plants, mulberries, irises, bellflowers, olives, Actinidiaceae plants, mulberries, rhamnoides, orchids, and sumac. Examples include extracts or hydrolyzed or fermented products thereof from one or more plants selected from the families of plants, Garcinia, Valenciaceae, Rutaceae, Myrtaceae, Liliaceae, Crassulaceae, Cupressaceae, Convolvulaceae, and Asparagaceae; extracts or hydrolyzed or fermented products thereof from one or more seaweeds selected from the families of Laminariaceae, Mylinaceae, and Ulvaceae; jellyfish (autolyzed products of moon jellyfish, Echizen jellyfish, etc.); hydrolyzed or fermented products of hyaluronic acid; and extracts or hydrolyzed or fermented products thereof from royal jelly.

[0035] As plant-derived components from the grass family, rice leaf hydrolysate, rice extract hydrolysate, rice bran extract hydrolysate, germinated brown rice hydrolysate, rice ferment filtrate, sake lees extract derived from sake, bamboo shoot peel extract of Madake or Moso bamboo, and adlay seed ferment. As plant-derived components from the Brassicaceae family, extracts or hydrolysates or fermentations thereof of the seeds of Hakugai, Ougai, or Kokugai are particularly preferred. As plant-derived components from the Camellia family, green tea (Yabukita, Samidori, Asahi, Gokou, Ujimidori, Kyomidori, Ujihikari, Samidori, Benifuki, etc.) and black tea (Darjeeling, Assam, Ceylon, Earl Grey, Honey Black Tea, etc.) are particularly preferred. As plant-derived components from the Rosaceae family, extracts of Damask rose flowers, peach flowers, leaves, or immature fruits, apricot fruit or seed extracts, strawberry flower extracts, and cherry blossom or leaf extracts are preferred. Furthermore, as ingredients derived from Paeoniaceae plants, extracts of peony root or flower, and peony flower or root are preferred. As ingredients derived from Amaranthaceae plants, glasswort extract is particularly preferred. As ingredients derived from Zosteraceae plants, eelgrass or dwarf eelgrass extract is particularly preferred. As ingredients derived from Fabaceae plants, extracts of white soybeans or black soybeans or their hydrolysates or fermented soy milk liquid, adzuki bean extract, red clover extract, and kudzu root extract are particularly preferred. As ingredients derived from Asteraceae plants, burdock root extract, sunflower sprout extract, starflower extract, arnica extract, or chamomile flower extract are particularly preferred. As ingredients derived from Malvaceae plants, fermented products of hibiscus, rose of Sharon, or hibiscus are preferred. As ingredients derived from Gentianaceae plants, gentian extract is preferred. As ingredients derived from Lamiaceae plants, green perilla extract and purple beautyberry fruit extract are preferred. As for ingredients derived from Nelumbonaceae plants, lotus flower or lotus seed extract or lotus seed ferment is particularly preferred. As for ingredients derived from Cucurbitaceae plants, loofah extract is particularly preferred. As for ingredients derived from Araliaceae plants, ginseng extract or ferment is preferred. As for ingredients derived from Solanaceae plants, eggplant (long eggplant, water eggplant, American eggplant, Kamo eggplant, etc.) extract is an example. As for ingredients derived from Bignoniaceae plants, Pau d'arco bark extract is preferred. As for ingredients derived from Actinidiaceae plants, immature kiwi extract is preferred.Preferred ingredients from the Moraceae family include mulberry bark extract, mulberry fruit extract, and fig fruit or bark extract. Preferred ingredients from the Rhamnaceae family include jujube fruit extract. Preferred ingredients from the Iridaceae family include saffron. Preferred ingredients from the Campanulaceae family include root extract or hydrolysate of Codonopsis lanceolata. Preferred ingredients from the Anacardiaceae family include mango fruit extract. Preferred ingredients from the Garciniaaceae family include mangosteen fruit extract. Preferred ingredients from the Valenciaceae family include cherimoya fruit extract. Preferred ingredients from the Rutaceae family include Satsuma mandarin, bergamot fruit extract, grapefruit or pomelo fruit (including immature fruit), extracts containing flavonoids and their glycosides found in plants such as grapefruit or hassaku, or sansho seed extract. As for ingredients derived from plants of the Liliaceae family, extracts of Hemerocallis middendorffii, Hemerocallis fulva, Casablanca lily, Madonna lily, or Lilium japonicum are preferred. As for ingredients derived from plants of the Crassulaceae family, extracts or fermented products of Rhodiola rosea are particularly preferred. As for ingredients derived from plants of the Oleaceae family, extracts of jasmine flowers are particularly preferred. As for ingredients derived from plants of the Cupressaceae family, extracts of Juniperus chinensis fruit are particularly preferred. As for ingredients derived from plants of the Myrtaceae family, extracts of guava leaves are particularly preferred. As for ingredients derived from plants of the Orchidaceae family, extracts of Bletilla striata root are particularly preferred. As for ingredients derived from plants of the Convolvulaceae family, extracts or fermented products thereof of sweet potato, or extracts or fermented products thereof of sweet potato shochu lees are preferred. In addition, as for plants of the Asparagaceae family, asparagus (green asparagus and white asparagus) is preferred. As for the components derived from Laminariaceae seaweed, kelp extract is particularly preferred, as for the components derived from Milinaceae seaweed, sorghum extract is particularly preferred, and as for the components derived from Ulvaceae seaweed, Ulva extract is particularly preferred. As for the components derived from Funoriaceae seaweed, Funori extract is particularly preferred.

[0036] Next, the present invention will be described in more detail with reference to manufacturing examples, formulation examples, and test examples, but the present invention is not limited thereto. In the following, all parts refer to parts by weight, and all percentages refer to weight percent.

[0037] Manufacturing Example 1. Preparation of Extract (1) 100g of dried whole plant of unfermented rooibos (green rooibos), a plant belonging to the genus Aspalathus in the legume family, was mixed with 1000g of purified water and 1,3-butylene glycol (5:5) and extracted at 80°C for 2 hours. Insoluble matter was removed by filtration to obtain 1025g of dark brown green rooibos extract (solid content concentration 1.22%).

[0038] Manufacturing Example 2. Preparation of Extracts (2) In the extraction method of Production Example 1, instead of the mixed solvent of purified water and 1,3-butylene glycol (7:3), 1000g of purified water was added and extracted at 80°C for 2 hours. Insoluble matter was then removed by filtration to obtain 1030g of dark brown green rooibos extract (solid content concentration 1.30%).

[0039] Test Example 1. Evaluation Test for Various Gene Expressions Human dermal fibroblasts NB1RGB were cultured in Eagle Minimum Essential Medium containing 0.5% NCS (6 × 10⁶ cells). 4The culture solution was prepared at a concentration of 1 / mL, and 1 mL was seeded into each 24-well plate. The cultures were incubated at 37°C under 5% CO2 and saturated steam. After 24 hours of incubation, the culture was further incubated with a culture solution containing the extract from Production Example 1 (the extract from Production Example 1 was added to the total culture solution to achieve final concentrations of 0.1% and 0.2%). As a control, a test group (control group) was set up in which a culture solution containing only a 30% 1,3-butylene glycol aqueous solution (adjusted to a final concentration of 0.2% of the total culture solution) was added instead of the extract from Production Example 1. After 24 hours of incubation, the cultures were incubated for another 24 hours under irradiation with 5 J of ultraviolet A light using an ultraviolet lamp (AS ONE, model SLUV-6). Cells from each test group were collected using Trizol reagent (0.5 mL, Invitrogen). 100 μL of chloroform (Wako Pure Chemical Industries, Ltd.) was added to the collected cells and mixed. The mixture was then centrifuged at 15,000 rpm at 4°C for 15 minutes using a TOMY MX-160 centrifuge, and 200 μL of the aqueous layer was collected. 250 μL of isopropanol (Wako Pure Chemical Industries, Ltd.) was added to the collected aqueous layer and mixed. The mixture was then centrifuged at 15,000 rpm at 4°C for 15 minutes to obtain a total RNA precipitate. 1 mL of 75% ethanol was added to the total RNA and mixed to wash it. The precipitate was then collected by centrifugation at 15,000 rpm at 4°C for 15 minutes. The collected total RNA was then processed using the specified kit (PrimeScript RT reagent Kit with gDNA Eraser (Perfect Real cDNA was synthesized by reverse transcription using Time (manufactured by Takara Bio Inc.). The synthesized cDNA was used as a sample to detect the expression of various genes and the expression of the internal standard substance β-actin gene using Thermal Cycler Dice® Real Time System Single (manufactured by Takara Bio Inc.) and SYBR® Premix Ex Taq™ II (Perfect Real Time) (manufactured by Takara Bio Inc.).Here, β-actin is one of the housekeeping genes (genes that are consistently expressed in a certain amount in many tissues and cells, and are always expressed and essential for cell maintenance and proliferation), and because its expression level is always constant, it is used as an internal standard in PCR experiments. The test results compare the expression levels of each gene in each test group when the expression level of the β-actin gene is kept constant. In this test system, the relative value of the expression level of each gene in the other test groups was calculated when the expression level of each gene in the control group was set to 100.

[0040] The results of Test Example 1 are shown below. [Table 1] JPEG0007855211000001.jpg55154

[0041] As shown in Table 1 above, the extract according to the present invention was confirmed to have a remarkably superior inhibitory effect on the expression of cytokine (IL-8, IL-1β) genes, which are inflammatory factors in the skin. This suggests that the extract according to the present invention has an effect of suppressing skin inflammation.

[0042] Test Example 2. Evaluation test of the effect of inhibiting neutrophil-like cell migration activity. Human dermal fibroblasts NB1RGB were placed in 9 x 10⁶ wells of a 24-well microplate in Eagle Minimum Essential Medium containing 10% NCS. 4 Cells were seeded per well and pre-cultured for 1 day under conditions of 37°C and 5.0% CO2. Then, Production Example 1 was added to the culture medium at concentrations of 0.1% and 0.2% (as solution) and cultured further. The following day, 5 J of UVA was irradiated using a UV lamp (AS ONE, model SLUV-6), and the culture supernatant 24 hours after the end of irradiation was used as the cytokine induction solution. Simultaneously, a control group that was not irradiated with UV light was established, and the collected culture supernatant was used as the cytokine non-inducing solution. 500 μL of the collected culture solution was added to the receptor side of a Transwell (Corning, model 3415). Human leukemia cell line HL60 cells, differentiated into neutrophil-like cells by culturing in RPMI medium containing 1.25% DMSO and 10% FBS for 5 days, were divided into 1 × 10⁶ cells. 6The cells were adjusted to a concentration of cells / mL and seeded in 300 μL on the cell culture insert side of a Transwell. Two hours after seeding differentiated HL60 cells, cells that had migrated to the receptor side were obtained. In addition, 200 μL of 0.5 mM EDTA / PBS(-) solution was added to a separate well from the seeded cells, and the cell culture insert, after migration had finished, was moved and allowed to stand for 30 minutes to obtain the cells remaining at the bottom of the cell culture insert. After combining the migrated cells and allowing them to take up 2',7'-dichlorodihydrofluorescein diacetate, the fluorescence intensity (excitation wavelength 485 nm, absorption wavelength 538 nm) was measured using a fluorescence plate reader (Fluoroscan Ascent, Thermo Labsystems). The obtained fluorescence intensity is shown as a relative value with the cytokine-uninduced control set to 100.

[0043] The results of Test Example 2 are shown in Table 2. [Table 2] JPEG0007855211000002.jpg56150

[0044] As shown in Table 2, the present invention has been confirmed to have a remarkably superior inhibitory effect on neutrophil-like cell migration activity. Inhibition of neutrophil-like cell migration activity means suppressing the infiltration of neutrophils into the inflammatory site by cytokines secreted from dermal-derived fibroblasts. Therefore, the effects of the present invention suggest that it has an effect of improving wrinkles and sagging, as it suppresses the infiltration of neutrophils into the inflammatory site and inhibits the secretion of collagen and elastin-degrading enzymes by neutrophils at the inflammatory site.

[0045] Test Example 3. IL-8 Secretion Suppression Test Human dermal fibroblasts NB1RGB were placed in 9 x 10⁶ wells of a 24-well microplate in Eagle Minimum Essential Medium containing 10% NCS. 4Seeds were sown per hole and pre-cultured for 1 day under conditions of 37°C and 5.0% CO2. Then, Production Example 1 was added to the culture medium at concentrations of 0.1% and 0.2% (as solution) and cultured further. The following day, 5 J of ultraviolet A light was irradiated using an ultraviolet lamp (AS ONE, model SLUV-6), and the culture supernatant 24 hours after the end of irradiation was used as the IL-8 induction solution. Simultaneously, a control group without ultraviolet irradiation was established, and the collected culture supernatant was used as the IL-8 non-inducible solution. The amount of IL-8 in the collected culture solution was measured using an IL-8 measurement kit (Boster Biological Technology). The obtained IL-8 amounts are shown as relative values ​​with the non-inducible control set to 100.

[0046] The results of Test Example 3 are shown in Table 3. [Table 3]

[0047] As shown in Table 3, the present invention has been confirmed to have a remarkably superior effect in suppressing IL-8 secretion. This suggests that the present invention has an effect in suppressing skin inflammation.

[0048] Furthermore, the extract of the present invention may be incorporated into topical skin preparations in combination with other active ingredients. For example, the extract of the present invention, which inhibits the degradation of extracellular matrix components of the skin, may be used in combination with an ingredient that promotes collagen synthesis in the dermis (e.g., niacinamide).

[0049] Test Example 4. Collagen Synthesis Promoting Effect Human dermal fibroblast cells NB1RGB were placed in 1 × 10⁶ wells of a 96-well microplate containing 0.5% NCS-containing Eagle Minimum Essential Medium. 4Cells were seeded per well and pre-cultured for 1 day at 37°C and 5.0% CO2. Then, a pre-prepared sample solution (niacinamide) was added to the culture medium in which the cells were pre-cultured to a final concentration of 1 mM to set up the test group. After adding the sample solution, each medium was cultured for a further 5 days under the same conditions as the pre-culture. Next, the medium was removed, the cells were fixed with cold methanol and cold ethanol, and then stained with a saturated picric acid aqueous solution containing 0.1% Sirius Red. After washing with purified water, extraction was performed with a 0.1% NaOH:methanol = 1:1 solution, and the amount of collagen was measured at a wavelength of 540 nm using a microplate reader (Model 680, Bio-Rad). The same procedure was performed for the control group (without sample addition, where medium was added instead of the sample solution), and the relative amount of collagen with each sample added to the amount of collagen obtained here was calculated and expressed as the collagen synthesis rate (%). Furthermore, to confirm that the test system was functioning correctly, the same test was performed when 1 mM magnesium ascorbic acid phosphate (APM) was added as a positive control instead of the sample solution.

[0050] The results of Test Example 4 are shown in Table 4. [Table 4] JPEG0007855211000004.jpg37133

[0051] Combining niacinamide, which has a dermal collagen synthesis promoting effect as shown in Table 4, with the extract according to the present invention suggests that the balance between the generation and degradation of extracellular matrix components can be adjusted, resulting in a more significant synergistic effect on wrinkle improvement.

[0052] Test Example 5. Wrinkle Improvement Test Six subjects (five men and one woman, aged 30-50) had their faces washed and were allowed to acclimate in a room with constant temperature and humidity (20°C ± 2°C, 50% ± 5% humidity) for 15 minutes. Replicas of wrinkles at the corners of the eyes were then taken and designated as the initial replicas. Subsequently, the cream of Formulation Example 15 (Sample 1 of the present invention) and the cream of Formulation Example 15 without the extract of Manufacturing Example 1 (Comparative Sample 1) were applied to the outer corners of the eyes on the designated side twice a day, morning and evening. After eight weeks, the subjects' faces were washed and replicas of wrinkles at the corners of the eyes were taken. The initial replicas and the replicas after eight weeks for each subject were analyzed using the ASA-03RXD reflection replica analysis system (manufactured by Nippon Ash Co., Ltd.). Specifically, when parallel light at a 30-degree angle was shone onto the replicas, the shadows of the replicas were captured as images into a computer, and the volume of the wrinkles was calculated as the average value for the six subjects based on the length of the shadows.

[0053] The results of Test Example 5 are shown in Table 5. [Table 5] JPEG0007855211000005.jpg57131

[0054] As shown in Table 5, Sample 1 of the present invention showed a significant reduction in the volume percentage of wrinkles compared to Comparative Sample 1. This confirms that Sample 1 of the present invention, containing niacinamide and the extract from Production Example 1, exhibits a remarkably superior synergistic effect in wrinkle improvement.

[0055] Prescription example 1. Lotion [Ingredients] Part Eucalyptus oil 0.2 Polyoxyethylene (5.5) cetyl alcohol 5.0 Extract from Production Example 1 2.0 Tocopherol acetate 0.02 Dipotassium glycyrrhizinate 0.5 Monoammonium glycyrrhizinate 0.5 Stearyl glycyrrhetinate 0.05 Isopropylmethylphenol 0.1 Align In 0.1 D-Pantothenyl alcohol 0.1 Salicylic acid 0.5 Urea 5.0 l-Menthol 0.9 dl-menthol 0.2 1,3-Butylene glycol 5.0 Sodium citrate 0.2 Methylparaben 0.1 Hinokitiol 0.003 Photosensor No. 201 0.002 Purified water, in an amount that makes the total volume 100 parts.

[0056] Prescription example 2. Lotion [Ingredients] Part Glyceryl caprylate 3.0 Polyglyceryl-10 laurate 3.0 Cetanol 2.0 Behenyl alcohol 2.0 Methylparaben 0.1 Extract from Production Example 2 2.0 Ascorbic acid 3.0 Glycyrrhizic acid 0.5 β-Glycyrrhetinic acid 0.05 Tocopherol nicotinate 0.1 Resorcinol 0.1 Zinc oxide 2.0 dl-camphor 0.5 Glycerin 2.0 1,3-Butylene glycol 5.0 Potassium hydroxide 0.5 Purified water, in an amount that makes the total volume 100 parts.

[0057] Prescription example 3. Lotion [Ingredients] Part Jojoba oil 1.0 Polyoxyethylene (5.5) cetyl alcohol 5.0 Methylparaben 0.1 Extract from Production Example 1 2.0 Ascorbic acid glucoside 2.0 Tranexamic acid 2.0 ε-aminocaproic acid 0.1 Sulfur 0.2 Estradiol 0.1 Glycerin 5.0 1,3-Butylene glycol 5.0 Sodium citrate 0.2 Sodium metabisulfite 0.2 d-camphor 0.1 Purified water, in an amount that makes the total volume 100 parts.

[0058] Prescription example 4. Emulsion [Ingredients] Part Squalane 5.0 Cyclopentasiloxane 1.0 Hexaran 3.0 Hexyldecyl isostearate 1.0 Caprylic / Capric Triglyceride 1.0 Polyglyceryl-10 laurate 5.0 Polyglyceryl-10 isostearate 5.0 Ascorbyl dipalmitate 15.0 Hydrogenated soy lecithin 1.5 Extract from Production Example 1: 1.0 Magnesium ascorbic acid phosphate salt 3.0 Arbutin 3.0 Potassium hydroxide 0.5 Glycerin 3.0 1,3-Butylene glycol 2.0 Carboxymethylcellulose 0.3 Xanthan gum 0.2 Tremella fuciformis polysaccharide 0.2 Sodium hyaluronate 0.01 Tocopherol acetate 0.3 Tocopherol nicotinate 0.1 Glycyrrhizic acid 0.1 Dipotassium glycyrrhizinate 0.1 Isopropylmethylphenol 0.1 Water-soluble collagen 1.0 Hydrolyzed collagen 1.0 Sodium hyaluronate 1.0 Purified water, in an amount that makes the total volume 100 parts.

[0059] Prescription example 5. Emulsion An emulsion was obtained in the same manner as in Formula Example 4, except that 2.0 parts of L-ascorbic acid-2-glucoside were used instead of 2.0 parts of magnesium ascorbic acid phosphate in Formula Example 4.

[0060] Prescription example 6. Emulsion An emulsion was obtained in the same manner as in Formula Example 4, except that 2.0 parts of tranexamic acid were used instead of 2.0 parts of magnesium ascorbic acid phosphate and 0.5 parts of potassium hydroxide.

[0061] Prescription example 7. Emulsion An emulsion was obtained in the same manner as in Formula Example 4, except that 3.0 parts of nicotinamide were used instead of 2.0 parts of magnesium ascorbic acid phosphate in Formula Example 4.

[0062] Prescription example 8. Cream [Ingredients] Part Olive oil 5.0 Jojoba oil 5.0 Squalane 5.0 Hexyldecyl isostearate 5.0 Lauroyl glutamate di(octyldodecyl / phytosteryl / behenyl) 5.0 Glyceryl caprylate 1.0 Glyceryl stearate 1.0 Isostearyl glyceryl 3.0 γ-oryzanol 0.1 Behenyl alcohol 2.0 Palmitic acid 2.5 D-Panthenyl alcohol 3.0 Allantoin 0.1 Riboflavin 0.01 Resorcinol 0.1 Benzalkonium chloride 0.05 Urea 3.0 β-Glycyrrhetinic acid 0.1 Stearyl glycyrrhetinate 0.1 Ammonium glycyrrhizinate 0.1 Extract from Production Example 1 2.0 Lactic acid fermented rice 2.0 Hydrogenated lecithin 0.5 Hydrogenated lysolecithin 0.5 Oil-soluble ginseng extract 2.0 Xanthan gum 1.0 Zinc oxide 0.5 dl-camphor 0.3 l-menthol 0.5 Purified water, in an amount that makes the total volume 100 parts.

[0063] Example 9. Pack [Ingredients] Part Dipropylene glycol 5.0 Polyoxyethylene (60) hydrogenated castor oil 5.0 Cetanol 3.0 Behenyl alcohol 3.0 Allantoin 0.1 Dipotassium glycyrrhizinate 0.1 Ammonium glycyrrhizinate 0.1 β-Glycyrrhetinic acid 0.1 Stearyl glycyrrhetinate 0.1 Salicylic acid 0.1 Tocopherol acetate 0.5 Tocopherol nicotinate 0.1 D-Pantothenyl alcohol 0.3 Resorcinol 0.1 Sulfur 2.0 Estradiol 0.002 Extract from Production Example 2 1.0 Xanthan gum 2.0 Polyglyceryl-6 myristate 1.0 Potassium cocoyl glutamate 1.0 Hydrogenated lecithin 3.0 Hydroxylated lecithin 3.0 Purified water, in an amount that makes the total volume 100 parts.

[0064] Prescription Example 10: Hair Shampoo [Ingredients] Part Sodium laureth sulfate 10.0 Glyceryl monostearate 1.0 Coconut oil fatty acid diethanolamide 2.0 Polyoxyethylene (40) hydrogenated castor oil 0.5 Benzalkonium chloride 1.0 Stearyl alcohol 2.0 Behenyl alcohol 2.0 Dimethicone 3.0 Extract from Production Example 1 2.0 Allantoin 0.1 Dipotassium glycyrrhizinate 0.1 Salicylic acid 0.1 Sodium salicylate 0.1 Tocopherol acetate 0.1 Pyrithione Zinc 0.3 Benzoic acid 0.2 Triclosan 0.2 Citric acid 0.1 Propylene glycol 2.0 Purified water, in an amount that makes the total volume 100 parts.

[0065] Example 11. Hair Conditioner [Ingredients] Part Polyoxyethylene (10) hydrogenated castor oil 1.0 Distearyldimethylammonium chloride 1.5 Stearyltrimethylammonium chloride 2.0 Glyceryl 2-ethylhexanoate 1.0 Benzalkonium chloride 1.0 Cetanol 3.0 Stearyl alcohol 1.0 Extract from Production Example 1 2.0 Allantoin 0.1 Isopropylmethylphenol 0.1 Dipotassium glycyrrhizinate 0.1 Salicylic acid 0.1 Sulfur 0.5 Alkylisoquinolinium bromide solution (75%) 0.06 Pyrithione Zinc 0.3 Methylparaben 0.1 Triclosan 0.2 Resorcinol 0.1 Purified water, in an amount that makes the total volume 100 parts.

[0066] Prescription Example 11: Cleansing Cosmetics [Ingredients] Part Potassium cocoyl glycine 5.0 Glycerin 10.0 Glyceryl caprylate 1.0 Sodium lauroyl aspartate 10.0 Extract from Production Example 1: 1.0 Cetanol 3.0 Myristyl alcohol 3.0 Isopropylmethyl alcohol 0.1 Allantoin 0.1 Sulfur 0.5 Glycyrrhizic acid 0.1 Dipotassium glycyrrhizinate 0.1 Monoammonium glycyrrhizinate 0.1 β-Glycyrrhetinic acid 0.05 Stearyl glycyrrhetinate 0.1 Salicylic acid 0.2 Tocopherol acetate 0.2 Triclosan 0.1 Trichlorocarbanide 0.5 Trichlorohydroxydiphenyl ether 0.2 Concentrated benzalkonium chloride solution 50 0.2 Benzalkonium chloride 0.1 Purified water, in an amount that makes the total volume 100 parts.

[0067] Prescription Example 13: Sheet Mask A sheet mask is obtained by impregnating a nonwoven fabric with the following ingredients. [Ingredients] Part Extract from Production Example 1 2.0 Glycerin 3.0 1,3-Butylene glycol 2.0 L-ascorbic acid 2-glucoside 2.0 Methylparaben 0.2 Citric acid 0.1 Sodium citrate 0.3 Xanthan gum 1.0 Water-soluble collagen 1.0 Sodium hyaluronate 1.0 Seagrass extract 1.0 Rice extract hydrolyzate 1.0 Potassium hydroxide (appropriate amount) Purified water, in an amount that makes the total volume 100 parts.

[0068] Prescription Example 14: Serum [Ingredients] Part Ethanol 2.0 Glycerin 5.0 1,3-Butylene glycol 5.0 Methylparaben 0.1 Hyaluronic acid hydrolysate 1.0 Lactic acid bacteria culture 1.0 Extract from Production Example 2 2.0 Citric acid 0.3 Sodium citrate 0.6 Purified water, in an amount that makes the total volume 100 parts.

[0069] Prescription examples 15 Wrinkle-improving cream [Ingredients] Part Olive oil 5.0 Squalane 5.0 Jojoba oil 5.0 Jojoba wax 1.0 Shea butter 2.0 Behenyl alcohol 1.0 Stearyl alcohol 1.5 Candelilla wax 0.5 Niacinamide 5.0 Extract from Production Example 1: 0.2 Lactic acid fermented rice 3.0 Hydrogenated lecithin 2.0 Katamenkirinsai extract 2.0 Carboxyvinyl polymer 0.3 Sodium alginate 0.2 Glycerin 4.0 Potassium hydroxide (appropriate amount) Purified water, in an amount that makes the total volume 100 parts.

Claims

1. A collagen synthesis promoter in a topical skin preparation containing water of green rooibos, a mixed solvent of water and a polyhydric alcohol, or a mixed solvent of water and a lower alcohol.

2. A neutrophil-like cell migration activity inhibitor in a topical skin preparation containing water from green rooibos, a mixed solvent of water and a polyhydric alcohol, or a mixed solvent of water and a lower alcohol.

3. A topical skin preparation for promoting collagen synthesis or inhibiting neutrophil-like cell migration activity, comprising the agent described in Claim 1 or 2.

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